TWI567190B - Cell incubator - Google Patents

Description

Cell culture incubator

This creation is about a cell culture device, and in particular, it relates to a cell culture incubator which is light in appearance and convenient for use.

In view of the booming biotechnology, cell culture has become a hot spot in recent years. In order to achieve sterile culture of cells, the general method can be carried out in a sterile cell culture chamber. However, the environmental requirements for cell culture are quite strict. Once any link does not meet the standard, the efficiency of cultivation will be greatly reduced, and even cultivated. Invalid.

For this reason, because the equipment of the large cell culture room is excellent and the relative overall cost is also high, there are few sterile cell culture rooms in a single area, and generally only regional hospitals, biochemistry colleges or large inspection institutes. This equipment may be available. Therefore, if there is a need for cell culture, it is not only necessary to go to a large hospital, but also to inconvenient traffic. It is also necessary to schedule the time in advance, which will cause time and pain in the next day, which is quite inconvenient. .

The cell culture incubator of the present invention has a small overall volume and a light appearance, which is not only convenient for movement, but also becomes a sterile closed environment after the cycle installation of each cell culture is completed, and then can be moved to a lower specification environment to realize the present. Create automatic, timed and quantitative automatic culture of cells without the need for large sterile rooms and manual operations. Therefore, this creation can save high cost of plant facilities and equipment and avoid the risk of pollution caused by artificial cultivation, and is more convenient to expand. Advantage.

Therefore, a cell culture incubator proposed by the present invention comprises: a box body having an inner space, and an airtight state refrigerating chamber located above the inner space, and an airtight state culture chamber. The chamber is configured to store a culture medium, wherein the culture chamber has at least one culture bag; a first cover and a second cover, the first cover is airtightly disposed on the culture chamber, and the second cover is An airtight cover is disposed on the refrigerating compartment; an electronic control unit has a microprocessor, a power module, a digital/analog conversion module connected between the microprocessor and the power module, and a a heating module for controlling the temperature of the culture chamber, a cooling module for supplying a cold source to the refrigerating chamber, a peristaltic pump module, a carbon dioxide supply for supplying carbon dioxide to the culture chamber and detecting a carbon dioxide concentration of the culture chamber And the detecting module, and a setting display module exposed on the surface of the first cover, the peristaltic pump module is aseptically connected between the culture solution and the culture bag by using one or more infusion tubes, by the Power supply from the power module, and through the device The condition of the preset cell culture of the display module is controlled by the microprocessor, so that the peristaltic pump module extracts the culture liquid into the culture bag.

In summary, the biggest advantage of this creation is that it adopts a closed aseptic environment culture, so the risk of contamination during artificial culture is avoided, and the success rate of cell culture is greatly improved, and the present invention has the following effects and advantages:

1. Since the setting display module is disposed on the surface of the first cover, the desired setting value can be directly set on the liquid crystal panel (LCD) during operation, which can be conveniently used.

2. The first cover and the transparent glass independently pivot the installation of the box body, and directly open the first cover, the culture chamber can be seen through the transparent glass to observe the culture condition, and the transparent glass is pressed On the gasket, to maintain the airtightness of the culture chamber, the culture environment is more stable.

Third, the creation uses a resistive heating piece for heating, and through the microprocessor with the sensing control of each of the sensors, not only can accurately control the temperature of the culture chamber to maintain the preset temperature, and the temperature is not too high. The problem of control.

Fourth, through the peristaltic pump module, the peristaltic supply medium is matched with the diameter of the infusion tube, and the injected dose will be more accurate.

5. Before the culture liquid is transported to the culture bag through the infusion tube, the protective tube is covered with the silicone material when the infusion tube passes through the cold room to the peristaltic pump module chamber and the self-creeping pump module chamber passes through the culture chamber. Therefore, it is possible to avoid different temperature disturbances in each tank and to have the function of isolating air.

10‧‧‧Box body

101‧‧‧ first through hole

102‧‧‧Second through hole

103‧‧‧ protective cover

11‧‧‧Internal space

12‧‧‧Refrigerator

13‧‧‧Cultivation room

131‧‧‧ platform

132‧‧‧Water tray

14‧‧‧Peristal pump module room

15‧‧‧Washers

16‧‧‧Washers

17‧‧‧ Washer

18‧‧‧ grip

19‧‧‧Internal space

20‧‧‧First lid

30‧‧‧Second lid

40‧‧‧Electronic control unit

41‧‧‧Microprocessor

42‧‧‧Power Module

43‧‧‧Digital/Analog Conversion Module

44‧‧‧heating module

441‧‧‧ heating film

442‧‧‧copper

45‧‧‧ Refrigeration module

46‧‧‧ creeping pump module

461‧‧‧stepper motor

47‧‧‧CO2 detection module

471‧‧‧Air intake pipe

472‧‧‧Exhaust pipe

473‧‧‧First vent

474‧‧‧second vent

475‧‧‧ Carbon dioxide detector

476‧‧‧Air outlet

477‧‧‧pipe

48‧‧‧Set display module

49‧‧‧Temperature Sensing Module

491‧‧‧ sensor

50‧‧‧ pivotal parts

51‧‧‧ pivotal parts

60‧‧‧Lock group

70‧‧‧clear glass

80‧‧‧Insulation materials

90‧‧‧Vacuum insulation board

100‧‧‧ culture fluid container

110‧‧‧Cultivation bags

120‧‧‧Infusion tube

130‧‧‧ sterile filter ring

140‧‧‧ culture solution

Figure 1 is an external perspective view of a preferred embodiment of a cell culture incubator.

Fig. 2 is a perspective view showing another appearance of the cell culture incubator.

Figure 3 is a perspective view showing the appearance of the first and second tank lids removed from the cell culture incubator.

Figure 4 is a top plan view of the cell culture incubator showing the insulating material filled between the refrigerating chamber, the peristaltic pump module chamber, and the culture chamber.

Figure 5 is a cross-sectional view of the cell culture incubator.

Figure 6 is another cross-sectional view of the cell culture incubator.

Figure 7 is a perspective view showing the appearance of the culture chamber and the carbon dioxide detecting module.

Figure 8 is a bottom view of the cell culture incubator.

Figure 9 is a circuit block diagram of the electronic control unit.

Fig. 10 is a schematic view showing the aseptic connection of the culture solution container and the cell culture container.

As shown in Fig. 10, the present invention utilizes a culture solution container 100 and a culture bag 110 to be previously sleeved together with an infusion tube 120 on an aseptic processing table and a sterile filter ring 130, thus completing a sterile closed system. . From the above operation, this creation integrates a small mobile closed system integrating refrigeration and heat preservation and liquid transfer, which greatly improves the cell culture. power. The culture solution container 100 is filled with a culture solution 140.

Referring to FIG. 1 , in a preferred embodiment of the present invention, the cell culture incubator includes a box body 10 , a first box cover 20 , and a second box cover 30 , and an electronic control unit 40 . .

Referring to Figures 2, 5 and 6, the box body 10 has an internal space 11 and a refrigerating chamber 12 located above the internal space 11 and a culture chamber 13 in an airtight state. The refrigerating chamber 12 The culture solution container 100 is stored, and the culture chamber 13 has at least one culture bag 110 built therein.

The first cover 20 is airtightly disposed on the culture chamber 13, and the second cover 30 is airtightly disposed on the refrigerating chamber 12.

Referring to FIGS. 6-9, the electronic control unit 40 has a microprocessor 41, a power module 42, and a digital/analog conversion connected between the microprocessor 41 and the power module 42. a module 43, a heating module 44 for controlling the temperature of the culture chamber 13, a cooling module 45 for providing a cold source to the refrigerating chamber 12, a peristaltic pump module 46 having a stepping motor 461, and a A carbon dioxide detecting and supplying module 47 for supplying carbon dioxide to the culture chamber 13 and a setting display module 48 exposed on the surface of the first lid 20 are provided. The microprocessor 41, the power module 42 and the digital/analog conversion mode 43 are all disposed in the internal space 11 of the box body 10. The peristaltic pump module 46 is connected between the culture fluid container 100 and the culture bag 110 by a plurality of infusion tubes 120, and is powered by the power module 42 and preset by the setting display module 48. The condition of the cell culture is controlled by the microprocessor 41 so that the peristaltic pump module 46 draws the culture solution 140 into the culture bag 110 via the stepping motor 461.

Further, as shown in FIG. 6, the cooling module 45 is disposed on an outer sidewall of the box body 10, and has two cooling fins 451, two cooling blocks 452 corresponding to the refrigerating chamber 12, and two sets of heat sinks. 453, so that the cooling module 45 transmits the cold source of the cooling wafer 451 to the refrigerating chamber 12 via the cooling block 452, and the refrigeration chamber 12 is controlled by the control of the microprocessor 41. It is best to maintain the temperature at 4~6 °C.

The box body 10 further has a peristaltic pump module chamber 14 on the side of the refrigerating chamber 12 and the cultivating chamber 13, and the peristaltic pump module 46 is placed in the peristaltic pump module chamber 14, and the The second cover 30 is simultaneously disposed on the refrigerating chamber 12 and the peristaltic pump module chamber 14, and the refrigerating chamber 12 and the top periphery of the peristaltic pump module chamber 14 are respectively provided with a washer 15, 16 so that When the second cover 30 is closed to the refrigerating chamber 12 and the peristaltic pump module chamber 14, the gaskets 15 and 16 are brought into close contact with each other to achieve airtightness. In addition, as shown in FIG. 5, a transparent glass 70 is disposed between the culture chamber 13 and the first cover 20, and a gasket 17 is disposed on the top periphery of the culture chamber 13 to cover the transparent glass 70. At 1300, the gasket 17 is brought into close contact with each other to achieve airtightness, thereby facilitating cell culture.

As shown in FIG. 4, the cell culture incubator further includes a heat insulating material 80 filled in the refrigerator compartment 12, the creeping pump module chamber 14 and the culture chamber 13 In the interior space 19, the heat insulating material 80 is PE, which serves as a heat insulating effect of the three tank chambers. In addition, a vacuum insulation panel 90 may be further disposed between the refrigerating compartment 12 and the culture chamber 13, and the vacuum insulation panel 90 is attached to the outer sidewall of the refrigerating compartment 12, and the vacuum insulation panel 90 (Vacuum Insulation Panel) is A sheet of vacuum-protected surface layer filled with a core material, vacuum-sealed, and then heat-sealed. Since it effectively avoids air convection heat transfer, it is possible to prevent the low temperature of the refrigerating compartment 12 from affecting the thermal temperature of the culture chamber 13.

As shown in the first, second, and fifth figures, the cell culture incubator further includes a plurality of pivoting members 50, 51, and a plurality of locking groups 60 mounted on the first and second tank covers 20, 30 and the box body 10. The first cover 20 and the second cover 30 are pivotally mounted on the box body 10 by the pivot members 50, so that the first cover 20 and the second cover 30 can open and close the case. . The transparent glass 70 is separately pivoted on the box body 10 by the pivoting member 51, so that the transparent glass 70 and the first box cover 20 can be individually lifted up. Moreover, when the first and second tank covers 20, 30 are in the closed position, the first and second tank covers 20, 30 are locked in the fixed position by the respective lock sets 60.

As shown in FIG. 3, the box body 10 further has a first through hole 101, a second through hole 102, and a plurality of protective sleeves 103. The first through hole 101 communicates with the refrigerating chamber 12 and the creeping pump module. Between the chambers 14, the second through hole 102 is connected between the peristaltic pump module chamber 14 and the culture chamber 13, and the infusion tube 120 extending outward from the refrigerating chamber 12 passes through the first through hole 101. And connected to the peristaltic pump module 46, then through the second through hole 102, and connected to the culture chamber 13.

Before the cell culture, the culture solution container 100, the culture bag 110, the protective cover 103, and the infusion tube 120 are aseptically assembled in an aseptic environment, and then the culture solution container 100 is placed in the refrigerator compartment 12 to maintain the temperature at 4 °C. ~6 ° C for cold preservation, and then the infusion tube 120 and the peristaltic pump module 46 as a hanging tube, so that the peristaltic pump module chamber 14, and then the culture bag 110 is placed in the cultivation chamber 13, and each The protective sleeves 103 are respectively fixed on the first and second through holes 101 and 102, so that the pipe sections of the infusion pipes 120 passing through the first and second through holes 101 and 102 can pass through the box through the protective cover 103. Each of the through holes of the main body 10 is preferably the same as the silicone material in the embodiment. Avoid temperature and gas interference. Finally, the first cover 20 and the second cover 30 are respectively airtightly covered by the refrigerating chamber 12, the peristaltic pump module chamber 14 and the culture chamber 13, and directly touched by the setting display module 48. By controlling the time of cell culture, the amount of pump delivery, temperature, humidity, etc., subsequent cell culture operations can be performed.

Further, as shown in FIGS. 3 and 5, the culture chamber 13 is internally provided with a hollow platform 131 for arranging the culture bag 110, and a water tray 132 located below the platform 131. The platform 131 is arranged in a hollow manner for the purpose of communicating with the water tray 132. During the cell culture process, the water tray 132 contains the sterile water solution and controls the culture chamber 13 to have a certain saturated humidity.

As shown in FIGS. 5 and 7, the carbon dioxide detecting module 47 includes an air inlet pipe 471 and an exhaust pipe 472 disposed on the outer wall surface of the box body 10, and a wall surface disposed on the culture chamber 13. A first air hole 473 and a second air hole 474 are disposed, and a carbon dioxide detector 475 and an air outlet mechanism 476 disposed in the culture chamber 13. The intake pipe 471 is connected to the first air hole 473 by a pipe 477 to introduce carbon dioxide from the outside, and the exhaust pipe 472 is connected to the second air hole 474 by another pipe 477 to Do pressure relief. The air outlet mechanism 476 performs internal air circulation on the culture chamber 13. The carbon dioxide detector 475 is electrically connected to the microprocessor 41 to detect the concentration of carbon dioxide in the culture chamber 13 and control the carbon dioxide concentration at a preset. Set a percentage. In the present case, the air outlet mechanism 476 is any one of a fan and a blower.

The heating module 44 is supplied with electric power by the power module 42 and is spaced apart from an outer wall surface of the culture chamber 13 and has a plurality of resistive heating fins 441 and a plurality of copper attached to each of the heating fins 441. The piece 442 is composed of.

The electronic control unit 40 further includes a temperature sensing module 49 electrically connected to the microprocessor 41, and includes a plurality of sensors 491. Each of the sensors 491 is disposed in the middle and the outside of the culture chamber 13 for use. The indoor and outdoor temperatures of the culture chamber 13 are sensed. In this case, the indoor and outdoor temperatures of the culture chamber 13 are sensed by each of the sensors 491 to control the temperature of the culture chamber 13 to a preset temperature. If the temperature does not reach the desired temperature, the microprocessor 41 is passed through the microprocessor 41. The automatic control will activate the heating module 44 to perform a warming operation to maintain the desired ambient temperature in the cell culture.

As shown in Figures 3 and 9, the cell culture chamber is controlled by the electronic control unit 40, and the power supply module 42 supplies the required power, as long as the setting is performed via the setting display module 48. When the cell culture operation is performed, the carbon dioxide detecting device 47 supplies a valve for injecting and discharging carbon dioxide through the calculation processing of the microprocessor 41 to control the concentration of carbon dioxide in the culture chamber 13 in advance. The set percentage is further heated by the heating unit 44 to control the temperature in the culture chamber 13 to a preset temperature, and the cold source is supplied by the refrigeration module 45 to maintain the temperature of the refrigerating chamber 12 at a preset refrigeration temperature. The temperature is sensed by the temperature sensing module 49. When the temperature is changed by the outside, the microprocessor 41 adjusts the correction at any time to ensure that the optimal culture environment and conditions are in the portion of the supplemental culture solution 140. Is the The microprocessor 41 outputs a signal, and the signal is converted by the digital/analog conversion module 43 to change the rotation speed of the stepping motor 461 and matched with the diameter of the infusion tube 210, so that the culture liquid 140 can be driven into the culture by the peristaltic method. The bag 110 is completed, and when the set time is up, the batch metering cultivation operation is completed.

In addition, as shown in the first and second figures, the box body 10 further includes a plurality of grips 18, and the grips 18 are respectively disposed on both sides of the box body 10 and the first box cover 20 and the second box cover 30. The setting of the grip 18 not only facilitates the convenience of carrying the carrying, but also makes it easier to operate when the lid is opened.

According to the above, the cell culture incubator of the present invention has a small overall size and a light appearance, which is convenient to carry, so as to facilitate the movement of cells to various places for cell cultivation, and is no longer limited by the inconvenience of relying on the use of a large cell culture room. Or you must pre-schedule time and so on. In addition, after the cycle installation of each cell culture is completed, it becomes a sterile closed environment, and then can be moved to a lower specification environment to realize the automatic timing and automatic cultivation of cells of the creation without the need for a large sterile room and labor. Therefore, this creation can save the cost of high plant facilities and equipment and avoid the risk of pollution caused by artificial cultivation, and has the advantage of expanding convenience. Secondly, compared with the existing large cell culture room, the cost of the cell culture incubator is relatively cheap and easy to use, suitable for purchase by various units, and the cost is relatively low.

In summary, the cell incubator of the present invention has the following effects and advantages:

1. Since the setting display module 48 is disposed on the surface of the first cover 20, the desired setting value can be directly set on the liquid crystal panel (LCD) during operation, which can be conveniently used.

2. The first cover 20 and the transparent glass 70 independently erect the installation of the box body 10, and directly open the first cover 20, so that the inside of the culture chamber 13 can be seen through the transparent glass 70 to observe the culture condition. Further, the transparent glass 70 is pressed against the gasket 17 to maintain the airtightness of the culture chamber 13, and the culture environment is more stable.

3. The present invention uses a resistive heating pad 441 for heating, and the microprocessor 41 cooperates with the sensing control of each of the sensors 491 to accurately control the temperature of the culture chamber 13 It is maintained at a preset temperature and there is no problem that the temperature is too high to control.

4. The culture fluid 140 is supplied by the stepping motor 461 in a peristaltic manner, and the dose to be injected is more precise.

5. Before the culture fluid 140 is delivered to the culture bag 110 via the infusion tube 120, since the infusion tube 120 passes through the refrigerating chamber 12 to the peristaltic pump module chamber 14 and the peristaltic pump module chamber 14 passes through the culture chamber 13, The protective cover 103 is coated with a silicone material, so that different temperature disturbances of each chamber can be avoided and the function of isolating air can be avoided.

The above is only a preferred embodiment of the present invention. When it is not possible to limit the scope of the creation of the present invention, the simple equivalent changes and modifications made by the applicant in accordance with the scope of the patent application and the new description of the creation should remain This creative patent covers the scope.

10‧‧‧Box body

18‧‧‧ grip

20‧‧‧First lid

30‧‧‧Second lid

40‧‧‧Electronic control unit

471‧‧‧Air intake pipe

472‧‧‧Exhaust pipe

48‧‧‧Set display module

50‧‧‧ pivotal parts

51‧‧‧ pivotal parts

Claims (17)

A cell culture incubator comprising: a box body having an inner space, and a refrigerating chamber and an cultivating chamber located in the inner space and in an airtight state, the refrigerating chamber storing a culture liquid, the cultivating chamber Having at least one culture bag; a first cover and a second cover, the first cover is airtightly disposed on the culture chamber, and the second cover is airtightly disposed on the cold storage; The electronic control unit has a microprocessor, a power module, a digital/analog conversion module connected between the microprocessor and the power module, and a heating module capable of controlling the temperature of the culture chamber. a cooling module providing a cold source to the refrigerating chamber, a peristaltic pump module, a carbon dioxide supply and detection module for supplying carbon dioxide to the culture chamber, and a setting display exposed on the surface of the first cover a module, the peristaltic pump module uses one or more infusion tubes to be aseptically connected between the culture solution and the culture bag, and is powered by the power module, and preset cell culture is performed through the set display module. Condition, and then through the microprocessor operation control, So that the peristaltic pump module draws the culture solution into the culture bag. The cell culture incubator of claim 1, wherein the box body further has a peristaltic pump module chamber located on the refrigerating chamber and the side of the culture chamber, and the peristaltic pump module is placed in the peristaltic In the pump module chamber, the second cover is simultaneously disposed on the refrigerating chamber and the peristaltic pump module chamber, and a gasket is disposed on each of the refrigerating chamber and the top periphery of the peristaltic pump module chamber to When the second cover is closed in the refrigerating chamber and the peristaltic pump module chamber, the gasket is brought into close contact with each of the gaskets to achieve airtightness. The cell culture incubator of claim 2, wherein the tank body has a first through hole and a second through hole, the first through hole being connected to the refrigerating chamber and the peristaltic pump module chamber The second through hole is connected between the peristaltic pump module chamber and the culture chamber, and the infusion tube extending outward from the refrigerating chamber passes through the first through hole, and the peristaltic pump module Link, then wear the second pass A well and connected to the culture chamber. The cell culture incubator of claim 3, further comprising a plurality of protective sleeves respectively disposed on the outer sides of the infusion tubes, wherein the protective sleeves are respectively fixed on the first and second through holes, The pipe sections passing through the first and second through holes of each of the infusion pipes can pass through the through holes of the box body through the protective sleeve, and each of the protective sleeves is made of silicone rubber. The cell culture incubator according to claim 1, wherein the culture chamber is provided with a hollow platform for arranging the culture bag, and a water tray located below the platform. The cell culture incubator of claim 1, wherein a clear glass that is airtightly disposed on the culture chamber is disposed between the culture chamber and the first cover, and a gasket is disposed on a periphery of the top of the culture chamber. When the transparent glass is covered in the culture chamber, it is brought into close contact with the gasket to achieve airtightness. The cell culture incubator of claim 6, wherein the first cover is pivotally mounted on the box body by a plurality of pivoting members, and the transparent glass is separately pivoted by a pivoting member. The box body is such that the transparent glass and the first box cover are individually lifted. The cell culture incubator of claim 1, wherein the microprocessor, the power module, and the digital/analog conversion module are disposed in an inner space of the box body. The cell culture incubator of claim 1, wherein the refrigeration module is disposed on an outer side wall of the box body, and has at least one cold wafer, at least one cold block corresponding to the cold chamber, and At least one set of fins for causing the refrigeration module to transport the cold source of the cooled wafer to the refrigerating chamber via the cold guide block. The cell culture incubator according to claim 1, wherein the heating module is supplied with electric power by a power module, is installed at an outer wall surface of the culture chamber at intervals, and is composed of a plurality of resistive heating sheets and a plurality of The copper sheet is attached to each of the heating sheets. The cell culture incubator of claim 1, wherein the carbon dioxide supply and detection module comprises an air inlet pipe and an exhaust pipe disposed on an outer wall surface of the box body, and the a first air hole and a second air hole on a wall of the curing room, and a carbon dioxide detector and an air outlet mechanism disposed in the culture chamber, the air inlet tube being connected to the first air hole by a pipeline Introducing carbon dioxide from an external guide, the exhaust pipe is connected to the second air hole for decompression, and the air outlet mechanism performs internal air circulation to the culture chamber, the carbon dioxide detector Electrically coupled to the microprocessor to detect the concentration of carbon dioxide in the chamber. The cell culture incubator of claim 1, further comprising a temperature sensing module electrically connected to the microprocessor, comprising a plurality of sensors, each of the sensors being placed in the culture The middle and outer sides of the chamber are used to sense the indoor and outdoor temperatures of the culture chamber. The cell culture incubator of claim 1, further comprising a plurality of pivoting members, a plurality of latching groups mounted on the first and second tank covers and the box body, the first box The cover and the second cover are pivotally disposed on the box body by the pivoting members, and when the first and second cover are in the closed position, the first and second boxes are used by each of the lock sets The cover is locked in a fixed position. The cell culture incubator of claim 1, further comprising a plurality of grips, each of the grips being respectively disposed on both sides of the box body and the first box cover and the second box cover. The cell culture incubator of claim 1, wherein the peristaltic pump module has a stepping motor. The cell culture incubator of claim 1, further comprising a heat insulating material filled in the refrigerator compartment, the creeping pump module chamber and the culture chamber Inside the interior space. The cell culture incubator of claim 1, further comprising a vacuum insulation panel attached to the outer side wall of the refrigerator compartment.