CN212925054U

CN212925054U - Device for culturing primary stem cells by tissue block method

Info

Publication number: CN212925054U
Application number: CN202021418199.7U
Authority: CN
Inventors: 李炳根; 袁牧
Original assignee: Weihai Jiansheng Biotechnology Co ltd
Current assignee: Weihai Jiansheng Biotechnology Co ltd
Priority date: 2020-07-17
Filing date: 2020-07-17
Publication date: 2021-04-09
Anticipated expiration: 2030-07-17

Abstract

The utility model belongs to the field of cell culture, and particularly discloses a device for culturing primary stem cells by a tissue block method, which comprises a culture cylinder and culture dishes stacked in the culture cylinder, wherein a support plate is arranged in the culture cylinder, pull rods are arranged at two ends of the support plate, a sealing cover is sleeved at the upper end of the culture cylinder, and a first breathable film is covered in a breathable opening in the middle of the sealing cover; a strip-shaped air-permeable groove is formed in the side face of the culture dish, and a second air-permeable film is covered outside the strip-shaped air-permeable groove; the top surface of the dish cover is provided with a positioning ring, and the bottom surface of the culture dish is provided with a positioning lantern ring. The utility model is convenient for culturing multiple groups of stem cells, and the positioning lantern ring on the bottom surface of the culture dish is sleeved on the positioning ring on the dish cover, so as to ensure the stability of stacking; the supporting plate is used for integrally conveying the stacked culture dishes into the culture cylinder or integrally taking the stacked culture dishes out of the culture cylinder, so that the operation is convenient; first ventilated membrane and second ventilated membrane are PTFE group and organize the membrane, under the condition of guaranteeing the air exchange, can avoid bacterium and virus to get into culture apparatus in, prevent to pollute.

Description

Device for culturing primary stem cells by tissue block method

Technical Field

The utility model relates to a cell culture field specifically is a device of original stem cell is cultivateed to organize block method.

Background

Stem cells are a type of pluripotent cells with the ability to self-replicate, and under certain conditions, can differentiate into a variety of functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. The stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent stem cells. Stem cells have the potential function of regenerating various tissues, organs and human bodies, and are called universal cells in the medical field.

At present, a tissue block method is mostly adopted to culture primary stem cells, and tissues are cut into 1mm³And transferring the tissue blocks into a culture bottle at certain intervals, slightly turning over the culture bottle, adding a proper amount of culture solution to a non-cell growth surface, and amplifying the primary stem cells. The above method can only utilize the space at the middle lower part of the bottle body, the space utilization rate is not high, and in the culture process, a plurality of groups of stem cells are required to be cultured simultaneously or a plurality of groups of controls are arranged, so that more culture bottles are occupied, and the controls are not easy to operate; meanwhile, the stem cell culture needs to ensure the air pressure balance while ensuring the sterile state, and bacteria are easily introduced into a general culture medium in the ventilation process to cause pollution.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a organize device of block method culture primary stem cell to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a device for culturing primary stem cells by a tissue block method comprises a culture cylinder and culture dishes stacked in the culture cylinder, wherein a circular support plate for placing the culture dishes is arranged in the culture cylinder, pull rods are symmetrically arranged at two ends of the support plate, an opening is formed in the upper end of the culture cylinder, a sealing cover is sleeved on the opening, a ventilation opening is reserved in the middle of the sealing cover, and a first breathable film covers the ventilation opening; the culture dish comprises a self-contained dish cover, a strip-shaped ventilation groove is formed in the side surface of the culture dish, and a second ventilation film covers the strip-shaped ventilation groove; the top surface of the dish cover is provided with a raised positioning ring, the bottom surface of the culture dish is provided with a raised positioning ring, and the positioning ring can be sleeved outside the positioning ring.

Preferably, the culture cylinder and the culture dish are both transparent structures.

Preferably, the pull rod is vertically fixed on the supporting plate, the upper end of the pull rod is provided with a spherical end, and the length of the pull rod is smaller than the depth of the culture cylinder.

Preferably, the diameter of the supporting plate is larger than that of the culture dish, a raised limiting ring is arranged on the supporting plate, and the size of the limiting ring is equal to that of the positioning ring.

Preferably, the first breathable membrane and the second breathable membrane both adopt PTFE tissue culture membranes.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model arranges a culture dish with a multilayer stack structure in the culture cylinder, which is convenient for multi-group stem cell culture and improves the space utilization rate of the culture cylinder; the positioning lantern ring on the bottom surface of the culture dish is sleeved on the positioning ring on the dish cover, so that the upper and lower layers of culture dishes are aligned and limited conveniently, are not easy to slide, and the stability of stacking is ensured; meanwhile, a supporting plate is arranged in the culture cylinder, and a pull rod is arranged on the supporting plate and used for integrally feeding the stacked culture dishes into the culture cylinder or integrally taking the stacked culture dishes out of the culture cylinder, so that the operation is convenient.

2. Set up ventilative mouthful on the sealed lid of a culture section of thick bamboo to cover first ventilated membrane, set up the ventilative groove of bar at each culture dish lateral wall, and cover the second ventilated membrane, first ventilated membrane and second ventilated membrane are PTFE group and organize the membrane, PTFE group banks up the membrane and can effectively balance the inside and outside atmospheric pressure of culture apparatus, supplements the required gas of culture apparatus inner cell simultaneously, under the condition of guaranteeing the air exchange, can avoid bacterium and virus to get into in the culture apparatus simultaneously, reach the purpose that prevents the pollution.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a schematic view of a specific structure of the support plate of the present invention;

FIG. 3 is a schematic view of the culture dish and its own dish cover;

FIG. 4 is a schematic view of the bottom structure of the culture dish of the present invention;

fig. 5 is a schematic view of a stacking structure of a plurality of culture dishes according to the present invention.

In the figure: 1. a culture cylinder; 2. a support plate; 3. a pull rod; 4. a culture dish; 5. a sealing cover; 6. a ventilation opening; 7. a first breathable film; 8. a dish cover; 9. a strip-shaped ventilation groove; 10. a second breathable film; 11. a positioning ring; 12. a positioning collar; 13. a limit ring.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Referring to fig. 1-5, the present invention provides a technical solution: a device for culturing primary stem cells by a tissue block method comprises a culture cylinder 1 and a culture dish 4 stacked in the culture cylinder 1, wherein a circular support plate 2 for placing the culture dish 4 is arranged in the culture cylinder 1, pull rods 3 are symmetrically arranged at two ends of the support plate 2, the upper end of the culture cylinder 1 is opened and sleeved with a sealing cover 5, a ventilation opening 6 is reserved in the middle of the sealing cover 5, and a first breathable film 7 covers the ventilation opening 6; the culture dish 4 comprises a dish cover 8, a strip-shaped air-permeable groove 9 is formed in the side surface of the culture dish 4, and a second air-permeable film 10 covers the strip-shaped air-permeable groove 9; the top surface of the dish cover 8 is provided with a raised positioning ring 11, the bottom surface of the culture dish 4 is provided with a raised positioning ring 12, and the positioning ring 12 can be sleeved outside the positioning ring 11.

Further, the culture cylinder 1 and the culture dish 4 are both transparent structures.

Furthermore, the pull rod 3 is vertically fixed on the supporting plate 2, a spherical end is arranged at the upper end of the pull rod 3, and the length of the pull rod 3 is smaller than the depth of the culture cylinder 1.

Furthermore, the diameter of the support plate 2 is larger than that of the culture dish 4, a raised limit ring 13 is arranged on the support plate 2, and the size of the limit ring 13 is equal to that of the positioning ring 11.

Further, the first breathable film 7 and the second breathable film 10 both adopt PTFE tissue culture films.

The working principle is as follows: after the culture is injected into each culture dish 4 in the sterile environment, the dish cover 8 is covered, and the corresponding label is attached; the culture dishes 4 are sequentially stacked up and down in sequence, so that the positioning lantern ring 12 on the bottom surface of the upper layer of culture dish 4 is sleeved on the positioning ring 11 on the dish cover 8, the upper layer of culture dish 4 and the lower layer of culture dish 4 are convenient to align and limit, the culture dishes are not easy to slide off, and the stacking stability is ensured;

then placing the stacked culture dishes 4 on the support plate 2, so that the positioning lantern ring 12 on the bottom surface of the culture dish 4 at the lowest layer is sleeved on the limiting ring 13 on the support plate 2, and the stable connection of the whole culture dish is ensured; the whole stacked culture dish 4 is sent into the culture cylinder 1 along with the support plate 2 through the pull rod 3, and the seal cover 5 is covered; the cartridge 1 is then transferred to a suitable environment for culturing.

First ventilated membrane 7 on the sealed lid 5 forms double-deck protective structure with second ventilated membrane 10 on the ventilative groove of bar of each culture dish 4 lateral walls, and first ventilated membrane 7 and second ventilated membrane 10 are PTFE group and cultivate the membrane, can effectively balance the inside and outside atmospheric pressure of culture apparatus, replenish the required gas of culture apparatus inner cell simultaneously, under the condition of guaranteeing the air exchange, can avoid bacterium and virus to get into culture apparatus simultaneously in, reach the purpose that prevents pollution.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A device for culturing primary stem cells by using a tissue block method comprises a culture cylinder (1) and a culture dish (4) stacked in the culture cylinder (1), and is characterized in that a circular support plate (2) for placing the culture dish (4) is arranged in the culture cylinder (1), pull rods (3) are symmetrically arranged at two ends of the support plate (2), a sealing cover (5) is sleeved at an opening at the upper end of the culture cylinder (1), a ventilation opening (6) is reserved in the middle of the sealing cover (5), and a first ventilation film (7) covers the ventilation opening (6); the culture dish (4) comprises a dish cover (8) arranged on the culture dish, a strip-shaped ventilation groove (9) is formed in the side surface of the culture dish (4), and a second ventilation film (10) covers the strip-shaped ventilation groove (9); the top surface of the dish cover (8) is provided with a raised positioning ring (11), the bottom surface of the culture dish (4) is provided with a raised positioning sleeve ring (12), and the positioning sleeve ring (12) can be sleeved outside the positioning ring (11).

2. The apparatus for culturing primary stem cells by tissue block method according to claim 1, wherein: the culture cylinder (1) and the culture dish (4) are both transparent structures.

3. The apparatus for culturing primary stem cells by tissue block method according to claim 1, wherein: the pull rod (3) is vertically fixed on the supporting plate (2), a spherical end is arranged at the upper end of the pull rod, and the length of the pull rod (3) is smaller than the depth of the culture cylinder (1).

4. The apparatus for culturing primary stem cells by tissue block method according to claim 1, wherein: the diameter of the supporting plate (2) is larger than that of the culture dish (4), a raised limiting ring (13) is arranged on the supporting plate (2), and the size of the limiting ring (13) is equal to that of the positioning ring (11).

5. The apparatus for culturing primary stem cells by tissue block method according to claim 1, wherein: the first breathable film (7) and the second breathable film (10) both adopt PTFE tissue culture films.