CN201284337Y - Culture bottle with ventilating device - Google Patents
Culture bottle with ventilating device Download PDFInfo
- Publication number
- CN201284337Y CN201284337Y CN 200820072685 CN200820072685U CN201284337Y CN 201284337 Y CN201284337 Y CN 201284337Y CN 200820072685 CN200820072685 CN 200820072685 CN 200820072685 U CN200820072685 U CN 200820072685U CN 201284337 Y CN201284337 Y CN 201284337Y
- Authority
- CN
- China
- Prior art keywords
- bottle
- flask
- chamber
- ventpipe
- breathing pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The utility model discloses a culture flask with an air breather, comprising a flask body and a flask nozzle. The flask nozzle is provided with a flask stopper; a breathing pipe passes through the flask stopper, enters the flask body and is provided with a buffering chamber; a filtering chamber is arranged above the buffering chamber and at the outer part of the breathing pipe and the flask body; the filtering chamber is internally provided with a filtering net; an upper opening of the breathing pipe is provided with a sealing cover; as the filtering chamber is arranged, the filtering net is arranged in the filtering chamber and the upper opening of the breathing pipe is provided with the sealing cover, sampling can be carried out by an injector; a lower opening of the breathing pipe is close to the bottom of the flask but is not contacted with the bottom of the flask with good ventilation effect; the breathing pipe is provided with the buffering chamber, thus avoiding the culture solution from reversely flowing to the filtering chamber due to the syphonage and the change of the pressure in the culture flask so as to pollute the culture; the buffering chamber is arranged at the outside of the flask body and an air inlet of the buffering is provided with a check valve, and the buffering chamber can also be used as an air charging device. The culture flask has convenient and flexible ventilation and sampling, does not have strict requirements on the environment and can effectively avoid pollution from other bacteria.
Description
Technical field
The utility model relates to a kind of liquid bacterial and cultivates utensil, exactly is the culturing bottle of band breather.
Background technology
No matter be the check to bacterium in food, tap water, the pathological material of disease, during still to special physiological property (the non-shape etc. of cultivating of the bacteria in viable) research of bacterium, all will use liquid nutrient medium to increase bacterium earlier, some bacterium also needs aerated culture to increase bacterium.Simultaneously, also will to number of bacteria, form etc. take a sample the counting and microscopic examination.At present, because the condition for surveys difference, the instrument and equipment that is made also is not quite similar.But the method that relates to bacterium aerated culture and sampling all is to utilize traditional experiment utensil, as Erlenmeyer flask, fluid infusion bottle, test tube, guncotton plug etc.Specific practice is: at first the guncotton plug is filled in the Erlenmeyer flask (test tube or fluid infusion bottle etc.) that bacterial liquid is housed, ventilated by guncotton consent crack.Put it into static or rotational oscillation cultivation in the incubator then.Then need under aseptic condition plug be gone out during sampling, sample reveals empty, with the sterilization pipettor bacteria samples is shifted out again.Cultivate like this with sampling mode and be unfavorable for the bacterium breeding of increasing in a large number, in operating process, be not subjected to the mix pollution of bacterium of surrounding enviroment unavoidably.
Summary of the invention
The purpose of this utility model is, a kind of bacterium that is applicable to liquid culture and needs ventilation is provided, and is convenient, flexible in ventilation, sampling process, can avoid the band breather culturing bottle of living contaminants again.
For achieving the above object, the utility model is by the following technical solutions:
It comprises: be provided with bottle stopper in bottle, bottleneck, the bottleneck, ventpipe passes bottle stopper and enters in the bottle, it is characterized in that: ventpipe is provided with surge chamber, and above surge chamber, ventpipe bottle outside part has the filtration chamber, and filtering net is arranged in the filtration chamber.
At the bottom of described ventpipe end opening is pressed close to bottle but do not contact with at the bottom of the bottle;
Ventpipe is suitable for reading sealing cover;
Surge chamber is in bottle, near bottleneck;
Described surge chamber can also be located at outside the bottle, and the surge chamber inlet mouth is provided with reverse checkvalve.
During use, open sealing cover, with syringe by ventpipe suitable for reading in culturing bottle injecting gas, build sealing cover after having annotated gas; During sampling, use syringe, pierce through bottle stopper and enter bottle, draw samples.If surge chamber is located at outside the bottle, open sealing cover, the extruding surge chamber injects air in bottle, block surge chamber bottom ventpipe then, stops to push surge chamber, and reverse checkvalve is opened, and gas enters surge chamber, treats that surge chamber resiles behind the shape, unclamps ventpipe; During sampling, use syringe, pierce through bottle stopper and enter bottle, draw samples.
The utility model has filtering net owing to be provided with the filtration chamber in the filtration chamber, ventpipe is suitable for reading sealing cover, so available syringe sampling; At the bottom of the ventpipe end opening is pressed close to bottle but do not contact with at the bottom of the bottle, ventilatory effect is better; Ventpipe is provided with surge chamber, avoided nutrient solution because of the variation adverse current of siphonage and culturing bottle internal pressure to the filtration chamber, to such an extent as to culture pollutes; Surge chamber is located at outside the bottle, and the surge chamber inlet mouth is provided with reverse checkvalve, also can make inflation mechanism and use.It is convenient, flexible to ventilate, take a sample, not strict to environmental requirement, can avoid living contaminants effectively.
Description of drawings
Fig. 1 is a sectional view of the present utility model.
Fig. 2 is the sectional view of the another embodiment of the utility model.
Embodiment
Embodiment 1: see also Fig. 1, the utility model band breather culturing bottle, comprise be provided with bottle stopper 2 in bottle 1, bottleneck, the bottleneck, ventpipe 3 passes bottle stopper 2 and enters in the bottle 1, ventpipe 3 is provided with surge chamber 4, surge chamber 4 is in bottle 1, near bottleneck, above surge chamber 4, ventpipe 3 bottles 1 outside part has filtration chamber 5, and filtering net 51 is arranged in the filtration chamber 5, at the bottom of ventpipe 3 end openings are pressed close to bottle but do not contact with at the bottom of the bottle, ventpipe suitable for reading 6 has sealing cover 7.
During use, open sealing cover 7, with syringe by ventpipe 3 suitable for reading 6 injecting gas in culturing bottle, annotated gas after, build sealing cover 7; During sampling, use syringe, pierce through bottle stopper 2 and enter bottle 1, draw samples.
Embodiment 2: see also accompanying drawing 2, the utility model band breather culturing bottle, comprise be provided with bottle stopper 2 in bottle 1, bottleneck, the bottleneck, ventpipe 3 passes bottle stopper 2 and enters in the bottle 1, ventpipe 3 is provided with surge chamber 4, and surge chamber 4 is outside bottle 1, and surge chamber 4 inlet mouths are provided with reverse checkvalve 8, there is filtration chamber 5 surge chamber 4 tops, filtering net 51 is arranged in the filtration chamber 5, and at the bottom of ventpipe 3 end openings are pressed close to bottle but do not contact with at the bottom of the bottle, ventpipe 3 suitable for reading 6 has sealing cover 7.
Open sealing cover 7 during use, extruding surge chamber 4, reverse checkvalve 8 is closed, injecting gas in bottle 1, block surge chamber 4 bottom ventpipes 3 then, stop to push surge chamber 4, reverse checkvalve 8 is opened, gas enters surge chamber 4, treats that surge chamber 4 resiles behind the shape, unclamps ventpipe 3; After having annotated body, build sealing cover 7; During sampling, use syringe, pierce through bottle stopper 2 and enter bottle 1, draw samples.
Claims (5)
1, band breather culturing bottle, it comprises: be provided with bottle stopper in bottle, bottleneck, the bottleneck, ventpipe passes bottle stopper and enters in the bottle, it is characterized in that: ventpipe is provided with surge chamber, above surge chamber, ventpipe bottle outside part has the filtration chamber, and filtering net is arranged in the filtration chamber.
2, according to claim 1 band breather culturing bottle, it is characterized in that: at the bottom of described ventpipe end opening is pressed close to bottle but do not contact with at the bottom of the bottle.
3, according to claim 2 band breather culturing bottle, it is characterized in that: ventpipe is suitable for reading sealing cover.
4, according to claim 1,2 or 3 band breather culturing bottles, it is characterized in that: surge chamber is in bottle, near bottleneck.
5, according to claim 1,2 or 3 band breather culturing bottles, it is characterized in that: described surge chamber is located at outside the bottle, and the surge chamber inlet mouth is provided with reverse checkvalve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820072685 CN201284337Y (en) | 2008-10-31 | 2008-10-31 | Culture bottle with ventilating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820072685 CN201284337Y (en) | 2008-10-31 | 2008-10-31 | Culture bottle with ventilating device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201284337Y true CN201284337Y (en) | 2009-08-05 |
Family
ID=40949326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200820072685 Expired - Fee Related CN201284337Y (en) | 2008-10-31 | 2008-10-31 | Culture bottle with ventilating device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201284337Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123527A (en) * | 2015-09-17 | 2015-12-09 | 山东省农作物种质资源中心 | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium |
-
2008
- 2008-10-31 CN CN 200820072685 patent/CN201284337Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123527A (en) * | 2015-09-17 | 2015-12-09 | 山东省农作物种质资源中心 | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090805 Termination date: 20091130 |