CN201195726Y - Combined cell culture bottle - Google Patents

Abstract

The utility model relates to a combined cell culture bottle which is provided with good gas permeable ability and installs cell samples three-dimensionally. The combined cell culture bottle is characterized by comprising five parts of a culture bottle, a sample combined frame, a cell culture piece, semi-permeable membrane and a bottle cap, wherein after the cell culture piece is inserted into the sample combined frame, the sample combined frame with the cell culture piece is transferred into the culture bottle and is added with cell culture solution, the bottle cap is covered, the semi-permeable membrane is pressed between a culture bottleneck and the bottle cap, and a complete gas permeable cell culture system is formed. The combined cell culture bottle which is provided by the utility model satisfies cytology test requirements that the culture solution availability factor is improved, the cell culture environment is optimized, the operation performance is improved, and the like.

Description

The built-up type Tissue Culture Flask

Technical field the utility model relates to a kind of Tissue Culture Flask, particularly a kind of air-permeable Tissue Culture Flask with sample combination ability.

Background technology is present, and the culturing bottle that use in a large number in the laboratory is the standard cell lines bottle.This class Tissue Culture Flask is made up of bottle and lid, and cell is attached at the bottom of the culturing bottle.This class Tissue Culture Flask has following obvious deficiency:

1. the Tissue Culture Flask volume is big, sample size is little, causes that the cell culture fluid consumption is big, utilization ratio is low;

2. cell attaches the growth of bottle baseplane, sampling difficulty when subsequent experimental is analyzed, and the sample effective rate of utilization is low;

3. culturing bottle does not have ventilating structure, and ventilation property is poor, is unfavorable for the cell growth;

Therefore, develop a kind of air-permeable, large sample, easy to operate Tissue Culture Flask is very necessary.

The summary of the invention the purpose of this utility model provides a kind of penetrating ability of good gas that has, cell sample is the special cells culturing bottle of three-dimensional array structure, satisfy to improve the nutrient solution service efficiency, optimizes cell culture environment, improves cytologic experiment requirement such as processing property.

The built-up type Tissue Culture Flask comprises culturing bottle, sample combined bay, cell cultures sheet, semi-permeable membranes and bottle cap five parts composition.After the cell cultures sheet inserted the sample combined bay, the sample combined bay that will have the cell cultures sheet changed in the culturing bottle, added cell culture fluid, covered bottle cap, semi-permeable membranes is pressed on cultivates between bottleneck and the bottle cap, formed a complete cell culture system.

The sample combined bay is band cross member closed-in construction, and crossbeam and framework both sides uniformly-spaced process a plurality of slots, and the cell cultures sheet is fixed on the sample combined bay by slot.

The bottle cap punching, the inside and outside gas of bottle exchanges by semi-permeable membranes.

After the utility model adopts technique scheme, have following distinguishing feature:

1. the cell sample amount is big, is distributed in three dimensions, cell culture fluid utilization ratio height;

2. cell sample is the box-like cultivation of groups of slots, and sample is taken conveniently, strong operability, sample utilization ratio height;

3. Tissue Culture Flask has the penetrating ability of good gas, has effectively ensured in the cell growth process demand to gases such as oxygen, carbonic acid gas.

Description of drawings Fig. 1 shows built-up type Tissue Culture Flask basic structure;

Fig. 2 shows the sample combined frame structure, and wherein 2-a is that sample combined bay front view, 2-b are that sample combined bay left view, 2-c are sample combined bay vertical view.

Embodiment culturing bottle 1, sample combined bay 2 and bottle cap 5 are by the transparent polystyrene injection moulding, and culturing bottle 1 internal diameter is 38mm, dark 38mm, wall thickness 1mm; Bottle cap 5 high 8mm, wall thickness 1mm, culturing bottle 1 and bottle cap 5 are used thread connection, and screw thread is molded directly within 1 mouthful on culturing bottle and bottle cap 5 inboards; Sample combined bay 2 wall thickness 1.5mm, the dark 1mm of fluting on the wall, wide 0.6mm can install 6 cell cultures sheets; Cell cultures sheet 3 is by medical glass machine-shaping, cell cultures face width 22mm, long 26mm, thick 0.5mm; Semi-permeable membranes 4 is by medical grade silicon rubber processing, thick 0.1mm.

Culturing bottle 1, sample combined bay 2, cell cultures sheet 3, semi-permeable membranes 4 and bottle cap 5 are all used 120 ℃ of high-temperature steam sterilization 30min.

Behind the cell attachment of inoculation on the cell cultures sheet 3,6 cell cultures sheets 3 are inserted in the slot in the sample combined bay 2 successively, the sample combined bay 2 that cell cultures sheet 3 is installed is put into culturing bottle 1, add cell culture fluid, the cell culture fluid liquid level should surpass the cell cultures sheet 3mm of the superiors, semi-permeable membranes 4 is installed in 1 mouthful on culturing bottle, compresses with bottle cap 5.

Change in the conventional cell culture incubator after the assembling of built-up type Tissue Culture Flask finishes and cultivate.

After experiment finished, twist-off closure 5 was removed semi-permeable membranes 4, took out sample combined bay 2 backs and just cell cultures sheet 3 can be taken out successively.

Claims (1)

1. A kind of built-up type Tissue Culture Flask comprises culturing bottle, sample combined bay, cell cultures sheet, semi-permeable membranes and bottle cap five parts, and it is characterized in that: the cell cultures sheet is fixed on the sample combined bay in the slot mode.

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