CN219385102U - Automatic cell sorting system - Google Patents
Automatic cell sorting system Download PDFInfo
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- CN219385102U CN219385102U CN202223602318.6U CN202223602318U CN219385102U CN 219385102 U CN219385102 U CN 219385102U CN 202223602318 U CN202223602318 U CN 202223602318U CN 219385102 U CN219385102 U CN 219385102U
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- 239000007788 liquid Substances 0.000 claims abstract description 139
- 239000000872 buffer Substances 0.000 claims abstract description 84
- 238000001514 detection method Methods 0.000 claims description 24
- 239000011324 bead Substances 0.000 claims description 23
- 230000002572 peristaltic effect Effects 0.000 claims description 22
- 238000005086 pumping Methods 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 5
- 238000005096 rolling process Methods 0.000 abstract description 4
- 239000012530 fluid Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 239000002699 waste material Substances 0.000 description 8
- 238000007664 blowing Methods 0.000 description 7
- 210000001503 joint Anatomy 0.000 description 6
- 238000004806 packaging method and process Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 230000002209 hydrophobic effect Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000002659 cell therapy Methods 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 238000013459 approach Methods 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000005347 demagnetization Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000005251 gamma ray Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002906 medical waste Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
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Abstract
The utility model discloses an automatic cell sorting system which comprises a liquid containing unit, a buffer container, an air pump unit, a cell sorting unit and a liquid receiving unit, wherein the liquid containing unit is connected with a liquid inlet of the buffer container, a liquid outlet of the buffer container is connected with the liquid receiving unit through the cell sorting unit, and the air pump unit is communicated with the buffer container and is used for pumping or inflating the buffer container to realize inflow and discharge of liquid in the buffer container. According to the utility model, the buffer container is arranged between the liquid containing unit and the cell sorting unit, and then the air pump unit is used for pumping and inflating the buffer container, so that the liquid in the buffer container is controlled to flow in and drain out, the air pump unit is not contacted with cells, the transportation of the cells can be completed, the damage of roller rolling in the pump to the activity and the form of the cells is avoided, and the breakage rate of the cells is reduced.
Description
Technical Field
The utility model mainly relates to the technical field of medicine and food packaging machinery equipment, in particular to an automatic cell sorting system.
Background
At present, the cell therapy technology at home and abroad is developed rapidly, and cell therapy products are directly input into human bodies and cannot be subjected to terminal sterilization or other virus removal process treatment. In laboratories, research and development institutions and related production, common cells are centrifuged and then are directly separated in a magnetic field, other manual operations are performed, pollution to cell samples is easy to occur, and the yield and activity of target cells of the existing automatic separation equipment in the market are not high.
The applicant has filed patent applications (such as 202221782866.9) related to a cell sorting device to realize automatic sorting of cells to avoid pollution to cell samples caused by manual operation, but products corresponding to the patent applications find that certain cracks exist in the sorted cells in the testing and using processes. Further intensive studies have found that the reason for this is that the pump directly pumps and discharges the cell fluid, which in turn leads to cell disruption.
Disclosure of Invention
The technical problem to be solved by the utility model is as follows: aiming at the technical problems in the prior art, the utility model provides a cell automatic sorting system for reducing the cell breakage rate.
In order to solve the technical problems, the technical scheme provided by the utility model is as follows:
the automatic cell sorting system comprises a liquid containing unit, a buffer container, an air pump unit, a cell sorting unit and a liquid collecting unit, wherein the liquid containing unit is connected with a liquid inlet of the buffer container, a liquid outlet of the buffer container is connected with the liquid collecting unit through the cell sorting unit, and the air pump unit is communicated with the buffer container and is used for pumping or inflating the buffer container to realize inflow and discharge of liquid in the buffer container.
As a further improvement of the above technical scheme:
the liquid inlet and the liquid outlet of the buffer container are both positioned at the bottom of the buffer container, and the air pump unit is communicated with the top of the buffer container.
The buffer container is a dropping funnel.
The cell sorting unit comprises a plurality of cell sorting pipes, the inlets of the cell sorting pipes are connected with the liquid outlet of the buffer container, and the outlets of the cell sorting pipes are connected with the liquid collecting unit.
Each cell sorting tube is provided with a joint component for injecting magnetic beads.
The connector assembly comprises a connector and a cap body, the connector is positioned at the top of the cell sorting tube, and the cap body is covered on the connector.
The air pump unit is a peristaltic pump.
And a bubble sensor is arranged on a pipeline between the buffer container and the cell sorting unit.
The device also comprises a tightness detection unit for detecting tightness of a pipeline between a liquid outlet of the liquid containing unit and a liquid inlet of the liquid receiving unit.
The tightness detection unit comprises a pressurizing assembly and a pressure detection assembly, and the pressurizing assembly is used for filling pressurized gas into a pipeline between a liquid outlet of the liquid containing unit and a liquid inlet of the liquid receiving unit; the pressure detection component is used for detecting the pressure of a pipeline between a liquid outlet of the liquid containing unit and a liquid inlet of the liquid receiving unit in a preset time period after pressurization.
Compared with the prior art, the utility model has the advantages that:
according to the automatic cell sorting system, the buffer container is arranged between the liquid containing unit and the cell sorting unit, and then the air pump unit is used for carrying out air suction and air inflation operation on the buffer container, so that the liquid in the buffer container is controlled to flow in and flow out, the air pump unit is not contacted with cells, the transportation of the cells can be completed, the damage of roller rolling in the pump to the activity and the form of the cells is avoided, and the breakage rate of the cells is reduced.
According to the automatic cell sorting system, the liquid inlet and the liquid outlet of the buffer container are both positioned at the bottom of the buffer container, and the air pump unit is communicated with the top of the buffer container, so that the generation of bubbles in the buffer container is reduced, and the cell breakage rate is further reduced.
Drawings
Fig. 1 is a schematic diagram of a sorting system according to an embodiment of the present utility model.
Fig. 2 is a schematic structural view of a bottom connection port of a bag according to an embodiment of the present utility model.
Fig. 3 is a schematic structural diagram of connection between the tightness detecting unit and the pipeline.
FIG. 4 is a schematic diagram of the structure of an interface on a cell sorting tube according to the present utility model in an embodiment.
Legend description: 1. a liquid containing unit; 101. a cell fluid bag; 102. a buffer solution bag; 201 to 210, first to tenth normally closed pinch valves; 3. an air pump unit; 301. a peristaltic pump; 401. a bubble sensor; 5. a cell sorting unit; 501-503, first-third cell sorting tubes; 504. a joint assembly; 5041. a connector; 5042. a cap body; 601 to 615, first to fifteenth pipelines; 7. a buffer container; 701. a dropping funnel; 8. a liquid collecting unit; 801. a cell collection bag; 802. a waste liquid bag; 9. and a tightness detection unit.
Detailed Description
The utility model is further described below with reference to the drawings and specific examples.
As shown in fig. 1, the automatic cell sorting system according to the embodiment of the utility model comprises a liquid containing unit 1, a buffer container 7, an air pump unit 3, a cell sorting unit 5 and a liquid collecting unit 8, wherein the liquid containing unit 1 is connected with a liquid inlet of the buffer container 7, a liquid outlet of the buffer container 7 is connected with the liquid collecting unit 8 through the cell sorting unit 5, and the air pump unit 3 is communicated with the buffer container 7 and is used for pumping or inflating the buffer container 7 to realize inflow and discharge of liquid in the buffer container 7.
According to the automatic cell sorting system, the buffer container 7 is arranged between the liquid containing unit 1 and the cell sorting unit 5, and then the air pump unit 3 is used for pumping and inflating the buffer container 7, so that the inflow and the discharge of liquid in the buffer container 7 are controlled, the air pump unit 3 is not contacted with cells, the transportation of the cells can be completed, the damage of roller rolling in the pump to the activity and the form of the cells is avoided, and the breakage rate of the cells is reduced.
In a specific embodiment, the liquid inlet and the liquid outlet of the buffer container 7 are both located at the bottom of the buffer container 7, and the air pump unit 3 is communicated with the top of the buffer container 7, so as to reduce the generation of bubbles in the buffer container 7 and further reduce the cell breakage rate.
In a specific embodiment, the cell sorting unit 5 includes three cell sorting tubes, the inlets of the cell sorting tubes are connected to the liquid outlet of the buffer container 7, and the outlets of the cell sorting tubes are connected to the liquid receiving unit 8. Wherein each cell sorting tube 501 is provided with a connector assembly 504 for injecting magnetic beads. Specifically, the connector assembly 504 includes a connector 5041 and a cap 5042, the connector 5041 is located at the top of the cell sorting tube 501, and the cap 5042 is covered on the connector 5041. By the design of the joint component 504, magnetic beads can be quickly added into the biosafety cabinet, and pressure balance in the sorting tube can be maintained during liquid adding.
In a specific embodiment, the device further includes a tightness detecting unit 9, configured to detect tightness of a pipeline between the liquid outlet of the liquid containing unit 1 and the liquid inlet of the liquid receiving unit 8. Specifically, the tightness detection unit 9 comprises a pressurizing assembly and a pressure detection assembly, wherein the pressurizing assembly is used for filling pressurized gas into a pipeline between a liquid outlet of the liquid containing unit 1 and a liquid inlet of the liquid receiving unit 8; the pressure detection component is used for detecting the pressure of a pipeline between a liquid outlet of the liquid containing unit 1 and a liquid inlet of the liquid receiving unit 8 in a preset time period after pressurization. The tightness detection unit 9 is used for carrying out tightness detection on the sorting system, so that the reliability of subsequent work is ensured.
The specific structure of the sorting system of the present utility model will be described in detail with reference to the accompanying drawings:
and (3) liquid bag design:
the cell liquid bag 101, the buffer liquid bag 102, the cell collecting bag 801 and the waste liquid bag 802 are four liquid bags, are made of EVA, PVC or ULPED and other transparent soft plastic materials meeting biocompatibility, and are provided with three connecting ports at the bottom, as shown in figure 2. Specifically, the left connecting port is sequentially provided with the pipe clamp, the 0.22 mu m liquid filter (with the female connector) and the male cap, the male cap can be taken down in the biosafety cabinet, and the disposable sterile syringe with the male connector is used for adding the cell liquid sample, the buffer solution and the like into the liquid bag. The middle connecting port is sequentially provided with the pipe clamp, the female connector and the male cap, the male cap can be taken down in the biosafety cabinet, and the female connector of the biosafety cabinet is in butt joint with the male connector of the pipeline after the female cap is taken down. The pipe clamp, the female connector and the male cap are sequentially arranged on the right connecting port, the male cap can be taken down in the biosafety cabinet, and the female connector of the biosafety cabinet is in butt joint with the male connector of the pipeline after the female cap is taken down. The right port is a reserved interface.
And (3) pipeline design:
the pipeline is made of transparent plastic materials such as PVC or silicone tubes which meet biocompatibility;
the first pipe 601 and the second pipe 602 are respectively provided with a first pinch valve 201 and a second pinch valve 202, so that female caps on the first pipe 601 and the second pipe 602 can be taken down in the biosafety cabinet, and the male connectors of the female caps are butted with the female connectors of the cell fluid bag 101 and the buffer fluid bag 102 after the male caps are taken down, so that liquid adding is provided for a pipeline system.
The third pipeline 603 is a branch pipeline on the first pipeline 601 and is connected through a T-shaped three-way joint. As shown in fig. 3, a third pipeline 603 is provided with a pipe clamp, a 0.22 μm hydrophobic gas filter (with a female connector) and a male cap in order, and can be connected with a leak detection pressurizing pipeline for tightness detection.
The fourth pipeline 604 is connected with the first pipeline 601 and the second pipeline 602 through T-shaped tee joints. The fourth pipeline 604 is connected with the bottom of the buffer dropping funnel 701, and liquid is fed from the bottom, so that bubbles can be reduced, and damage to cells when the bubbles are broken can be avoided.
Wherein, two ends of a fifth pipeline 605 special for the peristaltic pump are connected with a pipeline 605-A and a pipeline 605-B through a connector, the pipeline 605-A is connected with a buffer dropping funnel 701, and the pipeline 605-B is connected with a pipe clamp, a 0.22 mu m hydrophobic gas filter (with a female connector) and a male cap in sequence. When the buffer drip chamber is used, the male cap is unscrewed, the peristaltic pump reversely pumps air, so that liquid in the cell liquid bag 101 and the buffer liquid bag 102 enters the buffer drip chamber 701, the peristaltic pump positively rotates to blow and pressurize, and the liquid entering the buffer drip chamber 701 is discharged and enters the subsequent pipeline 606.
The sixth pipeline 606 is connected with the bottom liquid outlet of the buffer dropping funnel 701 and supplies liquid for the subsequent pipeline, the sixth pipeline 606 is provided with the bubble sensor 401, when the bubble sensor detects that liquid exists in the pipeline, a feedback signal is sent to the control system, and the system controls the peristaltic pump to start metering, so that accurate quantitative liquid adding is provided for the subsequent pipeline.
The sixth pipeline 606 is connected with the seventh pipeline 607, the eighth pipeline 608 and the ninth pipeline 609 through cross type four-way joints.
The seventh pipeline 607, the eighth pipeline 608, and the ninth pipeline 609 are respectively provided with the third pinch valve 203, the fourth pinch valve 204, and the fifth pinch valve 205, and are respectively connected to the first cell sorting pipe 501, the second cell sorting pipe 502, and the third cell sorting pipe 503, and then respectively connected to the tenth pipeline 610, the eleventh pipeline 611, and the twelfth pipeline 612.
The tenth pipe 610, the eleventh pipe 611, and the twelfth pipe 612 are respectively provided with a sixth pinch valve 206, a seventh pinch valve 207, and an eighth pinch valve 208, and are connected to the thirteenth pipe 613 after being integrated by a cross-type four-way joint.
The thirteenth pipeline 613 is connected with the fourteenth pipeline 614 and the fifteenth pipeline 615 through T-shaped three-way connectors.
The fourteenth pipeline 614 and the fifteenth pipeline 615 are respectively provided with a ninth pinch valve 209 and a tenth pinch valve 210, so that female caps on the fourteenth pipeline 614 and the fifteenth pipeline 615 can be taken down in the biosafety cabinet, and the male connectors of the fourteenth pipeline 614 and the fifteenth pipeline 615 are in butt joint with the female connectors of the cell collection liquid bag 801 and the waste liquid bag 802 after the male caps are taken down, so that liquid draining is provided for a pipeline system.
Cell sorting tube design:
as shown in fig. 4, the first cell sorting tube 501, the second cell sorting tube 502 and the third cell sorting tube 503 are made of PC or PS and other transparent hard plastic materials meeting various biocompatibility, and the top of each sorting tube is provided with two interfaces. Wherein, a 0.22 mu m hydrophobic gas filter (with a female connector) and a male cap are sequentially arranged on the left interface; the male cap is required to be taken down before production and use, and is used for maintaining pressure balance in the sorting tube during liquid adding. The right side interface is a joint component 504, comprising a joint 5041 and a cap 5042, wherein the cap 5042 can be taken down in the biosafety cabinet, the magnetic beads are injected into the cell sorting tube through a syringe, and then the cap 5042 is installed, screwed and sealed on the joint 5041.
Pipeline system leak detection description:
before detection, the male caps on the left side interfaces of the first cell sorting tube 501, the second cell sorting tube 502 and the third cell sorting tube 503 are required to be installed and screwed; during detection, the male cap of the third pipeline 603 is unscrewed, a 0.22 mu m hydrophobic gas filter (with a female connector) is connected with a leak detection pressurizing pipeline, the first pinch valve 201, the second pinch valve 202, the ninth pinch valve 209 and the tenth pinch valve 210 are closed, the third pinch valve 203, the fourth pinch valve 204, the fifth pinch valve 205, the sixth pinch valve 206, the seventh pinch valve 207 and the eighth pinch valve 208 are opened, a peristaltic pump is closed, the pipe clamp of the third pipeline 603 is opened, and the pipeline is pressurized; stopping pressurizing when the pressure in the pipeline system reaches the detection limit value; if there is no exceeding pressure drop in the pipeline within the pressure set time, the tightness detection is qualified, after detection, the third pipeline 603 is disconnected from the leakage detection pressurizing pipeline, after the pipeline system is deflated, the pipe clamp is closed, and the male cap of the third pipeline 603 is screwed.
Description of the use case:
the sorting system can be used for sorting target cells in the cell sap after centrifugation and can also be used for removing magnetic beads in the cell sap after sorting and expanding culture.
The process for sorting target cells in the cell sap after centrifugation is as follows:
1) Adding magnetic beads into a first cell sorting pipe 501, a second cell sorting pipe 502 and a third cell sorting pipe 503, opening a second pinch valve 202, a third pinch valve 203, a fourth pinch valve 204 and a fifth pinch valve 205, pumping air by using a peristaltic pump to enable buffer liquid in a buffer liquid bag 102 to enter a buffer drip bucket 701, blowing air to pressurize and enter each sorting pipe to clean the magnetic beads, enabling a magnet to approach the sorting pipe, adsorbing the magnetic beads in each sorting pipe by using the magnet, opening a sixth pinch valve 206, a seventh pinch valve 207, an eighth pinch valve 208 and a tenth pinch valve 210 to discharge waste liquid into a waste liquid bag 802, closing all the pinch valves, and keeping the magnet away from the sorting pipe;
2) Opening a first pinch valve 201, a third pinch valve 203, a fourth pinch valve 204, a fifth pinch valve 205, a peristaltic pump 301 and a bubble sensor 401, pumping air by using the peristaltic pump 301 to enable cell liquid in a cell liquid bag 101 to enter a buffer drip hopper 701, blowing air and pressurizing the cell liquid into each sorting tube, vibrating and uniformly mixing cells and magnetic beads in the sorting tubes to enable target cells and the magnetic beads to be fully combined and incubated, enabling a magnet to be close to the sorting tubes, adsorbing a combination of the target cells and the magnetic beads in each sorting tube by using the magnet, opening a sixth pinch valve 206, a seventh pinch valve 207, an eighth pinch valve 208 and a tenth pinch valve 210 to discharge non-target cells and other impurities into a waste liquid bag 802, closing all the pinch valves, and keeping the magnet away from the sorting tubes;
3) Opening a second pinch valve 202, a third pinch valve 203, a fourth pinch valve 204, a fifth pinch valve 205, a peristaltic pump 301 and a bubble sensor 401, pumping air by using the peristaltic pump 301 to enable buffer liquid in a buffer liquid bag 102 to enter a buffer drip bucket 701, blowing air to pressurize and enter each sorting pipe, vibrating and washing cells in the sorting pipes, enabling a magnet to approach the sorting pipes, adsorbing magnetic beads in the sorting pipes by using the magnet, opening a sixth pinch valve 206, a seventh pinch valve 207, an eighth pinch valve 208 and a tenth pinch valve 210 to discharge non-target cells and other impurities into a waste liquid bag 802, closing all the pinch valves, and enabling the magnet to be far away from the sorting pipes;
4) Opening a second pinch valve 202, a third pinch valve 203, a fourth pinch valve 204, a fifth pinch valve 205, a peristaltic pump 301 and a bubble sensor 401, pumping air by using the peristaltic pump 301 to enable buffer liquid in the buffer liquid bag 102 to enter a buffer drip bucket 701, blowing air to pressurize and enter each sorting pipe, vibrating and resuspending cells in the sorting pipes, and opening a sixth pinch valve 206, a seventh pinch valve 207, an eighth pinch valve 208 and a ninth pinch valve 209 to discharge target cells to a cell collection liquid bag 801; and repeated once again.
When the system is used for removing the magnetic beads in the cell sap after the sorting and expanding culture, the process is as follows:
1) Opening a first pinch valve 201, a third pinch valve 203, a fourth pinch valve 204, a fifth pinch valve 205, a peristaltic pump 301 and a bubble sensor 401, pumping air by using the peristaltic pump 301 to enable cell liquid in the liquid bag 101 to enter a buffer drip hopper 701, blowing air to pressurize and enter each sorting pipe, vibrating and uniformly mixing liquid in the sorting pipes, enabling a magnet to be close to the sorting pipes, adsorbing a combination of cells and magnetic beads in the sorting pipes by using the magnet, opening a sixth pinch valve 206, a seventh pinch valve 207, an eighth pinch valve 208 and a ninth pinch valve 209 to discharge target cells without the magnetic beads to a cell collection liquid bag 801, closing all the pinch valves, and keeping the magnet away from the sorting pipes;
2) Repeating the step a for a plurality of times until the treatment of the cell fluid in the cell fluid bag 101 is completed;
3) Opening a second pinch valve 202, a third pinch valve 203, a fourth pinch valve 204, a fifth pinch valve 205, a peristaltic pump 301 and a bubble sensor 401, pumping air by using the peristaltic pump 301 to enable buffer liquid in a buffer liquid bag 102 to enter a buffer drip bucket 701, blowing air to pressurize and enter a sorting pipe, vibrating and washing cells in the sorting pipe, enabling a magnet to be close to the sorting pipe, adsorbing a combination of target cells and magnetic beads in the sorting pipe by using the magnet, opening a sixth pinch valve 206, a seventh pinch valve 207, an eighth pinch valve 208 and a ninth pinch valve 209 to discharge target cells without the magnetic beads to a cell collection liquid bag 801, closing all the pinch valves, and keeping the magnet away from the sorting pipe;
4) The second pinch valve 202, the third pinch valve 203, the fourth pinch valve 204, the fifth pinch valve 205, the peristaltic pump 301 and the bubble sensor 401 are opened, the peristaltic pump 301 is used for pumping air to enable buffer liquid in the liquid bag 102 to enter the buffer drip hopper 701, then air is blown and pressurized into the sorting pipe, the combination of cells and magnetic beads in the sorting pipe is subjected to vibration and resuspension, and the sixth pinch valve 206, the seventh pinch valve 207, the eighth pinch valve 208 and the tenth pinch valve 210 are opened to discharge the combination of the cells and the magnetic beads into the waste liquid bag 802.
Other description:
the number of the cell sorting pipes and the front and rear pinch valves in the sorting system can be adjusted according to the requirement of the production usage amount;
the sorting system performs cell sorting or cell demagnetization in a closed system environment, does not need to provide a GMP environment, and reduces the maintenance cost of the production environment;
the liquid bags and the pipelines of the sorting system can be directly connected to form a set of complete consumable materials for sterilization and packaging treatment; the liquid bag and the pipeline can be separated for sterilization and packaging treatment, and when in use, the liquid bag and the pipeline are connected in the biosafety cabinet through the male and female butt joints, and then the experiment or production is carried out.
The sorting system is a disposable product, sterile conditions are required to be ensured, ethylene oxide sterilization or gamma ray irradiation sterilization is carried out, and a medical grade sterilization packaging bag is adopted for packaging; the packaging bag is provided with a two-dimensional identification code, a code scanning gun is adopted to carry out code scanning input operation during use, and consumable information is recorded; after the use, the pipeline can be sealed by heat sealing, and then pollution-free treatment is carried out according to the medical waste standard, so that the leakage is prevented from polluting the environment.
The utility model forms a set of cell automatic sorting consumable materials by reasonably connecting and designing the liquid bag, the pipeline, the pinch valve, the peristaltic pump, the bubble sensor, the sorting pipe and the like, and reduces pollution risks caused by human intervention in the research, development and production process of cell therapeutic products.
The buffer dropping funnel is designed, the liquid in the buffer dropping funnel is controlled to flow in and discharge through the pumping and the inflating of the buffer dropping funnel, so that the pumping is not contacted with cells, the cell conveying can be finished, and the damage of the roller rolling in the pumping to the activity and the form of the cells is avoided. Wherein the cell inlet and outlet of the buffer dropping funnel are arranged at the bottom, and the pumping and blowing ports of the pump are arranged at the top, so that the generation of bubbles is reduced, and the damage to cells when the bubbles are broken is reduced.
The cylindrical cell sorting tube and the top interface thereof can be designed to rapidly add magnetic beads into a biosafety cabinet, and can also maintain pressure balance in the sorting tube during liquid adding.
The utility model can be used for separating target cells in the cell fluid after centrifugation and removing magnetic beads in the cell fluid after separation and expansion culture.
The utility model can avoid pollution risks caused by human intervention in the research and development and production processes of cell therapy products, ensure the repeatability consistency of cell sorting and demagnetizing bead results, and meet the requirements of regulations on disposable, sterile and airtight consumables for cell medicine production.
The above is only a preferred embodiment of the present utility model, and the protection scope of the present utility model is not limited to the above examples, and all technical solutions belonging to the concept of the present utility model belong to the protection scope of the present utility model. It should be noted that modifications and adaptations to the utility model without departing from the principles thereof are intended to be within the scope of the utility model as set forth in the following claims.
Claims (10)
1. The utility model provides a cell automation sorting system, its characterized in that includes flourishing liquid unit (1), buffer vessel (7), air pump unit (3), cell sorting unit (5) and receipts liquid unit (8), flourishing liquid unit (1) with the inlet of buffer vessel (7) links to each other, the liquid outlet of buffer vessel (7) warp cell sorting unit (5) with receive liquid unit (8) links to each other, air pump unit (3) with buffer vessel (7) intercommunication for bleed or aerify buffer vessel (7) and realize inflow and the discharge of liquid in buffer vessel (7).
2. The automated cell sorting system according to claim 1, wherein the liquid inlet and the liquid outlet of the buffer container (7) are both located at the bottom of the buffer container (7), and the air pump unit (3) is in communication with the top of the buffer container (7).
3. The automated cell sorting system of claim 1, wherein the buffer container (7) is a drip funnel (701).
4. A cell automated sorting system according to claim 1, 2 or 3, wherein the cell sorting unit (5) comprises a plurality of cell sorting pipes (501), the inlets of the plurality of cell sorting pipes (501) are each connected to the liquid outlet of the buffer container (7), and the outlets of the plurality of cell sorting pipes (501) are each connected to the liquid receiving unit (8).
5. The automated cell sorting system of claim 4, wherein each cell sorting tube (501) is provided with a connector assembly (504) for injecting magnetic beads.
6. The automated cell sorting system of claim 5, wherein the connector assembly (504) comprises a connector (5041) and a cap (5042), the connector (5041) is located at the top of the cell sorting tube (501), and the cap (5042) is covered on the connector (5041).
7. A cell automated sorting system according to claim 1 or 2 or 3, characterized in that the air pump unit (3) is a peristaltic pump (301).
8. An automated cell sorting system according to claim 1 or 2 or 3, characterized in that a bubble sensor (401) is provided on the line between the buffer container (7) and the cell sorting unit (5).
9. The automated cell sorting system according to claim 1, 2 or 3, further comprising a tightness detection unit (9) for detecting tightness of a pipeline between a liquid outlet of the liquid containing unit (1) and a liquid inlet of the liquid receiving unit (8).
10. The automated cell sorting system according to claim 9, wherein the tightness detection unit (9) comprises a pressurizing assembly and a pressure detection assembly, the pressurizing assembly being used for charging pressurized gas into a pipeline between a liquid outlet of the liquid containing unit (1) and a liquid inlet of the liquid receiving unit (8); the pressure detection component is used for detecting the pressure of a pipeline between a liquid outlet of the liquid containing unit (1) and a liquid inlet of the liquid receiving unit (8) in a preset time period after pressurization.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202223602318.6U CN219385102U (en) | 2022-12-27 | 2022-12-27 | Automatic cell sorting system |
| PCT/CN2023/093281 WO2024139004A1 (en) | 2022-12-27 | 2023-05-10 | Automatic cell sorting system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202223602318.6U CN219385102U (en) | 2022-12-27 | 2022-12-27 | Automatic cell sorting system |
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| CN219385102U true CN219385102U (en) | 2023-07-21 |
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| Country | Link |
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| CN (1) | CN219385102U (en) |
| WO (1) | WO2024139004A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117089458A (en) * | 2023-10-19 | 2023-11-21 | 南京溯远基因科技有限公司 | Device and method for automatic cell culture, gene editing, cell sorting and split charging |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205538983U (en) * | 2015-12-31 | 2016-08-31 | 上海白泽医疗器械有限公司 | A device, cellular immunity magnetic bead sorting unit and cover box for cellular immunity magnetic bead is selected separately |
| CN110951579A (en) * | 2019-08-09 | 2020-04-03 | 上海乘黄纳米抗体科技有限公司 | Immunomagnetic bead sorting container, sorting device and sorting system |
| CN112080393B (en) * | 2020-09-29 | 2021-09-10 | 深圳睿思生命科技有限公司 | Automatic separation and enrichment device and method for tumor cells |
| CN112094839B (en) * | 2020-11-06 | 2021-03-16 | 深圳市赛特罗生物医疗技术有限公司 | Automatic cell magnetic sorting method and device |
| CN116445276A (en) * | 2023-04-12 | 2023-07-18 | 楚天思为康基因科技(长沙)有限公司 | Disposable cell sorting device and cell sorting device |
-
2022
- 2022-12-27 CN CN202223602318.6U patent/CN219385102U/en active Active
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117089458A (en) * | 2023-10-19 | 2023-11-21 | 南京溯远基因科技有限公司 | Device and method for automatic cell culture, gene editing, cell sorting and split charging |
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| WO2024139004A1 (en) | 2024-07-04 |
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