CN211689094U

CN211689094U - Cell culture box

Info

Publication number: CN211689094U
Application number: CN201922034618.0U
Authority: CN
Inventors: 宋方茗; 刘倩; 鲜艳思; 肖宇; 李凯; 林茜茜
Original assignee: Guangxi Medical University
Current assignee: Guangxi Medical University
Priority date: 2019-11-22
Filing date: 2019-11-22
Publication date: 2020-10-16
Anticipated expiration: 2029-11-22

Abstract

The utility model discloses a cell culture box, which comprises a box body and a box cover, wherein the box cover is sealed and detachably arranged on the box body, and also comprises an injection tube, a rubber plug, a sensor integrated module and a sensor signal transmission line; the sensor integration module is arranged on the inner wall of the box body and is connected with a sensor signal transmission line. The utility model can culture cells in a common room or a laboratory, and has no strict requirement on the operation environment; the culture environment of the cells can be changed according to the monitored data in the process of culturing the cells, and the environment in the cell culture box can be ensured not to be polluted; the whole structure is simple, the cell culture plate and the cell culture box can be completely replaced under certain conditions, and the cell culture plate has great market prospect.

Description

Cell culture box

Technical Field

The utility model relates to a medical science laboratory appliance technical field, concretely relates to cell culture box.

Background

At present, biological or medical laboratories adopt various cell culture instruments or instruments, such as simple and common cell culture plates, cell culture dishes and the like, and complex and multifunctional cell culture boxes. The cell culture plate and the cell culture dish have strict environmental control on a laboratory, need sterile environment, have various functions and can control environmental factors for culturing cells, but are expensive, not suitable for wide popularization and use and cannot be used in many tests (such as teaching tests). Namely, it is necessary to design a culture device which has a simple structure and can simply control the environment for culturing cells, so that the culture device can make up for the gap between the cell culture plate and the cell culture box, and the cells can be cultured in the field or in a general experimental environment.

SUMMERY OF THE UTILITY MODEL

The utility model provides a cell culture box aiming at the defects of the prior art.

In order to achieve the above object, the utility model adopts the following technical scheme:

a cell culture box comprises a box body and a box cover, wherein the box cover is hermetically and detachably arranged on the box body, the cell culture box also comprises an injection tube, a rubber plug, a sensor integration module and a sensor signal transmission line, the injection tube is arranged on the side surface of the box body, one end of the injection tube is communicated with the inside of the box body, and the other end of the box body is hermetically arranged by adopting the rubber plug; the sensor integration module is arranged on the inner wall of the box body and is connected with a sensor signal transmission line.

The utility model discloses a cell culture box adopts sealed design, cultivates the inside sterile environment that is of box, and the moisture that needs among the culture cell process, nourishment, oxygen etc. can be through inside the injection syringe toward cultivateing the box to can observe internal environment parameter through sensor collection moulding piece, including data such as humiture, oxygen concentration, carbon dioxide concentration, culture solution concentration.

Further, the sensor integration module is one or more of a temperature sensor, a humidity sensor, a gas sensor, a pH sensor and a liquid concentration detector, and the type and the number of the sensors or the detectors can be selected to be suitable according to the specific conditions of cultured cells.

Further, the injection pipe is provided on the cartridge in a downwardly inclined manner, so that the liquid or nutrients injected thereinto by the injector can completely flow along the inner wall to the bottom of the cartridge.

Furthermore, the box body is at least provided with 2 injection pipes, the number of the injection pipes can be selected according to the types of cultured cells or the types of nutrients added inwards, and the various nutrients are prevented from being mixed in a cross mode.

Further, still include the semiconductor refrigeration piece, the bottom detachable of box body install the semiconductor refrigeration piece, the upper surface of semiconductor refrigeration piece contacts with the bottom of box body, can change its inside temperature according to the temperature demand of cultivateing the cell process.

This practical advantage lies in:

1. the utility model can culture cells in a common room or a laboratory, and has no strict requirement on the operation environment;

2. the culture environment of the cells can be changed according to the monitored data in the process of culturing the cells, and the environment in the cell culture box can be ensured not to be polluted;

3. the whole structure is simple, the cell culture plate and the cell culture box can be completely replaced under certain conditions, and the cell culture plate has great market prospect.

Drawings

FIG. 1 is a perspective view of a cell culture cassette according to the present invention;

FIG. 2 is a front view of a cell culture cassette (without a lid) of the present invention;

FIG. 3 is a top view of the cell culture case (without a lid) of the present invention.

Detailed Description

The present invention is described in detail below with reference to the accompanying drawings:

in the description of the present invention, it should be noted that the directions or positional relationships indicated by "upper", "lower", "side", etc. are based on the directions or positional relationships shown in the drawings, or the directions or positional relationships that the products of the present invention are usually placed when using, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element to be referred must have a specific direction, be constructed and operated in a specific direction, and thus, should not be construed as limiting the present invention.

As shown in fig. 1-3, a cell culture box, includes box body 1, lid 2, injection tube 3, rubber buffer 4, sensor collection moulding piece 5, sensor signal transmission line 6 and semiconductor refrigeration piece 7, lid 2 sealed and detachable install on box body 1, can not use lid 2 as required when the external environment of if cultivateing is sterile environment. The left and right sides of the box body 1 are respectively provided with 1 downward-inclined injection tube 3, one end of the injection tube 3 is communicated with the inside of the box body 1, the other end of the box body 1 is sealed by a rubber plug 4, water, nutrients, acid-base solution and the like can be added into the culture box through an injector in the process of cell culture, and the inside culture environment is maintained through aseptic operation. In order to be able to observe the change of various environmental factors in the culture box visually, the sensor integration module 5 is installed on the inner wall of the box body 1, the sensor integration module 5 is connected with a sensor signal transmission line 6, the sensor signal transmission line 6 penetrates through the box body 1 and is connected with an external control device, and the control device receives monitoring data transmitted by the sensor integration module 5 and displays the monitoring data on a display of the control device. The utility model discloses a sensor collection moulding piece 5 is one or more among temperature sensor, humidity transducer, gas sensor, PH sensor, the liquid concentration detector, and it needs to be decided according to the different grade type of cultivateing the cell or purpose, the condition of cultivateing to specifically need use which kind or which kind of sensor.

In order to control the temperature inside the culture box in the culture process, the culture box is made of transparent plastic with good thermal conductivity, a semiconductor refrigerating sheet 7 (comprising a refrigerating sheet and a refrigerating sheet mounting frame) is detachably mounted at the bottom of the box body 1, the semiconductor refrigerating sheet 7 is inserted into the bottom of the box body 1 from the side surface, a corresponding clamping position is arranged at the bottom of the box body 1, and the upper surface of the semiconductor refrigerating sheet 7 is in contact with the bottom surface of the box body 1 after being mounted. The semiconductor refrigerating sheet 7 can be electrically connected with the control device, and the control device controls the semiconductor refrigerating sheet 7 to refrigerate or heat, so that the temperature in the culture box can be artificially controlled.

The utility model discloses it is more nimble during the use, little to the requirement of external environment, can use under any environment, also can be as required artificial change inside cultivation environment, whole cell culture's in-process all can be in going on in airtight aseptic environment.

Although particular embodiments of the present invention have been described and illustrated in detail, it should be noted that various changes and modifications could be made to the above-described embodiments without departing from the spirit of the invention and the scope of the appended claims.

Claims

1. The cell culture box comprises a box body (1) and a box cover (2), wherein the box cover (2) is sealed and detachably arranged on the box body (1), and is characterized by further comprising an injection tube (3), a rubber plug (4), a sensor integrated module (5) and a sensor signal transmission line (6), wherein the injection tube (3) is arranged on the side surface of the box body (1), one end of the injection tube (3) is communicated with the inside of the box body (1), and the rubber plug (4) is arranged at the other end of the box body (1) in a sealing manner; the sensor integrated module (5) is arranged on the inner wall of the box body (1), and the sensor integrated module (5) is connected with a sensor signal transmission line (6).

2. The cell culture cassette of claim 1, wherein the sensor integration module (5) is one or more of a temperature sensor, a humidity sensor, a gas sensor, a PH sensor, and a liquid concentration detector.

3. A cell culture cassette according to claim 1, wherein the injection tube (3) is arranged on the cassette body (1) in a downward inclination.

4. A cell culture cassette according to claim 3, wherein at least 2 syringes (3) are provided on the cassette body (1).

5. A cell culture box according to any one of claims 1-4, characterized by further comprising a semiconductor refrigerating sheet (7), wherein the semiconductor refrigerating sheet (7) is detachably mounted at the bottom of the box body (1), and the upper surface of the semiconductor refrigerating sheet (7) is in contact with the bottom of the box body (1).