CN113736651A - Automatic cell culture device - Google Patents
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- CN113736651A CN113736651A CN202010479892.3A CN202010479892A CN113736651A CN 113736651 A CN113736651 A CN 113736651A CN 202010479892 A CN202010479892 A CN 202010479892A CN 113736651 A CN113736651 A CN 113736651A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 93
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 96
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 48
- 239000001569 carbon dioxide Substances 0.000 claims description 48
- 238000001802 infusion Methods 0.000 claims description 42
- 238000005192 partition Methods 0.000 claims description 17
- 230000005540 biological transmission Effects 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000013459 approach Methods 0.000 claims description 6
- 238000003860 storage Methods 0.000 description 10
- 239000011229 interlayer Substances 0.000 description 6
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 6
- 238000009413 insulation Methods 0.000 description 5
- 238000005057 refrigeration Methods 0.000 description 5
- 239000010410 layer Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 3
- 238000001816 cooling Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000002937 thermal insulation foam Substances 0.000 description 2
- -1 acryl Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention provides an automatic cell culture device, which comprises a body, wherein the body is provided with a culture chamber and a transfusion hole communicated with the culture chamber and an external space, a user can put a cell culture bag in the culture chamber, and a transfusion pipe for supplying culture solution to the cell culture bag is arranged to penetrate through the transfusion hole and is connected with a transfusion source outside the body.
Description
Technical Field
The present invention relates to a cell culture apparatus.
Background
In recent years, due to the progress of medical technology, a large amount of cells have been required to be cultured in the fields of drug development and production, gene therapy, immunotherapy, cell therapy, and the like.
For example, as described in taiwan patent No. TWI670370, a conventional cell culture apparatus is mainly configured such that a large-sized incubator having a heat insulating wall is used, a culture medium and culture containers are connected to each other and then placed in a cooling tank and a culture tank which are separated from each other in the left and right directions, the cooling tank is maintained at 4 ℃ to store the culture medium, the culture tank is maintained at 37 ℃ to perform cell culture, a plurality of culture containers are stored in a multi-layered shelf, and a measurement unit such as a camera is guided by a support column to move and observe the cell culture state of each culture container, thereby achieving automated and mass production.
Although the cell culture apparatus of taiwan patent TWI670370 can provide mass production, the cell culture apparatus has a large scale, and the cooling-maintaining tanks and the culture tanks separated from each other at different temperatures require further refrigeration equipment, which makes the whole equipment of the cell culture apparatus larger and thus is not available in large laboratories or large medical institutions. In addition, although a plurality of culture containers can be placed in the culture tank, the user needs to pay attention to whether the culture containers interfere with each other and are contaminated, whether the internal environment of the culture tank is constant when the culture containers are taken out or placed in the culture tank, and whether the shelf hinders sterilization and maintenance of a sterile environment, which is inconvenient in practical operation and use.
On the other hand, the cell culture chamber described in taiwan patent No. TWI567190 is a cell culture chamber having a heat insulating wall, in which a refrigerating chamber, a culture chamber and a pump chamber are partitioned, a culture solution, a culture bag and a peristaltic pump module are respectively placed, and main equipment required for cell culture is integrated into a single box-shaped apparatus, thereby having the characteristics of miniaturization, rapid configuration and convenient movement. However, the cell culture box described in taiwan patent TWI567190 also has a refrigerating chamber and a culture chamber, and needs to maintain different temperatures in the box, even though a vacuum insulation panel is provided, it is not affected by natural flow of heat and consumes energy, and secondly, in order to maintain the low temperature of the refrigerating chamber, the cell culture box still needs to be provided with a refrigerating device, so that the volume of the cell culture box is not easy to further reduce, which is not beneficial to mass production of replication and stacking, and it is difficult to improve the space utilization rate.
Disclosure of Invention
In view of the above, one object of the present invention is to provide a cell culture apparatus, which is modularized and miniaturized for improving space utilization rate when performing cell culture.
The invention provides an automatic cell culture device, which is used for placing a cell culture bag and carrying out cell culture and comprises a body, wherein the body is provided with a culture chamber and a transfusion hole, and the transfusion hole is communicated with the culture chamber and an external space, so that a transfusion pipe can be connected between a transfusion source outside the body and the cell culture bag in the culture chamber through the transfusion hole when the cell culture bag is placed in the culture chamber.
The above-mentioned automatic cell culture apparatus can further comprise a base accommodated in the culture chamber, the base comprising a rocking base and a platform, the rocking base being capable of driving the platform to rock, the platform being for the cell culture bag to be placed thereon.
In the above-mentioned automatic cell culture apparatus, the carrier may include a tray body and a partition plate, the tray body is for holding water, the partition plate is for holding the cell culture bag, the partition plate is erected on the tray body to separate the water held in the tray body from the cell culture bag, and the partition plate has a plurality of openings for the water held in the tray body to evaporate and spread in the culture chamber.
The above-mentioned automatic cell culture apparatus can further comprise a temperature control module, the temperature control module comprises a heater and a temperature sensor, the temperature control module can sense the temperature of the culture chamber and selectively heat the culture chamber, the body has a first connector, the heater and the temperature sensor are electrically connected to the first connector, and the first connector is exposed from the surface of the body.
The above-mentioned automatic cell culture device can further comprise a carbon dioxide module, the carbon dioxide module comprises a carbon dioxide sensor, the carbon dioxide sensor is located in the culture chamber, the body has a second connector and a gas transmission hole, the carbon dioxide sensor is electrically connected to the second connector, the second connector is exposed from the surface of the body, and the gas transmission hole is communicated with the culture chamber and an external space to connect a carbon dioxide supply source outside the body.
In the above-mentioned automatic cell culture device, the body may further comprise a plug base, the plug base extends from the culture chamber to an external space, the infusion hole penetrates and is formed in the plug base, and the first connector and/or the second connector may be disposed on the plug base.
The above-mentioned automatic cell culture apparatus may further comprise a control module, the first connector is electrically connected to the control module, the control module detects the temperature of the culture chamber via the temperature sensor, and controls the heater to be turned on and off to make the temperature of the culture chamber approach 37 ℃. And secondly, the second connector is electrically connected with the control module, the control module detects the concentration of the carbon dioxide in the culture chamber through the carbon dioxide module and controls the carbon dioxide supply source to supply the carbon dioxide to the culture chamber through the gas transmission hole, so that the volume concentration of the carbon dioxide in the culture chamber approaches 5%.
Utilize above-mentioned device, automatic cell culture device can supply to put into the cell culture bag and carry out cell culture, and accept the external supply and give the culture solution, deposit the required thermal-insulated environment of culture solution and refrigeration plant need not to accompany by automatic cell culture device's culture room, can avoid depositing the required low temperature of culture solution and the high temperature interference influence of each other of culture room, maintain the invariant of cell culture environment, the structure of culture room can further be retrencied, it is miniaturized, and with each parts modularization, be favorable to the disinfection, remove, rapid configuration, reduce the cost of setting and improve space utilization.
Drawings
FIG. 1 is a schematic diagram of an automated cell culture apparatus according to the present invention.
FIG. 2 is a schematic view of a plug seat in the automated cell culture apparatus according to the present invention.
FIG. 3 is an exploded perspective view of a base of the automated cell culture apparatus of the present invention.
FIG. 4 is a schematic diagram of another embodiment of the automated cell culture apparatus of the present invention.
Description of reference numerals: a body 10; a bottom wall 11; a side wall 12; a top cover 13; a culture chamber 14; an observation plate 15; an infusion hole 16; a plug seat 17; a cut 171; a first connector 172; a second connector 173; a third connector 174; the gas transfer holes 175; a bleed valve 176; a base 20; a rocking base 21; a base 211; a drive rod 212; a pedestal 213; a tray body 221; a partition 222; a heater 31; a temperature sensor 32; a carbon dioxide sensor 41; a gas cylinder connector 51; the control valve 52; the gas supply pipe 53; an infusion tube 61; a holding tank 62; an infusion pump 63; a control module 70; a cell culture bag 90; a carbon dioxide high pressure cylinder 91.
Detailed Description
Referring to fig. 1, the present invention provides an automated cell culture apparatus for placing into a cell culture bag 90 for cell culture, the automated cell culture apparatus includes a main body 10, a base 20, a temperature control module, a carbon dioxide supply source, a transfusion source, and a control module 70.
The main body 10 is a box or box-shaped container, and includes a bottom wall 11, a side wall 12 and a top cover 13, wherein the side wall 12 extends upward from the periphery of the bottom wall 11, the top cover 13 is pivotally openably and closably disposed on the side wall 12, a culture chamber 14 is defined between the bottom wall 11, the side wall 12 and the top cover 13 for placing a cell culture bag 90 for cell culture, the bottom wall 11, the side wall 12 and the top cover 13 respectively have a heat insulation interlayer, specifically, the bottom wall 11, the side wall 12 and the top cover 13 are, for example, a multilayer structure having an inner layer stainless steel plate, an outer layer stainless steel plate, and a heat insulation foam interlayer or a vacuum interlayer between the inner layer and the outer layer, so that the culture chamber has heat insulation and heat preservation capabilities, and preferably, the bottom wall 11 and the heat insulation interlayer in the side wall 12 are integrally connected to each other, and can have better heat insulation and heat preservation capabilities. The main body 10 further has an observation plate 15, the observation plate 15 is made of a transparent plate material such as glass or acryl, and is pivotally openably disposed on the sidewall 12, and is located below the top cover 13, so that the user can keep covering the cultivation room 14 when opening the top cover 13, and the user can observe the inside of the cultivation room 14 and keep the environment inside the cultivation room 14 stable. The bottom wall 11, the side walls 12, the top cover 13 and the observation plate 15 are opened and closed upward, but if the top cover, the side walls and the bottom wall are made as a whole, and one of the side walls is opened and closed upward or in a door type, and the observation plate 15 is arranged inside the side wall, it is not necessary.
The main body further has an infusion hole 16, the infusion hole 16 is connected to the culture chamber 14 and the external space, an infusion tube 61 for delivering the culture solution can penetrate into the culture chamber 14 through the infusion hole 16 and is connected to a cell culture bag 90 in the culture chamber 14, and the culture solution is provided to the cell culture bag 90, specifically, referring to fig. 1 and 2, the main body further has a plug seat 17, a top edge of the sidewall 12 has a notch penetrating through the inner and outer surfaces, the plug seat 17 is disposed in the notch, so that the plug seat 17 extends from the culture chamber 14 to the external space and penetrates through the inner and outer surfaces of the sidewall 12, the infusion hole 16 is formed on the plug seat 17 and penetrates through the plug seat 17, and the infusion tube 61 can penetrate into the culture chamber 14 through the infusion hole 16, wherein preferably, the plug seat 17 can be partially or completely made of soft plastic or rubber and has elasticity, a plurality of slits 161 are circumferentially disposed around the infusion hole 16, so that the plug seat 17 can be deformed by being squeezed, so as to coat and adapt to infusion tubes 61 with different tube diameters; plug base 17 may also have a cutout 171, and cutout 171 extends from infusion port 16 to a top edge of plug base 17 to allow a user to push infusion tube 61 into infusion port 16 through cutout 171 when tubing 61 is inserted through infusion port 16 to facilitate installation of tubing 61. The plug seat 17 further has a first connector 172, a second connector 173, a third connector 174, a gas transmission hole 175 and a gas release valve 176, wherein the first connector 172, the second connector 173 and the third connector 174 respectively pass through the plug seat 17 and extend to the culture chamber 14 and the external space, the gas transmission hole 175 passes through the plug seat 17, preferably, the gas transmission hole 175 has a joint for connecting a pipe body, so that external gas flows through the gas transmission hole into the culture chamber through the pipe body, and the gas release valve 176 is connected to the culture chamber 14 and the external space, so that when the gas pressure in the culture chamber 14 rises, the gas can release to the external space, and the redundant gas can be discharged. The positions of the plug seat 17 and the infusion hole 16, and the positions of the first connector 172, the second connector 173, the third connector 174, the infusion hole 175 and the release valve 176 are only one embodiment of the present invention, and it is feasible to directly mold the infusion hole 16, the first connector 172, the second connector 173, the third connector 174, the infusion hole 175 and the release valve 176 on the bottom wall 11, the side wall 12 or the top cover 13 instead of disposing the plug seat 17 at any edge, middle or center of the bottom wall 11, the side wall 12 or the top cover 13, or omitting the plug seat 17.
The base 20 is accommodated in the culture chamber 14 for accommodating the cell culture bag 90 and driving the cell culture bag 90 to swing, specifically, referring to fig. 1 and 3, the base 20
The rocking device comprises a rocking base 21 and a carrying platform, wherein the rocking base 21 comprises a base 211, one or more driving rods 212 and a pedestal 213, the bottom end of each driving rod 212 is arranged on the base 211, the top end of each driving rod 212 is arranged on the pedestal 213, and the base 211 can respectively drive each driving rod 212 to move up and down, so that the pedestal 213 is partially jacked by the driving rods 212 to tilt and rock. The carrier includes a plate 221 and a partition 222, the plate 221 is disposed on the pedestal 213 and swings with the pedestal, the partition 222 is mounted on the plate 221 to keep a space between the partition 222 and the plate 221, and the partition 222 has a plurality of openings to communicate the space below the partition 222 with other spaces in the growth chamber 14 through the openings.
The temperature control module is at least partially disposed in the culture chamber 14 and is capable of detecting and controlling the temperature of the culture chamber 14, and specifically, the temperature control module includes a plurality of heaters 31 and a plurality of temperature sensors 32, the heaters 31 and the temperature sensors 32 are respectively disposed on the inner surface of the sidewall 12 and electrically connected to the first connector 172 for connection and control of the control module 70 disposed outside the culture chamber 14, the temperature sensors 32 sense the temperature of the culture chamber 14, and the heaters 31 can be driven to start heating or stop heating to maintain the temperature of the culture chamber 14 close to a target temperature suitable for cell culture, such as 37 ℃. It should be noted that, since the target temperature suitable for cell culture is usually higher than room temperature, the temperature control module is usually controlled to start or stop heating the culture chamber 14 for controlling the temperature of the culture chamber 14, but depending on the temperature requirement of the culture chamber, the temperature control module may also comprise a cooler alone or a cooler and a heater.
The carbon dioxide module includes a plurality of carbon dioxide sensors 41, such as infrared spectrum carbon dioxide sensors, disposed on the inner surface of the sidewall 12 and electrically connected to the second connector 173, for sensing the carbon dioxide concentration in the surrounding environment, i.e., the culture chamber 14, and outputting electrical signals.
The carbon dioxide supply source can be controlled to provide carbon dioxide to the culture chamber 14, specifically, the carbon dioxide supply source includes a gas cylinder connector 51, a control valve 52 and a gas supply pipe 53, the gas cylinder connector 51 can be connected to a carbon dioxide high-pressure gas cylinder 91 to receive carbon dioxide from the carbon dioxide high-pressure gas cylinder, the control valve 52 is connected to one end of the gas cylinder connector 51 and one end of the gas supply pipe 53 and can be controlled to open and close and adjust the flow rate of gas supplied from the gas cylinder connector 51 to the gas supply pipe 53, and the other end of the gas supply pipe 53 is connected to the gas transmission hole 175.
The infusion source can be controlled to provide the culture solution to the cell culture bag in the culture chamber 14, specifically, the infusion source includes an infusion tube 61, a storage tank 62 and an infusion pump 63, one end of the infusion tube 61 extends into the culture chamber 14 through the infusion hole 16 and is connected to the cell culture bag 90, the other end is connected to the infusion pump 63, the infusion pump is connected to the storage tank 62 and can be controlled to open and close and regulate the flow rate of the liquid supplied from the storage tank 62 to the infusion tube 61, the storage tank 62 is used for storing the culture solution for cell culture, preferably, the storage tank 62 has a heat insulation foam interlayer or a vacuum interlayer, and the storage tank 62 has a refrigeration device, such as a refrigerant-phase change type refrigeration device or a thermoelectric refrigeration device, so that the temperature of the culture solution suitable for storage can be maintained in the storage tank 62, such as 4 ℃.
The control module 70 is, for example, a programmable system on a chip, and can be programmed to execute the control described later, or can be connected to a computer to acquire instructions and further execute the control. The control module 70 is connected to the first connector 172, and can measure the temperature of the culture chamber 14 through the first connector 172 and the temperature sensor 32, so as to control the on/off of the heater 31 through the first connector 172, so that the temperature of the culture chamber 14 approaches a target temperature suitable for cell culture, for example, 37 ℃. The control module 70 is connected to the second connector 173 and the control valve 52, and can measure the concentration of carbon dioxide in the culture chamber 14 via the second connector 173 and the carbon dioxide sensor 41, so as to control the flow rate of carbon dioxide flowing from the gas supply pipe 53 and the gas transmission hole 175 into the culture chamber 14 via the control valve 52, so that the concentration of carbon dioxide in the culture chamber 14 approaches the concentration of carbon dioxide suitable for cell culture, for example, 5% by volume. The control module 70 is connected to the infusion pump 63, and can control the flow rate of the culture solution supplied to the infusion tube 61 and the cell culture bag 90 via the infusion pump 63, and can set the supply flow rate by a program, for example, a fixed-time and fixed-amount supply or a predetermined flow rate depending on the time length elapsed after the start of cell culture. The control module 70 may further connect to the third connector 174, and connect to the base 20 through the third connector 174, and supply power to the rocking base 21 and control the opening and closing of the rocking base 21, the rocking frequency and the amplitude of the rocking vibration, but if the third connector is omitted, the rocking base 21 is driven by a built-in battery, which is not necessary.
By using the above-mentioned automatic cell culture apparatus, when a user wants to perform cell culture, each component can be configured in advance, the culture solution is stored in the storage tank 62, the gas cylinder connector 51 is connected with the carbon dioxide high-pressure gas cylinder 91, and water is poured into the tray body of the base, the cell culture bag 90 to be performed cell culture can be connected with the infusion tube 61 and then placed into the culture chamber 14, the cell culture bag 90 is placed on the partition board, the partition board can separate the upper cell culture bag 90 from the lower water, the infusion tube 61 can be pressed into the infusion hole through the cut of the plug seat 17, the observation board 15 and the top cover 13 can be covered, and the installation is completed.
In the process of executing cell culture, the flow rate of the culture solution and the flow rate of carbon dioxide can be controlled by the control module, and water in the base plate body can be evaporated and spread in the culture chamber through the open pores of the partition plate, so that the temperature, the humidity and the concentration of the carbon dioxide in the culture chamber are kept constant. Wherein, in order to avoid the phenomenon of local non-uniformity of temperature, humidity and carbon dioxide concentration in the culture chamber, a fan can be additionally arranged in the culture chamber to promote the homogenization of the environment in the culture chamber; in order to record the environmental conditions during the cell culture process, a humidity sensor may be disposed in the culture chamber and electrically connected to the control device 70 via a connector for the control device to measure the humidity in the culture chamber.
When the automatic cell culture device is arranged, because the body, the carbon dioxide supply source and the infusion source are separately arranged, a user can also add a plurality of groups of infusion pumps, infusion pipes, control valves and air supply pipes by using the storage tank of the same infusion source and the same carbon dioxide high-pressure air cylinder, connect a plurality of groups of bodies for use, modularly configure the bodies, stack and store the bodies, and have the characteristics of simple structure, low setting cost and high space utilization rate.
Secondly, the automatic cell culture device has simple body structure, is convenient to install and set, is easy to repeatedly disinfect and use, and has the characteristic of easy operation.
In the above embodiment, the control device has a single housing, which is convenient for controlling multiple sets of infusion pumps, control valves and main bodies with a single controller, please refer to fig. 4, in another embodiment of the present invention, the control device 70 may be fixed to the main body 10 instead, and a control device 70 may be used with a main body 10, or a control device 70 may be used with a main body 10 or other main bodies without a control device, and the similar usage and features as described above are still provided
In summary, the automated cell culture device of the present invention modularizes each component, simplifies the structure, and is easy to use, move, configure quickly, and reduce the cost, and the space utilization rate can be improved by stacking and storing, thereby further reducing the cost.
Claims (10)
1. An automated cell culture apparatus for placing a cell culture bag and performing cell culture, comprising:
the cell culture bag comprises a body, wherein the body is provided with a culture chamber and a transfusion hole, the transfusion hole is communicated with the culture chamber and the external space, so that when the cell culture bag is placed in the culture chamber, a transfusion pipe can be connected between a transfusion source outside the body and the cell culture bag in the culture chamber through the transfusion hole.
2. The automated cell culture apparatus of claim 1, wherein: the cell culture bag is arranged on the carrying platform, and the cell culture bag is arranged on the carrying platform.
3. The automated cell culture apparatus of claim 2, wherein: the carrying platform comprises a plate body and a partition plate, wherein the plate body is used for bearing water, the partition plate is used for bearing the cell culture bag, the partition plate is arranged on the plate body in a supporting mode to separate the water borne in the plate body from the cell culture bag, and the partition plate is provided with a plurality of openings so that the water borne in the plate body can be evaporated and dispersed in the culture chamber.
4. The automated cell culture apparatus of claim 1, wherein: the body is provided with a first connector, the heater and the temperature sensor are electrically connected with the first connector, and the first connector is exposed from the surface of the body.
5. The automated cell culture apparatus of claim 4, wherein: the body is provided with a plug seat, the plug seat extends to the external space from the culture chamber, the infusion hole penetrates through and is formed in the plug seat, and the first connector is arranged on the plug seat.
6. The automated cell culture apparatus of any one of claims 1 to 4, wherein: the carbon dioxide module comprises a carbon dioxide sensor which is positioned in the culture chamber, the body is provided with a second connector and a gas transmission hole, the carbon dioxide sensor is electrically connected with the second connector, the second connector is exposed from the surface of the body, and the gas transmission hole is communicated with the culture chamber and the external space so as to be connected with a carbon dioxide supply source outside the body.
7. The automated cell culture apparatus of claim 5, wherein: the carbon dioxide module comprises a carbon dioxide sensor which is positioned in the culture chamber, the body is provided with a second connector and a gas transmission hole, the carbon dioxide sensor is electrically connected with the second connector, the second connector is exposed from the surface of the body, and the gas transmission hole is communicated with the culture chamber and the external space so as to be connected with a carbon dioxide supply source outside the body.
8. The automated cell culture apparatus of claim 7, wherein: the second connector is arranged on the plug seat.
9. The automated cell culture apparatus of any one of claims 1 to 5, wherein: the first connector is electrically connected with the control module, the control module detects the temperature of the culture chamber through the temperature sensor, and the control module controls the heater to be opened and closed to enable the temperature of the culture chamber to approach 37 ℃.
10. An automated cell culture apparatus according to claim 7 or 8, wherein: the culture chamber also comprises a control module, the second connector is electrically connected with the control module, the control module detects the concentration of carbon dioxide in the culture chamber through the carbon dioxide module, and the control module controls the carbon dioxide supply source to supply carbon dioxide to the culture chamber through the gas transmission hole, so that the volume concentration of the carbon dioxide in the culture chamber approaches to 5%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010479892.3A CN113736651A (en) | 2020-05-29 | 2020-05-29 | Automatic cell culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010479892.3A CN113736651A (en) | 2020-05-29 | 2020-05-29 | Automatic cell culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023116833A1 (en) * | 2021-12-22 | 2023-06-29 | 基亚生物科技股份有限公司 | Integrated automated cell culture device |
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| TW201713765A (en) * | 2015-10-02 | 2017-04-16 | Lun-Kuang Liu | Cell incubator which is characterized by the small overall size and lightweight appearance, to realize automated cell cultivation |
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