CN215050239U - Bioreactor aseptic sampling kit - Google Patents
Bioreactor aseptic sampling kit Download PDFInfo
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- CN215050239U CN215050239U CN202121123625.9U CN202121123625U CN215050239U CN 215050239 U CN215050239 U CN 215050239U CN 202121123625 U CN202121123625 U CN 202121123625U CN 215050239 U CN215050239 U CN 215050239U
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Abstract
The utility model discloses a bioreactor sterile sampling kit, which comprises an injector, a transfer bottle, a sample bottle, a waste liquid bottle, a fixed bracket and a reactor, wherein the transfer bottle, the sample bottle and the waste liquid bottle are fixed on the fixed bracket; the transfer bottle is connected to the sample bottle through a silica gel hose, is provided with a tee joint on the silica gel hose and is connected to a waste liquid bottle through the silica gel hose, and the transfer bottle is connected to the reactor through the silica gel hose simultaneously, the silica gel hose is provided with a reactor pipe clamp. The utility model discloses compact structure, convenient operation can accomplish the aseptic sample of fermentation sample in the bioreactor in real time, and aseptic degree is high in the operation process, can effectively guarantee to take out the sample and avoid infecting of the miscellaneous fungus of environment, satisfies the analysis and the application demand to the sample in the follow-up technology.
Description
Technical Field
The utility model relates to a sampling device especially relates to a bioreactor aseptic sample external member.
Background
Bioreactors are the primary driver to advance the bioproduct industry. Before any biological product is industrialized, the continuous amplification processes of strain screening, small test, pilot test, large production and the like are required. The bioreactor needs to sample at regular time to analyze and detect cell morphology, cell amount and metabolite in the process of culturing microorganisms and cells. Generally, the sampling is performed at least once a day, and in most cases, multiple times a day. In the conventional sampling, a sample is generally taken out through a mode of sucking by an injector or differential pressure at a port of a sampling pipe, the sample taking-out process is poor in aseptic operation, the taken-out sample is easily infected by environmental infectious microbes, and the use of the taken-out sample in a subsequent process with high requirements on cleanliness grade is directly influenced. Therefore, the development of a bioreactor sterile sampling kit is a problem to be solved by those skilled in the art.
Disclosure of Invention
The utility model provides a bioreactor aseptic sampling kit for solving the above-mentioned insufficiency, can effectively guarantee to take out the cleanliness factor of sample, does not receive infecting of the miscellaneous fungus of environment.
The above object of the present invention is achieved by the following technical solutions: a bioreactor sterile sampling kit comprises an injector, a transfer bottle, a sample bottle, a waste liquid bottle, a fixed support and a reactor, wherein the transfer bottle, the sample bottle and the waste liquid bottle are fixed on the fixed support; the transfer bottle is connected to the sample bottle through a silica gel hose, is provided with a tee joint on the silica gel hose and is connected to a waste liquid bottle through the silica gel hose, and the transfer bottle is connected to the reactor through the silica gel hose simultaneously, the silica gel hose is provided with a reactor pipe clamp.
Further, a syringe air filter is arranged between the syringe and the adapter bottle.
Further, the injector is a spiral hole injector, and one end of the injector air filter is directly butted with the injector.
Further, the syringe air filter is made of PTFE material, the diameter of the syringe air filter is phi 50mm, and the filtering pore diameter of the syringe air filter is 0.2 μm.
Furthermore, the adapter bottle comprises an adapter bottle body and an adapter bottle cap, three opening connecting pipes are arranged on the adapter bottle, namely an air pipe, a liquid pumping pipe and a sample collecting pipe, the sample collecting pipe extends to the bottom of the adapter bottle, and when the injector pumps air, air in the adapter bottle can be pumped out through a silica gel hose connected to the adapter bottle to form negative pressure, so that a sample in the bioreactor can be conveniently pumped into the adapter bottle through the liquid pumping pipe on the adapter bottle; when the syringe pushes away gas, can push away sterile air in the transfer bottle through connecting the silica gel hose on the transfer bottle, form the high pressure, push away the sample in the transfer bottle to waste liquid bottle or sample bottle through receiving the appearance pipe.
Furthermore, the sample bottle comprises a sample bottle body, a sample bottle cap, a sample bottle air filter, a sample bottle pipe clamp and a sample bottle luer connector, wherein the sample bottle cap is connected with the sample bottle air filter, so that the internal pressure of the bottle can be discharged and the bottle can be isolated from the outside in a sterile manner in the sampling process; a silica gel hose connected to the sample bottle is provided with a sample bottle pipe clamp and a sample bottle luer connector, so that a new sterile sample bottle can be rapidly and aseptically replaced in the sampling process; the sample bottle air filter is made of PTFE material, the diameter of the sample bottle air filter is phi 25mm, and the filtering aperture is 0.2 mu m; the sample bottle was made of borosilicate glass.
Further, the waste liquid bottle includes waste liquid bottle, waste liquid bottle lid, waste liquid bottle air cleaner, waste liquid bottle pipe clamp and waste liquid bottle luer connects, be connected with waste liquid bottle air cleaner on the waste liquid bottle lid, the in-process of the waste discharge of being convenient for, the emission of bottle internal pressure reaches and external aseptic isolation. A waste liquid bottle pipe clamp and a waste liquid bottle luer connector are arranged on the silica gel hose connected to the waste liquid bottle, so that the waste liquid bottle can be rapidly and aseptically replaced by a new sterile waste liquid bottle after the waste discharge pipe is filled; the waste liquid bottle air filter is made of PTFE material, the diameter of the waste liquid bottle air filter is phi 25mm, and the filtering aperture is 0.2 mu m; the waste liquid bottle is made of borosilicate glass.
Furthermore, the fixing support is provided with a transfer bottle fixing hole, a sample bottle fixing hole and a waste liquid bottle fixing hole, and the transfer bottle, the sample bottle and the waste liquid bottle are respectively fixed.
Furthermore, the number of the sample bottle fixing holes is 6, the sample bottle fixing holes are respectively used for fixing 6 sample bottles in a placing mode.
During sampling, all devices and pipelines except the injector need to be sterilized like the bioreactor before use. The sampling operation flow is as follows:
a. waste discharge operation: opening the pipe clamp, pulling the syringe to perform air exhaust operation, sucking the sample in the bioreactor into the transfer bottle, closing the pipe clamp, opening the waste liquid bottle pipe clamp on the waste liquid bottle, pushing the syringe to perform air exhaust operation, pushing the sample in the transfer bottle into the waste liquid bottle, closing the waste liquid bottle pipe clamp, and completing waste discharge operation.
b. Sampling operation: and opening the pipe clamp again, pulling the injector again to pump air, pumping the sample into the transfer bottle again, closing the pipe clamp, opening the pipe clamp of the sample bottle at the upper end of the sample bottle, pushing the injector again to inflate, pushing the sterile sample into the sample bottle, and closing the pipe clamp of the sample bottle to finish sampling operation once. When a sample is taken down, only the luer connector on the original sample bottle needs to be unscrewed under the protection of aseptic conditions to replace another aseptic sample bottle.
Compared with the prior art, the utility model the advantage be: the device is provided with a transfer bottle, a sample bottle and a waste liquid bottle, an air filter and a bioreactor are connected in series through a silica gel hose, the front end of the air filter is connected with an injector, and the injector can take out a sample in the bioreactor through push-pull action. The device has the advantages of compact structure and convenient operation, can finish the aseptic sampling of fermentation samples in the bioreactor in real time, has high aseptic degree in the operation process, can effectively ensure that the samples are taken out and are not infected by environmental mixed bacteria, and meets the analysis and application requirements of the samples in the subsequent process.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic view of a transfer bottle structure of the present invention.
Fig. 3 is a schematic diagram of the structure of the sample bottle of the present invention.
Fig. 4 is a schematic view of the structure of the waste liquid bottle of the present invention.
Fig. 5 is a schematic view of the hole structure of the middle fixing bracket of the present invention.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
As shown in fig. 1, a bioreactor sterile sampling kit comprises an injector 1, a transfer bottle 2, a sample bottle 3, a waste liquid bottle 4, a fixed bracket 5 and a reactor 6, wherein the transfer bottle 2, the sample bottle 3 and the waste liquid bottle 4 are fixed on the fixed bracket 5, the injector 1 is connected to the transfer bottle 2 through a silica gel hose S1, the pressure of the transfer bottle can be controlled by pushing and pulling the injector, and the suction and pushing of a sample are completed; the transfer bottle 2 is connected to the sample bottle 3 through a silica gel hose S3, is provided with a tee T1 on the silica gel hose S3 and is connected to the waste liquid bottle 4 through a silica gel hose, and simultaneously the transfer bottle 2 is connected to the reactor 6 through a silica gel hose S2, the silica gel hose S2 is provided with a reactor pipe clamp V1.
Further, a syringe air filter F1 is arranged between the syringe 1 and the adapter bottle 2.
Further, the syringe 1 is a screw hole syringe, and one end of the syringe air filter F1 is directly butted with the syringe.
Further, the syringe air filter F1 is made of PTFE material, the diameter of the syringe air filter F1 is phi 50mm, and the filter pore size thereof is 0.2 μm.
Further, as shown in fig. 1 and fig. 2, the adapter bottle 2 includes an adapter bottle body 2.1 and an adapter bottle cap 2.2, the adapter bottle 2 is provided with three open connection pipes, which are an air pipe 2.3, a liquid suction pipe 2.4 and a sample receiving pipe 2.5, the sample receiving pipe 2.5 extends to the bottom of the adapter bottle 2, when the syringe 1 is used for pumping air out, the air in the adapter bottle 2 can be pumped out through a silica gel hose connected to the adapter bottle 2 to form a negative pressure, so that a sample in the bioreactor 6 can be conveniently pumped into the adapter bottle 2 through the liquid suction pipe on the adapter bottle 2; when the injector 1 pushes air, sterile air can be pushed into the adapter bottle 2 through a silica gel hose connected to the adapter bottle 2 to form high pressure, and a sample in the adapter bottle 2 is pushed into the waste liquid bottle 4 or the sample bottle 3 through the sample receiving tube 2.5.
Further, as shown in fig. 1 and fig. 3, the sample bottle 3 includes a sample bottle body 3.1, a sample bottle cap 3.2, a sample bottle air filter 3.3, a sample bottle tube clamp 3.4 and a sample bottle luer 3.5, and the sample bottle cap 3.2 is connected with the sample bottle air filter 3.3, so as to facilitate the discharge of pressure in the bottle and the aseptic isolation from the outside during the sampling process; a sample bottle pipe clamp 3.4 and a sample bottle luer connector 3.5 are arranged on the silica gel hose S3 connected to the sample bottle 3, so that a new sterile sample bottle can be rapidly and aseptically replaced in the sampling process; the sample bottle air filter 3.3 is made of PTFE material, the diameter of the sample bottle air filter is phi 25mm, and the filtering aperture is 0.2 mu m; the sample bottle 3 is made of borosilicate glass.
Further, as shown in fig. 1 and 4, the waste liquid bottle 4 includes a waste liquid bottle body 4.1, a waste liquid bottle cap 4.2, a waste liquid bottle air filter 4.3, a waste liquid bottle pipe clamp 4.4 and a waste liquid bottle luer connector 4.5, and the waste liquid bottle cap 4.2 is connected with the waste liquid bottle air filter 4.3, so that the waste liquid is conveniently discharged and the pressure in the bottle is aseptically isolated from the outside in the waste discharge process. A waste liquid bottle pipe clamp 4.4 and a waste liquid bottle luer connector 4.5 are arranged on the silica gel hose S3 connected to the waste liquid bottle 4, so that the waste liquid bottle can be rapidly and aseptically replaced by a new sterile waste liquid bottle after the waste discharge pipe is filled; the waste liquid bottle air filter 4.3 is made of PTFE material, the diameter of the waste liquid bottle air filter is phi 25mm, and the filtering aperture is 0.2 mu m; the waste liquid bottle 4 is made of borosilicate glass.
Further, as shown in fig. 1 and 5, the fixing bracket 5 is provided with a transfer bottle fixing hole 5.1, a sample bottle fixing hole 5.2 and a waste liquid bottle fixing hole 5.3, and respectively fixes the transfer bottle 2, the sample bottle 3 and the waste liquid bottle 4.
Furthermore, the number of the sample bottle fixing holes is 6, the sample bottle fixing holes are 5.21-5.26 respectively, and 6 sample bottles can be placed and fixed at the same time.
As shown in FIGS. 1 to 5, all devices and lines except the syringe 1 need to be sterilized before use as a bioreactor. The sampling operation flow is as follows:
a. waste discharge operation: opening the pipe clamp V1, pulling the injector 1 to perform air suction operation, sucking the sample in the bioreactor 6 into the adapter bottle 2, then closing the pipe clamp V1, opening the waste liquid bottle pipe clamp 4.4 on the waste liquid bottle 4, pushing the injector 1 to perform air inflation operation, pushing the sample in the adapter bottle 2 into the waste liquid bottle 4, closing the waste liquid bottle pipe clamp 4.4, and completing waste discharge operation.
b. Sampling operation: opening the pipe clamp V1 again, pulling the syringe 1 again to pump air, pumping the sample into the adapter bottle 2 again, closing the pipe clamp V1, opening the sample bottle pipe clamp 3.4 at the upper end of the sample bottle 3, pushing the syringe 1 again to pump air, pushing the sterile sample into the sample bottle 3, closing the sample bottle pipe clamp 3.4 to finish one sampling operation. When a sample is taken down, only the luer connector 3.5 on the original sample bottle 3 needs to be unscrewed under the protection of aseptic conditions to replace another aseptic sample bottle.
The above only is the embodiment of the present invention, not limiting the patent scope of the present invention, all the equivalent structures or equivalent processes that are used in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the patent protection scope of the present invention.
Claims (9)
1. A bioreactor sterile sampling kit, characterized by: the device comprises an injector, a transfer bottle, a sample bottle, a waste liquid bottle, a fixed support and a reactor, wherein the transfer bottle, the sample bottle and the waste liquid bottle are fixed on the fixed support; the transfer bottle is connected to the sample bottle through a silica gel hose, is provided with a tee joint on the silica gel hose and is connected to a waste liquid bottle through the silica gel hose, and the transfer bottle is connected to the reactor through the silica gel hose simultaneously, the silica gel hose is provided with a reactor pipe clamp.
2. A bioreactor sterile sampling kit as set forth in claim 1, wherein: and a syringe air filter is arranged between the syringe and the adapter bottle.
3. A bioreactor sterile sampling kit as set forth in claim 1, wherein: the injector is a spiral hole injector, and one end of an injector air filter is directly butted with the injector.
4. A bioreactor sterile sampling kit as set forth in claim 2, wherein: the injector air filter is made of PTFE material, the diameter of the injector air filter is phi 50mm, and the filtering pore diameter of the injector air filter is 0.2 mu m.
5. A bioreactor sterile sampling kit as set forth in claim 1, wherein: the switching bottle comprises a switching bottle body and a switching bottle cap, three opening connecting pipes are arranged on the switching bottle and respectively comprise an air pipe, a liquid pumping pipe and a sample collecting pipe, and the sample collecting pipe extends to the bottom of the switching bottle.
6. A bioreactor sterile sampling kit as set forth in claim 1, wherein: the sample bottle comprises a sample bottle body, a sample bottle cap, a sample bottle air filter, a sample bottle pipe clamp and a sample bottle luer connector, wherein the sample bottle cap is connected with the sample bottle air filter, the sample bottle pipe clamp and the sample bottle luer connector are arranged on a silica gel hose connected to the sample bottle, the sample bottle air filter is made of PTFE material, the diameter of the sample bottle air filter is phi 25mm, and the filtering pore size is 0.2 mu m; the sample bottle was made of borosilicate glass.
7. A bioreactor sterile sampling kit as set forth in claim 1, wherein: the waste liquid bottle comprises a waste liquid bottle body, a waste liquid bottle cap, a waste liquid bottle air filter, a waste liquid bottle pipe clamp and a waste liquid bottle luer connector, the waste liquid bottle cap is connected with the waste liquid bottle air filter, the waste liquid bottle pipe clamp and the waste liquid bottle luer connector are arranged on a silica gel hose connected to the waste liquid bottle, the waste liquid bottle air filter is made of PTFE materials, the diameter of the waste liquid bottle air filter is phi 25mm, and the filtering aperture is 0.2 mu m; the waste liquid bottle is made of borosilicate glass.
8. A bioreactor sterile sampling kit as set forth in claim 1, wherein: the fixing support is provided with a transfer bottle fixing hole, a sample bottle fixing hole and a waste liquid bottle fixing hole, and the transfer bottle, the sample bottle and the waste liquid bottle are respectively fixed.
9. A bioreactor sterile sampling kit as set forth in claim 8, wherein: the sample bottle fixed orifices be 6, be the sample bottle fixed orifices respectively, can place fixed 6 sample bottles simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121123625.9U CN215050239U (en) | 2021-05-25 | 2021-05-25 | Bioreactor aseptic sampling kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121123625.9U CN215050239U (en) | 2021-05-25 | 2021-05-25 | Bioreactor aseptic sampling kit |
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| CN215050239U true CN215050239U (en) | 2021-12-07 |
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| CN202121123625.9U Active CN215050239U (en) | 2021-05-25 | 2021-05-25 | Bioreactor aseptic sampling kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114181807A (en) * | 2021-12-15 | 2022-03-15 | 迪必尔生物工程(上海)有限公司 | High-throughput sampling system and sampling method |
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2021
- 2021-05-25 CN CN202121123625.9U patent/CN215050239U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114181807A (en) * | 2021-12-15 | 2022-03-15 | 迪必尔生物工程(上海)有限公司 | High-throughput sampling system and sampling method |
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