CN204999898U - VOCs increases bacteriolytic open fermentation cultivation device - Google Patents
VOCs increases bacteriolytic open fermentation cultivation device Download PDFInfo
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- CN204999898U CN204999898U CN201520725023.9U CN201520725023U CN204999898U CN 204999898 U CN204999898 U CN 204999898U CN 201520725023 U CN201520725023 U CN 201520725023U CN 204999898 U CN204999898 U CN 204999898U
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Abstract
The utility model discloses a VOCs increases bacteriolytic open fermentation cultivation device. Including the culture vessel 2 that aseptic technique case 1 and a plurality of connected gradually, final culture vessel 2 is located 1 outside in the aseptic technique case, and all the other culture vessels 2 all are located 1 inside in the aseptic technique case, culture vessel 2 is connected with aeration equipment 3, connect gradually and connect through flow liquid pipe 5, on managing 5, the flow liquid is provided with measuring pump 6 and valve 7, 2 usefulness of culture vessel a plurality of layers of filtering material 4 seal, and according to the order that culture vessel 2 connected gradually, the filtering material one deck that steadilys decrease is located 1 outside 2 open -endeds of culture vessel in the aseptic technique case, the drawing liquid device 9 that is provided with drain pipe 8 and connects is with it managed to the flow liquid on 5. The zymotic fluid that obtains with the device fermentation cultivation dissolves the effect excellence to increasing of VOC, and the natural fermentation mode reduction in production cost by a wide margin of intact full -open formula moreover provides technical support for BST's mass production.
Description
Technical field
The utility model belongs to biodegradation technique field.More specifically, the open type fermented culture apparatus of a kind of VOCs solubilising bacterium is related to.
Background technology
The industries such as petrochemical complex, pharmacy, paint, coating, electronic manufacture, surface anticorrosion, shoemaking, printing and communications and transportation produce a large amount of volatile organic matter (being called for short VOCs) in production and use procedure, volatile organic matter (volatileorganiccompoundsVOCs) is defined as fusing point lower than room temperature, the general name of the organic compound of boiling point between 50 ~ 260 DEG C, the VOCs identified at present reaches kind more than 300, and modal is benzene,toluene,xylene, trichloromethane, acetone and butanone etc.Many compositions of VOCs have toxicity, and what have also has carcinogenesis; If Benzene and Toluene is that VOCs relevant industries use organic solvent widely, they are present in air in a gaseous form, entering after human body can prolonged stay, even if lower concentration also can bring persistence to damage, long-term contact with it has the leukemic possibility of initiation, and benzene is classified as strong carcinogen by the World Health Organization.Due to the fast development of industry, VOCs quantity discharged and day increase severely, quantity discharged is large, kind is many because having for VOCs, strong toxicity, difficult degradation feature, become the focus of global concern.
The treatment process of VOCs is a lot, comprises condensation method, absorption process, absorption method and membrane separation process, Production by Catalytic Combustion Process, plasma method, photocatalytic oxidation and biological process etc.Mainly absorption method, Production by Catalytic Combustion Process, plasma method and biological process that current application is more.Wherein, pollutent is mainly transferred to sorbent material by absorption method, and absorption agent needs desorb, regeneration or replacing, therefore its cost is high and there is the risk of secondary pollution.Production by Catalytic Combustion Process process is relatively more thorough, but power consumption is large, and cost is high.Plasma method is in recent years for the treatment of the new technology of VOCs, but this method easily produces secondary pollution, and degradation efficiency is low, and energy consumption is high, is not suitable for industrial application.And biological process because of simple to operate, cost is low, non-secondary pollution enjoys favor, but, due to the poorly water-soluble of VOCs, cause its biodegradability on the low side, therefore limit the application of this technology.The important channel of improving VOCs biodegradability is exactly to its solubilising, and tensio-active agent has good solublization to VOCs.Utilize tensio-active agent to improve the solvability existing expansion both at home and abroad of hydrophobic organic pollutant, but most utilization is chemical surfactant, and chemical surfactant have toxicity and difficult degradation, easily causes secondary pollution.Bio-surfactant (be called for short BST) not only has good surfactivity and emulsifying property, and have nontoxic, be easy to the unique advantages such as biological degradation, make it have broad application prospects in VOCs biological treating.
Bio-surfactant is utilized to mainly contain following two kinds to VOCs solubilising at present, first method: pure bio-surfactant can be added in VOCs treatment system, but this method relates to the Isolation and purification of bio-surfactant, therefore cost is high, meanwhile, in extraction and purification process, bio-surfactant may be destroyed; Second method: can directly access the enough external source bacterium to the bio-surfactant of VOCs solubilising of production capacity in VOCs reactor, but also want first separation and purification to obtain suitable external source bacterium, and the endogenous bacterium in external source bacterium and VOCs reactor exists and competes, external source bacterium often cannot become dominant bacteria and ineffective because adaptive faculty is weak, particular surroundings particularly as VOCs, the external source bacterium of introducing is more difficult to existence.
The previous research work of the present inventor has drawn a kind of activation method that can produce the solubilising bacterium of VOC solubilizing agent, and directly use as solubilizing agent using the fermented liquid of solubilising bacterium, use simple, cost reduces greatly, technique simple, non-secondary pollution, has good using value.But this method remains the traditional microbiological cultivation and fermentation method of employing, namely need aseptically to carry out cultivation and fermentation.For large-scale Practical Project production application, obviously need everywhere aseptic condition still relatively complicated, the extra cost provided needed for gnotobasis is provided.
Summary of the invention
The technical problems to be solved in the utility model is the defect and the technical deficiency that overcome above-mentioned prior art, the open type fermented culture apparatus of a kind of VOCs solubilising bacterium is provided, the completely open spontaneous fermentation of VOCs solubilising microorganism can be realized, for the production in enormous quantities of BST provides technical support; By device of the present utility model and matching used spontaneous fermentation system, be expected to break through VOCs and affect this technical bottleneck of its biological degradation because being insoluble in water, for the high-performance bio process of VOCs provides scientific basis.
The purpose of this utility model is to provide the open type fermented culture apparatus of a kind of VOCs solubilising bacterium.
The utility model above-mentioned purpose is achieved through the following technical solutions:
An open type fermented culture apparatus for VOCs solubilising bacterium, comprises the culture vessel 2 that an aseptic operation box 1 is connected successively with several, and it is outside that last culture vessel 2 is positioned at aseptic operation box 1, and it is inner that all the other culture vessels 2 are all arranged at aseptic operation box 1; Described culture vessel 2 is connected with aerating apparatus 3 by gas flow tube, and it is inner that gas flow tube stretches into culture vessel 2 by the vents seal on aerating apparatus 3; Described connection is successively connected by fluid tube 5, and it is inner that culture vessel 2 is stretched into respectively by the perforate sealing on culture vessel 2 in fluid tube 5 two ends.
Described fluid tube 5 is provided with volume pump 6 and valve 7; Preferably, fluid tube 5 is also provided with under meter, to control the flow of bacterium liquid.
Described culture vessel 2 some layers of filtering material 4 seal, the order connected successively according to culture vessel 2, and filtering material successively decreases one deck, is positioned at last culture vessel 2 open-ended of aseptic operation box 1 outside.
Described fluid tube 5 is provided with drain pipe 8, drain pipe 8 is provided with valve, drain pipe 8 exit connects pumping device 9.Preferably, described pumping device 9 can be syringe or liquid drawing device etc.
Preferably, on described aseptic operation box 1, the position of corresponding each culture vessel 2 is provided with hand inserting hole 10; More preferably, described hand inserting hole 10 arranges Packed built-in function gloves, hand is stretched into the inner operation realized inside by handled easily person, and ensures the sealing of internal medium and aseptic.
More preferably, described aseptic operation box 1 is inner, has dividing plate to seal and separate between every two culture vessels 2, namely ensures that each culture vessel 2 is in independently in enclosed space.
This device can be used for the open type fermented cultivation of VOCs solubilising bacterium, during practical application, VOCs solubilising bacterium that is existing or that just gather first adopts shaking table to tame cultivation and obtains domestication kind of liquid, meanwhile, loads substratum and sterilizing before each culture vessel 2 in aseptic operation box 1 uses.Be inoculated in first culture vessel 2 in aseptic operation box 1 by domestication kind of liquid again, after aeration cultivates one day, Open valve 7 and volume pump 6, be forwarded to bacterium liquid in next culture vessel 2, and reduce the filtering material of one deck sealing, and so circulation is gone down.
In the process of cultivating, the valve on drain pipe 8 can be opened, extract fermented liquid by pumping device 9, to detect its emulsifying effectiveness to VOCs.When emulsifying effectiveness is stablized, the enclosed space residing for ensuing each culture vessel 2 is no longer airtight, and (no longer aseptic) completes transit working namely under field conditions (factors).
When the filtering material sealed cuts completely, can be forwarded to the open-ended culture vessel 2 of aseptic operation box 1 outside, the substratum in the open-ended culture vessel 2 of aseptic operation box 1 outside, without any sterilizing, realizes complete open spontaneous fermentation and cultivates.
Convenient in order to what operate, preferably, the connection between every two culture vessels 2 of aseptic operation box 1 inside is detachable, can increase according to the needs of practical situation and change or reduce the number of culture vessel 2.
In addition, preferably, described aseptic operation box 1 inside is provided with means of illumination and/or sterilising plant.
Preferably, described filtering material 4 is gauze, filter cotton, filter cloth or filter membrane etc.
More preferably, the aperture of described filtering material 4 is 0.01 ~ 2mm.In fact strictly speaking, strict restriction is not done in the aperture for filtering material, and which floor the little material in aperture can lack, the large material in aperture can how which floor, the requirement of test can be met equally.
In addition, preferably can embodiment as one, described culture vessel 2 is the wide-necked bottle or fermentor tank etc. of 1 ~ 5L, does not do strict restriction, can measure selection according to the actual requirements.
In addition particularly, as the enforceable scheme of one, the method that the complete open spontaneous fermentation utilizing the open type fermented culture apparatus of above-mentioned VOCs solubilising bacterium to realize VOCs solubilising bacterium is cultivated is as follows:
S1. aseptic domestication is cultivated: under aseptic condition, by the inoculum size of 2 ~ 5v/v%, the sample containing VOCs solubilising bacterium is inoculated into the fermention medium of sterilizing, and 28 ~ 32 DEG C, 170 ~ 200rpm domestication cultivation, 36 ~ 48h, obtain domestication kind of a liquid;
S2. sterile culture: according to 50 ~ 60% of vessel volume, fermention medium is added in first culture vessel 2 of aseptic operation box 1 inside, with sterilizing after the filtering material sealing that some layers are ventilative, according to the inoculum size of 2 ~ 5v/v%, switching domestication kind of liquid under aseptic condition, under 28 ~ 32 DEG C of conditions, continuous aeration is cultivated;
S3. to transfer cultivation: according to the method that step S2 is identical, cultivation of repeatedly transferring; Each switching, reduces one deck to the filtering material of closing containers;
S4. semi-open cultivation: after fermentation system is stable, becoming nature from aseptic inoculation and inoculates, namely transferring without the need to aseptically carrying out, additive method and the rapid S2 of conditional synchronization, proceeds switching and cultivates; Each switching, reduces one deck to the filtering material that culture vessel 2 seals;
S5. complete open cultivation naturally: when filtering material cuts completely, fermention medium is no longer sterilizing also, realizes complete open cultivation naturally;
Wherein, the formula of described fermention medium is: ammonium sulfate 5g/L, Spent 20g/L, glucose 1.0g/L, Repone K 1.0g/L, dipotassium hydrogen phosphate 1.5g/L, pH7 ~ 7.5, tap water constant volume;
Fermentation system described in step S4 is stable refers to stable generation tensio-active agent.The method of concrete judgement: can measure the emulsifying effectiveness of fermented liquid to VOCs, when emulsifying effectiveness is stablized, be stable generation tensio-active agent, namely fermentation system is stablized.
Preferably, each interval time of transferring is one day.
Sample containing VOCs solubilising bacterium described in step S1 can be mud, waste water or pedotheque etc.; Described mud is industrial wastewater sludge, domestic sludge or sewage ditch mud; Described waste water is trade effluent, sanitary wastewater, sewage ditch waste water; Described soil is soil near soil or service station near soil, sewage ditch near Industry Waste pond.Further, these described samples are all containing hydrocarbons.
Preferably, described in step S1 or S2, the ratio of inoculum size is 5%.
Preferably, the condition of taming cultivation described in step S1 is 30 DEG C, 36h is cultivated in 180rpm domestication.
Preferably, the condition that continuous aeration described in step S2 is cultivated is 30 DEG C of cultivations.
Preferably, fermentation culture fiduciary point culture vessel 2 volume described in step S2 60%.
In addition, what deserves to be explained is, plant bulk of the present utility model is changeable, namely aseptic operation box arrives greatly can be a sterilisable chamber, little of being a small-sized aseptic box, the requirement of variant production demand can be met, meet the demand of industrial application or laboratory study.For different demands, can on the basis not departing from thought of the present utility model and core, make corresponding amendment and improve, as: when industrial-scale is applied, namely aseptic operation box is designed to sterilisable chamber, and culture vessel is large-scale fermentor tank, then just there is not described hand inserting hole in sterilisable chamber, but need to design sealing problem according to actual needs, operator need to enter sterilisable chamber operation, and period carries out fungi-proofing work.
The present inventor affects its biological degradation problem for VOCs poorly water-soluble, is intended to domestication VOCs solubilising microorganism, makes it produce bio-surfactant (being called for short BST), to VOCs solubilising.Embodiment utilizes this new device spontaneous fermentation solubilising microorganism, represents composition using Benzene and Toluene as VOCs, using solubilization activity and emulsification rate as the solubilizing effect of index instruction fermented liquid.Result shows, the solubilising microorganism obtaining producing BST is tamed in success, and achieves complete open cultivation naturally, directly carries out solubilising with the fermented liquid obtained to VOC, not only reduce production cost, and fermented liquid also will bring nutrition for next step degrading microorganism after completing solubilising.
The completely open device that this research is set up and with it matching used spontaneous fermentation system, the production in enormous quantities that can be BST provides technical support, be expected to break through VOC and affect this bottleneck of its biological degradation because being insoluble in water, for the high-performance bio process of VOCs provides scientific basis.
The utility model has following beneficial effect:
The utility model establishes the open type fermented device of a set of VOCs solubilising microorganism and matching used completely open spontaneous fermentation system with it, the bio-surfactant (being called for short BST) that fermentation produces has excellent and stable solublization to VOCs, and, directly with the fermented liquid obtained, solubilising is carried out to VOC, not only reduce costs, and fermented liquid also will bring nutrition for next step degrading microorganism after completing solubilising.
The more important thing is, the completely open requirement of spontaneous fermentation mode to environment and equipment is lower, significantly can reduce production cost, realize large-scale engineering applications easily, for the production in enormous quantities of BST provides technical support, be expected to break through VOC and affect this bottleneck of its biological degradation because being insoluble in water, for the high-performance bio process of VOCs provides scientific basis, there is good application prospect.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the open type fermented device of VOCs solubilising microorganism of the present invention.
Fig. 2 is the external front face schematic diagram of aseptic operation box in apparatus of the present invention.
Embodiment
Further illustrate the utility model below in conjunction with the drawings and specific embodiments, but embodiment does not limit in any form to the utility model.Unless stated otherwise, the method and apparatus that the utility model adopts is the art conventional reagent, method and apparatus.
the open type fermented culture apparatus of embodiment 1VOCs solubilising bacterium
The open type fermented culture apparatus of a kind of VOCs solubilising bacterium, as depicted in figs. 1 and 2, comprise the culture vessel 2 that an aseptic operation box 1 is connected successively with several, it is outside that last culture vessel 2 is positioned at aseptic operation box 1, and it is inner that all the other culture vessels 2 are all arranged at aseptic operation box 1; Described culture vessel 2 is connected with aerating apparatus 3 by gas flow tube, and it is inner that gas flow tube stretches into culture vessel 2 by the vents seal on aerating apparatus 3; Described connection is successively connected by fluid tube 5, and it is inner that culture vessel 2 is stretched into respectively by the perforate sealing on culture vessel 2 in fluid tube 5 two ends.
Described fluid tube 5 is provided with volume pump 6 and valve 7; Preferably, fluid tube 5 is also provided with under meter, to control the flow of bacterium liquid.
Described culture vessel 2 some layers of filtering material 4 seal, the order connected successively according to culture vessel 2, and filtering material successively decreases one deck, is positioned at last culture vessel 2 open-ended of aseptic operation box 1 outside.
Described fluid tube 5 is provided with drain pipe 8, drain pipe 8 is provided with valve, drain pipe 8 exit connects pumping device 9.Described pumping device 9 can be syringe or liquid drawing device etc.
In addition, on described aseptic operation box 1, the position of corresponding each culture vessel 2 is provided with hand inserting hole 10, the convenient operation to inside.
And described aseptic operation box 1 is inner, there is dividing plate to seal between every two culture vessels 2 and separate, namely ensure that each culture vessel 2 is in independently in enclosed space.
Further, the connection between every two culture vessels 2 of aseptic operation box 1 inside is detachable, can increase according to the needs of practical situation and change or reduce the number of culture vessel 2.
This device can be used for the open type fermented cultivation of VOCs solubilising bacterium, during practical application, VOCs solubilising bacterium that is existing or that just gather first adopts shaking table to tame cultivation and obtains domestication kind of liquid, meanwhile, loads substratum and sterilizing before each culture vessel 2 in aseptic operation box 1 uses.
Be inoculated in first culture vessel 2 in aseptic operation box 1 by domestication kind of liquid again, after aeration cultivates one day, Open valve 7 and volume pump 6, be forwarded to bacterium liquid in next culture vessel 2, and reduce the filtering material of one deck sealing, and so circulation is gone down.
In the process of cultivating, the valve on drain pipe 8 can be opened, extract fermented liquid by pumping device 9, to detect its emulsifying effectiveness to VOCs.When emulsifying effectiveness is stablized, the enclosed space residing for ensuing each culture vessel 2 is no longer airtight, and (no longer aseptic) completes transit working namely under field conditions (factors).
When the filtering material sealed cuts completely, can be forwarded to the open-ended culture vessel 2 of aseptic operation box 1 outside, the substratum in the open-ended culture vessel 2 of aseptic operation box 1 outside, without any sterilizing, realizes complete open spontaneous fermentation and cultivates.
In addition, described aseptic operation box 1 inside is also provided with means of illumination and sterilising plant.
Obviously, above-described embodiment is only for the utility model example is clearly described, and is not the restriction to embodiment of the present utility model.For the person of ordinary skill of the art, the basis of above-mentioned explanation and thinking can also make other changes in different forms, exhaustive without the need to also giving all embodiments here.All do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., within the protection domain that all should be included in the utility model claim.
Claims (10)
1. the open type fermented culture apparatus of a VOCs solubilising bacterium, it is characterized in that, comprise the culture vessel (2) that an aseptic operation box (1) is connected successively with several, it is outside that last culture vessel (2) is positioned at aseptic operation box (1), and it is inner that all the other culture vessels (2) are all arranged at aseptic operation box (1); Described culture vessel (2) is connected with aerating apparatus (3) by gas flow tube, and it is inner that gas flow tube stretches into culture vessel (2) by the vents seal on aerating apparatus (3); Described connection is successively connected by fluid tube (5), and it is inner that culture vessel (2) is stretched into respectively by the perforate sealing on culture vessel (2) in fluid tube (5) two ends; Described fluid tube (5) is provided with volume pump (6) and valve (7);
Described culture vessel (2) some layers of filtering material (4) sealing, the order connected successively according to culture vessel (2), filtering material successively decreases one deck, is positioned at last culture vessel (2) open-ended that aseptic operation box (1) is outside; The pumping device (9) described fluid tube (5) being arranged drain pipe (8) and be attached thereto.
2. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 1, it is characterized in that, (8) are provided with valve to described drain pipe.
3. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 1, it is characterized in that, the connection between every two culture vessels (2) is detachable.
4. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 1, is characterized in that, the position of the upper corresponding each culture vessel (2) of described aseptic operation box (1) is provided with hand inserting hole (10).
5. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 4, is characterized in that, described hand inserting hole (10) arranges Packed built-in function gloves.
6. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 1, it is characterized in that, the inside of described aseptic operation box (1), has dividing plate to seal and separates between every two culture vessels (2), namely ensure that each culture vessel (2) is in independently in enclosed space.
7. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 1, it is characterized in that, described pumping device (9) is syringe or liquid drawing device.
8. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 1, is characterized in that, described in, described aseptic operation box (1) inside is provided with means of illumination and/or sterilising plant.
9. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 1, is characterized in that, described filtering material (4) is gauze, filter cotton, filter cloth or filter membrane.
10. the open type fermented culture apparatus of VOCs solubilising bacterium according to claim 9, it is characterized in that, the aperture of described filtering material is 0.01 ~ 2mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201520725023.9U CN204999898U (en) | 2015-09-18 | 2015-09-18 | VOCs increases bacteriolytic open fermentation cultivation device |
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| CN201520725023.9U CN204999898U (en) | 2015-09-18 | 2015-09-18 | VOCs increases bacteriolytic open fermentation cultivation device |
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| CN204999898U true CN204999898U (en) | 2016-01-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176909A (en) * | 2015-09-18 | 2015-12-23 | 中山大学 | Open type fermented cultivation method and device for VOCs solubilizing bacterium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176909A (en) * | 2015-09-18 | 2015-12-23 | 中山大学 | Open type fermented cultivation method and device for VOCs solubilizing bacterium |
| CN105176909B (en) * | 2015-09-18 | 2018-09-18 | 中山大学 | A kind of open type fermented cultural method and device of VOCs solubilisings bacterium |
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