CN201883097U - Low-temperature nitrobacteria culture box and culture device - Google Patents

Abstract

A low-temperature nitrifying bacteria incubator and a culture device relate to a bacteria culture device. It solves the shortcoming of complicated structure of the existing bacteria culture device. The nitrifying bacteria incubator is cylindrical, with an air inlet pipe and a water inlet pipe on the bottom surface, and a drain pipe on the top surface. An aeration chamber and three cultivation chambers are arranged in the cylinder from bottom to top. Aeration stones are installed in the room; water intake pipes and sampling ports are provided on the side walls of each cultivation room, and the opening of each sampling port is sealed with a transparent cover; membrane modules are installed on the top of the third cultivation room, and three The cultivation chambers are all filled with membrane-hanging biospheres. The cultivation device uses an air pump to supply air to the intake pipe, and a water pump to supply water to the water intake pipe. The controller controls the work of the water pump and the air pump according to the oxygen content in the three cultivation chambers, the water flow in the water intake pipe, and the air flow in the intake pipe. The utility model has the advantages of high aeration efficiency and stable operation, and can realize the inoculation of efficient bacterial flora.

Description

Low temperature nitrifying bacteria incubator and cultivation device

technical field

The utility model relates to a bacteria cultivation device.

Background technique

In recent years, my country's aquaculture industry has developed rapidly, and its aquaculture production accounts for 50% of the world's aquaculture production. However, at present, my country's aquaculture mainly adopts the extensive traditional farming mode. During the breeding process, due to the feeding and decomposition of bait and the production of fish and shrimp excrement, the organic matter, ammonia nitrogen, phosphorus and other nutrients in the breeding water seriously exceed the standard, which not only causes eutrophication of the water body, but also affects the aquatic ecological environment. The pollution of the water body has endangered the continued development of the aquaculture industry. With the rapid development of my country's economy, the pressure of environment and water resource consumption has seriously restricted the development of traditional aquaculture production methods.

Intensive industrialized aquaculture is an industrialized production method that applies modern industrial technology, modern biological technology and automatic control technology for aquaculture. It is based on high oxygen and temperature, and operates in a way of water recycling, with high breeding density. , fast growth, low bait coefficient, and few diseases, not only eliminates the interference and influence of environmental and external water quality pollution, but also does not pollute water resources, which is an inevitable trend in the development of aquaculture technology. The ensuing problem urgently to be solved is how to prolong the service life of the culture circulation water. The key to the development of industrial aquaculture technology is the water treatment technology of the circulation system.

Ammonia nitrogen is an important metabolic end product of fish and shrimp, and one of the main pollutions of aquaculture. The high concentration of nutrients in water is especially ammonia nitrogen. Ammonia nitrogen has a serious impact on the catalysis of enzymes and the stability of cell membranes in fish and shrimp, and destroys the excretory system and osmotic balance. Removing excessive ammonia nitrogen in aquaculture water is of great significance to improving the recirculation aquaculture system.

Among the methods for treating ammonia nitrogen, microbial treatment to remove ammonia nitrogen from water is an ideal method. Through the process of nitrification and denitrification, the toxic substance ammonia nitrogen in the wastewater is converted into ammonia gas and released from the water body. Since the organic matter contained in the wastewater itself can be used as a carbon source in the denitrification process, it can reduce or eliminate the need for additional carbon sources, carbon removal and nitrogen removal can be carried out at the same time, and the wastewater can be purified and recycled to achieve the purpose of saving water resources and treatment costs .

However, the existing nitrifying bacteria culture device is complex in structure and expensive, which is the main factor limiting its popularization and use.

Utility model content

The utility model provides a low-temperature nitrifying bacteria incubator and a cultivation device with simple structure.

The nitrifying bacteria incubator described in the utility model is a cylinder, an air inlet pipe and a water inlet pipe are arranged on the bottom surface of the cylinder, and a drain pipe is arranged on the top surface of the cylinder, and the cylinder is sequentially arranged from bottom to top: The aeration chamber, the first cultivation chamber, the second cultivation chamber and the third cultivation chamber are provided with isolation nets between the aeration chamber, the first cultivation chamber, the second cultivation chamber and the third cultivation chamber, and in the aeration chamber Aeration stones are provided; water intake pipes and sampling ports are provided on the side walls of each culture room, and the opening of each sampling port is sealed with a transparent cover, which serves as an observation window; on the top of the third culture room A membrane assembly is provided, and the first culture chamber, the second culture chamber and the third culture chamber are all filled with membrane-hanging biospheres.

The culture device based on the above-mentioned low-temperature nitrifying bacteria incubator is composed of a nitrifying bacteria incubator, a controller, three oxygen sensors, a liquid flow meter, a gas flow meter, a water pump and an air pump. The trachea is connected with the air pump, and three oxygen sensors are respectively arranged in the three cultivation chambers for measuring the oxygen content in the three cultivation chambers, the gas flowmeter is used for measuring the gas flow in the intake pipe, and the liquid flowmeter is used for Collecting the liquid flow in the water inlet pipe, the gas flow information output end of the gas flow meter is connected to the gas flow signal acquisition end of the controller, the liquid flow information output end of the liquid flow meter is connected to the liquid flow information acquisition end of the controller, The signal output ends of the three oxygen sensors are connected to the three oxygen signal acquisition ends of the controller, the air pump control signal output end of the controller is connected to the control signal input end of the air pump, and the water pump control signal output end of the controller is connected to the water pump's Control signal input terminal.

The utility model combines the characteristics of aquaculture to design a nitrifying bacteria culture device with simple structure and complete functions. It has the advantages of high aeration efficiency, stable performance, stable operation, and convenient process operation. It is also designed to facilitate the monitoring of system operating conditions and the inoculation of high-efficiency bacteria, which is convenient for popularization and application, and is suitable for mass cultivation of nitrifying bacteria.

The utility model can improve the depth and breadth of nitrifying bacteria research, and expand the application space of low-temperature nitrifying bacteria in the field of aquaculture in industrial culture technology. To study and utilize the nitrification of low-temperature nitrifying bacteria, develop economical nitrifying bacteria enrichment technology, increase the yield of nitrifying bacteria, accelerate the treatment of aquaculture pollutants, and reduce the environmental pollution load are of great significance to my country's sewage treatment and environmental protection. Significance.

Description of drawings

Fig. 1 is the structural representation of the low-temperature nitrifying bacteria incubator of the present utility model. Fig. 2 is an electrical schematic diagram of the low-temperature nitrifying bacteria cultivation device.

Detailed ways

Specific embodiment one: the low-temperature nitrifying bacteria incubator described in this embodiment is a cylinder, an air inlet pipe 3 and a water inlet pipe 16 are arranged on the bottom surface of the cylinder, and a drain pipe 9 is arranged on the top surface of the cylinder , the cylinder is followed by the aeration chamber 14, the first cultivation chamber 5, the second cultivation chamber 7 and the third cultivation chamber 8 from bottom to top, in the aeration chamber 14, the first cultivation chamber 5, the second cultivation chamber 7 and Isolation nets 4 are arranged between the third cultivation chambers 8, and aeration stones 15 are arranged in the aeration chambers 14; water intake pipes 6 and sampling ports 12 are all arranged on the side walls of each cultivation chamber, and each sampling The opening of the mouth 12 is sealed with a transparent cover, which is used as an observation window 13; a membrane assembly is arranged on the top of the third cultivation chamber 8, and in the first cultivation chamber 5, the second cultivation chamber 7 and the third cultivation chamber 8 Both are filled with bio-balls.

The membrane module in this embodiment is a membrane module with micropores, which is used to prevent nitrifying bacteria from flowing out from the water outlet.

The pore diameter of the membrane module in this embodiment is between 0.1 μm and 0.2 μm. It can be made of polypropylene (PP) membrane, hollow fiber and microporous membrane.

In this embodiment, water intakes 6 are provided on the side walls of the three cultivation chambers, which is convenient for technicians to collect water samples in each cultivation chamber for laboratory testing at any time.

In this embodiment, sampling ports are provided on the side walls of the three cultivation chambers, which is convenient for technicians to obtain bacterial samples in any one of the cultivation chambers through the sampling ports at any time for detection and analysis. In this embodiment, each sampling port is sealed with a transparent cover, which is an observation window, so that technicians can observe the conditions in each culture room at any time during the bacterial culture process, and know the conditions in each culture room at any time. Condition.

In this embodiment, the sampling port on the side wall of each culture chamber is located at the center of the height of the culture chamber.

In this embodiment, the diameters of the membrane-hanging biospheres 5 in the three cultivation chambers may be different, and the diameters of the membrane-hanging biospheres increase sequentially from bottom to top.

The bottom of the nitrifying bacteria incubator of the present embodiment is an aeration chamber, and oxygenation is added to water by an air pump and an aeration stone. consumption, so that the oxygen content in the second cultivation chamber 7 is reduced, and the oxygen content in the third cultivation chamber 8 is the least. Since the oxygen content in each cultivation chamber is different, it can be adapted to the cultivation of different bacteria.

Specific embodiment two: this embodiment is a further limitation to the nitrifying bacteria incubator described in specific embodiment one, in the nitrifying bacteria incubator described in this embodiment, the diameter of the membrane-hanging biosphere in the first cultivation chamber 5 The diameter of the membrane-hanging biospheres in the second cultivation chamber 7 is 2.2-2.8mm, and the diameter of the membrane-hanging biospheres in the third cultivation chamber 8 is 3.0mm.

Specific embodiment three: What this embodiment described is the nitrifying bacteria cultivation device based on the nitrifying bacteria incubator described in specific embodiment one or two, the device consists of a nitrifying bacteria incubator, a controller 19, three oxygen sensors 11, The liquid flow meter 17, the gas flow meter 2, the water pump 18 and the air pump 1 are composed, the water inlet pipe 16 of the nitrifying bacteria incubator is connected with the water pump 18, the air inlet pipe 3 is connected with the air pump 1, and the three oxygen sensors 11 are respectively arranged in three In the cultivation room, it is used to measure the oxygen content in the three cultivation chambers. The gas flowmeter 2 is used to measure the gas flow in the inlet pipe 3, and the liquid flowmeter 17 is used to collect the liquid flow in the water inlet pipe 16. The gas flow information output end of the flow meter 2 is connected to the gas flow signal acquisition end of the controller 19, the liquid flow information output end of the liquid flow meter 17 is connected to the liquid flow information acquisition end of the controller 19, and the signals of the three oxygen sensors 11 The output end is connected to the three oxygen signal acquisition ends of the controller 19, the air pump control signal output end of the controller 19 is connected to the control signal input end of the air pump 1, and the water pump control signal output end of the controller is connected to the control signal of the water pump 18 input.

In actual work, the controller 19 controls the operation of the air pump 1 and the water pump 18 according to the oxygen content information collected by the oxygen sensors 11 located in the three cultivation chambers, and monitors the aeration chamber in real time through the gas flow meter 2 and the liquid flow meter 17 The amount of air intake and water intake can be controlled, and then the flow rate of gas and water in the entire device can be controlled.

Claims (7)

1. low-temperature nitrifying bacteria incubator, it is characterized in that, described low-temperature nitrifying bacteria incubating box is cylinder type, is provided with inlet pipe (3) and water inlet pipe (16) on the bottom surface of this cylinder, in this cylinder A drainpipe (9) is arranged on the top surface, and the cylinder is followed by an aeration chamber (14), a first cultivation chamber (5), a second cultivation chamber (7) and a third cultivation chamber (8) from bottom to top, Between the aeration chamber (14), the first cultivation chamber (5), the second cultivation chamber (7) and the third cultivation chamber (8), an isolation net (4) is arranged, and in the aeration chamber (14) Aeration stones (15) are provided; water intake pipes (6) and sampling ports (12) are arranged on the side walls of each cultivation chamber, and the opening of each sampling port (12) is sealed with a transparent cover. The cover is used as an observation window (13); a membrane module (10) is arranged on the top of the third cultivation chamber (8), and the first cultivation chamber (5), the second cultivation chamber (7) and the third cultivation chamber (8) Both are filled with bio-balls. 2. The nitrifying bacteria incubator according to claim 1, characterized in that, the micropore diameter of the membrane module (10) is between 0.1 μm-0.2 μm. 3. The nitrifying bacteria incubator according to claim 1, characterized in that, said membrane assembly (10) is formed by stacking polypropylene membranes, hollow fibers and microporous membranes. 4. The nitrifying bacteria incubator according to claim 1, wherein the sampling port on the side wall of each cultivation chamber is located at the center of the height of the cultivation chamber. 5. The nitrifying bacteria incubator according to claim 1, characterized in that, the diameters of the membrane-hanging biospheres in the three cultivation chambers are different, and from bottom to top, the diameters of the membrane-hanging biospheres increase successively. 6. nitrifying bacteria incubator according to claim 1, is characterized in that, the diameter of the membrane-hanging biological ball in the first cultivation chamber (5) is 2mm, the diameter of the membrane-hanging biological ball in the second cultivation chamber (7) The diameter is 2.5mm, and the diameter of the membrane-hanging biosphere in the third cultivation chamber (8) is 3.0mm. 7. based on the cultivating device of the described low-temperature nitrifying bacteria incubator according to any one of claims 1 to 6, it is characterized in that it also includes controller (19), three oxygen sensors (11), liquid flow meter (17 ), gas flowmeter (2), water pump (18) and air pump (1), the water inlet pipe (16) of the nitrifying bacteria incubator is connected with the water pump (18), and the air inlet pipe (3) is connected with the air pump (1), Three oxygen sensors (11) are respectively arranged in the three cultivation chambers, and are respectively used to measure the oxygen content in the three cultivation chambers, and the gas flow meter (2) is used to measure the gas flow in the intake pipe (3), and the liquid The flow meter (17) is used to collect the liquid flow in the water inlet pipe (16), the gas flow information output end of the gas flow meter (2) is connected to the gas flow signal acquisition end of the controller (19), and the liquid flow meter The liquid flow information output terminal of (17) is connected with the liquid flow information collection terminal of the controller (19), and the signal output terminals of the three oxygen sensors (11) are connected with the three oxygen signal collection terminals of the controller (19). The air pump control signal output end of the controller (19) is connected to the control signal input end of the air pump (1), and the water pump control signal output end of the controller is connected to the control signal input end of the water pump (18).

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