CN113604332B - Disposable anaerobic environment cell culture bag - Google Patents
Disposable anaerobic environment cell culture bag Download PDFInfo
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- CN113604332B CN113604332B CN202110818275.6A CN202110818275A CN113604332B CN 113604332 B CN113604332 B CN 113604332B CN 202110818275 A CN202110818275 A CN 202110818275A CN 113604332 B CN113604332 B CN 113604332B
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- base
- cover body
- filter tip
- disposable
- cell culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a disposable anaerobic environment cell culture bag which comprises a base, wherein a cover body is arranged on the upper side of the base in a separating mode, the cover body is made of disposable soft rubber materials, the cover body is connected and fixed with the base through a clamp, the lower side face of the base is concave, circular bosses penetrating through the bottom face are arranged at the center of the bottom face and the positions of the periphery of the bottom face, and through holes penetrating through the circular bosses are formed in the circular bosses. According to the invention, the film is paved on the base to be in contact with the inner edge table on the cover body, and the disposable soft rubber of the cover body is arranged, so that a double sealing effect is formed when the base and the cover body are inflated, the high-temperature sterilization operation of the cover body is avoided, the pollution risk is reduced, and the operation process is simplified; the first filter tip communicated through the first communication pipe inflates the cavity at the middle part, then the cover body is jacked up, and simultaneously, the gas is discharged through the peripheral second filter tip, so that the inflation process is visual and controllable, and the operation is convenient.
Description
Technical Field
The invention relates to the field of test instruments, in particular to a disposable anaerobic environment cell culture bag.
Background
The hypoxia incubator is a self-sufficient sealed chamber and is suitable for the existing laboratory incubators. Each cell is sealed independently with portions of the O-ring being uniformly compressed by the ring clamp, thereby forming a reliable airtight chamber. The cylindrical wall and hemispherical top and bottom minimize air flow resistance, eliminating dead zones during purging. When the sealed incubator is used, the sealed incubator is inflated through the air bottle and the matched flowmeter, and then the sealed incubator is placed in a normal incubator, but the current cover body is molded, high-temperature disinfection is needed after the sealed incubator is used, the sealed incubator is repeatedly used, the risk of pollution is easily caused, in addition, the air inlet and the air outlet are both arranged at the bottom when the sealed incubator is inflated, the inflation capacity is not conveniently confirmed when the air exchange is performed, and the air content in the sealed space is not well controlled.
Disclosure of Invention
The present invention aims to solve the above problems by providing a disposable anaerobic environment cell culture bag.
The invention realizes the above purpose through the following technical scheme:
the utility model provides a disposable hypoxia environment cell culture bag, includes the base, and the separation sets up the lid of base upside, the lid is disposable soft rubber material, the lid with it is fixed through the clamp connection between the base, the base downside is the concavity, and bottom surface center and week circle position are provided with the circular boss that passes this bottom surface, and this circular boss inside is provided with the through-hole that runs through, the base center circular boss upper end is provided with first filter tip, lower extreme is connected with first communicating pipe, the base week circle circular boss upper end is provided with second filter tip, the lower extreme is provided with the second communicating pipe, first communicating pipe all be provided with the pipe clamp on the second communicating pipe external diameter, the base with be provided with the sealing washer between the contact surface about the lid, be provided with disposable film on the upper surface of base.
Further set up: the base is circular, and the outer diameter circumference of the upper end is provided with a flange, and the upper surface of the flange is provided with a first groove.
So set up, make the base supports the household utensils box and places, first recess is right the sealing washer is spacing fixed.
Further set up: the upper side of the cover body is hemispherical, an inner edge table is arranged on the inner side of the lower end face of the cover body, and an outer edge table is arranged on the lower side of the outer surface of the cover body.
The arrangement is that the outer edge table and the inner edge table are respectively sealed with the film and the flange, so that the first communication pipe is convenient for introducing gas into the sealing cavity formed by the cover body and the base after being buckled, and the gas diffuses from the top to the periphery and props up the cover body.
Further set up: the cross section of the clamp is concave, and the clamp is in interference connection with an outer edge table on the cover body and a flange on the base.
The arrangement is that the clamp is convenient for buckling the base and the cover together to form a sealed cavity.
Further set up: the sealing ring is in interference connection with the first groove of the upper flange of the base and the upper outer edge table of the cover body.
The sealing ring is used for sealing connection between the base and the cover.
Further set up: the circular boss is in the base border is provided with everywhere, first filter tip the second filter tip respectively with circular boss upper end threaded connection, first filter tip the second filter tip inboard is provided with the filter screen.
So set up, be convenient for first filter tip the second filter tip is in connect fixedly on the circular boss, filter gas when giving vent to anger simultaneously.
Further set up: the film is paved on the upper end face of the base, and the film is provided with a through hole through which the first filter tip and the second filter tip pass.
So set up, through the laying of film improves the base with the leakproofness of the inner edge platform of lid makes the household utensils box of base and inner chamber carry out the diaphragm processing simultaneously, avoids the pollution in the cavity.
Further set up: the second communicating pipe is positioned at the lower end of the base and is in an annular shape, and a straight pipe connected with the circular boss on the lower bottom surface of the base is arranged at the upper end of the second communicating pipe.
So set up, be convenient for the second communicating pipe with the circular boss intercommunication of base border carries out the air inlet and outlet operation to sealed cavity.
Further set up: the upper end face of the base is positioned between the circular bosses and is provided with a vessel box.
So set up, place the cell to be cultivateed in the household utensils box, improve the utilization ratio of operating space.
Further set up: the height of the first filter tip is close to the top of the cover body, the height of the first filter tip is larger than that of the second filter tip, and the outer diameters of the film and the cover body are larger than that of the base.
So set up, make the lid is sealed back accessible second filter tip or first filter tip is got into the cavity and is selected different gases, and can fill the cavity fast, makes the film the lid is external pass through the clamp seals both.
Compared with the prior art, the invention has the following beneficial effects:
by paving the film on the base to be in contact with the inner edge table on the cover body and setting the cover body with disposable soft rubber, a double sealing effect is formed when the base and the cover body are inflated, so that the high-temperature sterilization operation of the cover body is avoided, the risk of infection is reduced, and the operation process is simplified; the first filter tip communicated through the first communication pipe inflates the cavity at the middle part, then the cover body is jacked up, and simultaneously, the gas is discharged through the peripheral second filter tip, so that the inflation process is visual and controllable, and the operation is convenient.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions of the prior art, the drawings that are necessary for the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention and that other drawings can be obtained according to these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a schematic view of a first construction of a disposable anaerobic environment cell culture bag according to the present invention;
FIG. 2 is a schematic diagram of a second construction of the disposable anaerobic environment cell culture bag according to the present invention;
FIG. 3 is a first schematic view of the explosive structure of the disposable anaerobic environment cell culture bag according to the present invention;
FIG. 4 is a second schematic view of the explosive structure of the disposable hypoxic environment cell culture bag of the invention;
FIG. 5 is a schematic top view of a disposable anaerobic environment cell culture bag according to the present invention;
FIG. 6 is a schematic view of the cross-sectional A-A configuration of FIG. 5;
FIG. 7 is a schematic view of the partially enlarged structure of FIG. 6;
FIG. 8 is a schematic diagram of the structure of a disposable anaerobic environment cell culture bag according to the present invention;
FIG. 9 is a schematic view showing the structure of the disposable anaerobic environment cell culture bag according to the present invention in a container box placement state.
The reference numerals are explained as follows:
1. a base; 101. a flange; 102. a first groove; 2. a cover body; 201. an inner edge table; 202. an outer edge table; 3. a clamp; 4. a first communication pipe; 5. a second communicating pipe; 6. a pipe clamp; 7. a seal ring; 8. a film; 9. a first filter; 10. a second filter; 11. a circular boss; 12. a vessel box.
Detailed Description
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art in a specific case.
The invention is further described below with reference to the accompanying drawings:
as shown in fig. 1-9, the disposable anaerobic environment cell culture bag comprises a base 1, a cover body 2 is arranged on the upper side of the base 1 in a separating way, the cover body 2 is made of disposable soft rubber materials, the cover body 2 and the base 1 are connected and fixed through a clamp 3, the lower side surface of the base 1 is concave, the center of the bottom surface and the periphery are provided with round bosses 11 penetrating through the bottom surface, through holes penetrating through the round bosses 11 are formed in the round bosses, the connection and the fixation with a first communicating pipe 4, a second communicating pipe 5, a first filter tip 9 and a second filter tip 10 are conveniently carried out, the first filter tip 9 is arranged at the upper end of the round boss 11 in the center of the base 1, the first communicating pipe 4 is connected with the first communicating pipe 4, the second filter tip 10 is arranged at the lower end of the round boss 11 in the periphery of the base 1, the clamp 6 is arranged on the outer diameter of the first communicating pipe 4 and the second communicating pipe 5, a sealing ring 7 is arranged between the upper contact surface and the lower contact surface of the base 1 and the cover body 2, and a disposable film 8 is arranged on the upper surface of the base 1.
Preferably: the base 1 is circular, a flange 101 is arranged on the outer diameter periphery of the upper end, a first groove 102 is arranged on the upper surface of the flange 101, so that the base 1 supports and places the vessel box 12, and the first groove 102 limits and fixes the sealing ring 7; the upper side surface of the cover body 2 is hemispherical, the inner side of the lower end surface of the cover body 2 is provided with an inner edge table 201, the lower side of the outer surface of the cover body 2 is provided with an outer edge table 202, so that the outer edge table 202 and the inner edge table 201 respectively form sealing with the film 8 and the flange 101, the first communication pipe 4 is convenient for introducing gas into a sealing cavity formed by buckling the cover body 2 and the base 1, the gas diffuses from the top to the periphery, and the cover body 2 is supported; the section of the clamp 3 is concave, and the clamp 3 is in interference connection with an outer edge table 202 on the cover body 2 and a flange 101 on the base 1, so that the clamp 3 is convenient for buckling the base 1 and the cover body 2 together to form a sealed cavity; the sealing ring 7 is in interference connection with the first groove 102 of the upper flange 101 of the base 1 and the upper outer edge table 202 of the cover body 2, and the base 1 and the cover body 2 are in sealing connection through the sealing ring 7; the round boss 11 is arranged around the base 1, the first filter tip 9 and the second filter tip 10 are respectively in threaded connection with the upper end of the round boss 11, and the filter screens are arranged on the inner sides of the first filter tip 9 and the second filter tip 10, so that the connection and fixation of the first filter tip 9 and the second filter tip 10 on the round boss 11 are facilitated, and meanwhile, gas is filtered during gas inlet and outlet; the film 8 is paved on the upper end surface of the base 1, the film 8 is provided with through holes for the first filter tip 9 and the second filter tip 10 to pass through, the tightness of the inner edge table 201 of the base 1 and the cover body 2 is improved through the paving of the film 8, and meanwhile, the base 1 and the vessel box 12 of the inner cavity are subjected to diaphragm treatment, so that the pollution in the cavity is avoided; the second communicating pipe 5 is positioned at the lower end of the base 1 and is in an annular shape, and a straight pipe connected with a circular boss 11 on the lower bottom surface of the base 1 is arranged at the upper end, so that the second communicating pipe 5 is conveniently communicated with the circular boss 11 on the periphery of the base 1, and air inlet and outlet operations are carried out on the sealed cavity; the upper end face of the base 1 is provided with the vessel box 12 between the round bosses 11, the vessel box 12 is provided with three parts at the inner side, and three layers can be arranged, so that cells to be cultured are placed in the vessel box 12, and the utilization rate of an operation space is improved; the height of the first filter tip 9 is close to the top of the cover body 2, the height of the first filter tip 9 is larger than that of the second filter tip 10, the outer diameters of the film 8 and the cover body 2 are larger than that of the base 1, different gases can enter the cavity through the second filter tip 10 or the first filter tip 9 after the cover body 2 is sealed, the cavity can be quickly filled, and the film 8 and the outer edge of the cover body 2 are sealed through the clamp 3.
The working principle and the use flow of the invention are as follows: the disposable film 8 is paved on the upper end face of the base 1, the vessel box 12 with cells placed thereon is placed on the film 8, the cover body 2 is placed on the upper end of the base 1, the inner edge table 201 of the cover body 2 is pressed on the film 8, the outer edge of the film 8 and the outer edge table 202 are buckled on the sealing ring 7 of the flange 101 of the periphery of the base 1, the outer edge table 202, the flange 101 and the film 8 are buckled and fixed through the clamp 3, the first connecting pipe 4 is connected with an air inlet source, the air source continuously enters into a cavity formed by the base 1 and the cover body 2, the air continuously upwards props up the cover body 2 in the middle part, and then diffuses all around along the inner wall of the cover body 2 after reaching the top of the cover body 2, the air is outwards discharged through the second filter tip 10 on the periphery, or the air is connected with the air inlet source through the second connecting pipe 5, the air is inflated into the cavity through the second filter tip 10, the air is outwards discharged through the first filter tip 9 until the cover body 2 is completely jacked up to be round, and the clamp 6 is buckled.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims.
Claims (5)
1. The utility model provides a disposable oxygen deficiency environment cell culture bag, includes base (1), its characterized in that: the separation sets up lid (2) of base (1) upside, lid (2) are disposable soft rubber material, lid (2) with connect fixedly through clamp (3) between base (1), base (1) downside is the concavity, and bottom surface center and border position are provided with round boss (11) that pass this bottom surface, this round boss (11) inside is provided with the through-hole that runs through, base (1) center round boss (11) upper end is provided with first filter tip (9), the lower extreme is connected with first communicating tube (4), base (1) border round boss (11) upper end is provided with second filter tip (10), the lower extreme is provided with second communicating tube (5), all be provided with pipe clamp (6) on the second communicating tube (5) external diameter, base (1) with be provided with sealing washer (7) between the lower contact surface on lid (2), be provided with on the upper surface of base (1) first filter tip (8), be provided with on the surface of one side of base (1) flange (101) in the shape, be provided with one side flange (101) on the side of being in the semicircle, base (1) is provided with one side surface of the recess (101), the novel filter is characterized in that an outer edge table (202) is arranged on the lower side of the outer surface of the cover body (2), the cross section of the clamp (3) is concave, the clamp (3) is in interference connection with the outer edge table (202) on the cover body (2) and the flange (101) on the base (1), the sealing ring (7) is in interference connection with the first groove (102) of the flange (101) on the base (1), the outer edge table (202) on the cover body (2) is in interference connection, the film (8) is paved on the upper end face of the base (1), and the through hole through which the first filter tip (9) and the second filter tip (10) penetrate is formed in the film (8).
2. The disposable hypoxic environment cell culture bag of claim 1, wherein: the round boss (11) is arranged around the base (1), the first filter tip (9) and the second filter tip (10) are respectively connected with the upper end of the round boss (11) in a threaded mode, and a filter screen is arranged on the inner side of the first filter tip (9) and the inner side of the second filter tip (10).
3. The disposable hypoxic environment cell culture bag of claim 1, wherein: the second communicating pipe (5) is positioned at the lower end of the base (1) and is in an annular shape, and a straight pipe connected with the circular boss (11) on the lower bottom surface of the base (1) is arranged at the upper end of the second communicating pipe.
4. The disposable hypoxic environment cell culture bag of claim 1, wherein: a vessel box (12) is arranged between the round bosses (11) on the upper end face of the base (1).
5. The disposable hypoxic environment cell culture bag of claim 1, wherein: the height of the first filter tip (9) is close to the top of the cover body (2), the height of the first filter tip (9) is larger than that of the second filter tip (10), and the outer diameter of the film (8) and the cover body (2) is larger than that of the base (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110818275.6A CN113604332B (en) | 2021-07-20 | 2021-07-20 | Disposable anaerobic environment cell culture bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110818275.6A CN113604332B (en) | 2021-07-20 | 2021-07-20 | Disposable anaerobic environment cell culture bag |
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| Publication Number | Publication Date |
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| CN113604332A CN113604332A (en) | 2021-11-05 |
| CN113604332B true CN113604332B (en) | 2023-09-19 |
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| CN202110818275.6A Active CN113604332B (en) | 2021-07-20 | 2021-07-20 | Disposable anaerobic environment cell culture bag |
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Citations (7)
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| CN206457493U (en) * | 2017-02-16 | 2017-09-01 | 沈建 | A kind of pocket type anoxic box |
| CN207259522U (en) * | 2017-08-31 | 2018-04-20 | 张曦然 | Tissue Culture Dish for adherent cell growth |
| EP3719111A1 (en) * | 2019-04-04 | 2020-10-07 | Universidad de Navarra | Devices for cellularized membrane cultivation and kits |
| CN211771359U (en) * | 2019-12-19 | 2020-10-27 | 川北医学院附属医院 | Simple and easy cell oxygen deficiency culture apparatus |
| CN212581897U (en) * | 2020-05-28 | 2021-02-23 | 航天中心医院 | Cell culture dish closing device |
| CN213327660U (en) * | 2020-09-18 | 2021-06-01 | 瑞诺威德(杭州)医学科技有限公司 | Dual-purpose cell culture dish |
| CN213506979U (en) * | 2020-09-22 | 2021-06-22 | 中国人民解放军空军军医大学 | Simple and easy cell oxygen deficiency culture apparatus |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11078455B2 (en) * | 2014-06-09 | 2021-08-03 | Seiichi YOKOO | Closed culture vessel for adherent cells |
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2021
- 2021-07-20 CN CN202110818275.6A patent/CN113604332B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206457493U (en) * | 2017-02-16 | 2017-09-01 | 沈建 | A kind of pocket type anoxic box |
| CN207259522U (en) * | 2017-08-31 | 2018-04-20 | 张曦然 | Tissue Culture Dish for adherent cell growth |
| EP3719111A1 (en) * | 2019-04-04 | 2020-10-07 | Universidad de Navarra | Devices for cellularized membrane cultivation and kits |
| CN211771359U (en) * | 2019-12-19 | 2020-10-27 | 川北医学院附属医院 | Simple and easy cell oxygen deficiency culture apparatus |
| CN212581897U (en) * | 2020-05-28 | 2021-02-23 | 航天中心医院 | Cell culture dish closing device |
| CN213327660U (en) * | 2020-09-18 | 2021-06-01 | 瑞诺威德(杭州)医学科技有限公司 | Dual-purpose cell culture dish |
| CN213506979U (en) * | 2020-09-22 | 2021-06-22 | 中国人民解放军空军军医大学 | Simple and easy cell oxygen deficiency culture apparatus |
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| CN113604332A (en) | 2021-11-05 |
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