CN212688085U - Cell culture instrument - Google Patents
Cell culture instrument Download PDFInfo
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- CN212688085U CN212688085U CN202020612263.9U CN202020612263U CN212688085U CN 212688085 U CN212688085 U CN 212688085U CN 202020612263 U CN202020612263 U CN 202020612263U CN 212688085 U CN212688085 U CN 212688085U
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- cell culture
- reagent
- rubber tubes
- centrifugal chamber
- machine
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Abstract
The utility model discloses a cell culture instrument, including reagent stores pylon and work frame, the fixed a plurality of reagent couples that are provided with on the reagent stores pylon, it is equipped with a plurality of reagent bags to hang on a plurality of reagent couples, the fixed many rubber tubes and a plurality of pinch valves, peristaltic pump, centrifugal chamber, industry control all-in-one and waste liquid collection bag that are provided with on the work frame, a plurality of reagent bags are connected with peristaltic pump, centrifugal chamber and waste liquid collection bag intercommunication respectively through many rubber tubes, and also through connection between many rubber tubes, a plurality of pinch valves set up on the body of pipe of many rubber tubes respectively. The utility model discloses a cell culture instrument, the device can realize aseptic airtight operation, and no infection risk wholly once installs the back simultaneously, realizes all process flows of integrative control by special industrial control system, ensures the automation of cell culture overall process, has improved cell culture's efficiency and quality.
Description
Technical Field
The utility model relates to a cultivate the instrument field, especially relate to a cell culture instrument.
Background
Cell culture refers to a technique of simulating in vivo growth environment of cells collected from in vivo tissues, placing the cells under sterile conditions, proper temperature and pH value to grow and propagate the cells and maintain the structure and function of the cells, and has become a widely adopted technical method in biological and medical research and application.
The existing cell culture instrument needs to carry out sorting, enrichment and culture on different equipment in stages and steps, has high requirements on the quality of cell culture personnel, low controllability of the culture process, unstable culture quality and quantity and low automation degree.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a cell culture instrument.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a cell culture instrument, includes reagent stores pylon and work frame, the fixed a plurality of reagent couples that are provided with on the reagent stores pylon, hang on a plurality of reagent couples and be equipped with a plurality of reagent bags, the fixed many rubber tubes and a plurality of pinch valves, peristaltic pump, centrifugal chamber, industry control all-in-one and waste liquid collection bag that is provided with on the work frame, a plurality of reagent bags are connected with peristaltic pump, centrifugal chamber and waste liquid collection bag intercommunication respectively through many rubber tubes, and also through connection between many rubber tubes, a plurality of pinch valves set up respectively on the pipe shaft of many rubber tubes, a piping system is constituteed to a plurality of reagent bags and many rubber tubes, the fixed platform of placing in work frame one side upper end, it is fixed to be provided with heat sealing machine and sweeps a yard machine to place bench.
As a further description of the above technical solution:
a plurality of liquid level sensors are arranged in the pipeline system.
As a further description of the above technical solution:
the lower end of the centrifugal chamber is provided with a microscope.
As a further description of the above technical solution:
sweep ink recorder, heat-sealing machine, peristaltic pump, microscope all with industry control all-in-one electric connection.
As a further description of the above technical solution:
and the rear end of the industrial control all-in-one machine is provided with a magnetic bead, and the magnetic bead is sleeved outside one rubber pipe in the pipeline system.
As a further description of the above technical solution:
the centrifugal chamber and the pipe system form a closed loop.
The utility model discloses following beneficial effect has:
compared with the prior art, the cell culture instrument integrates the pipeline systems required by cell culture, integrates the necessary passages of the integrated pipeline systems for cell sorting, cell rinsing, cell transduction reagent adding, cell enrichment and cell culture process flow, controls the opening and closing of each pipeline by using a pinch valve, conveys cells and culture solution by a peristaltic pump, and completes the realization of each process flow step.
Compared with the prior device, the pipeline system is integrated into a whole, only needs to be integrally installed once, does not need to be disassembled and assembled for many times, completely realizes sterile closed operation, and has no infection risk; meanwhile, after the whole cell culture system is integrally installed once, all process flows are integrally controlled by a special industrial control system, the automation of the whole cell culture process is ensured, and the efficiency and the quality of cell culture are improved.
Drawings
FIG. 1 is a schematic structural diagram of a cell culture apparatus according to the present invention.
Illustration of the drawings:
1. a reagent hanger; 2. a working frame; 3. a piping system; 4. a reagent bag; 5. a rubber tube; 6. a pinch valve; 7. a placing table; 8. a heat sealing machine; 9. a code scanning machine; 10. a waste liquid collection bag; 11. a peristaltic pump; 12. A centrifugal chamber; 13. a liquid level sensor; 14. magnetic beads; 15. a microscope; 16. reagent hooks; 17. an industrial control all-in-one machine.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1, the present invention provides an embodiment: a cell culture instrument comprises a reagent hanging frame 1 and a working frame 2, a plurality of reagent hanging hooks 16 are fixedly arranged on the reagent hanging frame 1, a plurality of reagent bags 4 are hung on the reagent hanging hooks 16, nutrient media and culture media for cell culture are stored in the reagent bags 4, a plurality of rubber tubes 5, a plurality of pinch valves 6, a peristaltic pump 11, a centrifugal chamber 12, an industrial control all-in-one machine 17 and a waste liquid collecting bag 10 are fixedly arranged on the working frame 2, the reagent bags 4 are respectively communicated and connected with the peristaltic pump 11, the centrifugal chamber 12 and the waste liquid collecting bag 10 through the rubber tubes 5, the rubber tubes 5 are also communicated and connected, the pinch valves 6 are respectively arranged on tube bodies of the rubber tubes 5, the reagent bags 4 and the rubber tubes 5 form a pipeline system 3, the integrated pipeline system 3 sorts cells, rinses the cells, adds cell transduction reagents, necessary passages of the cell enrichment and cell culture process flow are integrated, each pipeline is controlled to be opened and closed by a pinch valve 6, cells and culture solution are conveyed by a peristaltic pump 11, the process flow steps are achieved, a placing table 7 is fixedly arranged at the upper end of one side of the working frame 2, and a heat sealing machine 8 and a code scanning machine 9 are fixedly arranged on the placing table 7.
Be provided with a plurality of level sensor 13 in pipe-line system 3, centrifugal chamber 12 lower extreme is provided with microscope 15, sweep ink recorder 9, heat-sealing machine 8, peristaltic pump 11, microscope 15 all with industry control all-in-one 17 electric connection, microscope 15 is used for the observation of cell growth condition, can carry out the state of microscopic level inspection cell culture to each time point of cell culture on industry control all-in-one 17 in the overall process of cell culture, grasp the quality of cell culture through process control, industry control all-in-one 17 rear end is provided with magnetic bead 14, magnetic bead 14 cover is established outside a rubber tube 5 in pipe-line system 3, centrifugal chamber 12 forms a closed circuit with pipe-line system 3.
The working principle is as follows: when the device is used, the whole device is installed, the whole cell culture process is automatically controlled through the industrial control all-in-one machine 17, the industrial control all-in-one machine 17 controls the cells in the centrifugal chamber 12 to be supplied with nutrition through the peristaltic pump 11 and the pipeline system 3, the pipeline system 3 and the centrifugal chamber 12 form a closed loop, a series of process flows such as cell sorting, cell rinsing, cell transduction reagent adding, cell enrichment and cell culture can be carried out, the industrial control all-in-one machine 17 can also carry out microscopic-level photographing on each time point of the cell culture through the microscope 15 to check the state of the cell culture, and the cell culture quality can be mastered through process control.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (6)
1. A cell culture instrument, includes reagent stores pylon (1) and work frame (2), its characterized in that: the reagent hanging rack (1) is fixedly provided with a plurality of reagent hooks (16), the plurality of reagent hooks (16) are hung with a plurality of reagent bags (4), the working rack (2) is fixedly provided with a plurality of rubber tubes (5), a plurality of pinch valves (6), a peristaltic pump (11), a centrifugal chamber (12), an industrial control all-in-one machine (17) and a waste liquid collecting bag (10), the plurality of reagent bags (4) are respectively communicated and connected with the peristaltic pump (11), the centrifugal chamber (12) and the waste liquid collecting bag (10) through a plurality of rubber tubes (5), the plurality of rubber tubes (5) are also communicated and connected, the plurality of pinch valves (6) are respectively arranged on tube bodies of the plurality of rubber tubes (5), the plurality of reagent bags (4) and the plurality of rubber tubes (5) form a pipeline system (3), a placing table (7) is fixedly arranged at the upper end of one side of the working rack (2), the placing table (7) is fixedly provided with a heat sealing machine (8) and a code scanning machine (9).
2. A cell culture apparatus according to claim 1, wherein: a plurality of liquid level sensors (13) are arranged in the pipeline system (3).
3. A cell culture apparatus according to claim 1, wherein: the lower end of the centrifugal chamber (12) is provided with a microscope (15).
4. A cell culture apparatus according to claim 1, wherein: the code scanning machine (9), the heat sealing machine (8), the peristaltic pump (11) and the microscope (15) are all electrically connected with the industrial control all-in-one machine (17).
5. A cell culture apparatus according to claim 1, wherein: the rear end of the industrial control integrated machine (17) is provided with a magnetic bead (14), and the magnetic bead (14) is sleeved outside a rubber tube (5) in the pipeline system (3).
6. A cell culture apparatus according to claim 1, wherein: the centrifugal chamber (12) and the pipe system (3) form a closed loop.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020612263.9U CN212688085U (en) | 2020-04-20 | 2020-04-20 | Cell culture instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020612263.9U CN212688085U (en) | 2020-04-20 | 2020-04-20 | Cell culture instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212688085U true CN212688085U (en) | 2021-03-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020612263.9U Active CN212688085U (en) | 2020-04-20 | 2020-04-20 | Cell culture instrument |
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| CN (1) | CN212688085U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117089458A (en) * | 2023-10-19 | 2023-11-21 | 南京溯远基因科技有限公司 | Device and method for automatic cell culture, gene editing, cell sorting and split charging |
-
2020
- 2020-04-20 CN CN202020612263.9U patent/CN212688085U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117089458A (en) * | 2023-10-19 | 2023-11-21 | 南京溯远基因科技有限公司 | Device and method for automatic cell culture, gene editing, cell sorting and split charging |
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| TR01 | Transfer of patent right |
Effective date of registration: 20211101 Address after: 215222 2F, complex building, pangjin industrial building, No. 1801, pangjin Road, Jiangling street, Wujiang District, Suzhou City, Jiangsu Province Patentee after: Suzhou Xinxie Biotechnology Co.,Ltd. Address before: Room 589-107, building 2, Pudong New Area, Shanghai Patentee before: Shanghai Xinxie Biotechnology Co.,Ltd. |