CN202837029U - Cell biology reactor sampler - Google Patents
Cell biology reactor sampler Download PDFInfo
- Publication number
- CN202837029U CN202837029U CN201220464779.9U CN201220464779U CN202837029U CN 202837029 U CN202837029 U CN 202837029U CN 201220464779 U CN201220464779 U CN 201220464779U CN 202837029 U CN202837029 U CN 202837029U
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- Prior art keywords
- sample
- stopple coupon
- pipe
- bioreactor
- sample introduction
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Abstract
The utility model discloses a cell biology reactor sampler. The cell biology reactor sampler comprises a sampling tube, a sample introduction tube, a sample introduction valve, air filters and an air pump. The sampling tube comprises a tube body and a top cap. A liquid inlet and an air port are formed in the top cap. Two ends of the sample introduction tube are respectively a sample introduction end and a sample discharge end. A sample introduction end port is connected with the liquid inlet of the top cap. The sample discharge end is used for being connected with a liquid outlet of a cell biology reactor. The sample introduction valve is arranged on the sample introduction tube. The sample introduction valve is opened or closed so that a pipeline between the sampling tube and the cell biology reactor is communicated or disconnected with a communicating pipeline. The air pump is connected with the air port of the top cap through one of the air filters. The sampler can carry out sampling in a one-hundred-grade aseptic environment, and besides, nutrient solution residue is avoided so that the sampler can be prevented form being contaminated by infectious microbes in sampling for a next time.
Description
Technical field
The utility model relates to a kind of bioreactor sampler.
Background technology
In the process of applying biological reactor fermented and cultured cell, need to carry out the sampling of nutrient solution, in case observation of cell or measure Related Component content in the nutrient solution, and then judge the growth conditions of cell, for next step operation provides foundation.The process of sampling must guarantee integral asepsis, otherwise can cause the reactor pollution microbes, makes and ferments unsuccessfully.At present, through type sampler commonly used operates in the industry, and normally reactor end connects the silicone tube of one section Band clamp, a terminal sample hose or other sample connections of connecting of silicone tube.During use, after using spirit lamp flame calcination end under hundred grades of clean environments, open clip, nutrient solution flows out from reactor along the sampler pipeline, collects sample.Use again the calcination of spirit lamp flame terminal after the end, guarantee terminal aseptic.Although easy and simple to handle, sampling process is extensive, the residual nutrient solution of meeting in the sampling line, easily microbiological contamination.
The utility model content
The purpose of this utility model aims to provide a kind of bioreactor sampler, and this sampler simple in structure can be taken a sample under hundred grades of gnotobasiss, and residual without nutrient solution, avoids being subject to when taking a sample living contaminants next time.
The purpose of this utility model realizes by following technical measures: a kind of bioreactor sampler, comprise stopple coupon, sample feeding pipe, the sample introduction valve, air strainer and air pump, described stopple coupon comprises body and top cover, described top cover is provided with inlet and gas port, described sample feeding pipe two ends are respectively sample introduction end and sample outlet end, described sample introduction port is connected in the inlet of described top cover, described sample outlet end then is used for being connected with the bioreactor liquid outlet, described sample introduction valve is arranged on the sample feeding pipe, opening or close the sample introduction valve makes the pipeline connection between described stopple coupon and the bioreactor or disconnects connecting pipeline, described air pump then is connected with gas port on the top cover by described air strainer, described air pump is adjusted the pressure reduction between stopple coupon and the bioreactor, nutrient solution is flow to the stopple coupon and when sampling finishes through described sample feeding pipe from the bioreactor outflow make the nutrient solution in the sample feeding pipe be back to bioreactor.Particularly, open the pipeline between described sample introduction valve connection stopple coupon and the bioreactor, air pump is bled, form negative pressure in the stopple coupon, be malleation in the bioreactor at this moment, nutrient solution flows to the stopple coupon through described sample feeding pipe from the liquid outlet outflow of bioreactor, after the amount of nutrient solution in the stopple coupon reaches sampling amount, air pump in the stopple coupon air inlet to the stopple coupon pressure greater than the pressure in the bioreactor, nutrient solution in the sample feeding pipe all is back in the bioreactor, close the sample introduction valve and disconnect connecting pipeline, finish sampling.
The utility model can be done following improvement: described sampler also comprises the sample pipe, describedly go out the two ends of sample pipe respectively as sample introduction end and sample outlet end, establish liquid outlet on the top cover of described stopple coupon, the described sample introduction end that goes out the sample pipe passes described liquid outlet and extends between bottom in the described stopple coupon and this end port and the bottom and leave the gap that the confession nutrient solution enters, described sample outlet end is provided with the joint that is connected for sample hose, the air inlet in the stopple coupon of described air pump, overpressure increases, the nutrient solution print enters out the sample pipe from the sample introduction end that goes out the sample pipe in the pipe, and flow out from sample outlet end and to enter the sample hose, and then detect.
The utility model also can further improve: described stopple coupon bottom is from inside to outside at the bottom of the spill camber, and the pipe shaft of the described sample introduction end that goes out the sample pipe is crooked, and the lowermost portion of the part edge of this end port bottom described stopple coupon contacts.The stopple coupon bottom is arcuate bottom, and nutrient solution can compile in the bottom, and when increasing the stopple coupon internal pressure, nutrient solution can all be discharged from, and can not remain in the sampler.
As an embodiment of the invention: that section pipe of the described sample outlet end that goes out the sample pipe is for having the high temperature resistant silicon sebific duct, described high temperature resistant silicon sebific duct can be crooked arbitrarily with stopple coupon bottom contact the time, to adjust the gap length between sample introduction port and the stopple coupon bottom.
Described in the utility modelly go out the sample pipe and be provided with the sample output valve door, valve is in closed condition when not using, avoid the living contaminants stopple coupon, when going out sample, open out the sample output valve door of sample pipe, the sample hose that is communicated with stopple coupon and detecting instrument goes out and closes the sample output valve door after sample finishes, and avoids being subject in the stopple coupon living contaminants.
Stopple coupon described in the utility model is provided with scale, can carry out quantitative sampling.
The volume of stopple coupon described in the utility model is not more than 20ml, otherwise the operation easier increase is difficult to realize sampling.Described stopple coupon can be high-temperature resistant glass tube or high-temperature resistance plastice pipe.
Sample feeding pipe described in the utility model and go out the sample pipe and can adopt high temperature resistant silicon sebific duct or stainless steel tubule.
As an embodiment of the present utility model: describedly go out the sample pipe and consisted of by the high temperature resistant silicon sebific duct that the two ends of stainless steel tubule and this stainless steel tubule connect, wherein the high temperature resistant silicon sebific duct that connects of an end is shorter for described stainless steel tubule, as the sample introduction end that goes out the sample pipe, described high temperature resistant silicon sebific duct can be crooked arbitrarily with stopple coupon bottom contact the time, to adjust the position relationship between sample introduction port and the stopple coupon bottom, the high temperature resistant silicon sebific duct that the other end connects is longer, the other end of this high temperature resistant silicon sebific duct is used for connecting the sample joint as sample outlet end.
In the utility model, when sample feeding pipe with when going out the sample pipe and being the high temperature resistant silicon sebific duct, described sample introduction valve and sample output valve door adopt clip.
Compared with prior art, the utlity model has following beneficial effect:
(1)The utility model arranges stopple coupon, and this stopple coupon is connected by sample feeding pipe with bioreactor, and by the bleeding or the operation of air inlet of air pump, changes the pressure reduction between stopple coupon and the bioreactor, realizes sampling.And when sampling finished, the air by in the compression stopple coupon was back in the reactor nutrient solution in the sample feeding pipe, so that do not have nutrient solution residual in the sample feeding pipe, and the sealing sampling valve is behind the door, can avoid the pollution of miscellaneous bacteria.
The utility model also sets out the sample pipe, and in experimental implementation, the backward detecting instrument sample introduction of taking a sample first can be delivered to the nutrient solution sample in the instrument fully, and does not have nutrient solution residual in the sampler.
Of the present utility modelly go out sample pipe bottom and be arranged to arcuate bottom, and the sample introduction end that goes out the sample pipe is crooked, and the port section edge contacts with the lowermost portion of arcuate bottom, during outside stock layout liquid, first the liquid of bottom is discharged, liquid levels constantly descends, and collects in the stopple coupon bottom, thereby with noresidue in the whole discharges of nutrient solution sample liquid and the stopple coupon.
The sample outlet end that goes out the sample pipe of the present utility model is the high temperature resistant silicon sebific duct, and silicone tube has pliability, can become bending under stressing conditions, need not to adopt the special glass pipe, makes things convenient for the assembling of sampler.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment.
Embodiment
Bioreactor sampler shown in Figure 1 is an embodiment of the present utility model, by stopple coupon 1, sample feeding pipe 2, sample introduction valve 3, go out sample pipe 4, sample output valve door 5, air strainer 6 and air pump 7 and consist of.Stopple coupon 1 is made of body 11 and top cover 12.Its top cover 12 is provided with inlet, liquid outlet and gas port for the threeway lid.Body 11 adopts pyrocerams or high-temperature resistance plastice pipe to make, and its pipe shaft is cylindrical, and the bottom is from inside to outside at the bottom of the spill camber.The body 11 of stopple coupon 1 is provided with for quantitative scale, and its total measurement (volume) is no more than 20ml.Sample feeding pipe 2 is the high temperature resistant silicon sebific duct, and one port is connected in the inlet of stopple coupon 1, and the inlet that passes stopple coupon 1 extends the top of stopple coupon, as sample outlet end.The other end then is used for being connected with the bioreactor liquid outlet, as the sample introduction end.Sample introduction valve 3 is a clip, is clipped on sample feeding pipe 2 pipe shafts, makes sampler sample introduction one end be closed condition.Going out sample pipe 4 connects respectively the high temperature resistant silicon sebific duct by stainless steel tubule 41 two ends and consists of, wherein the high temperature resistant silicon sebific duct 42 of end connection is shorter, and as the sample introduction end, the high temperature resistant silicon sebific duct 43 that the other end connects is longer, the end of this silicone tube is used for the joint that is connected with the EP sample hose as sample outlet end.Sample output valve door 5 is arranged on this long silicone tube, and sample output valve door 5 is clip.Go out the stainless steel tubule 41 of sample pipe 4 and extend in the stopple coupon 1 as the liquid outlet that the high temperature resistant silicon sebific duct 42 of sample introduction end passes stopple coupon, and the port of high temperature resistant silicon sebific duct contacts with the bottom of stopple coupon 1, and arbitrarily crooked when stressed, the part edge of the port of sample introduction end 2 contacts with the lowermost portion of the arcuate bottom of stopple coupon when bending, when stock layout outward, nutrient solution all can be discharged noresidue in the stopple coupon.Air pump 7 is connected with gas port on the top cover 12 by air strainer 6, by air pump 7 bleed and intake operation changes the interior pressure of stopple coupon 1, realize the sampling of nutrient solution and the nutrient solution sample discharged.
Sampler and cell reactor are placed hundred grades of gnotobasiss, connect pipeline between the two.When sampling, close out the sample output valve door 5 of sample pipe 4, namely clip is going out on the high temperature resistant silicon sebific duct 43 of sample pipe 4, closes out the pipeline between sample pipe 4 and the EP sample hose.Open the sample introduction valve 3 on the sample feeding pipe 2, namely take off the clip on the high temperature resistant silicon sebific duct, be communicated with the pipeline between bioreactor and the stopple coupon 1.When air pump 7 is bled, stopple coupon 7 interior formation negative pressure, be malleation in the bioreactor at this moment, nutrient solution flows to the stopple coupon 1 through sample feeding pipe 2 from the liquid outlet outflow of bioreactor, after the amount of nutrient solution in the stopple coupon 1 reaches sampling amount, air pump 7 in the stopple coupon 1 air inlet to the stopple coupon 1 pressure greater than the pressure in the bioreactor, nutrient solution in the sample feeding pipe 2 is back in the bioreactor, shut sample introduction valve 3, it is clip on the sample feeding pipe 2, close the pipeline between bioreactor and the stopple coupon 1, finish sampling.When going out sample, open out the sample output valve door 5 on the sample pipe 4, namely take off clip, air pump 7 increases the pressure in the stopple coupon 1 to stopple coupon 1 interior air inlet, and the nutrient solution sample enters out sample pipe 4, and drains in the EP sample hose.
The utility model can be summarized with other the concrete form without prejudice to spirit of the present utility model or principal character.Above-mentioned embodiment of the present utility model all can only be thought explanation of the present utility model rather than restriction, therefore every foundation essence technology of the present utility model all belongs in the scope of technical solutions of the utility model any trickle modification, equivalent variations and modification that above embodiment does.
Claims (10)
1. bioreactor sampler, it is characterized in that, comprise stopple coupon, sample feeding pipe, the sample introduction valve, air strainer and air pump, described stopple coupon comprises body and top cover, described top cover is provided with inlet and gas port, described sample feeding pipe two ends are respectively sample introduction end and sample outlet end, described sample introduction port is connected in the inlet of described top cover, described sample outlet end then is used for being connected with the bioreactor liquid outlet, described sample introduction valve is arranged on the sample feeding pipe, opening or close the sample introduction valve makes the pipeline connection between described stopple coupon and the bioreactor or disconnects connecting pipeline, described air pump then is connected with gas port on the top cover by described air strainer, described air pump is adjusted the pressure reduction between stopple coupon and the bioreactor, nutrient solution is flow to the stopple coupon and when sampling finishes through described sample feeding pipe from the bioreactor outflow make the nutrient solution in the sample feeding pipe be back to bioreactor.
2. bioreactor sampler according to claim 1, it is characterized in that, described sampler also comprises the sample pipe, describedly go out the two ends of sample pipe respectively as sample introduction end and sample outlet end, establish liquid outlet on the top cover of described stopple coupon, the described sample introduction end that goes out the sample pipe passes described liquid outlet and extends between bottom in the described stopple coupon and this end port and the bottom and leave the gap that the confession nutrient solution enters, described sample outlet end is provided with the joint that is connected for sample hose, the air inlet in the stopple coupon of described air pump increases overpressure, the nutrient solution print enters out the sample pipe from the sample introduction end that goes out the sample pipe in the pipe, and enters the sample hose from the sample outlet end outflow.
3. bioreactor sampler according to claim 2, it is characterized in that, described stopple coupon bottom is from inside to outside at the bottom of the spill camber, and the pipe shaft of the described sample introduction end that goes out the sample pipe is crooked, and the lowermost portion of the part edge of this end port bottom described stopple coupon contacts.
4. bioreactor sampler according to claim 2, it is characterized in that, that section pipe of the described sample outlet end that goes out the sample pipe is for having the high temperature resistant silicon sebific duct, described high temperature resistant silicon sebific duct can be crooked arbitrarily with stopple coupon bottom contact the time, to adjust the gap length between sample introduction port and the stopple coupon bottom.
5. according to claim 2 or 3 described bioreactor sampler, it is characterized in that, describedly go out the sample pipe and consisted of by the high temperature resistant silicon sebific duct that the two ends of stainless steel tubule and this stainless steel tubule connect, wherein the high temperature resistant silicon sebific duct that connects of an end is shorter for described stainless steel tubule, as the sample introduction end that goes out the sample pipe, described high temperature resistant silicon sebific duct can be crooked arbitrarily with stopple coupon bottom contact the time, to adjust the position relationship between sample introduction port and the stopple coupon bottom, the high temperature resistant silicon sebific duct that the other end connects is longer, the other end of this high temperature resistant silicon sebific duct is used for connecting the sample joint as sample outlet end.
6. according to claim 1 and 2 or 3 or 4 described bioreactor sampler, it is characterized in that the volume of described stopple coupon is not more than 20ml.
7. bioreactor sampler according to claim 6 is characterized in that, described stopple coupon is provided with for quantitative scale.
8. according to claim 2 or 3 described bioreactor sampler, it is characterized in that, describedly go out the sample pipe and be provided with the sample output valve door.
9. according to claim 2 or 3 described bioreactor sampler, it is characterized in that described to go out the sample pipe be high temperature resistant silicon sebific duct or stainless steel tubule.
10. bioreactor sampler according to claim 1 is characterized in that, described sample feeding pipe is high temperature resistant silicon sebific duct or stainless steel tubule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201220464779.9U CN202837029U (en) | 2012-09-13 | 2012-09-13 | Cell biology reactor sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201220464779.9U CN202837029U (en) | 2012-09-13 | 2012-09-13 | Cell biology reactor sampler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202837029U true CN202837029U (en) | 2013-03-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201220464779.9U Expired - Lifetime CN202837029U (en) | 2012-09-13 | 2012-09-13 | Cell biology reactor sampler |
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| Country | Link |
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| CN (1) | CN202837029U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107748583A (en) * | 2017-11-17 | 2018-03-02 | 华南理工大学 | A kind of closed temperature-controlling reaction apparatus of batch-type |
| CN108854883A (en) * | 2018-07-09 | 2018-11-23 | 马伟超 | A kind of chemical reaction system and its working method with monitor terminal |
| CN108854882A (en) * | 2018-07-09 | 2018-11-23 | 马伟超 | A kind of chemical reaction operating system and its working method that convenience remotely controls |
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
| CN113549536A (en) * | 2021-07-13 | 2021-10-26 | 深圳赛桥生物创新技术有限公司 | Sterile cell sampling device and method based on totally-enclosed syringe |
| CN113621501A (en) * | 2021-08-20 | 2021-11-09 | 华东理工大学青岛创新研究院 | Sterile sampling device and sampling method |
-
2012
- 2012-09-13 CN CN201220464779.9U patent/CN202837029U/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107748583A (en) * | 2017-11-17 | 2018-03-02 | 华南理工大学 | A kind of closed temperature-controlling reaction apparatus of batch-type |
| CN108854883A (en) * | 2018-07-09 | 2018-11-23 | 马伟超 | A kind of chemical reaction system and its working method with monitor terminal |
| CN108854882A (en) * | 2018-07-09 | 2018-11-23 | 马伟超 | A kind of chemical reaction operating system and its working method that convenience remotely controls |
| CN108854882B (en) * | 2018-07-09 | 2020-12-18 | 山东信发化工有限公司 | Chemical reaction operation system convenient for remote control and working method thereof |
| CN108854883B (en) * | 2018-07-09 | 2020-12-18 | 山东信发化工有限公司 | Chemical reaction system with monitoring terminal and working method thereof |
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
| CN113549536A (en) * | 2021-07-13 | 2021-10-26 | 深圳赛桥生物创新技术有限公司 | Sterile cell sampling device and method based on totally-enclosed syringe |
| WO2023284580A1 (en) * | 2021-07-13 | 2023-01-19 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
| CN113621501A (en) * | 2021-08-20 | 2021-11-09 | 华东理工大学青岛创新研究院 | Sterile sampling device and sampling method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 527400 9 East Causeway North Road, Xinxing County new town, Yunfu, Guangdong Patentee after: WENS FOODSTUFF GROUP Co.,Ltd. Address before: 527400 Yunfu, Xinxing County, Guangdong Patentee before: GUANGDONG WENS FOODSTUFF GROUP Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130327 |