CN217868918U

CN217868918U - Cell culture dish

Info

Publication number: CN217868918U
Application number: CN202222026412.5U
Authority: CN
Inventors: 赵红叶; 刘孟歆; 徐蕊
Original assignee: Hebei Insaile Biotechnology Co ltd
Current assignee: Hebei Insaile Biotechnology Co ltd
Priority date: 2022-08-03
Filing date: 2022-08-03
Publication date: 2022-11-22
Anticipated expiration: 2032-08-03

Abstract

The utility model discloses a cell culture dish, which comprises a shell, wherein a cavity is arranged in the shell; one end of the shell is connected with a neck, and the joint of the neck and the shell is an inclined and smooth inclined plane; the other end of the neck part is gradually cylindrical, and a threaded port is arranged on the outer wall of the cylindrical part; the threaded opening is provided with a cover body, and a plurality of through holes are formed in the cover body; the through holes are covered with gas-permeable films through which only gas passes and liquid cannot pass; a tail pipe is communicated with the side wall of the shell opposite to the neck part and is connected with a connecting pipe; the other end of the connecting pipe is connected with a gas cylinder, and a flow controller for adjusting the gas flow speed is arranged between the gas cylinder and the shell. The utility model has the advantages of simple structure, convenient operation and effective reduction of gas consumption.

Description

Cell culture dish

Technical Field

The utility model relates to a cell biology technical field, more specifically relate to a cell culture dish.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, proper pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function.

Cell culture is generally performed on a culture dish, and the culture dish is basically divided into two types, plastic and glass, from the material. Some culture conditions need to provide a stable gas atmosphere, and only a culture bottle can be placed in an incubator to obtain a required gas environment, but it is inconvenient for an experimenter to observe changes on a culture dish cell group.

Chinese patent CN 207294809U provides a neural stem cell culture bottle, which is provided with a permeable pipeline for gas and liquid to permeate into the cell culture space to supplement gas and nutrients, but the structure in the culture bottle is complex, and cells are easily attached to the pipeline, which results in partial cell loss.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the technical problem that needs to solve provides a cell culture dish to solve the problem in the background art.

In order to solve the technical problem, the utility model adopts the following technical proposal.

The cell culture dish comprises a shell with a cavity arranged inside; one end of the shell is connected with a neck, and the joint of the neck and the shell is an inclined and smooth inclined plane; the other end of the neck part is gradually cylindrical, and a threaded port is formed in the outer wall of the cylindrical part; the threaded port is provided with a cover body, and a plurality of through holes are formed in the cover body; the through holes are covered with gas-permeable films which only allow gas to pass through but not allow liquid to pass through; a tail pipe is communicated with the side wall of the shell opposite to the neck part and is connected with a connecting pipe; the other end of the connecting pipe is connected with a gas cylinder, and a flow controller used for adjusting the air flow speed is arranged between the gas cylinder and the shell.

Further optimize technical scheme, the casing is glass or plastics material.

Further optimize technical scheme, the bight of casing inner wall all adopts circular arc design.

The technical scheme is further optimized, and the breathable film on the cover body is a film with hydrophobicity.

Further optimize technical scheme, be provided with the check valve on the connecting pipe between gas cylinder and the flow controller.

Due to the adoption of the technical scheme, the utility model has the following technical progress.

The utility model provides a pair of cell culture dish is through the tail pipe that sets up and inner chamber intercommunication on the culture dish casing to cooperation gas cylinder, flow controller realize having practiced thrift gaseous use amount to the regulation of cell culture gas atmosphere, also the experimenter of being more convenient for observes at the cultivation in-process. The utility model has the advantages of simple structure, convenient operation and capability of effectively reducing the gas consumption.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a housing portion of the present invention;

wherein: 1. the device comprises a shell, 2, a neck, 3, a threaded port, 4, a cover body, 5, a through hole, 6, a breathable film, 8, a tail pipe, 9, a connecting pipe, 10, a flow controller, 11, a gas bottle, 12 and a one-way valve.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

The cell culture dish, as shown in fig. 1 to 2, includes a casing 1, a neck 2, a screw port 3, a cover 4, a through hole 5, a gas permeable membrane 6, a tail tube 8, a connecting tube 9, a flow controller 10, a gas cylinder 11, and a check valve 12.

The casing 1 is made of glass or plastic, and a cavity for culturing cells is arranged in the casing 1. The corners of the inner wall of the shell 1 are designed to be circular arc-shaped so as to reduce the contact dead angle when the suction pipe blows and beats cells.

One end of the shell 1 is connected with a neck part 2, and the joint of the neck part 2 and the shell 1 is an inclined and smooth inclined plane. The other end of the neck part 2 gradually shrinks and finally takes a cylindrical shape, and a threaded port 3 is arranged on the outer wall of the cylindrical part. The screw port 3 is provided with a lid 4.

A plurality of through holes 5 are arranged on the cover body 4, and the through holes 5 are covered with breathable films 6 which only allow gas to pass through but prevent liquid from passing through. The breathable film 6 on the cover body 4 is designed to be hydrophobic, so that the tightness and the breathable effect of the breathable film 6 are not affected after the breathable film 6 is contacted with liquid.

The side wall of the shell 1 opposite to the neck 2 is communicated with a tail pipe 8, and the tail pipe 8 is connected with a connecting pipe 9. The other end of the connecting pipe 9 is connected with an air bottle 11, a flow controller 10 is arranged between the air bottle 11 and the shell 1, and the flow controller 10 is used for adjusting the speed of the air flow. A check valve 12 is provided on the connection pipe 9 between the gas cylinder 11 and the flow controller 10.

The utility model discloses when in actual use, at first open lid 4, the experimenter puts into casing 1 the cell that will cultivate, then screws lid 4. Selecting a proper gas bottle 11 according to the experiment requirement, connecting the gas bottle 11 with a flow controller 10, then connecting the gas bottle 11 with a tail pipe 8 on the shell 1, and enabling the gas to flow into the shell 1 according to the experiment setting parameters under the regulation of the flow controller 10.

Claims

1. Cell culture dish, its characterized in that: comprises a shell (1) with a cavity arranged inside; one end of the shell (1) is connected with a neck part (2), and the joint of the neck part (2) and the shell (1) is an inclined and smooth inclined plane; the other end of the neck part (2) is gradually cylindrical, and a threaded port (3) is formed in the outer wall of the cylindrical part; the threaded opening (3) is provided with a cover body (4), and the cover body (4) is provided with a plurality of through holes (5); the through holes (5) are covered with gas-permeable membranes (6) which only allow gas to pass through but not allow liquid to pass through; a tail pipe (8) is communicated with the side wall of the shell (1) opposite to the neck (2), and the tail pipe (8) is connected with a connecting pipe (9); the other end of the connecting pipe (9) is connected with a gas cylinder (11), and a flow controller (10) for adjusting the air flow speed is arranged between the gas cylinder (11) and the shell (1).

2. A cell culture dish according to claim 1, wherein: the shell (1) is made of glass or plastic.

3. A cell culture dish according to claim 2, wherein: the corner of the inner wall of the shell (1) is designed in a circular arc shape.

4. A cell culture dish according to claim 1, wherein: the air permeable membrane (6) on the cover body (4) is a membrane with hydrophobicity.

5. A cell culture dish according to claim 1, wherein: a one-way valve (12) is arranged on a connecting pipe (9) between the gas cylinder (11) and the flow controller (10).