CN213506979U - Simple and easy cell oxygen deficiency culture apparatus - Google Patents
Simple and easy cell oxygen deficiency culture apparatus Download PDFInfo
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- CN213506979U CN213506979U CN202022081749.7U CN202022081749U CN213506979U CN 213506979 U CN213506979 U CN 213506979U CN 202022081749 U CN202022081749 U CN 202022081749U CN 213506979 U CN213506979 U CN 213506979U
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Abstract
The utility model belongs to the technical field of culture devices, and discloses a simple cell oxygen deficiency culture device, which comprises a box body, wherein a culture bin is arranged at the upper position inside the box body, a constant temperature bin is arranged at the lower position inside the box body, a top cover is arranged at the top of the box body and is positioned above the culture bin, a base is fixedly connected at the bottom of the box body, and supporting legs are fixedly connected at the bottom of the base, the utility model is provided with the top cover and a clamping device, so that in the research process of culturing under the cell oxygen deficiency environment, not only can the observation by naked eyes be needed, but also different conditions can be generated in the experiment process, the culture dish can be operated in a related way, the culture box can be opened in time through the top cover so as to complete the related operation on the culture dish, and the clamping device can ensure the tight fit between the top, after the top cover is closed, the airtightness of the incubator is ensured, and the efficiency of the culture work is improved.
Description
Technical Field
The utility model belongs to the technical field of culture apparatus, concretely relates to simple and easy cell oxygen deficiency culture apparatus.
Background
If people want to make more breakthrough and progress in the biological field, people need to carry out related research on hypoxic cells all the time so as to obtain the related action of related factors in the pathogenesis of diseases and further carry out related treatment on the hypoxic cells, and the research on the hypoxic cells needs to firstly carry out culture work on the hypoxic cells.
The existing cell hypoxia culture device can not conveniently and timely open the culture box and has poor sealing performance when the experiment needs to be stopped or suspended midway during the hypoxia culture work to carry out related operations on the cells in the culture dish, and does not provide a suitable living environment for the survival of the hypoxic cells and is troublesome in supplementing and replacing hypoxic gas.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a simple and easy cell oxygen deficiency culture apparatus to thereby solve current cell oxygen deficiency culture apparatus and be difficult for opening the incubator and carry out relevant adjustment or operation and the not enough problem of box leakproofness to the culture dish at the in-process of carrying out the cultivation work, do not provide suitable living condition and the gaseous problem of inconvenient supplementary oxygen deficiency type for the oxygen deficiency cell.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a simple and easy cell oxygen deficiency culture apparatus, includes the box, the inside top position of box is provided with cultivates the storehouse, the inside below position of box is provided with constant temperature storehouse, the top of box is provided with the top cap, the top cap is located the top of cultivateing the storehouse, the bottom fixedly connected with base of box, the bottom fixedly connected with landing leg of base, the landing leg is provided with four altogether.
Preferably, the outside one side of top cap rotates and is connected with the hinge, the one end of hinge is connected in the outer wall of top cap, the other end of hinge is connected in the outer wall of box, the box passes through hinge rotation with the top cap and is connected, top cap and box connection position are provided with the rubber pad, rubber pad fixed connection is in the top cap bottom.
Preferably, the top cap is provided with chucking device for the opposite side of hinge, chucking device includes the boss, boss fixed connection is in the outer wall of top cap, the boss chucking is connected with the snap ring, the handle is installed to the position that the box outer wall is close to the boss, the outside of handle is rotated and is connected with the snap ring, the one end chucking that the handle was kept away from to the snap ring is connected in the boss outside.
Preferably, the internally mounted of base has the motor, the output of motor is connected with the pivot, the one end that the motor was kept away from in the pivot is connected with the heater, be provided with the bearing between pivot and the heater, the upper end fixedly connected with heating pipe of heater, the heating pipe sets up inside the constant temperature storehouse.
Preferably, the culture bin is internally provided with a bottom plate, the two sides of the bottom plate are fixedly connected to the inner wall of the culture bin, and a culture dish is arranged above the bottom plate.
Preferably, the outside one side of box is provided with the gas bomb, the one side that the box was kept away from to the gas bomb is connected with the intake pipe, install the manometer between intake pipe and the gas bomb, the valve is installed at the top of gas bomb, one side that the gas bomb is close to the box is connected with the outlet duct, the one end that the gas bomb was kept away from to the outlet duct is connected inside cultivateing the storehouse.
Compared with the prior art, the utility model, following beneficial effect has:
(1) the utility model discloses top cap and chucking device have been set up, the research that carries out the cultivation under the oxygen deficiency environment carries out the in-process, not only need be through the observation of naked eye, the in-process that goes on in the experiment can have different circumstances to take place, probably need carry out relevant operation to the culture dish, thereby can in time open the incubator through the top cap and accomplish relevant operation to the culture dish, chucking device can guarantee the inseparable cooperation between top cap and the box, the seal of culture box has been guaranteed again after closing the top cap, the efficiency of cultivation work has been improved.
(2) The utility model discloses set up the constant temperature storehouse, thereby the constant temperature storehouse has guaranteed its survival condition for the cell provides corresponding temperature, thereby the motor drives the heating tube and rotates inside the constant temperature storehouse and guaranteed that the heat supply is even and then can provide suitable temperature environment to the cultivation storehouse of top of the body, has improved culture apparatus's operating mass with rated load.
(3) The utility model discloses set up the gas bomb, can also supply the oxygen deficiency type gas of different concentrations to the oxygen deficiency type gas of the required difference of different cells of transport in the bottle through the intake pipe of gas bomb, inside rethread outlet duct transportation to cultivateing the storehouse, facilitated for the cultivation work of oxygen deficiency cell.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is an enlarged view of the utility model at A;
FIG. 3 is an enlarged view of the utility model at B;
FIG. 4 is a top view of the cultivation container of the present invention;
in the figure: 1. a gas cylinder; 2. a box body; 3. a base; 4. a top cover; 5. a culture bin; 6. a constant temperature bin; 7. an air inlet pipe; 8. a pressure gauge; 9. a valve; 10. an air outlet pipe; 11. a motor; 12. a rotating shaft; 13. a bearing; 14. a heater; 15. a heat supply pipe; 16. a base plate; 17. a culture dish; 18. a support leg; 19. a hinge; 20. a rubber pad; 21. a clamping device; 210. a boss; 211. a snap ring; 212. a handle.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides the following technical solutions: the utility model provides a simple and easy cell oxygen deficiency culture apparatus, including box 2, the inside top position of box 2 is provided with cultivates storehouse 5, the inside below position of box 2 is provided with constant temperature storehouse 6, constant temperature storehouse 6 sets up in the below position of cultivateing storehouse 5, the top of box 2 is provided with top cap 4, top cap 4 is located the top of cultivateing storehouse 5, the bottom fixedly connected with base 3 of box 2, the bottom fixedly connected with landing leg 18 of base 3, landing leg 18 is provided with four altogether, four landing legs 18 distribute respectively in the geometry four corners of base 3.
Further, outside one side of top cap 4 rotates and is connected with hinge 19, hinge 19's one end is connected in top cap 4's outer wall, hinge 19's the other end is connected in box 2's outer wall, box 2 passes through hinge 19 with top cap 4 and rotates and then can make top cap 4 and box 2 close to be connected or the separation is opened through the hinge, top cap 4 and box 2 are connected the position and are provided with rubber pad 20, rubber pad 20 fixed connection is in top cap 4 bottom, rubber pad 20 can play the effect of filling the gap when top cap 4 and box 2 close, the leakproofness of whole incubator has been guaranteed.
Furthermore, the other side of top cap 4 relative to hinge 19 is provided with chucking device 21, chucking device 21 includes boss 210, boss 210 fixed connection is in the outer wall of top cap 4, boss 210 chucking is connected with snap ring 211, when needs open top cap 4, snap ring 211 just does not chucking in boss 210, handle 212 is installed to the position that the outer wall of box 2 is close to boss 210, the outside rotation of handle 212 is connected with snap ring 211, the one end chucking that handle 212 was kept away from to snap ring 211 is connected in the boss 210 outside, when needs open top cap 4, the researcher is through upwards moving handle 212 and then make snap ring 211 break away from in boss 210 thereby open the top cap, rubber pad 20 is also installed to top cap 4 bottom on one side of chucking device 21 and is played the effect of reinforcing leakproofness.
Specifically, the internally mounted of base 3 has motor 11, motor 11's model is Y80M1-2, motor 11's output is connected with pivot 12, the one end that motor 11 was kept away from to pivot 12 is connected with heater 14, be provided with bearing 13 between pivot 12 and the heater 14, heater 14's upper end fixedly connected with heating pipe 15, heating pipe 15 sets up inside constant temperature storehouse 6, thereby motor 11 drives heating pipe 15 and rotates and make heating pipe 15 can be even the work of dispelling the heat.
It is worth explaining, the inside bottom plate 16 that is provided with of culture bin 5, the both sides fixed connection of bottom plate 16 is in culture bin 5 inner wall, and the top of bottom plate 16 is provided with culture dish 17, and culture dish 17 is provided with a plurality of, is made by the polyethylene material, and the inside cell that has all cultivateed of every culture dish 17, culture dish 17 inside has set up the required liquid environment of cell survival and the required relevant material of cell survival.
Further, outside one side of box 2 is provided with gas bomb 1, one side that box 2 was kept away from to gas bomb 1 is connected with intake pipe 7, can be to the gas of the required oxygen deficiency type of 1 inside supplementary different cells of gas bomb and different concentration through intake pipe 7, install manometer 8 between intake pipe 7 and the gas bomb 1, manometer 8 can monitor the inside atmospheric pressure of gas bomb 1, valve 9 is installed at the top of gas bomb 1, one side that gas bomb 1 is close to box 2 is connected with outlet duct 10, the one end that gas bomb 1 was kept away from to outlet duct 10 is connected inside culture chamber 5, open valve 9 and can make the gas of 1 inside storage of gas bomb get into to the inside work that carries out of incubator.
The utility model discloses a theory of operation and use flow: when the utility model is used, firstly, the oxygen-deficient gas required for culturing the oxygen-deficient cells is input into the gas storage bottle 1 through the gas inlet pipe 7, the gas pressure in the gas storage bottle can be observed through the observation pressure gauge 8 while the oxygen-deficient gas is input, then the oxygen-deficient gas can be conveyed into the culture bin 5 of the incubator through the gas outlet pipe 10, a plurality of culture dishes 17 are arranged above the bottom plate 16 in the culture bin 5 so as to perform related culture work on the cells, the constant temperature bin 6 is arranged below the culture bin 5, the motor 11 drives the rotating shaft 12 to rotate so as to enable the heat supply pipe 15 to rotate in the constant temperature bin 6, and the proper temperature is provided for the culture bin 5 so as to provide proper conditions for the survival of the cells;
when needing to open the incubator and carry out relevant operation to culture dish 17, need open the separation with top cap 4 and box 2, thereby researcher makes snap ring 211 break away from boss 210 and no longer carries out the chucking through upwards lifting handle 212 and connects, and then opens top cap 4, thereby all be provided with rubber pad 20 in the junction of top cap 4 and box 2 and guaranteed to close the leakproofness of whole box 2 after top cap 4.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a simple and easy cell oxygen deficiency culture apparatus, includes box (2), its characterized in that: the inside top position of box (2) is provided with cultivates storehouse (5), the inside below position of box (2) is provided with constant temperature storehouse (6), the top of box (2) is provided with top cap (4), top cap (4) are located the top of cultivateing storehouse (5), the bottom fixedly connected with base (3) of box (2), the bottom fixedly connected with landing leg (18) of base (3), landing leg (18) are provided with four altogether.
2. The simple device for culturing cells in the absence of oxygen as claimed in claim 1, wherein: the outside one side of top cap (4) is rotated and is connected with hinge (19), the one end of hinge (19) is connected in the outer wall of top cap (4), the other end of hinge (19) is connected in the outer wall of box (2), box (2) and top cap (4) are connected through hinge (19) rotation, top cap (4) and box (2) position of being connected are provided with rubber pad (20), rubber pad (20) fixed connection is in top cap (4) bottom.
3. The simple device for culturing cells in the absence of oxygen as claimed in claim 1, wherein: top cap (4) are provided with chucking device (21) for the opposite side of hinge (19), chucking device (21) include boss (210), boss (210) fixed connection is in the outer wall of top cap (4), boss (210) chucking is connected with snap ring (211), handle (212) are installed to the position that box (2) outer wall is close to boss (210), the outside of handle (212) is rotated and is connected with snap ring (211), the one end chucking that handle (212) were kept away from in snap ring (211) is connected in boss (210) outside.
4. The simple device for culturing cells in the absence of oxygen as claimed in claim 1, wherein: the internally mounted of base (3) has motor (11), the output of motor (11) is connected with pivot (12), the one end that motor (11) were kept away from in pivot (12) is connected with heater (14), be provided with bearing (13) between pivot (12) and heater (14), the upper end fixedly connected with heating pipe (15) of heater (14), heating pipe (15) set up inside constant temperature storehouse (6).
5. The simple device for culturing cells in the absence of oxygen as claimed in claim 1, wherein: cultivate storehouse (5) inside and be provided with bottom plate (16), the both sides fixed connection of bottom plate (16) is in cultivating storehouse (5) inner wall, the top of bottom plate (16) is provided with culture dish (17).
6. The simple device for culturing cells in the absence of oxygen as claimed in claim 1, wherein: the utility model discloses a cultivation box, including box (2), valve (9), the outside one side of box (2) is provided with gas bomb (1), one side that box (2) were kept away from in gas bomb (1) is connected with intake pipe (7), install manometer (8) between intake pipe (7) and gas bomb (1), valve (9) are installed at the top of gas bomb (1), one side that gas bomb (1) is close to box (2) is connected with outlet duct (10), the one end that gas bomb (1) was kept away from in outlet duct (10) is connected inside culture storehouse (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022081749.7U CN213506979U (en) | 2020-09-22 | 2020-09-22 | Simple and easy cell oxygen deficiency culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022081749.7U CN213506979U (en) | 2020-09-22 | 2020-09-22 | Simple and easy cell oxygen deficiency culture apparatus |
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| Publication Number | Publication Date |
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| CN213506979U true CN213506979U (en) | 2021-06-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022081749.7U Active CN213506979U (en) | 2020-09-22 | 2020-09-22 | Simple and easy cell oxygen deficiency culture apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113604332A (en) * | 2021-07-20 | 2021-11-05 | 西南医科大学附属中医医院 | Disposable oxygen-deficient environment cell culture bag |
-
2020
- 2020-09-22 CN CN202022081749.7U patent/CN213506979U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113604332A (en) * | 2021-07-20 | 2021-11-05 | 西南医科大学附属中医医院 | Disposable oxygen-deficient environment cell culture bag |
| CN113604332B (en) * | 2021-07-20 | 2023-09-19 | 西南医科大学附属中医医院 | Disposable anaerobic environment cell culture bag |
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