CN111849767A - Three-dimensional micro-fluidic chip level cell culture case - Google Patents
Three-dimensional micro-fluidic chip level cell culture case Download PDFInfo
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- CN111849767A CN111849767A CN202010590854.5A CN202010590854A CN111849767A CN 111849767 A CN111849767 A CN 111849767A CN 202010590854 A CN202010590854 A CN 202010590854A CN 111849767 A CN111849767 A CN 111849767A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention provides a three-dimensional microfluidic chip-level cell incubator, which comprises a culture chamber, a three-dimensional space structure microfluidic chip, a temperature control module, a gas control module and a liquid control module, wherein the three-dimensional space structure microfluidic chip, the temperature control module, the gas control module and the liquid control module are arranged in the culture chamber; the three-dimensional space structure micro-fluidic chip is constructed by utilizing liquid metal and soluble organic materials through a 3D printing technology; the temperature control module, the gas control module and the liquid control module are respectively connected with the three-dimensional space structure micro-fluidic chip. The invention can be used for simple and convenient cell culture, effectively solves the problem that the existing large-scale cell culture device has good effect but generally higher price, can provide relatively good cell growth environment when non-medical personnel want to culture cells for research, ensures stable propagation of the cells, provides convenience for research, and can also be used for simple cell culture in a laboratory.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a three-dimensional microfluidic chip-level cell incubator.
Background
In recent years, interdisciplinary communication is becoming more frequent, and medical integration is the leading edge and trend of future subject development. Especially in the manufacturing of medical instruments, the development of engineering and the updating of instruments and equipment will continuously promote the development of medicine. The cell culture box is an advanced instrument for realizing large-scale culture of cells, tissues and bacteria, is necessary equipment for developing advanced scientific researches such as immunology, oncology, genetics, bioengineering and the like in related fields such as medical science, agronomy and the like at the present stage, and is widely applied to scientific researches and production such as microorganisms, agricultural science, medical experiments and the like.
Although a large cell culture box which is put into use at present can realize accurate control of various parameters and high reliability, the large cell culture box has large size and weight, needs an external power supply during working, is inconvenient to move, but cell samples cultured in experiments often need remote transmission, the existing cell culture box is not suitable for the requirement of remote transmission, and the stability of the parameters in the cell culture box cannot be maintained in the moving process. These factors have become a great resistance to inhibit the development of relevant experiments and to hinder the development of technologies.
Therefore, there is a need for a cell culture box that can adjust and control parameters of a culture area in real time, realize constant temperature and humidity, work stably for a long time, have a small size, light weight, and are portable and can be transmitted remotely.
Disclosure of Invention
In order to solve the problems, the invention discloses a three-dimensional microfluidic chip-level cell incubator.
In order to achieve the purpose, the invention provides the following technical scheme:
a three-dimensional microfluidic chip-level cell incubator comprises a culture chamber, a three-dimensional space structure microfluidic chip arranged in the culture chamber, a temperature control module, a gas control module and a liquid control module; the three-dimensional space structure micro-fluidic chip is constructed by utilizing liquid metal and soluble organic materials through a 3D printing technology; the temperature control module, the gas control module and the liquid control module are respectively connected with the three-dimensional space structure micro-fluidic chip. Further, the temperature control module is used for maintaining the temperature in the culture chamber so as to meet the temperature requirement of cell growth.
Furthermore, the temperature control module comprises a temperature control single chip microcomputer, a heating sheet, a power amplifier switch and an infrared temperature sensor which are sequentially connected with the three-dimensional space structure microfluidic chip in series.
Further, the gas control module is a CO2 supply module for maintaining the concentration of CO2 in the culture chamber and providing the cells with gas environmental conditions necessary for survival and proliferation.
Further, the gas control module comprises a gas control single chip microcomputer, a power amplifier switch, an electromagnetic valve, a CO2 concentration sensor and a CO2 gas cylinder, wherein the gas control single chip microcomputer, the power amplifier switch, the electromagnetic valve and the CO2 concentration sensor are sequentially connected with the three-dimensional space structure microfluidic chip in series; the electromagnetic valve is arranged at the opening of the CO2 gas cylinder.
Furthermore, the liquid control module is a nutrient solution conveying module, and liquid inlet control is realized according to the growth requirements of different cells.
Further, the liquid control module comprises a liquid control single chip microcomputer, an air pump and a power amplifier switch which are sequentially connected with the three-dimensional space structure microfluidic chip in series, and further comprises a culture solution reagent bottle; the air pump is arranged at the bottle mouth of the culture solution reagent bottle.
Compared with the prior art, the invention can be used for simple and convenient cell culture, effectively solves the problem that the existing large-scale cell culture device has good effect but generally higher price, can provide relatively good cell growth environment when non-medical personnel want to culture cells for research, ensures stable cell propagation, provides convenience for research, and can also be used for simple cell culture in a laboratory.
Drawings
FIG. 1 is a schematic structural view of the present invention;
list of reference numerals: the device comprises a culture chamber 1, a three-dimensional space structure micro-fluidic chip 2, a temperature control module 3, a gas control module 4 and a liquid control module 5.
Detailed Description
The technical solutions provided by the present invention will be described in detail below with reference to specific examples, and it should be understood that the following specific embodiments are only illustrative of the present invention and are not intended to limit the scope of the present invention.
As shown in fig. 1, the invention is a schematic structural diagram of a three-dimensional microfluidic chip-scale cell incubator, and the three-dimensional microfluidic chip-scale cell incubator comprises a culture chamber 1, a three-dimensional space structure microfluidic chip 2 arranged in the culture chamber 1, a temperature control module 3, a gas control module 4, and a liquid control module 5.
The three-dimensional space structure micro-fluidic chip 2 is constructed by utilizing liquid metal and soluble organic materials and adopting a 3D printing technology.
The temperature control module 3, the gas control module 4 and the liquid control module 5 are respectively connected with the three-dimensional space structure micro-fluidic chip 2. The temperature control module 3 is used for maintaining the temperature in the culture chamber to meet the temperature requirement of cell growth. The temperature control module 3 comprises a temperature control single chip microcomputer, a heating sheet, a power amplifier switch and an infrared temperature sensor which are sequentially connected with the three-dimensional space structure micro-fluidic chip 2 in series.
The temperature control module 3 obtains the temperature of the three-dimensional structure micro-fluidic chip 2 by the temperature sensor, and the working temperature of the heating sheet is set within the range of 36.0-38.0 ℃, so that the temperature requirement of cell growth is met. In the working state, the temperature of the microfluidic chip 2 with the three-dimensional structure is obtained by the temperature sensor, and if the cell culture box is lower than 37 ℃, the temperature control single chip controls the power amplifier switch to enable the heating sheet to enter the heating state; when the temperature reaches 37 ℃, the heating sheet stops working. The working temperature threshold of the heating sheet is set at 37 ℃, so that the temperature of the three-dimensional space structure microfluidic chip 2 can be controlled within the range of 36.0-38.2 ℃, and the temperature requirement of cell growth is met.
The gas control module 4 is a CO2 supply module for maintaining the CO2 concentration in the culture chamber and providing the cells with gas environment conditions necessary for survival and proliferation. The gas control module 4 comprises a gas control single chip microcomputer, a power amplifier switch, an electromagnetic valve, a CO2 concentration sensor and a CO2 gas cylinder, wherein the gas control single chip microcomputer, the power amplifier switch, the electromagnetic valve and the CO2 concentration sensor are sequentially connected with the three-dimensional space structure microfluidic chip in series; the electromagnetic valve is arranged at the opening of the CO2 gas cylinder.
The gas control module 4 senses the concentration of carbon dioxide in the culture chamber 1 by a carbon dioxide sensor, if the concentration is lower than 5%, the gas control singlechip controls a power amplifier switch to open an electromagnetic valve, and gas with one atmosphere pressure is output after a CO2 gas cylinder passes through a pressure reducing valve. The CO2 sensor is placed on the right side of the three-dimensional structure microfluidic chip 2, so that the concentration of CO2 around the three-dimensional structure microfluidic chip 2 can be sensed conveniently. The culture chamber 1 provides the gas atmosphere conditions necessary for the survival and proliferation of the cells by stabilizing the internal CO2 concentration. The module can maintain the concentration of CO2 around the microfluidic chip 2 with a three-dimensional space structure at a stable level suitable for culture.
The liquid control module 5 is a nutrient solution delivery module, and realizes liquid inlet control according to the growth requirements of different cells. The liquid control module 5 comprises a liquid control single chip microcomputer, an air pump and a power amplifier switch which are sequentially connected with the three-dimensional space structure microfluidic chip in series, and further comprises a culture solution reagent bottle; the air pump is arranged at the bottle mouth of the culture solution reagent bottle.
The liquid control module 5 can control the liquid inlet at regular time through the liquid control singlechip according to the growth requirements of different cells. When the module enters the liquid inlet control, the power amplifier switch drives the air pump to feed air to the top of the reagent tube, the culture solution is pressed by air pressure to enter the chip, and meanwhile, the original liquid is discharged into the waste liquid tube. The measurement of the volume of the liquid in the culture solution reagent bottle and the liquid inlet speed can find that the speed is not obviously changed along with the volume of the liquid under the condition that the air pump drives the liquid inlet, and the liquid inlet amount is approximately linear. The module can replace the original liquid to realize the transportation of the culture solution.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the technical solutions, and those skilled in the art should understand that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all the modifications and equivalent substitutions should be covered by the claims of the present invention.
Claims (7)
1. The utility model provides a three-dimensional micro-fluidic chip level cell culture case which characterized in that: the device comprises a culture chamber (1), a three-dimensional space structure micro-fluidic chip (2) arranged in the culture chamber (1), a temperature control module (3), a gas control module (4) and a liquid control module (5); the three-dimensional space structure micro-fluidic chip (2) is constructed by utilizing liquid metal and soluble organic materials through a 3D printing technology; the temperature control module (3), the gas control module (4) and the liquid control module (5) are respectively connected with the three-dimensional space structure micro-fluidic chip (2).
2. The three-dimensional microfluidic chip-scale cell incubator of claim 1, wherein: the temperature control module (3) is used for maintaining the temperature in the culture chamber so as to meet the temperature requirement of cell growth.
3. The three-dimensional microfluidic chip-scale cell incubator of claim 2, wherein: the temperature control module (3) comprises a temperature control single chip microcomputer, a heating sheet, a power amplifier switch and an infrared temperature sensor which are sequentially connected with the three-dimensional space structure micro-fluidic chip in series.
4. The three-dimensional microfluidic chip-scale cell incubator of claim 1, wherein: the gas control module (4) is a CO2 supply module, is used for maintaining the concentration of CO2 in the culture chamber and provides gas environmental conditions required for survival and proliferation of cells.
5. The three-dimensional microfluidic chip-scale cell incubator of claim 4, wherein: the gas control module (4) comprises a gas control single chip microcomputer, a power amplifier switch, an electromagnetic valve, a CO2 concentration sensor and a CO2 gas cylinder, wherein the gas control single chip microcomputer, the power amplifier switch, the electromagnetic valve and the CO2 concentration sensor are sequentially connected with the three-dimensional space structure microfluidic chip in series; the electromagnetic valve is arranged at the opening of the CO2 gas cylinder.
6. The three-dimensional microfluidic chip-scale cell incubator of claim 1, wherein: the liquid control module (5) is a nutrient solution conveying module, and liquid inlet control is realized according to the growth requirements of different cells.
7. The three-dimensional microfluidic chip-scale cell incubator of claim 6, wherein: the liquid control module (5) comprises a liquid control single chip microcomputer, an air pump and a power amplifier switch which are sequentially connected with the three-dimensional space structure microfluidic chip in series, and further comprises a culture solution reagent bottle; the air pump is arranged at the bottle mouth of the culture solution reagent bottle.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010590854.5A CN111849767A (en) | 2020-06-24 | 2020-06-24 | Three-dimensional micro-fluidic chip level cell culture case |
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| CN202010590854.5A CN111849767A (en) | 2020-06-24 | 2020-06-24 | Three-dimensional micro-fluidic chip level cell culture case |
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| CN111849767A true CN111849767A (en) | 2020-10-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112501020A (en) * | 2020-12-04 | 2021-03-16 | 西北大学 | Biological tissue culture system based on micro-fluidic chip and implementation operation method thereof |
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Application publication date: 20201030 |