CN106434314A - Culture bottle air change device - Google Patents
Culture bottle air change device Download PDFInfo
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- CN106434314A CN106434314A CN201610945100.0A CN201610945100A CN106434314A CN 106434314 A CN106434314 A CN 106434314A CN 201610945100 A CN201610945100 A CN 201610945100A CN 106434314 A CN106434314 A CN 106434314A
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- culture bottle
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- 238000009423 ventilation Methods 0.000 claims abstract description 25
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 239000013589 supplement Substances 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 6
- 238000011109 contamination Methods 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000011148 porous material Substances 0.000 abstract description 3
- 241001411320 Eriogonum inflatum Species 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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Abstract
本发明提供一种培养瓶换气装置，可使培养瓶内的进气速率可控，以保证培养液的气体浓度，而且可以防止杂菌污染。该培养瓶换气装置包括培养瓶本体，还包括培养瓶本体的瓶口设置的密封垫片和用于压紧所述密封垫片的中部通孔的瓶盖，密封垫片穿设有换气管和进气管，换气管的上部与膜片式过滤器连接，进气管的下部出口接近培养瓶本体的底部。本发明的换气管上部与膜片式过滤器连接，由于膜片式过滤器的气孔分布均匀，可防止杂菌污染，而进气管的下部出口接近培养瓶本体的底部，进气时，进气管直接插入培养液，使得培养瓶的进气速率可控，而且可以极大提高培养液的气体溶解度，从而保证培养液的气体浓度，有利于微生物的培养。
The invention provides a ventilation device for a culture bottle, which can control the air intake rate in the culture bottle, so as to ensure the gas concentration of the culture solution and prevent the contamination of miscellaneous bacteria. The culture bottle ventilation device includes a culture bottle body, a sealing gasket provided on the mouth of the culture bottle body and a bottle cap for pressing the middle through hole of the sealing gasket, and the sealing gasket is pierced with a ventilation tube And the air inlet pipe, the upper part of the air exchange pipe is connected with the membrane filter, and the lower outlet of the air inlet pipe is close to the bottom of the culture bottle body. The upper part of the ventilation tube of the present invention is connected with the membrane filter, because the pores of the membrane filter are evenly distributed, which can prevent the contamination of bacteria, and the bottom outlet of the air inlet pipe is close to the bottom of the culture bottle body. Directly inserting the culture solution makes the air intake rate of the culture bottle controllable, and can greatly improve the gas solubility of the culture solution, thereby ensuring the gas concentration of the culture solution, which is beneficial to the cultivation of microorganisms.
Description
技术领域technical field
本发明涉及生物工程器具领域，具体涉及培养瓶换气装置。The invention relates to the field of bioengineering appliances, in particular to a ventilation device for a culture bottle.
背景技术Background technique
多通道菌种筛选与优化系统基于生物恒温培养箱为原型，可以对每个培养瓶进行pH测量和控制和外界营养液的添加，适用于微生物、动植物细胞菌种筛选、种子扩大、培养及其培养基的优化等方面。现有的生物恒温培养箱内，培养瓶的换气都是采用例如棉花塞等的普通瓶塞，导致培养瓶内的空气与外界交换速率较慢，而且培养液气体溶解度较低，不利于微生物的培养；此外，普通瓶塞的气孔分布不均匀，且气孔大小不可控制，容易受杂菌污染。The multi-channel strain screening and optimization system is based on a biological constant temperature incubator as a prototype. It can measure and control the pH of each culture bottle and add external nutrient solution. It is suitable for strain screening of microorganisms, animal and plant cells, seed expansion, cultivation and The optimization of its medium and so on. In the existing biological constant temperature incubator, the ventilation of the culture bottle is made of common bottle stoppers such as cotton plugs, resulting in a slow exchange rate between the air in the culture bottle and the outside world, and the low solubility of the gas in the culture solution, which is not conducive to microorganisms. In addition, the stomata of ordinary bottle stoppers are unevenly distributed, and the size of the stomata is uncontrollable, which is easily contaminated by bacteria.
发明内容Contents of the invention
本发明的目的是提出一种培养瓶换气装置，可使培养瓶内的进气速率可控，以保证培养液的气体浓度，而且可以防止杂菌污染。The object of the present invention is to propose a ventilation device for a culture bottle, which can control the air intake rate in the culture bottle to ensure the gas concentration of the culture solution and prevent contamination by bacteria.
根据本发明提供的培养瓶换气装置，包括培养瓶本体，关键在于还包括培养瓶本体的瓶口设置的密封垫片和用于压紧所述密封垫片的中部通孔的瓶盖，所述密封垫片穿设有换气管和进气管，所述换气管的上部与膜片式过滤器连接，所述进气管的下部出口接近所述培养瓶本体的底部。According to the culture bottle ventilation device provided by the present invention, it includes a culture bottle body, and the key is to also include a sealing gasket provided at the mouth of the culture bottle body and a bottle cap for pressing the middle through hole of the sealing gasket, so The sealing gasket is pierced with a ventilation tube and an air intake tube, the upper part of the ventilation tube is connected with a membrane filter, and the outlet of the lower part of the air intake tube is close to the bottom of the culture bottle body.
本发明的培养瓶换气装置，通过密封垫片保持培养瓶的瓶内与外界的空气隔绝，由穿过密封垫片的换气管和进气管分别负责培养瓶内的换气和进气，换气管上部与膜片式过滤器连接，由于膜片式过滤器的气孔分布均匀，可防止杂菌污染，而进气管的下部出口接近培养瓶本体的底部，进气时，进气管直接插入培养液，使得培养瓶的进气速率可控，而且可以极大提高培养液的气体溶解度，从而保证培养液的气体浓度，有利于微生物的培养。The ventilation device for the culture bottle of the present invention keeps the inside of the culture bottle isolated from the outside air through the sealing gasket, and the ventilation pipe and the air inlet pipe passing through the sealing gasket are responsible for the ventilation and air intake in the culture bottle respectively. The upper part of the trachea is connected to the membrane filter. Because the pores of the membrane filter are evenly distributed, it can prevent the contamination of bacteria. The lower outlet of the air inlet pipe is close to the bottom of the culture bottle body. When air is injected, the air inlet pipe is directly inserted into the culture medium , so that the air intake rate of the culture bottle can be controlled, and the gas solubility of the culture solution can be greatly improved, so as to ensure the gas concentration of the culture solution, which is beneficial to the cultivation of microorganisms.
进一步的，所述瓶盖与所述培养瓶本体的瓶口通过螺纹连接。通过螺纹连接瓶盖与瓶口，可以保证压紧密封垫片，即使密封垫片老化的情况下也足以保证瓶口的密封，而且螺纹连接方便安装和拆卸。Further, the bottle cap is threadedly connected to the mouth of the culture bottle body. By screwing the bottle cap and the bottle mouth, the sealing gasket can be guaranteed to be pressed tightly, even if the sealing gasket is aging, it is enough to ensure the sealing of the bottle mouth, and the threaded connection is convenient for installation and disassembly.
具体的说，所述培养瓶本体为锥形瓶，所述培养瓶本体的瓶身侧壁分别设有用于调节培养液酸碱度的补料口和用于安装pH传感器的开口。Specifically, the culture bottle body is an Erlenmeyer flask, and the side walls of the culture bottle body are respectively provided with a feeding port for adjusting the pH of the culture solution and an opening for installing a pH sensor.
附图说明Description of drawings
图1为本发明的结构示意图。Fig. 1 is a structural schematic diagram of the present invention.
具体实施方式detailed description
下面对照附图，通过对实施实例的描述，对本发明的具体实施方式如所涉及的各构件的形状、构造、各部分之间的相互位置及连接关系、各部分的作用及工作原理等作进一步的详细说明。Below with reference to the accompanying drawings, through the description of the implementation examples, the specific embodiments of the present invention, such as the shape, structure, mutual position and connection relationship between each part, the role and working principle of each part, etc., will be further described. detailed description of the .
如图1，本发明的培养瓶换气装置，包括培养瓶本体1，还包括培养瓶本体1的瓶口设置的密封垫片2和用于压紧密封垫片2的中部通孔的瓶盖3，密封垫片3穿设有换气管4和进气管5，换气管4的上部与膜片式过滤器6连接，进气管5的下部出口接近培养瓶本体1的底部。作为优选的实施方式，瓶盖3与培养瓶本体1的瓶口通过螺纹连接。通过螺纹连接瓶盖3与瓶口，可以保证压紧密封垫片2，即使密封垫片2老化的情况下也足以保证瓶口的密封，而且螺纹连接方便安装和拆卸。培养瓶本体1为锥形瓶，培养瓶本体1的瓶身侧壁分别设有用于调节培养液酸碱度的补料口11和用于安装pH传感器的开口12。As shown in Fig. 1, the culture bottle ventilation device of the present invention includes a culture bottle body 1, a sealing gasket 2 provided at the mouth of the culture bottle body 1 and a bottle cap for pressing the middle through hole of the sealing gasket 2 3. The sealing gasket 3 is pierced with a ventilation tube 4 and an intake tube 5 , the upper part of the ventilation tube 4 is connected to the membrane filter 6 , and the lower outlet of the gas intake tube 5 is close to the bottom of the culture bottle body 1 . As a preferred embodiment, the bottle cap 3 is connected to the bottle mouth of the culture bottle body 1 through threads. By threading the bottle cap 3 and the bottle mouth, it is possible to ensure that the sealing gasket 2 is pressed tightly, even if the sealing gasket 2 is aging, it is enough to ensure the sealing of the bottle mouth, and the threaded connection is convenient for installation and disassembly. The culture bottle body 1 is an Erlenmeyer flask, and the side wall of the culture bottle body 1 is respectively provided with a feeding port 11 for adjusting the pH of the culture solution and an opening 12 for installing a pH sensor.
本发明的培养瓶换气装置，通过密封垫片2保持培养瓶1的瓶内与外界的空气隔绝，由穿过密封垫片2的换气管4和进气管5分别负责培养瓶内的换气和进气，换气管4上部与膜片式过滤器6连接，由于膜片式过滤器6的气孔分布均匀，可防止杂菌污染，而进气管5的下部出口接近培养瓶本体1的底部，进气时，进气管5直接插入培养液，使得培养瓶1的进气速率可控，而且可以极大提高培养液的气体溶解度，从而保证培养液的气体浓度，有利于微生物的培养。The ventilation device for the culture bottle of the present invention keeps the inside of the culture bottle 1 isolated from the outside air through the sealing gasket 2, and the ventilation pipe 4 and the air inlet pipe 5 passing through the sealing gasket 2 are responsible for the ventilation in the culture bottle respectively. and air intake, the upper part of the ventilation pipe 4 is connected with the membrane filter 6, because the pores of the membrane filter 6 are evenly distributed, which can prevent the contamination of bacteria, and the lower outlet of the air inlet pipe 5 is close to the bottom of the culture bottle body 1, During air intake, the intake pipe 5 is directly inserted into the culture solution, so that the intake rate of the culture bottle 1 is controllable, and the gas solubility of the culture solution can be greatly improved, thereby ensuring the gas concentration of the culture solution, which is beneficial to the cultivation of microorganisms.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610945100.0A CN106434314A (en) | 2016-11-02 | 2016-11-02 | Culture bottle air change device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610945100.0A CN106434314A (en) | 2016-11-02 | 2016-11-02 | Culture bottle air change device |
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| CN106434314A true CN106434314A (en) | 2017-02-22 |
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| CN201610945100.0A Pending CN106434314A (en) | 2016-11-02 | 2016-11-02 | Culture bottle air change device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109392684A (en) * | 2018-10-24 | 2019-03-01 | 杭州师范大学 | Wetland plant clone organ Growth trends observation device and application method |
| CN112903830A (en) * | 2019-11-19 | 2021-06-04 | 上海交通大学 | Test device and method for measuring oxidation rate of methane in rice field soil |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2786131Y (en) * | 2005-05-25 | 2006-06-07 | 李俊贤 | Liquid strain incubator |
| CN104862222A (en) * | 2015-05-05 | 2015-08-26 | 大连大学 | Microorganism fermentation device |
| CN205662523U (en) * | 2016-06-06 | 2016-10-26 | 东北林业大学 | Plant cell cultivates teaching and uses plain type bioreactor |
| CN206266605U (en) * | 2016-11-02 | 2017-06-20 | 广东石油化工学院 | A kind of blake bottle air interchanger |
-
2016
- 2016-11-02 CN CN201610945100.0A patent/CN106434314A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2786131Y (en) * | 2005-05-25 | 2006-06-07 | 李俊贤 | Liquid strain incubator |
| CN104862222A (en) * | 2015-05-05 | 2015-08-26 | 大连大学 | Microorganism fermentation device |
| CN205662523U (en) * | 2016-06-06 | 2016-10-26 | 东北林业大学 | Plant cell cultivates teaching and uses plain type bioreactor |
| CN206266605U (en) * | 2016-11-02 | 2017-06-20 | 广东石油化工学院 | A kind of blake bottle air interchanger |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109392684A (en) * | 2018-10-24 | 2019-03-01 | 杭州师范大学 | Wetland plant clone organ Growth trends observation device and application method |
| CN109392684B (en) * | 2018-10-24 | 2024-01-09 | 杭州师范大学 | Dynamic observation device for growth of wetland plant clone organ and use method thereof |
| CN112903830A (en) * | 2019-11-19 | 2021-06-04 | 上海交通大学 | Test device and method for measuring oxidation rate of methane in rice field soil |
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