CN217895599U

CN217895599U - Can airtight large capacity cell culture bottle

Info

Publication number: CN217895599U
Application number: CN202221045548.4U
Authority: CN
Inventors: 钱杨华
Original assignee: Shanghai Jinyu Biotechnology Co ltd
Current assignee: Shanghai Jinyu Biotechnology Co ltd
Priority date: 2022-05-05
Filing date: 2022-05-05
Publication date: 2022-11-25
Anticipated expiration: 2032-05-05

Abstract

The utility model relates to the field of cell culture bottles, in particular to a sealable large-capacity cell culture bottle, which comprises a culture bottle body, wherein the top of the culture bottle body is provided with an upper bottle opening, the upper bottle opening is provided with a bottle cap, the bottle cap is provided with an upper air hole, the bottom of the upper air hole is provided with an upper air film, and an upper sealing plug is arranged in the upper air hole; the bottom of blake bottle body is installed down the ventilated membrane, and the supporting seat is installed to the bottom of lower ventilated membrane, and lower bleeder vent has been seted up at the middle part of supporting seat, and the stopper is equipped with the lower sealing plug in the bleeder vent. The utility model discloses in, the blake bottle is when cultivateing, and ventilated membrane, lower ventilated membrane take a breath in the accessible, and the mode of taking a breath of ventilated membrane on only leaning on the bottle lid than traditional provides better ventilation effect, the amplification efficiency when can greatly improve cell culture.

Description

Can airtight large capacity cell culture bottle

Technical Field

The utility model relates to a cell culture bottle technical field especially relates to a can airtight large capacity cell culture bottle.

Background

In the production process of many biological medicines, especially cell medicines, cells often need to be cultured, when culturing the cells, various culture bottles can be used, at present, the culture bottles on the market are in the cell culture process, because only a ventilating port on the bottle cap can be used, the efficiency of gas exchange carried out by the cells is poor, the density of the carried cells is low, the height of the culture solution which can be contained once is low, the solution often needs to be changed, and because the ventilating port on the culture bottle cap can not be closed, the culture bottles can not carry out effective surface sterilization before being transmitted into the aseptic operation environment, and the pollution risk in the operation process of the culture bottles is increased.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a cell culture bottle with high sealing capacity.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a sealable high-capacity cell culture bottle comprises a culture bottle body, wherein an upper bottle opening is formed in the top of the culture bottle body, a bottle cap is mounted on the upper bottle opening, an upper air hole is formed in the bottle cap, an upper air-permeable film is mounted at the bottom of the upper air hole, and an upper sealing plug is arranged in the upper air hole;

lower ventilated membrane is installed to the bottom of blake bottle body, and the supporting seat is installed to the bottom of lower ventilated membrane, and bleeder vent has been seted up down at the middle part of supporting seat, and the stopper is equipped with lower sealing plug in the bleeder vent down.

Preferably, the bottom of the supporting seat is provided with a supporting frame for supporting the culture bottle.

Preferably, the volume of the culture bottle body is 100ml-5000ml, and the shape of the culture bottle body is round or square.

Preferably, the upper breathable film and the lower breathable film adopt waterproof breathable films capable of blocking microorganisms.

Preferably, the upper sealing plug and the lower sealing plug are made of silica gel materials, and the upper sealing plug and the lower sealing plug are tightly sealed with the upper air hole and the lower air hole respectively.

The utility model has the advantages that:

1. the utility model discloses in, the blake bottle is when cultivateing, and ventilated membrane, lower ventilated membrane take a breath in the accessible, and the mode of taking a breath of ventilated membrane on only leaning on the bottle lid than traditional provides better ventilation effect, the amplification efficiency when can greatly improve cell culture.

2. The utility model discloses in, owing to increased down the ventilated membrane and take a breath, provide the ventilation effect, make the blake bottle when cultivateing, can once only add more culture solutions, reduce the liquid change number of times of cultivateing the in-process, can effectively reduce personnel's operating strength and the pollution risk that frequent operation brought.

3. The utility model discloses in, the blake bottle is when needs trade liquid or sample, results operation, can seal through upper and lower sealing plug, bleeder vent from top to bottom, and the blake bottle just can form a totally airtight container like this, can carry out surface sterilization back again and pass into aseptic operating environment such as isolator in, and can not destroy aseptic environment.

Drawings

FIG. 1 is a schematic view of a high capacity cell culture bottle capable of being sealed according to the present invention;

FIG. 2 is a schematic view of the structure of the airtight cell culture bottle when the air holes are opened.

In the figure: 1. a culture bottle body; 2. putting the bottle mouth on the bottle; 3. a bottle cap; 4. air holes are arranged; 5. an upper breathable film; 6. an upper sealing plug; 7. a lower breathable film; 8. a supporting base; 9. air holes are formed; 10. A support frame; 11. and a lower sealing plug.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-2, a sealable high-capacity cell culture bottle comprises a culture bottle body 1, wherein an upper bottle opening 2 is formed in the top of the culture bottle body 1, a bottle cap 3 is mounted on the upper bottle opening 2, an upper air vent 4 is formed in the bottle cap 3, an upper air-permeable film 5 is mounted at the bottom of the upper air vent 4, and an upper sealing plug 6 is arranged in the upper air vent 4 in a plugging manner;

lower ventilated membrane 7 is installed to the bottom of blake bottle body 1, and supporting seat 8 is installed to the bottom of lower ventilated membrane 7, and bleeder vent 9 has been seted up down at the middle part of supporting seat 8, and lower bleeder vent 9 inside plug is equipped with lower sealing plug 11, and wherein the support frame 10 that is used for supporting the blake bottle is installed to the bottom of supporting seat 8.

Furthermore, the volume of the culture bottle body 1 is 100ml-5000ml, and the shape of the culture bottle body 1 is round or square.

Moreover, the upper breathable film 5 and the lower breathable film 7 adopt waterproof breathable films capable of blocking microorganisms, and the waterproof breathable films capable of blocking microorganisms are the prior art and can be directly purchased in the market.

And, go up sealing plug 6 and lower sealing plug 11 and all adopt the silica gel material, and go up sealing plug 6, lower sealing plug 11 and tightly seal to going up bleeder vent 4, lower bleeder vent 9 stopper respectively.

In this embodiment, as shown in fig. 1, the upper mouth 2 and the cap 3 of the culture bottle are connected by screw threads, the upper edges of the cap 3 and the upper mouth 2 are designed to be sealed, and when the screw threads are screwed, the contact surface can be completely sealed. The bottom of blake bottle body 1 is provided with the round flange, is connected with lower ventilated membrane 7, supporting seat 8 through the flange, then realizes the chucking through the buckle on the support frame 10 and seals.

When in use, when the upper air hole 4 of the bottle cap 3 is plugged with the upper sealing plug 6 and the lower air hole 9 is provided with the lower sealing plug 11, the interior of the culture bottle can be completely sealed; when the upper sealing plug 6 and the lower sealing plug 11 are both pulled out, a relatively closed environment suitable for culturing cells is formed inside the culture bottle, and air exchange can be carried out only through the upper breathable film 5 or the lower breathable film 7, and because the upper breathable film 5 or the lower breathable film 7 adopts the waterproof breathable film capable of filtering microorganisms, the inside of the culture bottle can be ensured not to be polluted during culture.

In this example, the practical procedure of using the culture flask is as follows: when the cell is subjected to aseptic culture, the culture bottle assembly with the external package and subjected to integral sterilization is placed into an aseptic environment of operation, then the external package is subjected to surface sterilization, the external package is opened, the bottle cap 3 is unscrewed, the cell to be cultured is inoculated into the culture bottle from the upper bottle mouth 2, then the culture solution is added, the bottle cap 2 is screwed, and then the upper sealing plug 6 and the lower sealing plug 11 are pulled out, so that the culture box can be placed into the culture box for culture.

As shown in FIG. 2, during the culture process, the upper and lower sealing plugs 6 and 11 are pulled out, so that the culture bottle is ventilated through the upper and

lower ventilation holes

4 and 7 and the lower ventilation hole 9 to maintain the oxygen content required for cell culture.

When the culture solution inside the culture bottle needs to be changed or sampled in the culture process, as shown in fig. 1, the upper sealing plug 6 and the lower sealing plug 11 are reinstalled, so that the culture bottle temporarily forms a completely closed environment, the outer surface of the culture bottle can be sterilized through the transfer window, then the culture bottle is transferred into the sterile operation environment, and then the bottle cap is opened to change the culture solution and sample. After the operation, the upper sealing plug 6 and the lower sealing plug 11 can be pulled out again and put into the incubator for continuous culture.

Similarly, after the cell culture reaches the required amount, the operation can be repeated, and the cell culture is transferred into a sterile operation environment for harvesting and subpackaging to complete the cell culture of one batch.

In the process of cultivation, because the blake bottle all can carry out gas exchange from top to bottom, increased the efficiency of taking a breath, make the blake bottle can once only hold more culture solution than traditional blake bottle, reduced and traded the liquid number of times, can effectively reduce operating strength and aseptic risk.

Sealing plugs can be arranged on the upper air hole and the lower air hole of the culture bottle to seal, so that after surface sterilization can be realized, the sterile environment is transmitted in a sterile manner, and the risk that the sterile environment is damaged when the traditional culture bottle is transmitted into the sterile environment because the traditional culture bottle cannot be completely sealed due to the air holes and cannot be subjected to surface complete sterilization is avoided.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A sealable high-capacity cell culture bottle comprises a culture bottle body (1), and is characterized in that an upper bottle opening (2) is arranged at the top of the culture bottle body (1), a bottle cap (3) is installed on the upper bottle opening (2), an upper air vent (4) is formed in the bottle cap (3), an upper air permeable membrane (5) is installed at the bottom of the upper air vent (4), and an upper sealing plug (6) is arranged in the upper air vent (4) in a plugging manner;

lower ventilated membrane (7) is installed to the bottom of blake bottle body (1), and supporting seat (8) are installed to the bottom of lower ventilated membrane (7), and bleeder vent (9) have been seted up down at the middle part of supporting seat (8), and bleeder vent (9) internal stopper is equipped with down sealing plug (11) down.

2. A sealable large capacity cell culture flask according to claim 1, wherein the bottom of the support base (8) is provided with a support frame (10) for supporting the flask.

3. A sealable large capacity cell culture flask according to claim 1, wherein the volume of the flask body (1) is 100ml-5000ml, and the shape of the flask body (1) is circular or square.

4. The sealable large-capacity cell culture flask according to claim 1, wherein the upper and lower gas-permeable membranes (5, 7) are water-proof gas-permeable membranes capable of blocking microorganisms.

5. The sealable high-capacity cell culture bottle according to claim 1, wherein the upper sealing plug (6) and the lower sealing plug (11) are made of silica gel, and the upper sealing plug (6) and the lower sealing plug (11) are tightly sealed with the upper vent hole (4) and the lower vent hole (9), respectively.