CN206457493U - A kind of pocket type anoxic box - Google Patents
A kind of pocket type anoxic box Download PDFInfo
- Publication number
- CN206457493U CN206457493U CN201720142384.XU CN201720142384U CN206457493U CN 206457493 U CN206457493 U CN 206457493U CN 201720142384 U CN201720142384 U CN 201720142384U CN 206457493 U CN206457493 U CN 206457493U
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- China
- Prior art keywords
- diaphragm seal
- bottom plate
- fixed
- suction nozzle
- pocket type
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Abstract
The utility model provides a kind of pocket type anoxic box, belongs to cell biological experimental technique field.It solves the technical problems such as existing anoxic box gas displacement time length.This pocket type anoxic box, including bottom plate, it is characterized in that, support frame is fixed with the bottom plate, bottom is arranged with outside the support frame has the diaphragm seal of opening, the top surface removable seal of the bottom opening of the diaphragm seal and the bottom plate is fixed, top surface one sealed volume of formation of the diaphragm seal and bottom plate, the diaphragm seal is fixed with a suction nozzle, the diaphragm seal is further fixed on an exhaust nozzle, the suction nozzle and exhaust nozzle are connected with described sealed volume, intake valve is provided with the suction nozzle, air bleeding valve is provided with the exhaust nozzle, Tissue Culture Dish placement rack is fixed with bottom plate in the sealed volume, oxygen content detector is fixed with bottom plate in the sealed volume.The utility model has the advantages that row's scavenging efficiency is high, it is short to set up anaerobic environment required time.
Description
Technical field
The utility model belongs to cell biological experimental technique field, is related to a kind of anoxic box, particularly a kind of pocket type anoxic
Box.
Background technology
Anoxic box is used to set up hypoxic cell model, and existing equipment is:(1) three gas incubators are may be programmed, the device can lead to
Cross electronic program and accurately control oxygen, nitrogen, the air inlet proportion of three kinds of gases of carbon dioxide, with the ratio of three's gas in regulation box
Example, the need for meeting various different anoxics experiments, but the equipment exist it is expensive, take that lab space is larger, safeguards and protects
The shortcomings of supporting numerous and diverse, and a kind of fixed gas ratio can only be maintained in incubator in the same period, it is impossible to meet and carry out difference simultaneously
The anoxic requirement of experiment of gas content；(2) anoxic box, is also the excellent selection for setting up hypoxic cell model, and the device is one
Channel inlet, channel vent can closed gas mixing apparatus, by being continually fed into nitrogen and carbon dioxide gas mixture
(or being passed through pure carbon dioxide and nitrogen, cross control the two velocity ratio needs the mixed gas of ratio to produce) is to replace in case
Air, finally close into outlet passageway, to obtain anaerobic environment.It is simple convenient that the device advantage is, can be same as needed
When the anaerobic environment of gas with various ratio is set up in multiple anoxic boxes, have the disadvantage that the gas displacement time is longer, cell during ventilation
It need to be exposed to for a long time<37 degrees Celsius of environment, and final oxygen content can not be accurately detected, also there is gas and waste serious etc. lack
Point.
The content of the invention
The purpose of this utility model is there is above mentioned problem for existing technology, it is proposed that a kind of pocket type anoxic box, is carried
The row's of liter scavenging efficiency, the time required to anaerobic environment is set up in reduction.
The purpose of this utility model can be realized by following technical proposal：A kind of pocket type anoxic box, including bottom plate, it is special
Levy and be, be fixed with the bottom plate outside support frame, the support frame and be arranged with bottom there is the diaphragm seal of opening, it is described
The top surface removable seal of the bottom opening of diaphragm seal and the bottom plate is fixed, and the top surface formation one of the diaphragm seal and bottom plate is close
Cavity volume is sealed, the diaphragm seal is fixed with a suction nozzle, and the diaphragm seal is further fixed on an exhaust nozzle, the suction nozzle and exhaust nozzle
Connected with described sealed volume, be provided with the suction nozzle in intake valve, the exhaust nozzle and be provided with air bleeding valve, it is described
Oxygen content inspection is fixed with the bottom plate being fixed with bottom plate in sealed volume in Tissue Culture Dish placement rack, the sealed volume
Survey device.
Its operation principle is as follows：The opening of diaphragm seal bottom and the top surface of bottom plate are separated, diaphragm seal is started, first will sealing
Film is tried one's best be collapsed, the volume of diaphragm seal cavity volume is tried one's best reduction, then Tissue Culture Dish is placed on placement rack, then by diaphragm seal bottom
Portion's edge of opening is fixed with plate top surface, is formed closing cavity volume, is then shut off intake valve, exhaust nozzle is passed through into connecting tube and experiment
The aspiration pump connection of room, opens air bleeding valve, the air that diaphragm seal is formed in cavity volume is detached by aspiration pump, sealing during this
Film shrinks, support frame support diaphragm seal, prevents diaphragm seal from caving in completely and causes to contact with Tissue Culture Dish, diaphragm seal cavity volume
Inside detach after partial air, close air bleeding valve, open intake valve, by laboratory carbon dioxide and nitrogen conveying equipment toward air inlet
Mouth is passed through carbon dioxide and nitrogen mixed gas, diaphragm seal is filled again；Point out situation regulation close according to oxygen content detector
Sealer holds intracavity gas, and such as oxygen content is higher to open air bleeding valve, continues to be passed through carbon dioxide toward suction nozzle and mix with nitrogen
Gas, exhaust nozzle is persistently vented, and reduces oxygen content, such as oxygen content is too low, then can be suitably toward being passed through oxygen, lifting oxygen in suction nozzle
Content, untill the oxygen content in diaphragm seal cavity volume reaches requirement of experiment, turns off intake valve and air bleeding valve, this pocket type is lacked
Oxygen box is put into 37 degrees Celsius of isoperibols and carries out incubated experiment.
Above-mentioned support frame includes some vertical rods, and the vertical rod is perpendicularly fixed at described plate top surface, described adjacent
Cross bar is connected between the top of vertical rod, some vertical rods and cross bar is constituted a cuboid framework, described diaphragm seal is set in
A cavity volume for being in cuboid is formed on described framework.The cuboid framework that some vertical rods and cross bar are constituted plays support diaphragm seal
Effect, make diaphragm seal formation sealed volume, while prevent diaphragm seal cavity volume be evacuated when diaphragm seal cave in and and Tissue Culture Dish
Contact.
The top surface of above-mentioned bottom plate offers slot, and head and the tail are connected in a ring for periphery of the slot along some columns,
Detachable interposing is provided with a pressing plate in a ring in the slot, and the bottom of the pressing plate has what is matched with the slot shape
Raised line.By the edges cover of diaphragm seal bottom opening on slot, then by the raised line insertion slot of pressing plate bottom, make diaphragm seal
Bottom opening and the top surface of bottom plate fix so that top surface one sealed volume of formation of diaphragm seal and bottom plate, when needing to take apart
When diaphragm seal takes out Tissue Culture Dish, pressing plate is levered up, the raised line of pressing plate bottom is separated out of slot, makes opening for diaphragm seal bottom
Mouth edge is separated with the top surface of bottom plate.
Above-mentioned suction nozzle and exhaust nozzle are fixed with the diaphragm seal, and the side of the cuboid cavity volume positioned at the formation.
Suction nozzle and exhaust nozzle have certain weight, and diaphragm seal is relatively thin, suction nozzle and exhaust nozzle are lifted by cross bar, in diaphragm seal
When cavity volume is evacuated, suction nozzle and exhaust nozzle is prevented to be located at the top of diaphragm seal and make the excessive be collapsed of diaphragm seal.
Above-mentioned suction nozzle is located at horizontal one end of the cuboid cavity volume side, and the exhaust nozzle holds positioned at the cuboid
The horizontal other end of chamber side.Try one's best and separate the purpose is to the distance for making suction nozzle and exhaust nozzle, prevent air inlet and exhaust interference,
Improve the efficiency of ventilation in diaphragm seal cavity volume.
Above-mentioned diaphragm seal is transparent plastic film.It is easy to observe the situation of Tissue Culture Dish inner cell Bioexperiment.
Compared with prior art, the present apparatus is simple and easy to operate, before Tissue Culture Dish is placed toward on base, first will sealing
Film is tried one's best be collapsed, the volume of diaphragm seal cavity volume is tried one's best reduction, then extracts the air in diaphragm seal cavity volume by aspiration pump, greatly
Lifting row's scavenging efficiency, is greatly reduced the time required to setting up anaerobic environment, while avoiding cell from being exposed to too long<37 degrees Celsius
Oxygen content in the uncontrollable influence that environment is produced on experiment, oxygen content detector detection diaphragm seal cavity volume, improves experimental ring
The accurate foundation in border.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation of bottom plate in the utility model.
Fig. 3 is the partial structurtes enlarged drawing of bottom plate in the utility model.
Fig. 4 is the structural representation of the utility model center platen.
Fig. 5 is that the utility model center platen looks up partial structurtes enlarged drawing.
In figure, 1, bottom plate；1a, slot；2nd, support frame；2a, vertical rod；2b, cross bar；3rd, diaphragm seal；4th, suction nozzle；5th, arrange
Valve；6th, intake valve；7th, air bleeding valve；8th, placement rack；9th, oxygen content detector；10th, pressing plate；10a, raised line.
Embodiment
The following is specific embodiment of the utility model and with reference to accompanying drawing, further is made to the technical solution of the utility model
Description, but the utility model is not limited to these embodiments.
As shown in figure 1, a kind of pocket type anoxic box, including bottom plate 1, it is characterised in that support frame 2 is fixed with bottom plate 1,
Being arranged with bottom outside support frame 2 has the diaphragm seal 3 of opening, and the bottom opening of diaphragm seal 3 and the top surface of bottom plate 1 are detachably close
Sealing is determined, and top surface one sealed volume of formation of diaphragm seal 3 and bottom plate 1, diaphragm seal 3 is fixed with a suction nozzle 4, and diaphragm seal 3 is also solid
Surely there is an exhaust nozzle 5, suction nozzle 4 and exhaust nozzle 5 are connected with sealed volume, and intake valve 6, exhaust nozzle 5 are provided with suction nozzle 4
The bottom plate 1 in Tissue Culture Dish placement rack 8, sealed volume is fixed with the bottom plate 1 being inside provided with air bleeding valve 7, sealed volume
On be fixed with oxygen content detector 9, the oxygen content detector be prior art products.
The quantity that support frame 2 includes vertical rod 2a in some vertical rod 2a, the present embodiment is four, and vertical rod 2a is perpendicularly fixed at
The top surface of bottom plate 1, makes four vertical rod 2a bottom fixed position be formed between rectangular four angle points, adjacent vertical rod 2a top
Cross bar 2b is connected with, some vertical rod 2a and cross bar 2b is constituted a cuboid framework, diaphragm seal 3 is set in formation one on framework and is in
The cavity volume of cuboid.
As shown in Fig. 2, Fig. 3, Fig. 4 and Fig. 5, the top surface of bottom plate 1 offers slot 1a, peripheries of the slot 1a along some columns
Head and the tail are connected in a ring, and detachable interposing is provided with a pressing plate 10 in a ring in slot 1a, and the bottom of pressing plate 10 has and slot 1a
The raised line 10a that shape matches.
Suction nozzle 4 and exhaust nozzle 5 are fixed with diaphragm seal 3, and the side of the cuboid cavity volume positioned at the formation.
Suction nozzle 4 is located at horizontal one end of the cuboid cavity volume side, and exhaust nozzle 5 is located at cuboid cavity volume side
The horizontal other end.
Diaphragm seal 3 is transparent plastic film.
Specific embodiment described herein is only to the utility model spirit explanation for example.The utility model institute
Category those skilled in the art can make various modifications or supplement or using similar to described specific embodiment
Mode substitute, but without departing from spirit of the present utility model or surmount scope defined in appended claims.
Although more having used 1, bottom plate herein；1a, slot；2nd, support frame；2a, vertical rod；2b, cross bar；3rd, seal
Film；4th, suction nozzle；5th, exhaust nozzle；6th, intake valve；7th, air bleeding valve；8th, placement rack；9th, oxygen content detector；10th, pressing plate；It is 10a, convex
The terms such as bar, but it is not precluded from the possibility using other terms.Using these terms just for the sake of more easily description and
Explain essence of the present utility model；Being construed as any additional limitation is disagreed with the utility model spirit
's.
Claims (6)
1. a kind of pocket type anoxic box, including bottom plate (1), it is characterised in that be fixed with support frame (2), institute on the bottom plate (1)
State and bottom is arranged with outside support frame (2) there is the diaphragm seal (3) of opening, bottom opening and the bottom of the diaphragm seal (3)
The top surface removable seal of plate (1) is fixed, the diaphragm seal (3) and top surface one sealed volume of formation of bottom plate (1), the sealing
Film (3) is fixed with a suction nozzle (4), and the diaphragm seal (3) is further fixed on an exhaust nozzle (5), the suction nozzle (4) and exhaust nozzle
(5) connected with described sealed volume, be provided with intake valve (6), the exhaust nozzle (5) and set in the suction nozzle (4)
Have and be fixed with the bottom plate (1) in air bleeding valve (7), the sealed volume in Tissue Culture Dish placement rack (8), the sealed volume
Bottom plate (1) on be fixed with oxygen content detector (9).
2. a kind of pocket type anoxic box according to claim 1, it is characterised in that described support frame (2) includes some
Vertical rod (2a), the vertical rod (2a), which is perpendicularly fixed between described bottom plate (1) top surface, the top of the adjacent vertical rod (2a), to be connected
Cross bar (2b) is connected to, some vertical rods (2a) and cross bar (2b) is constituted a cuboid framework, described diaphragm seal (3) is set in institute
A cavity volume for being in cuboid is formed on the framework stated.
3. a kind of pocket type anoxic box according to claim 1 or 2, it is characterised in that the top surface of the bottom plate (1) is offered
Slot (1a), head and the tail are connected in a ring for periphery of the slot (1a) along some columns, detachable in the slot (1a)
A pressing plate (10) in a ring is inserted with, the bottom of the pressing plate (10) has the raised line matched with the slot (1a) shape
(10a)。
4. a kind of pocket type anoxic box according to claim 2, it is characterised in that the suction nozzle (4) and exhaust nozzle (5) with
The diaphragm seal (3) is fixed, and the side of the cuboid cavity volume positioned at the formation.
5. a kind of pocket type anoxic box according to claim 4, it is characterised in that the suction nozzle (4) is located at described rectangular
Horizontal one end of body cavity volume side, the exhaust nozzle (5) is located at the horizontal other end of the cuboid cavity volume side.
6. a kind of pocket type anoxic box according to claim 1, it is characterised in that described diaphragm seal (3) is transparent plastic
Film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720142384.XU CN206457493U (en) | 2017-02-16 | 2017-02-16 | A kind of pocket type anoxic box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720142384.XU CN206457493U (en) | 2017-02-16 | 2017-02-16 | A kind of pocket type anoxic box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206457493U true CN206457493U (en) | 2017-09-01 |
Family
ID=59695107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720142384.XU Expired - Fee Related CN206457493U (en) | 2017-02-16 | 2017-02-16 | A kind of pocket type anoxic box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206457493U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113604332A (en) * | 2021-07-20 | 2021-11-05 | 西南医科大学附属中医医院 | Disposable oxygen-deficient environment cell culture bag |
-
2017
- 2017-02-16 CN CN201720142384.XU patent/CN206457493U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113604332A (en) * | 2021-07-20 | 2021-11-05 | 西南医科大学附属中医医院 | Disposable oxygen-deficient environment cell culture bag |
| CN113604332B (en) * | 2021-07-20 | 2023-09-19 | 西南医科大学附属中医医院 | Disposable anaerobic environment cell culture bag |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170901 Termination date: 20190216 |