CN211871958U - Soil aeration culture device - Google Patents
Soil aeration culture device Download PDFInfo
- Publication number
- CN211871958U CN211871958U CN202020191338.0U CN202020191338U CN211871958U CN 211871958 U CN211871958 U CN 211871958U CN 202020191338 U CN202020191338 U CN 202020191338U CN 211871958 U CN211871958 U CN 211871958U
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- CN
- China
- Prior art keywords
- bottle
- soil
- culture device
- intake pipe
- soil aeration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000002689 soil Substances 0.000 title claims abstract description 40
- 238000005273 aeration Methods 0.000 title claims description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 241000233866 Fungi Species 0.000 claims abstract description 9
- 229920000742 Cotton Polymers 0.000 claims abstract description 7
- 210000003437 trachea Anatomy 0.000 claims abstract description 4
- 229910052751 metal Inorganic materials 0.000 claims description 10
- 239000002184 metal Substances 0.000 claims description 10
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 239000000654 additive Substances 0.000 abstract description 8
- 230000000996 additive effect Effects 0.000 abstract description 7
- 230000008859 change Effects 0.000 abstract description 5
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 238000004088 simulation Methods 0.000 abstract description 2
- 238000009423 ventilation Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000006740 morphological transformation Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000003516 soil conditioner Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a soil culture apparatus that ventilates, including bottle, bottle lid, the bottle lid covers the bottleneck of bottle, intake pipe, outlet duct and needle tubing have been inserted on the bottle lid, the one end that intake pipe and outlet duct stretch out the bottle all is equipped with the fungus strainer, and the fungus strainer of connecting the intake pipe passes through trachea and air pump intercommunication, the one end intercommunication that the needle tubing stretches out the bottle has the sample addition mouth, be equipped with the fungus strain cotton in the sample addition mouth. The utility model provides a soil ventilation culture apparatus, the device when soil nutrient changes with time in the simulation greenhouse, conveniently add the additive in order to observe its influence to soil microbial community structure, and be difficult for introducing other irrelevant bacterium to for the physicochemical property and the microecology change of soil in the greenhouse provide certain experimental data, have important realistic meaning to the development of agricultural.
Description
Technical Field
The utility model relates to a culture apparatus technical field, in particular to soil culture apparatus that ventilates.
Background
The soil is an ecological carrier on which human beings live, scientific and systematic research is carried out on the soil, and the soil has important practical significance for agricultural development and ecological environment protection. In the process of indoor soil culture, research and monitoring on the water or nutrient loss rate of soil are needed to observe the physicochemical property and the microecological change of the soil. But other irrelevant bacteria are easily introduced in the culture process, so that the experimental effect is influenced, the actual operation of the existing soil culture experimental device is more complex, and particularly, when exogenous microorganisms are researched, the experimental factors are less easily controlled.
Disclosure of Invention
To the above-mentioned prior art, the utility model provides a soil culture apparatus that ventilates, the device conveniently add the additive in order to observe its influence to soil microbial community structure when soil nutrient changes with time in the simulation greenhouse, and be difficult for introducing other irrelevant bacterium to for the physical and chemical properties and the micro-ecological change of soil in the greenhouse provide certain experimental data, have important realistic meaning to the development of agricultural.
The technical scheme of the invention is realized as follows:
the utility model provides a soil culture apparatus that ventilates, includes bottle, bottle lid, the bottle lid covers the bottleneck of bottle, intake pipe, outlet duct and needle tubing have been inserted on the bottle lid, the one end that intake pipe and outlet duct stretch out the bottle all is equipped with the fungus filter, and the fungus filter of connecting the intake pipe passes through trachea and air pump intercommunication, the one end intercommunication that the needle tubing stretches out the bottle has the sample application port, be equipped with the fungus cotton filter in the sample application port.
Furthermore, the sample adding port is conical, and the bottom of the sample adding port is communicated with the upper end of the needle tube.
Further, the bottle is a PP bottle.
Further, the outlet pipe comprises a vertical part and a horizontal part, the vertical part is arranged in the inlet pipe, and the horizontal part penetrates through the side wall of the inlet pipe and is communicated with the vertical part.
Further, the opening direction of the lower end of the air inlet pipe faces to the lower right, and the opening direction of the air outlet pipe faces to the lower left.
Furthermore, the opening of the horizontal part is arranged above, and the bacteria filter is communicated with the opening of the horizontal part.
Furthermore, the bottle also comprises an electromagnet positioned outside the bottle, the electromagnet is connected with a power supply through an adjusting switch, the bottom of the needle tube is connected with an elastic hose, the bottom of the elastic hose is connected with a metal block, and the electromagnet and the metal block interact with each other through a magnetic field.
The beneficial effects of the utility model reside in that: when exhausting, the air in the bottle is exhausted to the outside through the air outlet pipe and the bacteria filter, and the bacteria filter prevents outside bacteria from entering the bottle through the air outlet pipe. The additive penetrates through the filter bacterial cotton and then slowly enters the bottle from the needle tube, and the needle tube design is adopted, so that the stable soil ecological environment existing in the bottle body is not easily influenced by slow sample introduction. The environment in the bottle body can not be polluted by other bacteria; the filter is additionally arranged at the inlet and the outlet of the pipeline, so that the pipeline can be conveniently replaced; after the use, also can wash the arrangement to the device, reuse, and sterilization process convenient and fast. The method can be used for various detection tests, is low in cost, and can realize quantification of test work.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only preferred embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive effort.
FIG. 1 is a schematic structural view of a soil aeration culture device according to embodiment 1 of the present invention;
FIG. 2 is a schematic structural view of a soil aeration culture device according to embodiment 2 of the present invention;
in the figure, 1 bottle, 2 bottle caps, 3 air inlet pipes, 4 air outlet pipes, 5 needle tubes, 6 bacteria filters, 7 air pipes, 8 air pumps, 9 sample adding ports, 10 bacteria filter cotton, 11 vertical parts, 12 horizontal parts, 13 electromagnets, 14 adjusting switches, 15 power supplies, 16 elastic hoses and 17 metal blocks.
Detailed Description
In order to better understand the technical content of the present invention, the following embodiments are provided, and the present invention is further described with reference to the accompanying drawings.
Example 1
Referring to fig. 1, the soil aeration culture device comprises a bottle 1 and a bottle cap 2, wherein the bottle cap 2 covers a bottle opening of the bottle 1, an air inlet pipe 3, an air outlet pipe 4 and a needle tube 5 are inserted into the bottle cap 2, bacteria filters 6 are arranged at one ends of the air inlet pipe 3 and the air outlet pipe 4 extending out of the bottle 1, the bacteria filters 6 connected with the air inlet pipe 3 are communicated with an air pump 8 through an air pipe 7, one end of the needle tube 5 extending out of the bottle 1 is communicated with a sample adding opening 9, and bacteria filtering cotton 10 is arranged in the sample adding opening 9.
The bottle 1, the cover, the air inlet pipe 3, the air outlet pipe 4, the sample adding port 9, the needle tube 5 and other parts are sterilized and disinfected firstly, soil is put into the bottle 1 after the sterile state is achieved, and the cover is covered. An air inlet pipe 3, an air outlet pipe 4 and a needle tube 5 are inserted on the bottle cap 2, the air in the bottle 1 is exchanged with the outside through the air inlet pipe 4 and the air outlet pipe 4, and the additives are added into the bottle 1 from the needle tube 5. When taking a breath, the pump body works, and gets into bottle 1 with the outside air in trachea 7, strain ware 6, intake pipe 3, and strain ware 6 filters the bacterium, avoids irrelevant bacterium to get into in the bottle 1. When exhausting, air in the bottle 1 is exhausted to the outside through the air outlet pipe 4 and the bacteria filter 6, and the bacteria filter 6 prevents outside bacteria from entering the bottle 1 through the air outlet pipe 4. When the additive is added, the additive (liquid state) is put into the sample adding port 9, the additive slowly enters the bottle 1 from the needle tube 5 after permeating the filter cotton, and the stable soil ecological environment existing in the bottle body is not easily influenced by the slow sample introduction by adopting the design of the needle tube 5. The bottle body is connected with the pipeline in a seamless manner, so that the environment in the bottle body is not polluted by other bacteria; the filter 6 is additionally arranged at the inlet and the outlet of the pipeline, so that the pipeline can be conveniently replaced; optionally, the pump body is ventilated intermittently, and air circulation in the PP bottle is achieved. After the use, also can wash the arrangement to the device, reuse, and sterilization process convenient and fast. The cost is low, and the quantification of the test work can be realized.
The utility model discloses can be used for simulating the change of soil environment in the greenhouse, monitoring soil greenhouse gas emission and moisture loss situation to soil management provides certain experimental data in the greenhouse. Can be used in a variety of detection assays, for example: (1) the change conditions of soil physicochemical properties and nutrients are transferred along with time; (2) the influence of the added soil conditioner on the whole ecological environment of the soil; (3) the influence of microorganisms on the soil micro-ecological environment; (4) the morphological transformation of heavy metal elements in soil evolves over time.
Optionally, the sample addition port 9 is conical, and the bottom of the sample addition port 9 is communicated with the upper end of the needle tube 5. The substances to be added are slowly added in the sample adding port 9, and the conical structure is convenient for adding the substances.
Preferably, the bottle 1 is a PP bottle. The PP material is high-temperature and high-pressure resistant, and can be subjected to autoclave sterilization before secondary test; optionally, if there is a radiation sterilization device, PVC or other low-cost plastic material can be used to replace the sterile plastic bottle.
Preferably, the outlet duct 4 includes a vertical portion 11 and a horizontal portion 12, the vertical portion 11 is disposed in the inlet duct 3, and the horizontal portion 12 is communicated with the vertical portion 11 through a side wall of the inlet duct 3. The integrated design of the air inlet pipe 3 and the air outlet pipe 4 ensures that the pipe wall is tightly attached to the bottle cap 2, thereby greatly reducing the probability that exogenous pollutants enter the bottle body.
Preferably, the opening direction of the lower end of the intake pipe 3 is oriented to the lower right, and the opening direction of the outlet pipe 4 is oriented to the lower left. The directions of the air flows are staggered, the air in the bottle 1 has good circulating flow effect and good ventilation effect.
Preferably, the opening of the horizontal portion 12 is provided above, and the bacteria filter 6 communicates with the opening of the horizontal portion 12. The bacteria filter 6 is clamped into a horizontal opening at the upper part, so that the bacteria filter 6 is convenient to fix.
Example 2
Referring to fig. 2, the present embodiment is different from embodiment 1 in that it further comprises an electromagnet 13 located outside the bottle 1, the electromagnet 13 is connected with a power supply 15 through a regulating switch 14, an elastic hose 16 is connected to the bottom of the needle tube 5, a metal block 17 is connected to the bottom of the elastic hose 16, and the electromagnet 13 and the metal block 17 interact with each other through a magnetic field. The electromagnet 13 is mobile, not connected to the bottle 1, and acts on the metal block 17 inside the bottle 1 by means of a magnetic field. The adjusting switch 14 is turned on to switch on the electromagnet 13, the electromagnet 13 generates a magnetic field to interact with the metal block 17, and the metal block 1 drives the elastic hose 16 to move, so that the position of the elastic hose 16 is changed, and the position of adding the additive is changed. Optionally, the adjustment switch 14 is a rheostat that adjusts the power of the electromagnet 13.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The utility model provides a soil culture apparatus that ventilates, its characterized in that, includes bottle, bottle lid, the bottle lid covers the bottleneck of bottle, the bottle lid is gone up and is inserted intake pipe, outlet duct and needle tubing, the one end that intake pipe and outlet duct stretch out the bottle all is equipped with the fungus filter, and the fungus filter of connecting the intake pipe passes through trachea and air pump intercommunication, the one end intercommunication that the needle tubing stretches out the bottle has the sample addition mouth, be equipped with the fungus filter cotton in the sample addition mouth.
2. The soil aeration culture device of claim 1, wherein the sample port is tapered, and the bottom of the sample port communicates with the upper end of the needle tube.
3. A soil aeration culture device according to claim 1 wherein the bottle is a PP bottle.
4. The soil aeration culture device of claim 1, wherein the outlet pipe comprises a vertical portion provided in the inlet pipe and a horizontal portion communicating with the vertical portion through a side wall of the inlet pipe.
5. A soil aeration culture device according to claim 4, wherein the opening direction of the lower end of the air inlet pipe is oriented to the lower right, and the opening direction of the air outlet pipe is oriented to the lower left.
6. The soil aeration culture device of claim 4, wherein the opening of the horizontal part is provided above, and the bacteria filter is in communication with the opening of the horizontal part.
7. The soil aeration culture device of claim 1, further comprising an electromagnet outside the bottle, wherein the electromagnet is connected with a power supply through a regulating switch, an elastic hose is connected to the bottom of the needle tube, a metal block is connected to the bottom of the elastic hose, and the electromagnet and the metal block interact with each other through a magnetic field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020191338.0U CN211871958U (en) | 2020-02-21 | 2020-02-21 | Soil aeration culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020191338.0U CN211871958U (en) | 2020-02-21 | 2020-02-21 | Soil aeration culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211871958U true CN211871958U (en) | 2020-11-06 |
Family
ID=73251912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020191338.0U Expired - Fee Related CN211871958U (en) | 2020-02-21 | 2020-02-21 | Soil aeration culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211871958U (en) |
-
2020
- 2020-02-21 CN CN202020191338.0U patent/CN211871958U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201106 |