CN203269947U - Miniature carbon dioxide cell incubator with fluid pipe interface and electric interface - Google Patents
Miniature carbon dioxide cell incubator with fluid pipe interface and electric interface Download PDFInfo
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- CN203269947U CN203269947U CN2013200393285U CN201320039328U CN203269947U CN 203269947 U CN203269947 U CN 203269947U CN 2013200393285 U CN2013200393285 U CN 2013200393285U CN 201320039328 U CN201320039328 U CN 201320039328U CN 203269947 U CN203269947 U CN 203269947U
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Abstract
The utility model discloses a miniature carbon dioxide cell incubator with a fluid pipe interface and an electric interface. The miniature carbon dioxide cell incubator comprises a cell box body, a sensor, a cell culture frame, a circuit control system, the fluid pipe interface, the electric interface and a human-computer interface, wherein the cell box body is orderly divided into a heating area, a cell culture area and a buffer area by a first partition board and a second partition board respectively; a heating plate is arranged inside the heating area; the cell culture area is a cell culture and control area; a gas filter device and an ultrasonic atomizing humidifier are arranged inside the buffer area; the filtered carbon dioxide gas and ultrasonic atomized vapor are respectively connected with the cell culture area through pipelines; and the liquid pipe interface and the electric interface are arranged inside the buffer area and respectively communicated with the cell culture area and the external part of the incubator through the buffer area. The miniature carbon dioxide cell incubator is small in volume, compact in structure, and suitable for integration of some rising control and test equipments required by a cell analysis technology due to the fact that the miniature carbon dioxide cell incubator includes the liquid pipe interface and the electric interface.
Description
Technical field
The utility model relates to a kind of cell culture incubator, is specifically related to a kind of small carbon dioxide cell culture incubator with liquid road and electric interfaces.
Background technology
Cell is the most basic functional unit that life entity forms, the research of the physiology of the various complexity of life entity, pathological phenomenon and mechanism is all be unable to do without research to cell and molecular level, therefore, cell culture technology has become that each research field of current life science is most crucial, most basic technology.Research at external cellular function, metabolism and molecular mechanism be unable to do without the envrionment conditions that is fit to keep the cell survival state and keeps cell eubolism level.Conventional carbon dioxide cell incubator is exactly a kind of like this scientific experiment device that can make cell keep eubolism level and growth.Cell culture incubator can accurately provide the environmental parameters such as the required temperature of cell cultures, humidity and gas composition, and this class commercialization cell culture incubator stable performance at present, response are rapidly, reliability is high, and can effectively take precautions against the pollution of cell cultures.
Along with going deep into of RESEARCH ON CELL-BIOLOGY, some emerging cell cultures, analytical technology obtain tremendous development, and typical two class technology are based on cell culture assays technology and the cell sensing technology of micro-fluidic chip.Micro-fluidic chip is the main platform that microflow control technique is realized, the resulting structure (passage, reaction chamber and some other functional component) that its device principal character is its containing fluid is at the micron order yardstick, has liquid-flow controlled, consume sample and reagent few, the characteristics such as analysis speed raising, micro-fluidic chip has huge advantage aspect cytology research: 1) the micro-fluidic chip channel size is close with the individual cells size, is convenient to cell is controlled; 2) the multidimensional network structure of micro-fluidic chip forms the environment of relative closure, more near the physiological status of cell at body; 3) micro-fluidic chip can satisfy the needs of high-throughput, high intension cell analysis; 4) realize simultaneously on micro-fluidic chip the cell sample introduction, cultivate, catch, the step such as cracking and separation detection; Because micro-fluidic chip is research advantage on unicellular and many cells level, become the focus of domestic and international cytobiology basic and applied research.Cell sensor (cell-basedsensor) is the study hotspot in analysis science and bio-science cross discipline, and it utilizes viable cell as probe unit, measures the function information of analysans; Utilize the cell sensor can continuous detecting and the physiological function of analysis of cells under external stimulus, from the biology angle, can seek state, function and the basic vital movement information of cell; From the angle of assay, can study the function and benefit to cell with the evaluation analysis thing.Susceptibility is high, selectivity good, response is rapid because cell sensor has, at present extensive application in biomedical and environmental monitoring, drug development.
The emerging cell analysis technology such as micro-fluidic chip technology and cell sensing technology often need two conditions in actual application: the one, can keep existing state and the normal physiological metabolism of cell the long period in experimentation, the 2nd, can with the effective integration of external fluid pumping equipment or electric proofing unit.Although conventional cell culture incubator can realize that accurate control and the reliability of parameters are high at present, but equipment size and quality are all larger, the more important thing is the sealing of cell cultures mucosal structure, inapplicable and outside cell is controlled, Analytical equipment integrated, limited to a certain extent actually operating and the application of these emerging technologies.
The utility model content
The purpose of this utility model is to solve above-mentioned the deficiencies in the prior art, and a kind of miniaturization carbon dioxide cell incubator is provided, and this cell culture incubator is with liquid road and electric interfaces.
For achieving the above object, the utility model is by the following technical solutions:
A kind of small carbon dioxide cell culture incubator comprises following assembly: cell casing, sensor, cell cultures frame, circuit control system and man-machine interface; According to the demand of cell cultures function, successively cell casing inner compartment is divided into respectively hot zone, cell cultures district and buffer zone 3 districts by the first partition panel and the second partition panel; Fever tablet (ptc heater) has been installed in the hot zone, and fever tablet is mainly used to heating and cultivates the temperature inside the box; Cultivation region has been placed cell cultures frame and temperature, humidity and the gas concentration lwevel sensor of being made by stainless steel; The cell cultures frame can be placed the devices such as Tissue Culture Dish (culturing bottle), micro-fluidic chip, cell sensor by corrosion-resistant and be convenient to clean stainless steel and make, and sensor is used for the environmental parameter in the Real-Time Monitoring cultivation region; Buffer zone has been placed gas-filtering device and ultrasonic atomizer, is respectively used to filter the air in carbon dioxide and humidification cell cultures district, and the carbon dioxide after filtration and steam are connected with the cell cultures district by pipeline; Buffer zone or cell cultures district are provided with liquid road interface and electric interfaces and the cell cultures district are communicated with incubator is outside; Buffer zone has also designed outer liquid road and electric interfaces, is connected with the cell cultures district by buffer zone, make cultivation region and incubator external environment carried out buffer compartment from; The liquid road interface of described buffer zone is arranged at respectively on the incubator tank wall and the second partition panel of buffer zone, and described electric interfaces is arranged at respectively on the incubator tank wall and the second partition panel of buffer zone; And the liquid road of casing external equipment is connected with the cell loader of cultivation region in incubator with circuit; Incubator becomes enclosed space by cultivating the case lid closed, seals by silica gel ring, will cultivate case lid by latching system closed; Incubator has covered the printing opacity observation area, is convenient to the observation in experimentation; Cultivating case lid is connected by coupling shaft with the cultivation casing; Whole incubator is processed by thermoplastics ABS or polycarbonate; Cell culture incubator is connected with circuit control system and man-machine interface by cable.
Better, fan also has been installed in described hot zone, can make air produce convection current, be conducive to cultivate the temperature inside the box and evenly distribute.
Better, be provided with ventilation opening on described the first partition panel, make heating zone and cell cultures district can carry out the heat conduction.
The circuit control system of described incubator is mainly used in controlling the environmental parameter of cultivation region, gather environmental parameter value in cultivation region by sensor, comprise temperature, humidity and gas concentration lwevel, compare with the parameter value that presets, calculate manipulated variable by specific technology algorithm and control actuator unit, make environmental parameter reach preset value.In actual applications, temperature control algorithm can adopt fuzzy logic PID to regulate algorithm, and the control of humidity, gas concentration lwevel can be adopted the on-off control mode.Temperature sensor can adopt thermopair, hot resistance or digital temperature sensor, and humidity sensor can adopt resistance-type or capacitive moisture sensor; Carbon dioxide sensor can adopt infrared absorption type, electric chemical formula, semiconductive ceramic formula or solid electrolytic formula.Gas concentration lwevel by the break-make of controlling the magnetic valve actuator unit realize, temperature by control heater work/stop realizing, humidity by controlling the realization of cut-offfing of ultrasonic atomizer; The incubator circuit control system adopts microcontroller as control core, its circuit control principle such as Fig. 2.
Incubator is provided with buffer zone, is used for the liquid road of casing external equipment is connected with the cell loader of cultivation region in incubator with circuit.Designed easy to draw and insert external liquid road interface and electric interfaces on incubator box body, be connected with peripheral equipment with external electric interfaces by external liquid road interface; Be connected with built-in electric interface buffer end with built-in liquid road interface buffer end by liquid connecting pipeline and junction circuit respectively at external liquid road interface buffer end and external electric interfaces buffer zone end, the liquid road of the cultivation region end of built-in liquid road interface and built-in electric interfaces and cell loader is connected with electric interfaces, thereby realizes cell loader in the incubator cultivation region and liquid and the electrical communication of external device (ED).The liquid road interface of cell culture incubator and electric interfaces are as shown in Figure 3 and Figure 4.
In a better embodiment, liquid road interface and electric interfaces directly are arranged at the cell cultures district.
The utility model has the advantages that: this incubator is can volume little, compact construction, band liquid road and electric interfaces, be suitable for some emerging cell analysis technology required control mutually integrated with test set.
Description of drawings
Fig. 1 is incubator overall construction design block diagram;
Fig. 2 is incubator circuit control principle drawing;
Fig. 3 is cell culture incubator liquid road interface;
Fig. 4 is the cell culture incubator electric interfaces.
Embodiment
Fig. 1 is the small carbon dioxide cell culture incubator of this embodiment, is made of following part: silica gel ring 1, hot zone 2, first cuts off 3, cell cultures frame 4, gas filtration bottle 5, gas piping 6, check valve 7, electricity is every valve 8, ultrasonic atomizer 9, coupling shaft 10 is cultivated case lid 11, the first snap close 12, viewing window 13, man-machine interface 14, communication cable 15, buffer zone 16, electric interfaces 17, liquid road interface 18, second cuts off 19, humidity, temperature and carbon dioxide sensor 20, cell cultures district 21, fan 22, fever tablet 23, the second snap closes 24.
Cut off the 3 and second partition 19 by first and respectively cell casing inner compartment is divided into hot zone 2, cell cultures district 21 and buffer zone 16; The interior preferred ptc heater of fever tablet 23 and the fan 22 installed in hot zone 2, fever tablet 23 is mainly used to heating and cultivates the temperature inside the box, fan 22 makes air produce convection current, being conducive to cultivate the temperature inside the box evenly distributes, first cuts off on 3 and is provided with ventilation opening, makes hot zone 2 and cell cultures district 21 can carry out the heat conduction; Cultivation region 21 has been placed cell cultures frame 4 and temperature, humidity and the gas concentration lwevel sensor 20 of being made by stainless steel; Cell cultures frame 4 can be placed the devices such as Tissue Culture Dish (culturing bottle), micro-fluidic chip, cell sensor by corrosion-resistant and be convenient to clean stainless steel and make, and sensor is used for the environmental parameter in the Real-Time Monitoring cultivation region; Buffer zone 16 has been placed gas-filtering device 5-8 and ultrasonic atomizer 9, is respectively used to filter the air in carbon dioxide and humidification cell cultures district, and the carbon dioxide after filtration and steam are connected with the cell cultures district by pipeline; Buffer zone has also designed outer liquid road interface 18 and electric interfaces 17, is connected with the cell cultures district by buffer zone, make cultivation region and incubator external environment carried out buffer compartment from; Incubator becomes enclosed space by cultivating the case lid closed, seals by silica gel ring, will cultivate case lid by latching system closed; Incubator has covered the printing opacity observation area, is convenient to the observation in experimentation; Cultivating case lid is connected by coupling shaft with the cultivation casing; Whole incubator is processed by thermoplastics ABS or polycarbonate; Cell culture incubator is connected with circuit control system and man-machine interface 14 by cable 15.
Fig. 2 is the cell culture incubator circuit control principle drawing of this embodiment.
Fig. 3 is the partial enlarged drawing of the cell culture incubator liquid road interface 18 of this embodiment, liquid road interface 18 is by incubator tank wall 25, second cuts off 19, external liquid road interface 26, external liquid road interface buffer end 28, built-in liquid road interface buffer end 27, liquid connecting pipeline 29 and built-in liquid road interface 30 consist of.
Fig. 4 is the partial enlarged drawing of the cell culture incubator electric interfaces 17 of this embodiment, electric interfaces 17 is by incubator tank wall 25, second cuts off 19, external electric interfaces buffer zone end 35, built-in electric interface buffer end 34, external electric interfaces 32, junction circuit 33 and built-in electric interfaces 31 consist of.
The cell culture incubator of this embodiment can operate by the following method:
1), based on micro-fluidic filling type cell cultivation chip
The first step: open the incubator case lid, the cell cultures box body was sterilized at ultra violet lamp in 30 minutes;
Second step: outside dioxide bottle is connected with incubator carbonic acid gas pipeline, adds the distilled water after appropriate high-temperature sterilization in ultrasonic atomizer;
The 3rd step: manually cell suspension is loaded on micro-fluidic chip at Bechtop, then micro-fluidic filling type cell cultivation chip is placed on the cell cultures frame of incubator cultivation region, with liquid line with micro-fluidic chip and built-in liquid road interface cultivation region end interface UNICOM, close the cell cultures case lid, pin by snap close;
The 4th step: open the incubator power supply, by the environmental parameter of man-machine cross section input cultivation region, set temperature is 37 ℃, and humidity is 90%RH, and gas concentration lwevel is set to 5%;
The 5th step: with liquid line with external liquid road interface outer end and liquid pumping equipment (syringe pump) UNICOM, start liquid pumping equipment (syringe pump), cell culture fluid is transported on micro-fluidic chip by the interface on incubator, simultaneously also liquid can be transported to the incubator outside by liquid road interface in the cultivation region, realize that like this liquid road of cell cultivation equipment and outside cell controlling equipment is connected.
2), electrochemistry cell sensor
The first step: open the incubator case lid, the cell cultures box body was sterilized at ultra violet lamp in 30 minutes;
Second step: outside dioxide bottle is connected with incubator carbonic acid gas pipeline, adds the distilled water after appropriate high-temperature sterilization in ultrasonic atomizer;
The 3rd step: the electrochemistry cell sensor of making is placed on the cell cultures frame of incubator cultivation region, with electric wire just electrochemistry cell sensor electrode and built-in electric interfaces cultivation region end interface UNICOM, the district drips the viable cell suspension in sensor response, close the cell cultures case lid, close by snap close;
The 4th step: open the incubator power supply, by the environmental parameter of man-machine cross section input cultivation region, set temperature is 37 ℃, and humidity is 90%RH, and gas concentration lwevel is set to 5%;
The 5th step: with external electric interfaces outer end and outside electrochemical workstation or electrical signal watch-dog, start electrochemical workstation with shielding wire, realize the cell in culturing process is carried out on-line monitoring or electricity irritation.
Claims (4)
1. small carbon dioxide cell culture incubator, comprise the cell casing, sensor, cell cultures frame, circuit control system and man-machine interface is characterized in that, will be divided into hot zone, cell cultures district and buffer zone 3 districts in the cell casing successively respectively by the first partition panel and the second partition panel; Fever tablet has been installed in the hot zone; The cell cultures district is the zone of culturing cell and has placed the cell cultures frame; Buffer zone has been placed gas-filtering device and ultrasonic atomizer, and the carbon dioxide after filtration and steam are connected with the cell cultures district by pipeline; Buffer zone or cell cultures district are provided with liquid road interface and electric interfaces and the cell cultures district are communicated with incubator is outside.
2. cell culture incubator as claimed in claim 1, wherein, the liquid road interface of described buffer zone is arranged at respectively on the incubator tank wall and the second partition panel of buffer zone, and described electric interfaces is arranged at respectively on the incubator tank wall and the second partition panel of buffer zone; And the liquid road of casing external equipment is connected with the cell loader of cultivation region in incubator with circuit.
3. cell culture incubator as claimed in claim 2, wherein, be provided with easy to draw and insert external liquid road interface and external electric interfaces on the buffer zone tank wall, is connected with peripheral equipment respectively with external electric interfaces by external liquid road interface; External liquid road interface buffer end and external electric interfaces buffer zone end are connected with built-in electric interface buffer end by the built-in liquid road interface buffer end on liquid connecting pipeline and junction circuit and buffer zone the second partition panel respectively, the liquid road of the cultivation region end of built-in liquid road interface and built-in electric interfaces and cell loader is connected with electric interfaces, thereby realizes cell loader in the incubator cultivation region and liquid road and the electrical communication of external device (ED).
4. described cell culture incubator as arbitrary in claim 1-3, wherein, incubator has covered the printing opacity observation area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013200393285U CN203269947U (en) | 2013-01-21 | 2013-01-21 | Miniature carbon dioxide cell incubator with fluid pipe interface and electric interface |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013200393285U CN203269947U (en) | 2013-01-21 | 2013-01-21 | Miniature carbon dioxide cell incubator with fluid pipe interface and electric interface |
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| CN203269947U true CN203269947U (en) | 2013-11-06 |
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| CN2013200393285U Expired - Fee Related CN203269947U (en) | 2013-01-21 | 2013-01-21 | Miniature carbon dioxide cell incubator with fluid pipe interface and electric interface |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146575A (en) * | 2013-01-21 | 2013-06-12 | 重庆医科大学附属永川医院 | Small-scale carbon dioxide cell cultivating cabinet with fluid interface and electrical interface |
| CN108300658A (en) * | 2017-12-20 | 2018-07-20 | 佛山杰致信息科技有限公司 | A kind of biological cell culturing room |
-
2013
- 2013-01-21 CN CN2013200393285U patent/CN203269947U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146575A (en) * | 2013-01-21 | 2013-06-12 | 重庆医科大学附属永川医院 | Small-scale carbon dioxide cell cultivating cabinet with fluid interface and electrical interface |
| CN108300658A (en) * | 2017-12-20 | 2018-07-20 | 佛山杰致信息科技有限公司 | A kind of biological cell culturing room |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131106 Termination date: 20150121 |
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| EXPY | Termination of patent right or utility model |