CN209602556U - A kind of animal cells in vitro anoxic culture control device - Google Patents

Abstract

This application discloses a kind of animal cells in vitro anoxic culture control devices, air supply system including being sequentially connected setting, gas mixer, incubator, gaseous mixture escape pipe is disposed with inside the incubator from top to bottom, supporter, spacer screen, exhaust outlet, the gaseous mixture escape pipe is connected to the gas mixer, the gaseous mixture escape pipe includes multiple tube bodies of evenly distributed setting, uniformly distributed there are multiple ventholes in the side that the tube body is directed at the supporter, the supporter is provided with two rows, and it is driven by a driving chain, every layer of supporter side is provided with fan, the fan is directed at the supporter.The utility model has the advantage of the reliably repeatable anaerobic environments that efficiently can accurately simulate needed for zooblast is tested, and effectively sample are avoided to pollute, and reduce gas source waste.

Description

A kind of animal cells in vitro anoxic culture control device

Technical field

This application involves a kind of Bioexperiment device, in particular to a kind of animal cells in vitro anoxic culture control device.

Background technique

Biomedicine field carries out drug or treatment method research constantly needs to simulate in animal body under specific condition The equipment of identical cell culture environment, most widely used at present simulation cell culture environment is carbon dioxide incubator.It should The supply that equipment can automatically adjust carbon dioxide makes cell culture environment be able to maintain that the carbon dioxide set at one Concentration range, however the outer air of case is directly sucked in by blower when the supply of other gases of the equipment, it is unable to control other gas in case The proportional amount of body.In addition there are also the anaerobe cultures for cultivating the anaerobes such as helicobacter pylori on the market Equipment, such equipment need to access sample by pass box, are unsuitable for the animal cell culture operation based on fluid nutrient medium；It adopts Internal purification and sterilization, ozone remaining influence zooblast growth easy to form are carried out with ultraviolet light；Culture space is narrow, culture dish It can only stack layer by layer, be not easy to the high-volume job-lot control of sample.

Utility model content

The purpose of this utility model is to provide a kind of animal cells in vitro anoxic culture control devices, to overcome existing skill The deficiency of art.

To achieve the above object, the utility model provides the following technical solutions.

The embodiment of the present application discloses a kind of animal cells in vitro anoxic culture control device, including being sequentially connected setting Air supply system, gas mixer, incubator are disposed with gaseous mixture escape pipe, glove inside the incubator from top to bottom Frame, spacer screen, exhaust outlet, the gaseous mixture escape pipe are connected to the gas mixer, and the gaseous mixture escape pipe includes uniform Uniformly distributed there are multiple ventholes, the glove in the multiple tube bodies being arranged, the side that the tube body is directed at the supporter Erection is equipped with two rows, and is driven by a driving chain, and every layer of supporter side is provided with fan, the fan alignment The supporter.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, the air supply system includes multiple storages The bottle body of gas with various is deposited, multiple bottle body is respectively arranged with valve, and the valve is by main controller controls.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, bottom is provided in the incubator Gas sensor, side are provided with temperature sensor, and the gas sensor and temperature sensor information are sent to the master control Device processed.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, there are three the bottle body settings, respectively Storing carbon dioxide, oxygen, nitrogen.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, the gas mixer includes connection The mixing chamber of the air supply system, the mixing chamber are internally provided with multiple antibacterial hollow spheres, and the gas mixer is connected to One end of the gaseous mixture escape pipe is provided with microbe filter net.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, the gas mixer is close to described One end of incubator is provided with the first check valve.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, above the gaseous mixture escape pipe in The incubator is internally provided with electrothermal tube.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, it is arranged between two rows of supporters There is vertical clapboard.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, the incubator side is provided with one Door body, the door body upward sliding are opened.

Preferably, in above-mentioned animal cells in vitro anoxic culture control device, the incubator is in the exhaust outlet Front is disposed with second one-way valve and exhaust fan.

Compared with prior art, the utility model has the advantage of efficiently can accurately simulate zooblast experiment institute The reliably repeatable anaerobic environment needed, and effectively sample is avoided to pollute, reduce gas source waste.

Detailed description of the invention

In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this The some embodiments recorded in application, for those of ordinary skill in the art, without creative efforts, It is also possible to obtain other drawings based on these drawings.

Fig. 1 show the structural representation of animal cells in vitro anoxic culture control device in the utility model specific embodiment Figure；

Fig. 2 show the vertical view signal of animal cells in vitro anoxic culture control device in the utility model specific embodiment Figure；

Fig. 3 show the structural schematic diagram of the utility model specific embodiment middle tube body；

Fig. 4 show the side view signal of animal cells in vitro anoxic culture control device in the utility model specific embodiment Figure.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Detailed description, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole implementation Example.Based on the embodiments of the present invention, those of ordinary skill in the art institute without making creative work The every other embodiment obtained, fall within the protection scope of the utility model.

It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.

In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary, It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition The concrete meaning of language in the present invention.

In conjunction with shown in Fig. 1-4, animal cells in vitro anoxic culture control device, the air supply system including being sequentially connected setting 100, gas mixer 200, incubator 300 are disposed with gaseous mixture escape pipe 310 inside incubator 300 from top to bottom, set Rack 320, spacer screen 330, exhaust outlet 340, gaseous mixture escape pipe 310 are connected to gas mixer 200, gaseous mixture escape pipe 310 Multiple tube bodies 311 including evenly distributed setting, the side of the alignment supporter 320 of tube body 311 is uniformly distributed multiple ventholes 312, supporter 320 is provided with two rows, and is driven by a driving chain 350, and every 320 side of layer article-retaining shelf is provided with fan 360, fan 360 is directed at supporter 320.Air supply system 100 includes the bottle body 110 of multiple storage gas with various, multiple bottle body 110 are respectively arranged with valve 120, and valve 120 is controlled by master controller 130.Bottom is provided with gas sensor in incubator 300 370, side is provided with temperature sensor 380, and gas sensor 370 and 380 information of temperature sensor are sent to master controller 130.There are three the settings of bottle body 110, respectively storing carbon dioxide, oxygen, nitrogen.300 side of incubator is provided with a door body 500,500 upward sliding of door body is opened.

In the technical solution, parameter and gas sensing that automatic valve in master controller assembly is arranged according to master control panel The opening duration for summarizing operation and automatically adjusting three valves respectively of device signal, three kinds of gases of different tolerance after valve by passing through Piping enters gas mixer；Exhaust gas extracts exhaust outlet out by exhaust fan by spacer screen in case, and each layer article-retaining shelf is all provided in case There is fan, so as to gas mixing, temperature is uniform, prevents that local temperature in case is excessively high or different location gas concentration is different The problem of cause.It can be alarmed by control panel when the pressure sensor on automatic valve detects that admission pressure is too low, Prompt replacement gas source；Upward sliding opens the chamber door of the bottom when accessing sample, and supporter is under the driving of transmission chain by sample Supporter where product turns on the outside of bottom, shuts chamber door after completing access sample, and master controller opens valve according to parameter, Sterile gaseous mixture is blown out downwards by escape pipe, and the contaminated exhaust gas of bottom is blown out exhaust outlet by spacer screen；Complete exhaust gas Master control borad control valve returns to normal mode of operation after displacement, and new sample supporter is moved on to top layer by motor driven chain, favorably In the spontaneous update of new sample culture dish internal gas.

Further, gas mixer 200 includes the mixing chamber 210 of connection air supply system 100, is set inside mixing chamber 210 Multiple antibacterial hollow spheres 220 are equipped with, one end that gas mixer 200 is connected to gaseous mixture escape pipe 310 is provided with microorganism mistake Strainer 230.

In the embodiment, air-flow blows afloat the anti-biotic material hollow beads in gas mixer, by micro- after further mixing Enter gaseous mixture escape pipe after biofiltration net degerming, plays the role of uniformly mixed and degerming.

Further, gas mixer 200 is provided with the first check valve 240 close to one end of incubator 300.

In the embodiment, backflow pollution is prevented.

Further, 310 top of gaseous mixture escape pipe is internally provided with electrothermal tube 390 in incubator 300.

In the technical solution, it may be implemented to keep the temperature and heat, adapt to various environmental demand.

Further, vertical clapboard 400 is provided between two rows of supporters 320.

In the technical solution, energy optimization airflow direction reduces turbulent bring secondary pollution.

Further, incubator 300 is disposed with second one-way valve 700 and exhaust fan 600 in front of exhaust outlet 340.

Prevent gas backstreaming from polluting, and enhancing air flow power.

It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that There is also other identical elements in process, method, article or equipment including the element.

The above is only the specific embodiment of the application, it is noted that for the ordinary skill people of the art For member, under the premise of not departing from the application principle, several improvements and modifications can also be made, these improvements and modifications are also answered It is considered as the protection scope of the application.

Claims (10)

1. a kind of animal cells in vitro anoxic culture control device, which is characterized in that the air supply system including being sequentially connected setting (100), gas mixer (200), incubator (300), the incubator (300) is internal to be disposed with gaseous mixture from top to bottom Escape pipe (310), supporter (320), spacer screen (330), exhaust outlet (340), the gaseous mixture escape pipe (310) are connected to described Gas mixer (200), the gaseous mixture escape pipe (310) include multiple tube bodies (311) of evenly distributed setting, the tube body (311) side of the alignment supporter (320) is uniformly distributed has multiple ventholes (312), and the supporter (320) is provided with Two rows, and be driven by a driving chain (350), every layer of supporter (320) side is provided with fan (360), described Fan (360) is directed at the supporter (320).

2. animal cells in vitro anoxic culture control device according to claim 1, which is characterized in that the air supply system It (100) include multiple bottle bodies (110) for storing gas with various, multiple bottle body (110) is respectively arranged with valve (120), described Valve (120) is controlled by master controller (130).

3. animal cells in vitro anoxic culture control device according to claim 2, which is characterized in that the incubator (300) interior bottom is provided with gas sensor (370), and side is provided with temperature sensor (380), the gas sensor (370) master controller (130) are sent to temperature sensor (380) information.

4. animal cells in vitro anoxic culture control device according to claim 2, which is characterized in that the bottle body (110) there are three setting, difference storing carbon dioxide, oxygen, nitrogen.

5. animal cells in vitro anoxic culture control device according to claim 1, which is characterized in that the gas mixing Device (200) includes the mixing chamber (210) for connecting the air supply system (100), and the mixing chamber (210) is internally provided with multiple anti- Bacterium hollow sphere (220), one end that the gas mixer (200) is connected to the gaseous mixture escape pipe (310) are provided with micro- life Object filter screen (230).

6. animal cells in vitro anoxic culture control device according to claim 1, which is characterized in that the gas mixing Device (200) is provided with the first check valve (240) close to the one end of the incubator (300).

7. animal cells in vitro anoxic culture control device according to claim 1, which is characterized in that the gaseous mixture goes out Electrothermal tube (390) are internally provided in the incubator (300) above tracheae (310).

8. animal cells in vitro anoxic culture control device according to claim 1, which is characterized in that two rows of gloves Vertical clapboard (400) are provided between frame (320).

9. animal cells in vitro anoxic culture control device according to claim 1, which is characterized in that the incubator (300) side is provided with a door body (500), and door body (500) upward sliding is opened.

10. animal cells in vitro anoxic culture control device according to claim 1, which is characterized in that the incubator (300) second one-way valve (700) and exhaust fan (600) are disposed in front of Yu Suoshu exhaust outlet (340).