CN113604332A

CN113604332A - Disposable oxygen-deficient environment cell culture bag

Info

Publication number: CN113604332A
Application number: CN202110818275.6A
Authority: CN
Inventors: 刘孟楠; 杨思进; 刘天助; 任维; 罗钢; 刘平; 刘佳利; 杨廷富; 刘颜
Original assignee: Affiliated Hospital of Traditional Chinese Medicine TCM of Southwest Medical University
Current assignee: Affiliated Hospital of Traditional Chinese Medicine TCM of Southwest Medical University
Priority date: 2021-07-20
Filing date: 2021-07-20
Publication date: 2021-11-05
Anticipated expiration: 2041-07-20
Also published as: CN113604332B

Abstract

The invention discloses a disposable anoxic environment cell culture bag, which comprises a base and a cover body separately arranged on the upper side of the base, wherein the cover body is made of disposable soft rubber material, the cover body and the base are fixedly connected through a clamp, the lower side of the base is concave, round bosses penetrating through the bottom are arranged at the center and the periphery of the bottom, and through holes are arranged in the round bosses. According to the invention, the film is laid on the base to be contacted with the inner edge platform on the cover body, and the disposable soft adhesive of the cover body is arranged, so that a double sealing effect is formed when the base and the cover body are inflated, the high-temperature sterilization operation of the cover body is avoided, the pollution risk is reduced, and the operation process is simplified; the first filter tip communicated through the first communication pipe inflates air into the cavity in the middle part, and then the cover body is jacked up, and meanwhile, the air is discharged through the second filter tip on the periphery, so that the inflating process is visual and controllable, and the operation is convenient.

Description

Disposable oxygen-deficient environment cell culture bag

Technical Field

The invention relates to the field of test instruments, in particular to a disposable anoxic environment cell culture bag.

Background

The anoxic incubator is a self-sufficient sealed chamber suitable for the existing laboratory incubator. Each chamber is sealed independently with a portion of the O-ring compressed uniformly by the ring clamp to form a reliable airtight chamber. The cylindrical wall and hemispherical top and bottom minimize air flow resistance and eliminate dead zones during purging. Aerify through gas cylinder and supporting flowmeter when using can, then put in normal incubator, but present lid is made for the molding, need carry out high temperature disinfection after the use, uses repeatedly, so causes the risk of pollution easily, and air inlet and gas outlet all are in the bottom when aerifing in addition, and the inconvenient volume of aerifing that carries out confirms when gas exchange causes sealed space gas content not well controlled.

Disclosure of Invention

The present invention aims to solve the above problems by providing a disposable oxygen-deficient cell culture bag.

The invention realizes the purpose through the following technical scheme:

disposable oxygen deficiency environment cell culture bag, including the base, the separation sets up the lid of base upside, the lid is disposable soft adhesive material, the lid with it is fixed to connect through the clamp between the base, the concavity is personally submitted to the base downside, and bottom surface center and border position are provided with the circular boss that passes this bottom surface, the inside through-hole that runs through that is provided with of this circular boss, the base center circular boss upper end is provided with first filter tip, lower extreme and is connected with first communicating pipe, the base border circular boss upper end is provided with the second filter tip, the lower extreme is provided with the second communicating pipe, first communicating pipe all be provided with the pipe clamp on the second communicating pipe external diameter, the base with be provided with the sealing washer between the contact surface about the lid, be provided with disposable film on the upper surface of base.

Further setting: the base is circular, and upper end external diameter periphery is provided with the flange, be provided with first recess on the flange upper surface.

So set up, make the base supports the household utensils box and places, first recess is right the sealing washer carries out spacing fixed.

Further setting: the side of the cover body is hemispherical, an inner edge platform is arranged on the inner side of the lower end face of the cover body, and an outer edge platform is arranged on the lower side of the outer surface of the cover body.

So set up, make outer edge platform inner edge platform respectively with the film flange forms sealedly, is convenient for first connecting pipe lets in gas the lid with in the sealed cavity behind the base lock, gas from the top to spread all around, will the lid props up.

Further setting: the clamp cross-section is concave type form, the clamp with outer fringe platform on the lid flange interference on the base is connected.

So set up, make the clamp be convenient for with the base the lid lock joint together, form sealed cavity.

Further setting: the sealing ring is in interference connection with the first groove of the upper flange of the base and the upper outer edge platform of the cover body.

So set up, through the sealing washer is right the base, carry out sealing connection between the lid.

Further setting: the circular boss is in the base periphery is provided with everywhere, first filter tip second filter tip respectively with circular boss upper end threaded connection, first filter tip second filter tip inboard is provided with the filter screen.

The arrangement facilitates the connection and fixation of the first filter tip and the second filter tip on the circular boss, and simultaneously filters gas in the process of gas inlet and outlet.

Further setting: the film is laid on the upper end face of the base, and through holes through which the first filter tip and the second filter tip pass are formed in the film.

So set up, through laying of film improves the base with the leakproofness of the inner fringe platform of lid makes the base carry out the diaphragm simultaneously with the household utensils box of inner chamber and handles, avoids the pollution in the cavity.

Further setting: the second communicating pipe is positioned at the lower end of the base and is annular, and a straight pipe connected with the circular boss at the lower bottom surface of the base is arranged at the upper end of the second communicating pipe.

So set up, be convenient for the second communicating pipe with the base week circular boss intercommunication carries out business turn over gas operation to seal chamber.

Further setting: and a vessel box is arranged between the circular bosses on the upper end surface of the base.

So set up, place the cell that needs to cultivate in the household utensils box improves operation space's utilization ratio.

Further setting: the first filter is at a height near the top of the cap, the first filter is at a height greater than the second filter, and the outer diameters of the film and the cap are greater than the outer diameter of the base.

So set up, make behind the lid sealed accessible the second filter tip or first filter tip gets into the cavity to different gas and selects, and can fill the cavity fast, makes the film the lid outer fringe passes through the clamp is sealed both.

Compared with the prior art, the invention has the following beneficial effects:

the film is laid on the base to be in contact with the inner edge platform on the cover body, and the disposable soft adhesive of the cover body is arranged, so that a double sealing effect is formed when the base and the cover body are inflated, the high-temperature sterilization operation of the cover body is avoided, the risk of infection is reduced, and the operation process is simplified; the first filter tip communicated through the first communication pipe inflates air into the cavity in the middle part, and then the cover body is jacked up, and meanwhile, the air is discharged through the second filter tip on the periphery, so that the inflating process is visual and controllable, and the operation is convenient.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic view of a first configuration of a disposable anoxic environmental cell culture bag according to the present invention;

FIG. 2 is a second schematic diagram of a disposable anoxic environmental cell culture bag according to the invention;

FIG. 3 is a first schematic structural view of an exploded structure of the disposable anoxic environmental cell culture bag according to the present invention;

FIG. 4 is a second schematic diagram of an exploded configuration of the disposable hypoxic environment cell culture bag according to the invention;

FIG. 5 is a schematic top view of the disposable anoxic environmental cell culture bag according to the present invention;

FIG. 6 is a schematic sectional view A-A of FIG. 5;

FIG. 7 is an enlarged partial schematic view of FIG. 6;

FIG. 8 is a schematic diagram of the construction of a disposable anoxic environmental cell culture bag according to the invention;

FIG. 9 is a schematic structural diagram of the disposable anoxic environmental cell culture bag according to the present invention in a state where the capsule is placed.

The reference numerals are explained below:

1. a base; 101. a flange; 102. a first groove; 2. a cover body; 201. an inner edge platform; 202. a peripheral table; 3. clamping a hoop; 4. a first communication pipe; 5. a second communicating pipe; 6. a pipe clamp; 7. a seal ring; 8. a film; 9. a first filter; 10. a second filter; 11. a circular boss; 12. a capsule.

Detailed Description

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The invention will be further described with reference to the accompanying drawings in which:

as shown in figures 1-9, the disposable anoxic environment cell culture bag comprises a base 1, a cover body 2 separately arranged on the upper side of the base 1, the cover body 2 is a disposable soft rubber material, the cover body 2 is fixedly connected with the base 1 through a clamp 3, the lower side of the base 1 is concave, a circular boss 11 penetrating through the bottom is arranged at the center and the periphery of the bottom, a through hole is arranged inside the circular boss 11 for facilitating the connection and fixation with a first communicating pipe 4, a second communicating pipe 5, a first filter tip 9 and a second filter tip 10, the upper end of the circular boss 11 at the center of the base 1 is provided with the first filter tip 9, the lower end is connected with the first communicating pipe 4, the upper end of the circular boss 11 at the periphery of the base 1 is provided with the second filter tip 10, the lower end is provided with the second communicating pipe 5, the first communicating pipe 4 and the second communicating pipe 5 are both provided with a pipe clamp 6 on the outer diameter, a sealing ring 7 is arranged between the upper contact surface and the lower contact surface of the base 1 and the cover body 2, and a disposable film 8 is arranged on the upper surface of the base 1.

Preferably: the base 1 is circular, a flange 101 is arranged on the outer diameter circumference of the upper end of the base 1, a first groove 102 is formed in the upper surface of the flange 101, the base 1 supports the vessel box 12, and the first groove 102 limits and fixes the sealing ring 7; the upper side of the cover body 2 is hemispherical, the inner edge table 201 is arranged on the inner side of the lower end face of the cover body 2, the outer edge table 202 is arranged on the lower side of the outer surface of the cover body 2, so that the outer edge table 202 and the inner edge table 201 respectively form sealing with the film 8 and the flange 101, the first communication pipe 4 is convenient for introducing gas into a sealed cavity formed by buckling the cover body 2 and the base 1, and the gas diffuses from the top to the periphery to support the cover body 2; the section of the hoop 3 is concave, and the hoop 3 is in interference connection with the outer edge platform 202 on the cover body 2 and the flange 101 on the base 1, so that the hoop 3 is convenient for buckling the base 1 and the cover body 2 together to form a sealed cavity; the sealing ring 7 is in interference connection with the first groove 102 of the upper flange 101 of the base 1 and the upper outer edge platform 202 of the cover body 2, and the base 1 and the cover body 2 are in sealing connection through the sealing ring 7; the circular boss 11 is arranged at four positions around the base 1, the first filter tip 9 and the second filter tip 10 are respectively in threaded connection with the upper end of the circular boss 11, and filter screens are arranged on the inner sides of the first filter tip 9 and the second filter tip 10, so that the first filter tip 9 and the second filter tip 10 can be conveniently connected and fixed on the circular boss 11, and meanwhile, air is filtered when air enters or exits; the film 8 is laid on the upper end face of the base 1, through holes through which the first filter tip 9 and the second filter tip 10 penetrate are formed in the film 8, the tightness of the base 1 and the inner edge table 201 of the cover body 2 is improved through the laying of the film 8, and meanwhile the base 1 and the inner cavity vessel box 12 are subjected to membrane treatment, so that pollution in the cavity is avoided; the second communicating pipe 5 is positioned at the lower end of the base 1 and is annular, and the upper end of the second communicating pipe is provided with a straight pipe connected with the circular boss 11 on the lower bottom surface of the base 1, so that the second communicating pipe 5 is conveniently communicated with the circular boss 11 on the periphery of the base 1, and the sealed cavity is subjected to air inlet and outlet operation; the upper end surface of the base 1 is provided with three vessel boxes 12 positioned between the circular bosses 11, the inner sides of the vessel boxes 12 are provided with three layers, cells to be cultured are placed in the vessel boxes 12, and the utilization rate of an operation space is improved; the height of the first filter 9 is close to the top of the cover body 2, the height of the first filter 9 is larger than that of the second filter 10, the outer diameters of the film 8 and the cover body 2 are larger than that of the base 1, different gases can enter the cavity through the second filter 10 or the first filter 9 after the cover body 2 is sealed, the cavity can be rapidly filled, and the film 8 and the cover body 2 are sealed through the hoop 3 at the outer edge.

The working principle and the using process of the invention are as follows: laying a disposable film 8 on the upper end face of a base 1, placing a vessel box 12 with cells on the film 8, placing a cover body 2 on the upper end of the base 1, pressing an inner edge platform 201 of the cover body 2 on the film 8, buckling an outer edge platform 202 of the film 8 on a sealing ring 7 of a peripheral flange 101 of the base 1, buckling and fixing the outer edge platform 202, the flange 101 and the film 8 through a hoop 3, connecting a first communicating pipe 4 with an air inlet source, enabling the air inlet source to continuously enter a cavity formed by the base 1 and the cover body 2, continuously upwards supporting the cover body 2 at the middle part, spreading the air to the periphery along the inner wall of the cover body 2 after reaching the top of the cover body 2, discharging the air outwards through a peripheral second filter tip 10, or connecting the air inlet source through a second communicating pipe 5, enabling the air to be inflated into the cavity through the second filter tip 10, and discharged outwards through the first filter tip 9, until the cover body 2 is completely jacked up to be circular, the pipe clamp 6 is buckled.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.

Claims

1. Disposable oxygen deficiency environment cell culture bag, including base (1), its characterized in that: the cover body (2) is arranged on the upper side of the base (1) in a separated mode, the cover body (2) is made of disposable soft rubber materials, the cover body (2) and the base (1) are fixedly connected through a clamp (3), the lower side of the base (1) is concave, a circular boss (11) penetrating through the bottom surface is arranged at the center and the periphery of the bottom surface, a through hole penetrating through the circular boss (11) is formed in the inner portion of the circular boss (11), a first filter tip (9) is arranged at the upper end of the circular boss (11) at the center of the base (1), a first communicating pipe (4) is connected to the lower end of the circular boss (11), a second filter tip (10) is arranged at the upper end of the periphery of the base (1), a second communicating pipe (5) is arranged at the lower end of the circular boss (11), pipe clamps (6) are arranged on the outer diameters of the first communicating pipe (4) and the second communicating pipe (5), and a sealing ring (7) is arranged between the upper contact surface and the lower contact surface of the base (1) and the cover body (2), the upper surface of the base (1) is provided with a disposable film (8).

2. The disposable hypoxic-environment cell culture bag of claim 1, wherein: the base (1) is circular, a flange (101) is arranged on the periphery of the outer diameter of the upper end of the base, and a first groove (102) is formed in the upper surface of the flange (101).

3. The disposable hypoxic-environment cell culture bag of claim 2, wherein: the side of the cover body (2) is hemispherical, an inner edge platform (201) is arranged on the inner side of the lower end face of the cover body (2), and an outer edge platform (202) is arranged on the lower side of the outer surface of the cover body (2).

4. The disposable hypoxic-environment cell culture bag of claim 3, wherein: the cross-section of the clamp (3) is concave, and the clamp (3) is in interference connection with an outer edge table (202) on the cover body (2) and a flange (101) on the base (1).

5. The disposable hypoxic-environment cell culture bag of claim 4, wherein: the sealing ring (7) is in interference connection with a first groove (102) of an upper flange (101) of the base (1) and an upper outer edge table (202) of the cover body (2).

6. The disposable hypoxic-environment cell culture bag of claim 1, wherein: the circular boss (11) is arranged at four positions on the periphery of the base (1), the first filter tip (9) and the second filter tip (10) are respectively in threaded connection with the upper end of the circular boss (11), and a filter screen is arranged on the inner side of each of the first filter tip (9) and the second filter tip (10).

7. The disposable hypoxic-environment cell culture bag of claim 1, wherein: the film (8) is laid on the upper end face of the base (1), and through holes through which the first filter tip (9) and the second filter tip (10) pass are formed in the film (8).

8. The disposable hypoxic-environment cell culture bag of claim 1, wherein: the second communicating pipe (5) is positioned at the lower end of the base (1) and is annular, and a straight pipe connected with the circular boss (11) at the lower bottom surface of the base (1) is arranged at the upper end of the second communicating pipe.

9. The disposable hypoxic-environment cell culture bag of claim 1, wherein: and a vessel box (12) is arranged between the circular bosses (11) on the upper end surface of the base (1).

10. The disposable hypoxic-environment cell culture bag of claim 1, wherein: the first filter (9) is at a height close to the top of the cap (2), the first filter (9) is at a height greater than the second filter (10), and the membrane (8), the cap (2) have an outer diameter greater than the outer diameter of the chassis (1).