CN203346380U - Multifunctional bacterial biofilm generation device - Google Patents
Multifunctional bacterial biofilm generation device Download PDFInfo
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- CN203346380U CN203346380U CN 201320377746 CN201320377746U CN203346380U CN 203346380 U CN203346380 U CN 203346380U CN 201320377746 CN201320377746 CN 201320377746 CN 201320377746 U CN201320377746 U CN 201320377746U CN 203346380 U CN203346380 U CN 203346380U
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Abstract
The utility model discloses a multifunctional bacterial biofilm generation device which comprises a culture box and a breathable culture box cover, wherein an inner culture box matched with the inner space of the culture box is also arranged in the culture box; multiple pairs of crossed slots arranged along the same direction are formed on the two opposite sides of the inner culture box; each pair of crossed slots is composed of a vertical slot vertical to the bottom surface of the inner culture box and a non-vertical inclined slot inclining relative to the bottom of the inner culture box; a first clamp strip and a first clamp slot are formed at the front and back ends of the inner culture box respectively; a second clamp slot and a second clamp strip matched with the first clamp strip and the first clamp slot are arranged at the front and back ends of the interior of the culture box respectively; multiple horizontal slots in up-down arrangement which extend from the first clamp slot to the first clamp strip and into which a biofilm attachment piece can be inserted are formed on the two sides of the inner culture box; multiple holes are formed on the side of the inner culture box; a third clamp strip is arranged at the front end of the culture box; a third clamp slot matched with the third clamp strip is formed at the back end of the culture box; a notch is formed at the lower end of the biofilm attachment piece. The devices disclosed by the utility model can be used independently or connected end to end to meet the needs of different experimental purposes and the needs for the generation and growth of different types of bacterial biofilms; moreover, the designed structure is simple, the manufacturing cost is low, the materials are easily available, and the device is convenient for industrial batch production and large-area popularization to a laboratory.
Description
Technical field
The utility model belongs to bacterial biof iotalm and studies small-sized supplementary unit field, is specifically related to a kind of multi-functional bacterial biof iotalm generating unit.
Background technology:
Inside and outside physical environment, industrial system and animal and plant body, bacterium is not that the mode with floating state exists, and approximately has 90% bacterium to exist with biomembranous form, or at least sticks to body surface.The microbial film that the spontaneous gathering of cell forms is a special colony, wherein except cell itself, also comprise a large amount of extracellular matrixs (Extracellular Polymeric Substances of emiocytosis, EPS), due to the diversity of composition, the biofilm structure of formation is also varied.Biomembranous forming process mainly comprises following three steps: be at first thalline sticking on the upholder surface; Then be biomembranous development, i.e. the formation of microcolony; Be finally biomembranous maturation, microbial film now possesses the ability of stronger opposing external pressure, as resistance etc.Monitor and grasp biomembranous generation and evolution and will be conducive to better control and remove microbial film.
And the artificial membrane method for generation mainly contains test tube method at present, Microdilution plate method, put the sheet method, dull and stereotyped Diaphragm Method, the pipeline method, flow the chamber method and revolve dish method etc., every kind of method has the features and applications of oneself, but up to the present, still do not have special equipment or device to provide for biomembranous formation the carrier that occurs and develop, in the time of more, it is the situation that test according to oneself in each laboratory, and utilize the inner existing condition in laboratory and facility to carry out biomembranous research, this research has the characteristics of unicity and specificity, be unfavorable for the comparison of experimental result, because facility and condition that different laboratories is used are different often.Urgently need a kind of microbial film generating unit of standard type simple type multifunctional type to occur.
And use at present more artificial membrane generating unit as Microdilution plate method and put sheet method etc., all there are some deficiency and defects more or less.As the method mid-of microwell plate, owing to there is no on the market at present just in time can be placed on sheet glass in porous plate (comprising 96 holes, 48 holes, 24 holes) and stainless steel substrates etc., so these microbial film attachment material often need experimenter oneself to make, and the small pieces of these making, may there is more individual difference, on the result of testing, can produce important impact, and the porous plate used, be not many times to need an entire plate, just need several or tens holes, and the porous plate of most is all the monoblock sterile packed, single hole or single-row or single file hole all can not freely be dismantled, thereby cause in the situation that only need the minority hole, cause the waste of porous plate, sometimes, biomembranous research need to be observed microbial film at the dynamic changing process adhered on carrier, need at set intervals the carrier adhered to is taken out to processing, and due to the globality of porous plate, can cause the pollution of thalline in the open plate lid, just be put into the carrier adhered in test tube or plastic centrifuge tube at last or in other container, also exist the inconvenience problem of placing and operating.
Simultaneously, some bacteriums are facultative anaerobes, and its microbial film mainly forms at gas-liquid interface, and some bacteriums are strictly anaerobic bacteriums, its microbial film mainly forms in the bottom of substratum, so need a kind of generation thing film device that can meet easily above two kinds of requirements of design.The design of generation thing film device in the past is only to consider the biomembranous formation of gas-liquid interface, do not consider that some bacteriums are anerobes, its biomembranous formation is more the bottom that appears at substratum, the more important thing is, along with the prolongation of incubation time, the bottom of container can be assembled and deposit to thalline.So need a kind of rational multi-functional culture apparatus of design to meet dissimilar bacterial biof iotalm growth needs.
Summary of the invention:
The purpose of this utility model is to provide a kind of can be separately practical, also can join end to end, and to meet the coherence request of experiment condition, and can meet the multi-functional bacterial biof iotalm generating unit of generation and the growth of dissimilar bacterial biof iotalm.
The utility model is implemented by the following technical programs:
Multi-functional bacterial biof iotalm generating unit of the present utility model, it is characterized in that, comprise and cultivate box and ventilative cultivation lid, also be provided with and the interior cultivation box of cultivating the box internal space and adapting cultivating box inside, the relative both sides of described interior cultivation box are provided with some intersection slots to arranging in the same direction, each intersects slot by the vertical slot perpendicular to interior cultivation box bottom surface and non-perpendicular, be skewed oblique cutting groove with interior cultivation box bottom surface and intersect formation, every pair of vertical slot and oblique cutting groove intersected between slot is mutually corresponding, every pair of vertical slot or oblique cutting groove intersected in slot can insert the microbial film attachment flaps, be respectively equipped with the first card article and the first draw-in groove in the rear and front end of described interior cultivation box, rear and front end in cultivating box is respectively equipped with the second draw-in groove and the second card article adapted with the first card article and the first draw-in groove, make the first card article can snap in the second draw-in groove, the second card article can snap in (being conducive to the insertion that interior cultivation box is more firm cultivates in box) in the first draw-in groove, have in interior cultivation box both sides and extended to the first card article by the first draw-in groove, can insert several horizontal slot that are arranged above and below of microbial film attachment flaps, there are many holes (be convenient to substratum and cultivating flowing and exchange between box and interior cultivation box) on the side of interior cultivation box, front end at described cultivation box is provided with the 3rd card article, be provided with in the rear end of cultivating box the 3rd draw-in groove adapted with the 3rd card article, the bottom of described microbial film attachment flaps has breach, make the interior cultivation box internal space separated by the microbial film attachment flaps to communicate and (be conducive to the exchange of substance between microbial film and liquid, be conducive to biomembranous growth and maturation).The 3rd card article can match and block with rear end the 3rd draw-in groove of the multi-functional bacterial biof iotalm generating unit of another one, the 3rd draw-in groove also can match and block with front end the 3rd card article of the multi-functional bacterial biof iotalm generating unit of another one, thereby can make to be connected before and after a plurality of multi-functional bacterial biof iotalm generating units, meet different experiment demands.
Preferably, in the middle of described microbial film attachment flaps top, have groove, this groove is convenient to the microbial film attachment flaps is extracted from slot.
Preferably, described cultivation lid lower surface has and cultivates rectangular that box internal space shape adapts, and is convenient to cultivate on the lid of lid and stable, makes the tightr of lid, prevents living contaminants.Further preferably in the left and right edges position of the lower surface of cultivating lid, have two rectangular, two rectangular just in time with cultivate the space on top after box and the combination of interior cultivation box and match and cover, be conducive to more tight by the upper cap of whole device, prevent living contaminants.
Described cultivation lid has airing function, and its material is silica gel.
The whole multi-functional bacterial biof iotalm generating unit combined both can be placed on standing cultivation in incubator, also can put into constant-temperature table with certain rotating speed rotating and culturing.
The utility model is to use like this, the microbial film attachment flaps is inserted in vertical slot or oblique cutting groove or horizontal slot, interior cultivation box transfer access substratum and bacterium, then cover the cultivation box with the cultivation lid, words if necessary, can utilize the 3rd card article and the 3rd draw-in groove and another one or a plurality of multi-functional bacterial biof iotalm generating unit to be joined end to end, then this linker being put into to incubator is cultivated, after for some time, can be by the multi-functional bacterial biof iotalm at two ends wherein, perhaps all multi-functional bacterial biof iotalm are taken off, open the cultivation lid, gently interior cultivation box is taken out, then the microbial film attachment flaps can be taken out from interior cultivation box carry out such as dyeing etc. post-processed, also interior cultivation box can be comprised to the combination that wherein is inserted with the microbial film attachment flaps puts into a new cultivation box again, again add fresh substratum and bacterium liquid, carry out second incubation.
The utility model can independently be used, also can join end to end, to meet different experiment purpose needs, and can meet generation and the growth of dissimilar bacterial biof iotalm, its project organization is simple, cost of manufacture is cheap, and material is easy to get, and is convenient to industrial mass manufacture and laboratory spread.
The accompanying drawing explanation:
Fig. 1 is the structural representation of multi-functional bacterial biof iotalm generating unit of the present utility model;
Fig. 2 is the structural representation of cultivating box;
Fig. 3 is the structural representation of interior cultivation box;
Fig. 4 is the structural representation of microbial film attachment flaps;
Fig. 5 is the structural representation of cultivating lid;
Wherein 1, cultivate box; 2, interior cultivation box; 3, microbial film attachment flaps; 4, cultivate lid; 5, vertical slot; 6, oblique cutting groove; 7, horizontal slot; 8, the 3rd card article; 9, the second draw-in groove; 10, the second card article; 11, the first draw-in groove; 12, the first card article; 13, hole; 14, groove; 15, breach; 16, rectangular; 17, the 3rd draw-in groove.
Embodiment:
Following examples are to further illustrate of the present utility model, rather than to restriction of the present utility model.
Embodiment 1:
As shown in Figure 1, the multi-functional bacterial biof iotalm generating unit of the present embodiment, the cultivation box 1 and the rectangular ventilative cultivation lid 4 that comprise cuboid, also be provided with and the interior cultivation box 2 of cultivating the cuboid that the box internal space adapts cultivating box inside, the relative both sides of described interior cultivation box 2 are provided with 3 pairs of tandem intersection slots, each intersects slot by the vertical slot 5 perpendicular to interior cultivation box 2 bottom surfaces and non-perpendicular, be skewed oblique cutting groove 6 with interior cultivation box bottom surface and intersect formation, every pair of vertical slot 5 and oblique cutting groove 6 intersected between slot is mutually corresponding, every pair of vertical slot or oblique cutting groove intersected in slot can insert microbial film attachment flaps 3, be respectively equipped with the first card article 12 and the first draw-in groove 11 in the rear and front end of described interior cultivation box, rear and front end in cultivating box is respectively equipped with the second draw-in groove 9 and the second card article 10 adapted with the first card article 12 and the first draw-in groove 11, make the first card article 12 can snap in the second draw-in groove 9, the second card article 10 can snap in (being conducive to the insertion that interior cultivation box is more firm cultivates in box) in the first draw-in groove 11, there are 3 horizontal slot that are arranged above and below 7 can inserting microbial film attachment flaps 3 of being extended to the first card article 12 by the first draw-in groove 11 in interior cultivation box 2 both sides, there are many hole 13(and be convenient to substratum in the mobile and exchange of cultivating between box and interior cultivation box on the side of interior cultivation box 4), front end at described cultivation box 1 is provided with the 3rd card article 8, be provided with in the rear end of cultivating box 1 the 3rd draw-in groove 17 adapted with the 3rd card article 8, the bottom of described microbial film attachment flaps 3 has breach 15, make the interior cultivation box internal space separated by microbial film attachment flaps 3 to communicate and (be conducive to the exchange of substance between microbial film and liquid, be conducive to biomembranous growth and maturation), there is groove 14 in the middle of top, this groove 14 is convenient to microbial film attachment flaps 3 is extracted from slot.The 3rd card article 8 can match and block with rear end the 3rd draw-in groove 17 of the multi-functional bacterial biof iotalm generating unit of another one, the 3rd draw-in groove 17 also can match and block with front end the 3rd card article 8 of the multi-functional bacterial biof iotalm generating unit of another one, thereby can make to be connected before and after a plurality of multi-functional bacterial biof iotalm generating units, meet different experiment demands.The left and right edges position of the lower surface of cultivating lid 4 have two rectangular 16, two rectangular 16 just in time with cultivate box and the combination of interior cultivation box after the space on top match and cover, be conducive to more tight by the upper cap of whole device, prevent living contaminants.
Claims (3)
1. a multi-functional bacterial biof iotalm generating unit, it is characterized in that, comprise and cultivate box and ventilative cultivation lid, also be provided with and the interior cultivation box of cultivating the box internal space and adapting cultivating box inside, the relative both sides of described interior cultivation box are provided with some intersection slots to arranging in the same direction, each intersects slot by the vertical slot perpendicular to interior cultivation box bottom surface and non-perpendicular, be skewed oblique cutting groove with interior cultivation box bottom surface and intersect formation, every pair of vertical slot and oblique cutting groove intersected between slot is mutually corresponding, every pair of vertical slot or oblique cutting groove intersected in slot can insert the microbial film attachment flaps, be respectively equipped with the first card article and the first draw-in groove in the rear and front end of described interior cultivation box, rear and front end in cultivating box is respectively equipped with the second draw-in groove and the second card article adapted with the first card article and the first draw-in groove, make the first card article can snap in the second draw-in groove, the second card article can snap in the first draw-in groove, have in interior cultivation box both sides and extended to the first card article by the first draw-in groove, can insert several horizontal slot that are arranged above and below of microbial film attachment flaps, there are many holes on the side of interior cultivation box, front end at described cultivation box is provided with the 3rd card article, be provided with in the rear end of cultivating box the 3rd draw-in groove adapted with the 3rd card article, the bottom of described microbial film attachment flaps has breach, make the interior cultivation box internal space separated by the microbial film attachment flaps to communicate.
2. according to the multi-functional bacterial biof iotalm generating unit of claim 1, it is characterized in that thering is groove in the middle of described microbial film attachment flaps top.
3. according to the multi-functional bacterial biof iotalm generating unit of claim 1, it is characterized in that, described cultivation lid lower surface has and cultivates rectangular that box internal space shape adapts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320377746 CN203346380U (en) | 2013-06-27 | 2013-06-27 | Multifunctional bacterial biofilm generation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320377746 CN203346380U (en) | 2013-06-27 | 2013-06-27 | Multifunctional bacterial biofilm generation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203346380U true CN203346380U (en) | 2013-12-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320377746 Expired - Lifetime CN203346380U (en) | 2013-06-27 | 2013-06-27 | Multifunctional bacterial biofilm generation device |
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| Country | Link |
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| CN (1) | CN203346380U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109762718A (en) * | 2019-03-18 | 2019-05-17 | 金婧菲 | A kind of slime mould culture box and application method |
| CN111412860A (en) * | 2020-03-25 | 2020-07-14 | 广东省微生物研究所（广东省微生物分析检测中心） | Observation objective table for laser confocal microscope biofilm |
-
2013
- 2013-06-27 CN CN 201320377746 patent/CN203346380U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109762718A (en) * | 2019-03-18 | 2019-05-17 | 金婧菲 | A kind of slime mould culture box and application method |
| CN111412860A (en) * | 2020-03-25 | 2020-07-14 | 广东省微生物研究所（广东省微生物分析检测中心） | Observation objective table for laser confocal microscope biofilm |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131218 |