CN219930125U - Disposable cell sorting device and cell sorting device - Google Patents
Disposable cell sorting device and cell sorting device Download PDFInfo
- Publication number
- CN219930125U CN219930125U CN202320814282.3U CN202320814282U CN219930125U CN 219930125 U CN219930125 U CN 219930125U CN 202320814282 U CN202320814282 U CN 202320814282U CN 219930125 U CN219930125 U CN 219930125U
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- Prior art keywords
- container
- cell sorting
- sorting apparatus
- disposable cell
- disposable
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- 238000012546 transfer Methods 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000009423 ventilation Methods 0.000 claims abstract description 22
- 210000001503 joint Anatomy 0.000 claims abstract description 9
- 239000011324 bead Substances 0.000 claims description 15
- 239000000872 buffer Substances 0.000 claims description 11
- 239000002699 waste material Substances 0.000 claims description 11
- 238000005070 sampling Methods 0.000 claims description 6
- 239000000945 filler Substances 0.000 claims description 5
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 9
- 230000008569 process Effects 0.000 abstract description 9
- 238000009434 installation Methods 0.000 abstract description 6
- 230000005779 cell damage Effects 0.000 abstract description 5
- 208000037887 cell injury Diseases 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 230000007246 mechanism Effects 0.000 description 8
- 230000009471 action Effects 0.000 description 6
- 230000002572 peristaltic effect Effects 0.000 description 6
- 238000003032 molecular docking Methods 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 238000005273 aeration Methods 0.000 description 2
- 238000002659 cell therapy Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012943 hotmelt Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Abstract
The utility model discloses a disposable cell sorting device which comprises a transfer container, a rotary joint and a sorting container connected with the rotary joint, wherein the transfer container is communicated with the sorting container through a pipeline, the transfer container is provided with a ventilation connecting part for enabling the inside of the transfer container to be ventilated to generate positive pressure or negative pressure, and the pipeline is provided with a first butt joint part for connecting an initial container, a second butt joint part for connecting a sorting liquid container and a third butt joint part for connecting a collecting container. The utility model also discloses a cell sorting unit, including mounting bracket and foretell disposable cell sorting utensil, be equipped with installation department, a plurality of pinch valve and a plurality of pipe fitting admittedly on the mounting bracket, transfer container demountable installation is on the installation department, and the pipeline is fixed on admittedly the pipe fitting, and the pinch valve presss from both sides to locate on the corresponding pipe position. The disposable cell sorting device and the cell sorting device have the advantages of avoiding cell damage in the feeding and conveying processes, greatly improving the cell yield and the cell activity and the like.
Description
Technical Field
The utility model relates to the technical field of cell medicine preparation, in particular to a disposable cell sorting device and a cell sorting device.
Background
At present, the cell therapy technology at home and abroad is developed rapidly, and cell therapy products are directly input into human bodies and cannot be subjected to terminal sterilization or other virus removal process treatment. In laboratories, research and development institutions and related production, common cells are centrifuged and then are directly separated in a magnetic field, other manual operations are performed, pollution to cell samples is easy to occur, and the yield and activity of target cells of the existing automatic separation equipment in the market are not high.
The applicant has filed patent applications (such as 202221782866.9) related to a cell sorting device to realize automatic sorting of cells to avoid pollution to cell samples caused by manual operation, but products corresponding to the patent applications find that certain cracks exist in the sorted cells in the testing and using processes. Further intensive studies have found that the reason for this is that the pump directly pumps and discharges the cell fluid, which in turn causes cell disruption, causes cell damage, and reduces cell yield and viability.
Disclosure of Invention
The utility model aims to solve the technical problem of overcoming the defects of the prior art and providing a disposable cell sorting device and a cell sorting device which can avoid cell damage in the feeding and conveying process and greatly improve the cell yield and the cell activity.
In order to solve the technical problems, the utility model adopts the following technical scheme:
the utility model provides a disposable cell sorting utensil, includes transfer container, rotary joint and the sorting container of being connected with rotary joint, transfer container passes through the pipeline intercommunication with the sorting container, transfer container is equipped with the aeration connecting portion that makes its inside aeration produce malleation or negative pressure, the pipeline is equipped with the first interfacing part that is used for connecting initial container, is used for connecting the second interfacing part of sorting liquid container and is used for connecting the third interfacing part of collecting container.
Disposable cell sorting device and cell sorting apparatus.
In order to solve the technical problems, the utility model adopts the following technical scheme:
the utility model provides a disposable cell sorting utensil, includes transfer container, sorting container and the pipeline of intercommunication between transfer container and sorting container, the transfer container is equipped with the ventilation connecting portion that makes its inside ventilation produce malleation or negative pressure, the pipeline is equipped with the butt joint portion that is used for connecting initial container, the sorting container is equipped with magnetic bead filler opening and bin outlet.
As a further improvement of the above technical scheme:
the docking portion docks with an initial container.
The pipeline is provided with a first connecting part for connecting with the buffer solution container.
The first connecting part is connected with a buffer container.
The discharge opening is connected with a liquid discharge pipe, and the liquid discharge pipe is provided with a second connecting part for connecting the collecting container.
The second connecting part is connected with a collecting container.
The collection container is provided with a sampling interface and/or a first gas filter.
The liquid discharge pipe is provided with a third connecting part for connecting the waste liquid container.
The third connecting part is connected with a waste liquid container.
The pipe is provided with a fourth connection for leak detection.
The pipeline is connected with the bottom of the transfer container.
The ventilation connecting part is arranged on the side wall or the top wall of the transfer container.
The ventilation connecting part is connected with a positive and negative pressure device.
The positive and negative pressure device comprises a second gas filter and a gas pipe, and the second gas filter is communicated with the transfer container through the gas pipe.
The transfer container is a hard container.
The sorting container is provided with a breathing ventilation port.
The utility model provides a cell sorting unit, includes mounting bracket and foretell disposable cell sorting utensil, be equipped with installation department, a plurality of pinch valve and a plurality of solid pipe fitting on the mounting bracket, transfer container demountable installation is on the installation department, the pipeline is fixed on solid pipe fitting, the pinch valve presss from both sides to locate on corresponding pipe position.
Compared with the prior art, the utility model has the advantages that:
when the disposable cell sorting device is used, an initial container containing cell liquid is connected with a butt joint part in a sterile environment, and a sorting container is arranged beside a magnetic attraction mechanism; then firstly making the cell liquid in the initial container enter the transfer container under the action of the negative pressure (formed by air suction of the positive and negative pressure devices) of the transfer container, and then making the cell liquid in the transfer container enter the sorting container under the action of the positive pressure (formed by air inflation of the positive and negative pressure devices) of the transfer container to be combined with the magnetic beads (through the magnetic bead filling opening Wang Ru) in the sorting container; the magnetic attraction mechanism applies larger magnetic field intensity to the sorting container, the magnetic beads and the cell combination in the sorting container are adsorbed on one side of the sorting container close to the magnetic attraction mechanism, and then liquid in the sorting container is discharged through the discharge port, so that the magnetic beads and the cell combination are left; then, cells in the magnetic bead-cell combination are sorted by repeatedly injecting a buffer solution into the sorting container, changing the magnetic field intensity of the sorting container by the magnetic attraction mechanism, and discharging the supernatant. Therefore, the material (cell sap) does not directly pass through the positive and negative pressure device in the feeding process, is not subjected to repeated extrusion action of the positive and negative pressure device, avoids cell damage in the feeding and conveying process, and greatly improves the cell yield and the cell activity.
According to the cell sorting device, the disposable cell sorting device is detachably arranged on the mounting frame, and scrapped after the disposable cell sorting device is used. The sorting container, the initial container, the buffer container and other containers are arranged in the sorting device or on the hooks. Therefore, the pinch valve is not disposable with the disposable cell sorting device, can be used for multiple times, and saves cost.
Drawings
FIG. 1 is a schematic diagram of the structure of a disposable cell sorter of the present utility model.
FIG. 2 is a schematic view showing the structure of a collection container of the disposable cell sorter of the present utility model.
FIG. 3 is a diagram of the cooperation of the disposable cell sorting apparatus of the present utility model with a peristaltic pump.
Fig. 4 is an enlarged view of fig. 3 a.
FIG. 5 is a packaging view of the disposable cell sorter of the present utility model.
FIG. 6 is a schematic structural view of a packing bag of the disposable cell sorter of the present utility model.
FIG. 7 is a side view of the cell sorter of the present utility model.
The reference numerals in the drawings denote:
1. a transfer container; 10. a pipeline; 101. a fourth connecting portion; 11. a ventilation connection; 12. packaging bags; 13. a peristaltic pump; 2. a sorting container; 21. a magnetic bead filler; 22. a discharge port; 221. a liquid discharge pipe; 23. a breathing ventilation port; 3. an initial container; 31. a butt joint part; 4. a buffer container; 41. a first connection portion; 5. a collection container; 51. a second connecting portion; 52. a sampling interface; 53. a first gas filter; 6. a waste liquid container; 61. a third connecting portion; 7. positive and negative pressure devices; 70. an air pipe; 71. a second gas filter; 8. a mounting frame; 81. a mounting part; 82. a pinch valve; 83. and fixing the pipe fitting.
Detailed Description
The utility model will be described in further detail with reference to the drawings and the specific examples.
Embodiment one:
fig. 1 to 6 show an embodiment of the disposable cell sorting apparatus of the present utility model, which comprises a relay container 1, a sorting container 2, and a pipe 10 communicating between the relay container 1 and the sorting container 2, the relay container 1 being provided with a ventilation connection portion 11 for generating positive or negative pressure for ventilation of the inside thereof, the pipe 10 being provided with a docking portion 31 for connection with an initial container 3, the sorting container 2 being provided with a bead filler port 21 and a discharge port 22.
When in use, the initial container 3 containing the cell liquid is connected with the butt joint part 31 in a sterile environment, and the sorting container 2 is arranged beside the magnetic attraction mechanism; then firstly, making the cell liquid in the initial container 3 enter the transfer container 1 under the action of the negative pressure (formed by air suction of the positive and negative pressure device 7) of the transfer container 1, and then making the cell liquid in the transfer container 1 enter the sorting container 2 under the action of the positive pressure (formed by air inflation of the positive and negative pressure device 7) of the transfer container 1 to be combined with the magnetic beads (which are filled through the magnetic bead filling port 21) in the sorting container 2; the magnetic attraction mechanism applies larger magnetic field intensity to the sorting container 2, the magnetic bead and cell combination in the sorting container 2 is adsorbed on one side of the sorting container 2 close to the magnetic attraction mechanism, and then the liquid in the sorting container 2 is discharged through the discharge port 22, namely the magnetic bead and cell combination is left; then, cells in the magnetic bead-cell combination are sorted by repeatedly injecting a buffer solution into the sorting container 2, changing the magnetic field intensity of the sorting container 2 by the magnetic attraction mechanism, and discharging the supernatant. In this way, the material (cell sap) does not directly pass through the positive and negative pressure device 7 in the feeding process, is not subjected to repeated extrusion action of the positive and negative pressure device 7, avoids cell damage in the feeding and conveying process, and greatly improves the cell yield and the cell activity.
In this embodiment, the docking portion 31 is docked with the initial container 3. In this way, it is not necessary to prepare the initial container 3 which can be docked with the docking portion 31 at the time of subsequent use.
In this embodiment, the line 10 is provided with a first connection 41 for connecting to the buffer container 4. In use, the buffer container 4 is connected to the first connection 41 for supplying buffer to the sorting container 2.
In this embodiment, the buffer container 4 is connected to the first connection portion 41. In this way, it is not necessary to prepare the buffer container 4 which can be docked with the first connection portion 41 at the time of subsequent use.
In the present embodiment, the drain port 22 is connected to a drain pipe 221, and the drain pipe 221 is provided with a second connection portion 51 for connecting the collection container 5. In use, the collection container 5 is connected to the second connection portion 51, and the cells in the magnetic beads and cell combinations sorted in the sorting container 2 are collected by entering the collection container 5 through the drain 221.
In the present embodiment, the second connection portion 51 is connected to the collection container 5. In this way, there is no need to prepare a collection container 5 which can be docked with the second connection 51 at a later time.
In this embodiment, the collection container 5 is provided with a sampling interface 52 and/or a first gas filter 53. Sampling detection is facilitated by the provision of sampling interface 52. By providing a first gas filter 53 for balancing the gas pressure in the collection vessel 5.
In this embodiment, the drain pipe 221 is provided with a third connection portion 61 for connecting the waste liquid container 6. In use, the waste liquid container 6 is connected to the third connection portion 61, and the waste liquid in the sorting container 2 enters the waste liquid container 6 through the liquid discharge pipe 221 to be collected.
In this embodiment, the waste liquid container 6 is connected to the third connection portion 61. In this way, at the time of subsequent use, it is not necessary to prepare the waste liquid container 6 which can be docked with the third connecting portion 61.
In this embodiment, the line 10 is provided with a fourth connection 101 for leak detection. The fourth connection part 101 can detect whether the pipeline 10 leaks or not and the sealing condition of the pipeline 10, and the fourth connection part 101 is generally used for completing the test before the disposable sorting apparatus is assembled and shipped.
In this embodiment, the pipeline 10 is connected to the bottom of the transfer vessel 1.
In this embodiment, the ventilation connection 11 is provided on a side wall or a top wall of the transfer container 1. Thus, during the positive pressure process, gas enters from the side wall or the top wall of the transfer container 1, for example, the ventilation connection part 11 is arranged at the bottom of the transfer container 1, and when the transfer container 1 is filled with liquid, the liquid can enter the positive-negative pressure device 7 and even leak through the ventilation connection part 11.
In the present embodiment, the positive/negative pressure device 7 is connected to the ventilation connection portion 11. The positive-negative pressure device 7 includes a second gas filter 71 and a gas pipe 70, and the second gas filter 71 communicates with the relay tank 1 through the gas pipe 70. When in use, the air pipe 70 is arranged in the peristaltic pump 13 (as shown in fig. 3 and 4), the peristaltic pump 13 can be ventilated or pumped to the transfer container 1 through the air pipe 70 when in operation, so that positive pressure or negative pressure in the transfer container 1 is realized, the second air filter 71 is used for filtering impurities in air to prevent internal pollution of the culture appliance, and in addition, particles possibly occur when the peristaltic pump 13 clamps the air pipe 70 when in operation, the second air filter 71 can be arranged between the peristaltic pump 13 and the ventilation connecting part 11.
In this embodiment, the transfer container 1 is a rigid container. In this way, the relay container 1 is not deformed in the positive and negative pressure state.
In this embodiment, the sorting container 2 is provided with a breathing ventilation opening 23. The breathing ventilation port 23 is used to equalize the pressure inside the sorting container 2 when charging. The bead filler 21 is used to add beads to the sorting vessel 2 prior to sorting.
In this embodiment, as shown in FIGS. 5 and 6, the disposable cell culture apparatus further comprises a packing bag 12, and each container and the tube 10 are packed in the packing bag 12.
In this embodiment, the pipe 10 and the container may be connected by a Luer (Luer) or a two-way connector, that is, the connection portion is a Luer (Luer) or a two-way connector, or the pipe 10 and the container may be connected by a connection device, for example, a hot-melt welding machine.
Embodiment two:
fig. 7 shows an embodiment of the cell sorting apparatus according to the present utility model, which comprises a mounting frame 8 and a disposable cell sorting device for implementing one, wherein the mounting frame 8 is provided with a mounting portion 81, a plurality of pinch valves 82 and a plurality of fixing pipes 83, the transfer container 1 is detachably mounted on the mounting portion 81, the pipeline 10 is fixed on the fixing pipes 83, and the pinch valves 82 are clamped on the corresponding pipe positions. The disposable cell sorting device is detachably arranged on the mounting frame 8, and is scrapped after being used. The sorting container 2, the initial container 3, the buffer container 4, and the like are provided in the sorting apparatus or on the hook. In this way, pinch valve 82 is not disposable with a disposable cell sorting apparatus, can be used multiple times, and saves costs.
While the utility model has been described in terms of preferred embodiments, it is not intended to be limiting. Many possible variations and modifications of the disclosed technology can be made by anyone skilled in the art, or equivalent embodiments with equivalent variations can be made, without departing from the scope of the utility model. Therefore, any simple modification, equivalent variation and modification of the above embodiments according to the technical substance of the present utility model shall fall within the scope of the technical solution of the present utility model.
Claims (17)
1. A disposable cell sorting apparatus, characterized in that: including transfer container (1), sorting container (2) and intercommunication pipeline (10) between transfer container (1) and sorting container (2), transfer container (1) are equipped with and make its inside ventilation produce ventilation connecting portion (11) of malleation or negative pressure, pipeline (10) are equipped with butt joint portion (31) that are used for connecting initial container (3), sorting container (2) are equipped with magnetic bead filler (21) and bin outlet (22).
2. The disposable cell sorting apparatus according to claim 1, wherein: the butt joint part (31) is butt-jointed with an initial container (3).
3. The disposable cell sorting apparatus according to claim 1, wherein: the line (10) is provided with a first connection (41) for connecting a buffer container (4).
4. The disposable cell sorting apparatus according to claim 3, wherein: the first connection part (41) is connected with a buffer container (4).
5. The disposable cell sorting apparatus according to claim 1, wherein: the discharge port (22) is connected with a liquid discharge pipe (221), and the liquid discharge pipe (221) is provided with a second connecting part (51) for connecting the collecting container (5).
6. The disposable cell sorting apparatus according to claim 5, wherein: the second connecting part (51) is connected with a collecting container (5).
7. The disposable cell sorting apparatus according to claim 6, wherein: the collection container (5) is provided with a sampling interface (52) and/or a first gas filter (53).
8. The disposable cell sorting apparatus according to claim 5, wherein: the liquid discharge pipe (221) is provided with a third connecting part (61) for connecting the waste liquid container (6).
9. The disposable cell sorting apparatus according to claim 8, wherein: the third connecting part (61) is connected with a waste liquid container (6).
10. The disposable cell sorting apparatus according to claim 1, wherein: the line (10) is provided with a fourth connection (101) for leak detection.
11. The disposable cell sorting apparatus according to claim 1, wherein: the pipeline (10) is connected with the bottom of the transfer container (1).
12. The disposable cell sorting apparatus according to any one of claims 1 to 11, characterized in that: the ventilation connecting part (11) is arranged on the side wall or the top wall of the transfer container (1).
13. The disposable cell sorting apparatus according to any one of claims 1 to 11, characterized in that: the ventilation connecting part (11) is connected with a positive and negative pressure device (7).
14. The disposable cell sorting apparatus of claim 13, wherein: the positive and negative pressure device (7) comprises a second gas filter (71) and a gas pipe (70), and the second gas filter (71) is communicated with the transfer container (1) through the gas pipe (70).
15. The disposable cell sorting apparatus according to any one of claims 1 to 11, characterized in that: the transfer container (1) is a hard container.
16. The disposable cell sorting apparatus according to any one of claims 1 to 11, characterized in that: the sorting container (2) is provided with a breathing ventilation opening (23).
17. A cell sorting apparatus, characterized in that: the disposable cell sorting apparatus comprises a mounting frame (8) and any one of claims 1 to 16, wherein a mounting portion (81), a plurality of pinch valves (82) and a plurality of fixed pipe fittings (83) are arranged on the mounting frame (8), the transfer container (1) is detachably arranged on the mounting portion (81), the pipeline (10) is fixed on the fixed pipe fittings (83), and the pinch valves (82) are clamped on corresponding pipe positions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320814282.3U CN219930125U (en) | 2023-04-12 | 2023-04-12 | Disposable cell sorting device and cell sorting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320814282.3U CN219930125U (en) | 2023-04-12 | 2023-04-12 | Disposable cell sorting device and cell sorting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219930125U true CN219930125U (en) | 2023-10-31 |
Family
ID=88498414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320814282.3U Active CN219930125U (en) | 2023-04-12 | 2023-04-12 | Disposable cell sorting device and cell sorting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219930125U (en) |
-
2023
- 2023-04-12 CN CN202320814282.3U patent/CN219930125U/en active Active
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