CN220812477U - Environment storehouse of 3D cell culture - Google Patents
Environment storehouse of 3D cell culture Download PDFInfo
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- CN220812477U CN220812477U CN202322487934.XU CN202322487934U CN220812477U CN 220812477 U CN220812477 U CN 220812477U CN 202322487934 U CN202322487934 U CN 202322487934U CN 220812477 U CN220812477 U CN 220812477U
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- 238000012604 3D cell culture Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 49
- 239000011521 glass Substances 0.000 claims abstract description 46
- 238000010438 heat treatment Methods 0.000 claims abstract description 38
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 36
- 230000007613 environmental effect Effects 0.000 claims abstract description 30
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 18
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 18
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 18
- 239000001301 oxygen Substances 0.000 claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 229910000838 Al alloy Inorganic materials 0.000 claims description 5
- 238000005260 corrosion Methods 0.000 claims description 5
- 210000004748 cultured cell Anatomy 0.000 claims description 5
- 230000006978 adaptation Effects 0.000 claims 1
- 238000004113 cell culture Methods 0.000 abstract description 20
- 238000000034 method Methods 0.000 abstract description 3
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 238000000018 DNA microarray Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 9
- 238000003384 imaging method Methods 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application discloses an environment bin for 3D cell culture, which aims to solve the problems that the existing cell culture device cannot meet the requirement of dynamic culture medium supply of a microfluidic cell chip and is inconvenient to place under a microscope for observation. The application comprises the following steps: the cultivating cabin comprises a cultivating cabin body and a computer terminal, wherein a cover plate is arranged at the top of the cabin body, light-transmitting glass is arranged on the cover plate, and heating glass is arranged at the bottom of the cabin body; the temperature and humidity monitoring module and the oxygen and carbon dioxide concentration detection sensor are arranged in the bin, the temperature sensor is arranged at the bottom of the heating glass, and the temperature sensor and the humidity sensor are connected with the computer terminal; the side wall of the bin body is provided with a liquid path connector and a pneumatic connector; the application has compact structure and convenient use, can accurately monitor and control the temperature and the humidity in the environmental chamber, well simulate the physiological environment of a human body, and has high experimental accuracy of cell culture; in the working process, a micro-fluidic chip or other biochips for cell culture are placed in the environmental bin, and the growth state of cells is observed in real time through a microscope.
Description
Technical Field
The application relates to the technical field of cell culture, in particular to an environment bin for 3D cell culture.
Background
Cell culture is an important tool in modern bioscience, which provides a key window for studying life's mystery. The meaning of cell culture is not only limited to basic research, but also plays an important role in the fields of medicine, drug development and biotechnology.
The existing cell culture mode is 2D culture, and the cell culture device is inconvenient to use, and cannot effectively adjust the environment of cell culture, such as cannot automatically adjust and monitor the temperature, humidity, atmosphere and pH in the environment culture device, so that the physiological environment of a human body cannot be well simulated, and the accuracy of experimental conclusion is affected; meanwhile, the existing cell culture apparatus cannot be directly observed under a microscope.
Disclosure of utility model
Therefore, the application provides an environment bin for 3D cell culture, which aims to solve the problems that the existing cell culture device cannot meet the requirement of dynamic culture medium supply of a microfluidic cell chip and is inconvenient to place under a microscope for observation.
In order to achieve the above object, the present application provides the following technical solutions:
An environmental chamber for 3D cell culture, comprising: the culture bin comprises a culture bin body and a computer terminal, wherein a culture bin is arranged in the bin body, and a microfluidic chip for placing cultured cells is arranged in the bin; the top of the bin body is provided with a cover plate, and the cover plate is provided with light-transmitting glass; the bottom of the bin body is provided with heating glass;
A humidity sensor is arranged in the bin; a plurality of temperature sensors are arranged inside or outside the bin body and are respectively used for detecting the overall temperature of the environment of the bin, the temperature of the heating glass and the temperature on the microfluidic chip; the temperature sensor and the humidity sensor are connected with the computer terminal;
The lateral wall of the storehouse body is provided with at least three liquid way connectors and at least one pneumatic joint, wherein: the two liquid path connectors are respectively connected with two liquid inlet pipes, culture medium and water-containing air are respectively introduced into the bin through the two liquid inlet pipes, and the other liquid path connector is connected with a liquid outlet pipe; the pneumatic connector is connected with an air inlet pipe, air is introduced into the bin through the air inlet pipe, and one end, far away from the pneumatic connector, of the air inlet pipe is connected with the air mixer; one end of the liquid inlet pipe which is introduced with the culture medium is connected with the micro-fluidic chip, and the other end is respectively connected with a liquid source.
Optionally, the light-transmitting glass is arranged in the middle of the cover plate, and the heating glass is arranged in the middle of the bottom of the bin body;
One of the temperature sensors is positioned at a middle position of the outside of the bin body corresponding to the bottom of the heating glass.
Optionally, the cover plate is connected with the interior of the bin body through hand screwing.
Optionally, the temperature sensor is connected with the computer terminal through a temperature control module.
Optionally, the microfluidic chip is disposed on the heating glass corresponding to the middle of the chamber.
Optionally, an oxygen concentration sensor and a carbon dioxide concentration sensor are further arranged in the bin, and the oxygen concentration sensor and the carbon dioxide concentration sensor are integrated on the humidity sensor.
Optionally, one end of the temperature control module is connected with the heating glass through a heating control line, and the other end of the temperature control module is connected with the computer terminal through a USB line.
Optionally, the heating glass is ITO heating glass;
the culture bin body is made of an aluminum alloy anti-corrosion bin body.
Optionally, the number of the liquid path connectors is 3-16, and the number of the pneumatic connectors is 1-2.
Optionally, a plurality of connection adapter devices are also arranged in the bin.
Compared with the prior art, the application has at least the following beneficial effects:
The application provides an environment bin for 3D cell culture, which comprises the following components: the cultivation cabin comprises a cultivation cabin body and a computer terminal, wherein a cover plate is arranged at the top of the cabin body, light-transmitting glass is arranged on the cover plate, and heating glass is arranged at the bottom of the cabin body; the humidity sensor and the microfluidic chip for placing the cultured cells are arranged in the bin, the oxygen concentration sensor and the carbon dioxide concentration sensor are integrated on the humidity sensor and are respectively used for detecting the oxygen concentration and the carbon dioxide concentration in the environmental bin, the temperature sensors are arranged outside the bin body and at the bottom of the heating glass, and the temperature sensors, the humidity sensor, the oxygen concentration sensor and the carbon dioxide concentration sensor are all connected with the computer terminal; the side wall of the bin body is provided with a liquid path connector and a pneumatic connector, the liquid path connector is used for introducing culture medium and water-containing air into the bin through a liquid inlet pipe, and the pneumatic connector is used for introducing gas into the bin through an air inlet pipe; the application has compact structure and convenient use, and the chamber is also provided with a micro-fluidic chip or other types of connection adapting devices of cell culture carriers, so that the culture solution can be supplied to cells in real time for a long time; the temperature control and monitoring in the bin of the environmental bin are controlled by a precise temperature control module and a computer terminal, the precision is +/-0.1 ℃, the temperature in the environmental bin can be precisely monitored and controlled, and meanwhile, the humidity in the environmental bin is controlled by the computer terminal, and the humidity of the environmental bin is regulated to reach the humidity required by experiments; the concentration of oxygen and carbon dioxide is controlled through the computer terminal by the arrangement of the pneumatic connector, so that the atmosphere and the pH value in the environmental bin meet the experimental requirements; the method can simulate the physiological environment of a human body well, realize the 3D culture of cells, and has high experimental accuracy of cell culture.
The bin body is an aluminum alloy anti-corrosion bin body, and the environment bin has compact overall structure, small volume and mobility; the top of the bin body is provided with light-transmitting glass, the bottom of the bin body is provided with heating glass, and the bin body is transparent up and down and can be used for transmission observation; the environmental bin can be directly placed on a platform of an upright microscope or an inverted microscope for photographing imaging or video recording, and can realize real-time imaging and analysis in the cell culture process.
Drawings
In order to more intuitively illustrate the prior art and the application, several exemplary drawings are presented below. It should be understood that the specific shape and configuration shown in the drawings are not generally considered limiting conditions in carrying out the application; for example, those skilled in the art will be able to make routine adjustments or further optimizations for the addition/subtraction/attribution division, specific shapes, positional relationships, connection modes, dimensional proportion relationships, and the like of certain units (components) based on the technical concepts and the exemplary drawings disclosed in the present application.
FIG. 1 is a schematic view of an environmental chamber according to an embodiment of the present application;
FIG. 2 is a schematic diagram of an environmental chamber according to an embodiment of the present application;
FIG. 3 is a top view of the view shown in FIG. 1;
FIG. 4 is a bottom schematic view of FIG. 1;
FIG. 5 is a schematic view of the internal structure shown in FIG. 1;
FIG. 6 is a partial schematic view of the stud shown in FIG. 5;
FIG. 7 is a schematic block diagram of a circuit provided by one embodiment of the present application;
FIG. 8 is a flow chart of air supply delivery provided in one embodiment of the present application.
Reference numerals illustrate:
1. A bin body; 101. a cover plate; 102. light-transmitting glass; 103. heating the glass; 104. a microfluidic chip; 105. a liquid path connector; 106. a pneumatic connector; 107. a convex column; 108. screwing the screw by hand;
2. A humidity sensor; 201. an oxygen concentration sensor; 202. a carbon dioxide concentration sensor; 3. a temperature sensor; 4. a temperature control module; 5. a computer terminal; 6. a pressure pump; 7. a gas mixer; 8. a humidifying module.
Detailed Description
The application will be further described in detail by means of specific embodiments with reference to the accompanying drawings.
In the description of the present application: unless otherwise indicated, the meaning of "a plurality" is two or more. The terms "first," "second," "third," and the like in this disclosure are intended to distinguish between the referenced objects without a special meaning in terms of technical connotation (e.g., should not be construed as emphasis on the degree of importance or order, etc.). The expressions "comprising", "including", "having", etc. also mean "not limited to" (certain units, components, materials, steps, etc.).
The terms such as "upper", "lower", "left", "right", "middle", etc. are generally used herein for convenience of visual understanding with reference to the drawings and are not to be construed as absolute limitations on the positional relationship of the actual product. Such changes in the relative positional relationship without departing from the technical idea of the present application are also considered as the scope of the present application.
In one embodiment of the present application, an environmental chamber for 3D cell culture, as shown in fig. 1-8, comprises: the culture bin body 1 and a computer terminal 5 outside the bin body 1, wherein a culture bin is arranged in the bin body 1, a microfluidic chip 104 for placing cultured cells is arranged in the bin, a cover plate 101 is arranged at the top of the bin body 1, and light-transmitting glass 102 is arranged on the cover plate 101 and used for illumination during imaging; the bottom of the bin body 1 is provided with heating glass 103, which provides a proper temperature environment for the environment bin and meets the imaging function of an inverted microscope;
The inside or outside of the bin body 1 is provided with a plurality of temperature sensors 3 to accurately control the temperature of the environment in the bin, the plurality of temperature sensors 3 are used for detecting the whole temperature of the environment in the bin, the temperature of the heating glass 103 and the temperature on a cell culture carrier (such as a microfluidic chip 104), and any one of the temperature sensors and a temperature control module 4 which is described below can be selected to carry out closed-loop control through a computer terminal 5 so as to keep the temperature of a target environment accurate and stable; wherein a temperature sensor for detecting the temperature of the heating glass 103 is arranged at a middle position of the outside of the bin body 1 corresponding to the bottom of the heating glass 103;
The humidity sensor 2, the oxygen concentration sensor 201 and the carbon dioxide concentration sensor 202 are arranged in the bin and are used for detecting the environmental conditions in the bin so as to culture different types of cells or study the growth conditions of the same cell in different environments by changing the environments; the oxygen concentration sensor 201 is used for detecting the oxygen concentration in the bin, the carbon dioxide concentration sensor 202 is used for detecting the carbon dioxide concentration in the bin, and the oxygen concentration sensor 201 and the carbon dioxide concentration sensor 202 are integrated on the humidity sensor 2; humidity sensor 2, oxygen concentration sensor 201, carbon dioxide concentration sensor 202 and a plurality of temperature sensors 3 are all connected with computer terminal 5 to carry out closed-loop control with the control device who appends, realize the autonomous control of environment in the bin.
The cell culture bin is also provided with a connection adapting device of a microfluidic chip or other cell culture carriers, so that an externally-attached culture medium or other biological reagents can be conveniently conveyed into the cell culture bin, and long-time culture of cells can be realized;
The environmental bin can be directly placed on a platform of an upright microscope or an inverted microscope for photographing imaging or video recording, and can realize real-time imaging and analysis in the cell culture process.
The side wall of the cartridge body 1 is provided with at least three liquid path connectors 105 and at least one pneumatic connector 106, wherein: the two liquid path connectors 105 are respectively connected with two liquid inlet pipes, namely a first liquid inlet pipe and a second liquid inlet pipe, a culture medium for culturing cells is introduced into the microfluidic chip 104 of the bin through the first liquid inlet pipe, the first liquid inlet pipe (i.e. the liquid inlet pipe for introducing the culture medium) is connected with the microfluidic chip 104, the other end of the first liquid inlet pipe is connected with a liquid storage tank for storing a liquid source (in particular the culture medium), and water-containing air is introduced into the bin through the second liquid inlet pipe and used for adjusting the humidity in the bin; the other liquid path connector is connected with a liquid outlet pipe; the pneumatic connector 106 is connected with an air inlet pipe, one end of the air inlet pipe is connected with the pneumatic connector 106, the other end of the air inlet pipe is connected with the air mixer 7, air is introduced into the bin through the air inlet pipe, and then the temperature, the humidity, the atmosphere and the pH in the bin are automatically controlled through the computer terminal 5;
As shown in fig. 7 and 8, the liquid source and the air source are driven by a liquid driving mechanism (such as a pressure pump 6) and a gas driving mechanism (such as a gas mixer 7) respectively, and corresponding valve control components are arranged on a liquid inlet pipe and an air inlet pipe; liquid driving mechanism (such as pressure pump 6), gas driving mechanism (such as gas mixer 7) are connected with computer terminal 5.
Further, as shown in fig. 7 and 8, a humidifying module 8 for supplying water-containing air to adjust humidity in the chamber is further included; the gas source comprises a plurality of gases such as gas 1 (oxygen), gas 2 (carbon dioxide) and the like, the gases are mixed in a gas mixer 7, and the mixed gases are divided into two paths: one path of gas is directly connected with the pneumatic connector 106, and gas is introduced into the bin to provide atmosphere; the other path is connected with a humidifying module 8, and the humidifying module 8 is used for introducing water-containing air into the bin through a second liquid inlet pipe.
One end of the first liquid inlet pipe except for the medium is required to be connected with the microfluidic chip 104, and one end of the other liquid inlet pipes and one end of the air inlet pipe are directly connected with the bin through the liquid path connector 105 or the pneumatic connector 106.
Preferably, as shown in fig. 1 to 4, the light-transmitting glass 102 is arranged in the middle of the cover plate 101, and the heating glass 103 is arranged in the middle of the bottom of the bin body 1; the culture bin body 1 is made of an aluminum alloy anti-corrosion bin body; the heating glass 103 is ITO heating glass, and can be heated after being electrified; the liquid inlet pipe is a capillary.
Preferably, as shown in fig. 1 and 5-6, the cover plate 101 is connected with the interior of the bin body 1 through a hand-screwed screw 108; specifically, two inner convex columns 107 are arranged at opposite corners of the inner wall of the bin body 1, mounting grooves are formed in the convex columns 107 along the longitudinal direction, hand-screwed screws 108 sequentially penetrate through mounting holes in the cover plate 101 to enter the mounting grooves, and the cover plate 101 is fixedly connected with the bin body 1 by fastening the hand-screwed screws 108.
Further preferably, the humidity sensor 2 is mounted on the left side in the bin body 1 for monitoring humidity changes in the bin of the environmental bin, humidity ranges: 5% -95%.
Still further preferably, the temperature control device also comprises a temperature control module 4 positioned outside the bin body 1, and the temperature sensor 3 is connected with the computer terminal 5 through the temperature control module 4; the temperature control module 4 has good precision of +/-0.1 ℃.
Preferably, the microfluidic chip 104 is disposed on the heating glass 103 at a middle position corresponding to the chamber.
Preferably, one end of the temperature control module 4 is connected with the heating glass 103 through a heating control line, and the other end is connected with the computer terminal 5 through a USB line.
Preferably, the number of the liquid path connectors 105 is 3-16, and the number of the pneumatic connectors 106 is 1-2, and the specific number is set according to actual requirements. In addition, the environment bin is not completely sealed, and the introduction of atmosphere is equivalent to the application of positive pressure to the interior (namely the bin) of the bin body 1, so that the pressure in the bin body 1 is higher than the external air pressure, and the environment of the bin is not polluted.
The use method of the embodiment comprises the following steps: opening the cover plate 101, putting the microfluidic chip 104 with the cultured cells placed in advance on the heating glass 103, and connecting the cover plate 101 with the bin body 1 by screwing the screw 108 by hand, so that the culture bin body 1 is in a sealed state; introducing liquid (comprising culture medium and water-containing air) and gas into the chamber through the threaded joint, the liquid inlet pipe and the air inlet pipe, electrifying the heating glass 103, and then automatically controlling the temperature, humidity, atmosphere and pH in the chamber through the computer terminal 5 to realize 3D culture of cells in combination with the cell culture chip;
The humidity sensor 2 is used for detecting the humidity in the bin, the oxygen concentration sensor 201 and the carbon dioxide concentration sensor 202 are respectively used for detecting the oxygen concentration and the carbon dioxide concentration in the environmental bin, the temperature sensor 3 is used for detecting the temperature in the bin by detecting the temperature of the heating glass 103, and when the temperature and the humidity in the bin are lower than the culture value of cells, the computer terminal 5 is used for adjusting the humidity, the temperature, the atmosphere and the pH in the bin, so that the experimental requirements are met.
The top of the environmental bin is light-transmitting glass 102, the bottom of the environmental bin is heating glass 103, and the environmental bin is transparent up and down and can be used for transmission observation; the whole environment bin is an aluminum alloy anti-corrosion bin body, has a compact structure, can be directly used on a microscope stage, and is suitable for most microscopes in the market.
Any combination of the technical features of the above embodiments may be performed (as long as there is no contradiction between the combination of the technical features), and for brevity of description, all of the possible combinations of the technical features of the above embodiments are not described; these examples, which are not explicitly written, should also be considered as being within the scope of the present description.
The application has been described above with particularity and detail in connection with general description and specific embodiments. It should be understood that numerous conventional modifications and further innovations may be made to these specific embodiments, based on the technical concepts of the present application; but these conventional modifications and further innovations may also fall within the scope of the claims of the present application as long as they do not depart from the technical spirit of the present application.
Claims (10)
1. An environmental chamber for 3D cell culture, comprising: the culture bin comprises a culture bin body and a computer terminal, wherein a culture bin is arranged in the bin body, and a microfluidic chip for placing cultured cells is arranged in the bin; the top of the bin body is provided with a cover plate, and the cover plate is provided with light-transmitting glass; the bottom of the bin body is provided with heating glass;
A humidity sensor is arranged in the bin; a plurality of temperature sensors are arranged inside or outside the bin body and are respectively used for detecting the overall temperature of the environment of the bin, the temperature of the heating glass and the temperature on the microfluidic chip; the temperature sensor and the humidity sensor are connected with the computer terminal;
The lateral wall of the storehouse body is provided with at least three liquid way connectors and at least one pneumatic joint, wherein: the two liquid path connectors are respectively connected with two liquid inlet pipes, culture medium and water-containing air are respectively introduced into the bin through the two liquid inlet pipes, and the other liquid path connector is connected with a liquid outlet pipe; the pneumatic connector is connected with an air inlet pipe, air is introduced into the bin through the air inlet pipe, and one end, far away from the pneumatic connector, of the air inlet pipe is connected with the air mixer; one end of the liquid inlet pipe which is introduced with the culture medium is connected with the micro-fluidic chip, and the other end is respectively connected with a liquid source.
2. The environmental chamber for 3D cell culture according to claim 1, wherein the light-transmitting glass is disposed in the middle of the cover plate, and the heating glass is disposed in the middle of the bottom of the chamber body;
One of the temperature sensors is positioned at a middle position of the outside of the bin body corresponding to the bottom of the heating glass.
3. The environmental chamber for 3D cell culture of claim 2, wherein the cover plate is connected to the interior of the chamber body by hand-screwed screws.
4. The environmental chamber for 3D cell culture of claim 1 or 3, wherein the temperature sensor is connected with the computer terminal through a temperature control module.
5. The environmental chamber for 3D cell culture of claim 4, wherein the microfluidic chip is disposed on the heating glass corresponding to a middle portion of the chamber.
6. The environmental chamber of 3D cell culture of claim 1, wherein an oxygen concentration sensor and a carbon dioxide concentration sensor are further disposed in the chamber, the oxygen concentration sensor and the carbon dioxide concentration sensor being integrated on the humidity sensor.
7. The environmental chamber for 3D cell culture according to claim 4, wherein one end of the temperature control module is connected with the heating glass through a heating control line, and the other end is connected with the computer terminal through a USB line.
8. The environmental chamber for 3D cell culture of claim 1, wherein the heating glass is ITO heating glass;
the culture bin body is made of an aluminum alloy anti-corrosion bin body.
9. The environmental chamber for 3D cell culture of claim 1, wherein the number of the liquid path connectors is 3-16, and the number of the pneumatic connectors is 1-2.
10. The environmental chamber for 3D cell culture of claim 1, wherein a plurality of connection adaptation means are further provided in the chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322487934.XU CN220812477U (en) | 2023-09-13 | 2023-09-13 | Environment storehouse of 3D cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322487934.XU CN220812477U (en) | 2023-09-13 | 2023-09-13 | Environment storehouse of 3D cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220812477U true CN220812477U (en) | 2024-04-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322487934.XU Active CN220812477U (en) | 2023-09-13 | 2023-09-13 | Environment storehouse of 3D cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220812477U (en) |
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2023
- 2023-09-13 CN CN202322487934.XU patent/CN220812477U/en active Active
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