CN213739526U - Stem cell culture bottle for cell genetic engineering - Google Patents
Stem cell culture bottle for cell genetic engineering Download PDFInfo
- Publication number
- CN213739526U CN213739526U CN202022678780.9U CN202022678780U CN213739526U CN 213739526 U CN213739526 U CN 213739526U CN 202022678780 U CN202022678780 U CN 202022678780U CN 213739526 U CN213739526 U CN 213739526U
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- China
- Prior art keywords
- bottle
- tube
- needle
- bottle cap
- cell culture
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- 210000004027 cell Anatomy 0.000 title claims abstract description 34
- 238000004113 cell culture Methods 0.000 title claims abstract description 25
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 23
- 238000010353 genetic engineering Methods 0.000 title claims abstract description 16
- 230000029058 respiratory gaseous exchange Effects 0.000 claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 13
- 230000000241 respiratory effect Effects 0.000 claims abstract description 7
- 238000001514 detection method Methods 0.000 claims description 18
- 239000000523 sample Substances 0.000 claims description 14
- 239000000565 sealant Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 8
- 230000008859 change Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 4
- 238000010367 cloning Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a stem cell culture bottle for cell genetic engineering, which comprises a bottle body, a bottle cap, an inoculating needle and a breathing tube, wherein the bottle cap is arranged at the bottle mouth of the bottle body, and the needle head of the inoculating needle penetrates into the bottle body from the bottle cap; the inoculating needle comprises a needle tube and a rubber head, the needle head of the needle tube penetrates into the bottle body through the bottle cap, and the rubber head is arranged at one end of the needle tube, which is positioned outside the bottle cap; the respiratory tube comprises a tube body, a breathable film and a sealing plug, the tube body is a structure with two through ends, the tube body runs through the bottle cap, the breathable film is arranged in the tube body, and the sealing plug is arranged at one end of the tube body, which is positioned outside the bottle cap. The stem cell culture bottle can realize the purposes of convenient inoculation and convenient liquid change, and is convenient for cell culture.
Description
Technical Field
The utility model belongs to the technical field of cell culture equipment, in particular to stem cell blake bottle for cell genetic engineering.
Background
Stem cells are a class of cells that have unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells. Over the years, as research progresses, the definition of stem cells is continuously revised and defined from different levels. Most biologists and physicians now believe that stem cells are a class of cells derived from embryonic, fetal, or adult cells that have the ability to self-renew and proliferate and differentiate without restriction under certain conditions, and that are capable of producing daughter cells with the same phenotype and genotype as themselves, and also capable of producing specialized cells that make up body tissues and organs, and also capable of differentiating into progenitor cells.
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.
Culture bottles are often needed in the stem cell culture process, but most of the existing culture bottles have the following defects:
(1) the inoculation device is lacked, so that the inoculation is inconvenient;
(2) lack detection device, can not real-time detection bottle internal environment, lead to cultivateing the failure easily.
Based on this, the present inventors have made research and improvement on this, and the present application has resulted therefrom.
SUMMERY OF THE UTILITY MODEL
The main object of the utility model is to provide a stem cell blake bottle for cell genetic engineering, its purpose that can realize being convenient for the inoculation, be convenient for trade liquid is convenient for carry out cell culture.
The utility model discloses a secondary purpose lies in providing a stem cell blake bottle for cell genetic engineering, and it can realize real-time detection and cultivate the environment, improves and cultivates the effect.
In order to achieve the above purpose, the solution of the present invention is:
a stem cell culture bottle for cell genetic engineering comprises a bottle body, a bottle cap, an inoculating needle and a breathing tube, wherein the bottle cap is arranged at the bottle opening of the bottle body, and the needle head of the inoculating needle penetrates into the bottle body from the bottle cap;
the inoculating needle comprises a needle tube and a rubber head, the needle head of the needle tube penetrates into the bottle body through the bottle cap, and the rubber head is arranged at one end of the needle tube, which is positioned outside the bottle cap;
the respiratory tube comprises a tube body, a breathable film and a sealing plug, the tube body is a structure with two through ends, the tube body runs through the bottle cap, the breathable film is arranged in the tube body, and the sealing plug is arranged at one end of the tube body, which is positioned outside the bottle cap.
The needle tube of the inoculating needle is provided with a plurality of detection probes at the part positioned in the bottle body, a storage battery and a processor are arranged in the bottle cap, a display is arranged at the outer side of the bottle cap, and the storage battery supplies power for the processor and the display; the detection probe senses parameters in the bottle body, transmits the parameters to the processor, and then sends the parameters to the display for displaying.
The detection probe comprises a pH value detection sensor, a temperature detection sensor, a humidity detection sensor and a pressure detection sensor, and is uniformly arranged on the periphery of the needle tube.
The bottle cap is provided with a groove at the position corresponding to the bottle mouth of the bottle body, and the groove is matched with the bottle mouth.
The groove of the bottle cap is provided with a first through hole at the center, and the needle tube of the inoculating needle passes through the first through hole and enters the bottle body.
And a sealant is filled between the needle tube of the inoculating needle and the first through hole.
A second through hole is formed in the groove of the bottle cap, and the pipe body of the breathing pipe penetrates through the second through hole.
After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is:
(1) the utility model is convenient for experimenters to inoculate by arranging the inoculating needle through the bottle cap, and when liquid needs to be changed, the liquid can be changed by taking down the rubber head, thus being convenient for use;
(2) the utility model discloses a set up a plurality of test probes on the inoculating needle, conveniently monitor the interior culture environment of bottle, and then make things convenient for the experimenter to adjust the interior environment of blake bottle, improve and cultivate the success rate;
(3) the utility model discloses an at respiratory tube internally mounted ventilated membrane, effectively completely cut off external bacterial contamination bottle inner cell, and set up the sealing plug in respiratory tube one side, conveniently open and close the respiratory tube to the exchange of the outer bottle gas in the convenient control bottle.
Drawings
Fig. 1 is a schematic front structural view of the present invention;
FIG. 2 is a schematic view of the structure of the inoculating needle of the present invention;
FIG. 3 is a schematic view of the structure of the respiratory tube of the present invention;
fig. 4 is a schematic view of the external structure of the bottle cap of the present invention.
Reference numerals:
1. a bottle body; 2. an inoculating needle; 3. a bottle cap;
4. a storage battery; 5. a breathing tube; 6. a processor;
7. a needle tube; 8. detecting a probe; 9. a rubber head;
10. a pipe body; 11. a gas permeable membrane; 12. a sealing plug;
13. a display; 14. a control key;
15. a first through hole; 16. a second through hole; 17. and (4) a groove.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
As shown in fig. 1, the present invention provides a stem cell culture bottle for cell genetic engineering, which comprises a bottle body 1, a bottle cap 3 is fixedly installed on one side of the bottle body 1, a breathing tube 5 penetrates through the bottom of the bottle cap 3 close to an inoculating needle 2, and can be matched with the fig. 3, wherein the breathing tube 5 comprises a tube body 10, a breathable film 11 and a sealing plug 12; the storage battery 4 is fixedly mounted at the bottom of the inner side of the bottle cap 3, the processor 6 is fixedly mounted at the top of the inner side of the bottle cap 3, the display 13 is fixedly mounted at one side, close to the processor 6, of the outer portion of the bottle cap 3, a plurality of control keys 14 are arranged at one side of the display 13, and the storage battery 4, the display 13 and the control keys 14 are electrically connected with the processor 6 through wires.
The inside inoculation needle 2 that has run through of bottle lid 3, as shown in fig. 2, inoculation needle 2 includes needle tubing 7, test probe 8 and rubber head 9, and test probe 8 is provided with a plurality ofly and evenly arranges the lateral wall at needle tubing 7, and rubber head 9 fixed mounting is in the one side of keeping away from the 2 syringe needles of inoculation needle.
As a preferred embodiment of the present invention, the inside fixed mounting of the detecting probe 8 has a ph detecting sensor, a temperature detecting sensor, a humidity detecting sensor and a pressure detecting sensor, and the detecting probe 8 is electrically connected to the processor 6.
As a preferred embodiment of the present invention, as shown in fig. 3, the breathing tube 5 penetrates the bottle cap 3 to communicate the space inside the bottle with the space outside the bottle, the air permeable membrane 11 is fixedly installed inside the tube 10, and the sealing plug 12 is fixedly installed at one end of the tube 10 close to the space outside the bottle.
As a preferred embodiment of the present invention, as shown in fig. 4, a groove 17 is formed on one side of the bottle cap 3, and the groove 17 is engaged with the opening of the bottle body 1 for sealing.
As a preferred embodiment of the present invention, as shown in FIG. 4, a first through hole 15 is opened at the center of the groove 17, the inoculating needle 2 is fixedly installed inside the first through hole 15, and the joint between the first through hole 15 and the inoculating needle 2 is filled with a sealant.
As a preferred embodiment of the present invention, as shown in fig. 4, the second through hole 16 is opened on one side of the bottom of the groove 17 near the first through hole 15, the breathing tube 5 is fixedly installed inside the second through hole 16, and the outer wall of the tube body 10 is tightly attached to the second through hole 16.
The utility model discloses a theory of operation is: when the culture bottle works, the bottle cap 3 is taken down, stem cells are inoculated into the culture bottle by using the inoculating needle 2, then the bottle cap 3 is installed on the bottle body 1, the detection probe 8 on the inoculating needle 2 can detect the environmental parameters in the bottle in real time and send the environmental parameters into the processor 6, the processor 6 is converted into a format suitable for the display 13 and sends the format to the display 13 for displaying various parameters, an experimenter can monitor the internal environment of the culture bottle through the display 13 and can also control the display content of the display 13 through the control key 14, such as only displaying a certain environmental parameter, switching the display mode and the like; when liquid needs to be changed, the rubber head 9 is taken down for liquid change, and when air needs to be changed, the sealing plug 12 is taken down for air change; the stem cell culture bottle for cell genetic engineering is convenient to inoculate by installing the inoculating needle 2, and when liquid needs to be changed, the liquid can be changed by taking down the rubber head 9, so that the use is convenient; the detection probe 8 is arranged on the inoculating needle 2, so that the culture environment in the bottle can be monitored conveniently, experimenters can adjust the internal environment of the culture bottle conveniently, and the success rate of culture is improved; the breathable film 11 is arranged in the breathing tube 5, so that the pollution of external bacteria to cells in the bottle is effectively isolated, and the sealing plug 12 is arranged on the side of the breathing tube 5, so that the breathing tube 5 is convenient to open and close, and the exchange of gas outside the bottle in the bottle is convenient to control; the device simple structure is reasonable, and convenient to use has effectively solved the problem that present majority blake bottle lacks detection device and lacks inoculation device, has higher practical value.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (7)
1. A stem cell culture bottle for cell genetic engineering is characterized in that: comprises a bottle body, a bottle cap, an inoculating needle and a breathing tube, wherein the bottle cap is arranged at the bottle mouth of the bottle body, and the needle head of the inoculating needle penetrates into the bottle body from the bottle cap;
the inoculating needle comprises a needle tube and a rubber head, the needle head of the needle tube penetrates into the bottle body through the bottle cap, and the rubber head is arranged at one end of the needle tube, which is positioned outside the bottle cap;
the respiratory tube comprises a tube body, a breathable film and a sealing plug, the tube body is a structure with two through ends, the tube body runs through the bottle cap, the breathable film is arranged in the tube body, and the sealing plug is arranged at one end of the tube body, which is positioned outside the bottle cap.
2. The stem cell culture flask for cell genetic engineering according to claim 1, wherein: the needle tube of the inoculating needle is provided with a plurality of detection probes at the part positioned in the bottle body, a storage battery and a processor are arranged in the bottle cap, a display is arranged on the outer side of the bottle cap, and the storage battery supplies power for the processor and the display; the detection probe senses parameters in the bottle body, transmits the parameters to the processor, and then sends the parameters to the display for displaying.
3. The stem cell culture flask for cell genetic engineering according to claim 2, wherein: the detection probe comprises a pH value detection sensor, a temperature detection sensor, a humidity detection sensor and a pressure detection sensor, and is uniformly arranged on the periphery of the needle tube.
4. The stem cell culture flask for cell genetic engineering according to claim 1, wherein: the bottle cap is provided with a groove at the position corresponding to the bottle mouth of the bottle body, and the groove is matched with the bottle mouth.
5. The stem cell culture flask for cell genetic engineering according to claim 4, wherein: a first through hole is formed in the center of the groove of the bottle cap, and a needle tube of the inoculating needle penetrates through the first through hole to enter the bottle body.
6. The stem cell culture flask for cell genetic engineering according to claim 5, wherein: and a sealant is filled between the needle tube of the inoculating needle and the first through hole.
7. The stem cell culture flask for cell genetic engineering according to claim 4, wherein: a second through hole is formed in the groove of the bottle cap, and the pipe body of the breathing pipe penetrates through the second through hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022678780.9U CN213739526U (en) | 2020-11-18 | 2020-11-18 | Stem cell culture bottle for cell genetic engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022678780.9U CN213739526U (en) | 2020-11-18 | 2020-11-18 | Stem cell culture bottle for cell genetic engineering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213739526U true CN213739526U (en) | 2021-07-20 |
Family
ID=76825958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022678780.9U Expired - Fee Related CN213739526U (en) | 2020-11-18 | 2020-11-18 | Stem cell culture bottle for cell genetic engineering |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213739526U (en) |
-
2020
- 2020-11-18 CN CN202022678780.9U patent/CN213739526U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210720 |