CN206266605U - A kind of blake bottle air interchanger - Google Patents

Abstract

The utility model provides a ventilation device for a culture bottle, which can control the air intake rate in the culture bottle, so as to ensure the gas concentration of the culture solution and prevent the pollution of miscellaneous bacteria. The culture bottle ventilation device includes a culture bottle body, a sealing gasket provided on the mouth of the culture bottle body and a bottle cap for pressing the middle through hole of the sealing gasket, and the sealing gasket is pierced with a ventilation tube And the air inlet pipe, the upper part of the air exchange pipe is connected with the membrane filter, and the lower outlet of the air inlet pipe is close to the bottom of the culture bottle body. The upper part of the ventilation pipe of the utility model is connected with the diaphragm filter, because the pores of the diaphragm filter are evenly distributed, which can prevent the contamination of bacteria, and the lower outlet of the air inlet pipe is close to the bottom of the culture bottle body, when the air is fed in, the The trachea is directly inserted into the culture solution, so that the intake rate of the culture bottle can be controlled, and the gas solubility of the culture solution can be greatly improved, thereby ensuring the gas concentration of the culture solution, which is beneficial to the cultivation of microorganisms.

Description

A ventilation device for a culture bottle

technical field

The utility model relates to the field of biological engineering appliances, in particular to a ventilation device for a culture bottle.

Background technique

The multi-channel strain screening and optimization system is based on a biological constant temperature incubator as a prototype, which can measure and control the pH of each culture bottle and add external nutrient solution. It is suitable for strain screening of microorganisms, animal and plant cells, seed expansion, cultivation and The optimization of its medium and so on. In the existing biological constant temperature incubator, the ventilation of the culture bottle is made of common bottle stoppers such as cotton plugs, resulting in a slow exchange rate between the air in the culture bottle and the outside world, and the low solubility of the gas in the culture solution, which is not conducive to microorganisms. In addition, the stomata of ordinary bottle stoppers are unevenly distributed, and the size of the stomata is uncontrollable, which is easily contaminated by bacteria.

Contents of the invention

The purpose of this utility model is to propose a ventilation device for a culture bottle, which can control the air intake rate in the culture bottle, so as to ensure the gas concentration of the culture solution and prevent the contamination of bacteria.

According to the culture bottle ventilation device provided by the utility model, it includes a culture bottle body, and the key is to also include a sealing gasket provided on the bottle mouth of the culture bottle body and a bottle cap for pressing the middle through hole of the sealing gasket, The sealing gasket is pierced with a ventilation tube and an air intake tube, the upper part of the ventilation tube is connected with a membrane filter, and the outlet of the lower part of the air intake tube is close to the bottom of the culture bottle body.

The ventilation device for the culture bottle of the utility model keeps the inside of the culture bottle isolated from the outside air through the sealing gasket, and the ventilation pipe and the air inlet pipe passing through the sealing gasket are responsible for the ventilation and air intake in the culture bottle respectively. The upper part of the air exchange tube is connected to the membrane filter. Because the pores of the membrane filter are evenly distributed, it can prevent the contamination of bacteria. The lower outlet of the air intake tube is close to the bottom of the culture bottle body. Liquid, so that the air intake rate of the culture bottle can be controlled, and can greatly improve the gas solubility of the culture solution, thereby ensuring the gas concentration of the culture solution, which is conducive to the cultivation of microorganisms.

Further, the bottle cap is threadedly connected to the mouth of the culture bottle body. By screwing the bottle cap and the bottle mouth, the sealing gasket can be guaranteed to be pressed tightly, even if the sealing gasket is aging, it is enough to ensure the sealing of the bottle mouth, and the threaded connection is convenient for installation and disassembly.

Specifically, the culture bottle body is an Erlenmeyer flask, and the side walls of the culture bottle body are respectively provided with a feeding port for adjusting the pH of the culture solution and an opening for installing a pH sensor.

Description of drawings

Fig. 1 is the structural representation of the utility model.

detailed description

Below with reference to the accompanying drawings, through the description of the implementation examples, the specific embodiments of the present utility model, such as the shape, structure, mutual position and connection relationship between each part, the role and working principle of each part, etc. Further details.

As shown in Figure 1, the culture bottle ventilation device of the present utility model includes a culture bottle body 1, a sealing gasket 2 provided at the mouth of the culture bottle body 1 and a bottle for pressing the middle through hole of the sealing gasket 2. The cover 3 and the sealing gasket 3 are perforated with a ventilation tube 4 and an air intake tube 5, the upper part of the ventilation tube 4 is connected with the membrane filter 6, and the lower outlet of the air intake tube 5 is close to the bottom of the culture bottle body 1. As a preferred embodiment, the bottle cap 3 is connected to the bottle mouth of the culture bottle body 1 through threads. By threading the bottle cap 3 and the bottle mouth, it is possible to ensure that the sealing gasket 2 is pressed tightly, even if the sealing gasket 2 is aging, it is enough to ensure the sealing of the bottle mouth, and the threaded connection is convenient for installation and disassembly. The culture bottle body 1 is an Erlenmeyer flask, and the side wall of the culture bottle body 1 is respectively provided with a feeding port 11 for adjusting the pH of the culture solution and an opening 12 for installing a pH sensor.

The ventilation device for the culture bottle of the present utility model keeps the inside of the culture bottle 1 isolated from the outside air through the sealing gasket 2, and the ventilation pipe 4 and the air inlet pipe 5 passing through the sealing gasket 2 are respectively responsible for the ventilation in the culture bottle. Gas and air intake, the upper part of the ventilation pipe 4 is connected with the membrane filter 6, because the pores of the membrane filter 6 are evenly distributed, which can prevent the contamination of bacteria, and the lower outlet of the air inlet pipe 5 is close to the bottom of the culture bottle body 1 , during air intake, the intake pipe 5 is directly inserted into the culture solution, so that the intake rate of the culture bottle 1 is controllable, and the gas solubility of the culture solution can be greatly improved, thereby ensuring the gas concentration of the culture solution, which is beneficial to the cultivation of microorganisms.

Claims (3)

1. a culture bottle ventilation device, comprising a culture bottle body, is characterized in that also comprising the sealing gasket that the bottle mouth of the culture bottle body is provided with and the bottle cap that is used to compress the middle part through hole of said sealing gasket, so The sealing gasket is pierced with a ventilation tube and an air intake tube, the upper part of the ventilation tube is connected with a membrane filter, and the outlet of the lower part of the gas intake tube is close to the bottom of the culture bottle body. 2. The ventilation device for the culture bottle according to claim 1, characterized in that the bottle cap is connected to the mouth of the culture bottle body by threads. 3. The culture bottle ventilation device according to claim 1 or 2, characterized in that the culture bottle body is an Erlenmeyer flask, and the bottle side walls of the culture bottle body are respectively provided with a supplement for adjusting the pH of the culture solution. Spout and opening for pH sensor.