CN103773683B - Layer-stepping Tissue Culture Plate - Google Patents
Layer-stepping Tissue Culture Plate Download PDFInfo
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- CN103773683B CN103773683B CN201210410253.7A CN201210410253A CN103773683B CN 103773683 B CN103773683 B CN 103773683B CN 201210410253 A CN201210410253 A CN 201210410253A CN 103773683 B CN103773683 B CN 103773683B
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- 239000003351 stiffener Substances 0.000 claims abstract description 25
- 238000001914 filtration Methods 0.000 claims abstract description 20
- 239000004809 Teflon Substances 0.000 claims abstract description 16
- 229920006362 Teflon® Polymers 0.000 claims abstract description 16
- 239000011521 glass Substances 0.000 claims abstract description 15
- 210000002421 cell wall Anatomy 0.000 claims abstract description 11
- 239000000758 substrate Substances 0.000 claims abstract description 11
- 210000004027 cell Anatomy 0.000 claims description 23
- 230000010261 cell growth Effects 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 230000009286 beneficial effect Effects 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 230000002411 adverse Effects 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 230000003822 cell turnover Effects 0.000 claims description 3
- 210000004748 cultured cell Anatomy 0.000 claims description 3
- 239000007789 gas Substances 0.000 claims description 3
- 230000009545 invasion Effects 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 3
- 241001515965 unidentified phage Species 0.000 claims description 3
- 238000004113 cell culture Methods 0.000 description 7
- 239000012930 cell culture fluid Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- Wood Science & Technology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
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- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
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Abstract
Layer-stepping Tissue Culture Plate, belong to technical field of medical instruments, layer-stepping Tissue Culture Plate by culture plate, cultivate plate hole, cultivate board slot, culture plate cell wall, millipore filtration, plate hole column, breezing plate axis hole, cultivate hole, breezing plate, culture plate hole wall, culture plate vestibule, pillar stiffener, cultivation hole wall, Teflon layer, cultivation hole opening, cultivate at the bottom of hole, cultivate hole substrate and form, it is characterized in that culture plate is made up of glass, top inwardly has one to cultivate board slot, in cultivation board slot, 6 are had to cultivate plate hole; Cultivate plate hole upper part and exceed culture plate trench bottom, cultivating plate hole central authorities has a plate hole column, after plate hole column is through breezing plate axis hole and pillar stiffener, breezing plate can be superimposed from level to level; Pillar stiffener breezing plate is spaced from each other the glass cylinder of 2 millimeters; The upper surface of breezing plate regularly arranged multiple cultivation hole.Layer-stepping Tissue Culture Plate makes simple, workable, with low cost, successful.
Description
Technical field
The present invention relates to a kind of layer-stepping Tissue Culture Plate, belong to medical treatment and laboratory equipment technical field.
Background technology
At present, in cytological research process, often need to cultivate cell, to meet the specific requirement of various experiment, but, in cell cultivation process, because need sterilization, conventional method uses various disposable consumptive material to carry out the experiment of cytobiology, the price of often kind of consumptive material is all higher, and such as one piece of six orifice plate just needs 7 yuans, once tests, just need polylith six orifice plate, thus the expense required for experiment is increased exponentially.In cell cultivation process, namely whether cell cultivates successfully one of mark is whether cell is adherent, whether fissiparity after adherent.Like this, the Area of bearing required for larger Growth of Cells is just needed in order to obtain result preferably.How to reduce the cost of cell cultures, a Tissue Culture Plate is turned out more cell and becomes a great problem being badly in need of solving? so utilize the design of layering and arrange the cultivation hole increasing cell attachment area on each layer, it is necessary for inventing a kind of layer-stepping Tissue Culture Plate cultivating plate hole and have multilayer breezing plate.
Summary of the invention
In order to overcome the less difficult problem of the higher culture area of known Tissue Culture Plate cost, the invention provides a kind of layer-stepping Tissue Culture Plate, this layer-stepping Tissue Culture Plate utilizes breezing plate cultivation plate hole to be divided into multilayer and cultivates face, each cultivation face is designed again multiple cultivation hole, the growth needs supporting plane of cell, because layering adds the design in multiple cultivation hole, expand the area of Growth of Cells, the more known culture plate of the culture area of layer-stepping Tissue Culture Plate is significantly improved, the use of glass and Teflon, culture plate can also be made to be reused, the rear waste namely abandoned resource of known batch cultur plate can be reduced, thus reach the object reducing cell cultures expense.
The technical solution adopted for the present invention to solve the technical problems is:
Layer-stepping Tissue Culture Plate by culture plate 1, cultivate plate hole 2, cultivate board slot 3, culture plate cell wall 4, millipore filtration 5, plate hole column 6, breezing plate axis hole 7, cultivate hole 8, breezing plate 9, culture plate hole wall 10, culture plate vestibule 11, pillar stiffener 12, cultivate hole wall 13, Teflon layer 14, cultivate hole opening 15, to cultivate at the bottom of hole 16, cultivate hole substrate 17 and form, it is characterized in that culture plate 1 is made up of glass, top inwardly has a degree of depth to be the cultivation board slot 3 of 5-10 millimeter, in cultivation board slot 3,6 are had to cultivate plate hole 2; Cultivate plate hole 2 upper part to exceed and cultivate bottom board slot 3, so as millipore filtration 5 can wrap cultivate plate hole 2 opening outside the opening cultivating plate hole 2 is wound around after seal; Cultivate plate hole 2 central authorities and have a plate hole column 6, after plate hole column 6 is through breezing plate axis hole 7 and pillar stiffener 12, breezing plate 9 can be superimposed from level to level; Pillar stiffener 12 breezing plate 9 is spaced from each other the glass cylinder of 2 millimeters; At the upper surface of breezing plate 9, between breezing plate axis hole 7 periphery to breezing plate 9 periphery, regularly arranged multiple diameter is 2 millimeters, the degree of depth is the cultivation hole 8 of 2 millimeters, cultivates the main place that hole 8 is Growth of Cells.
Preferred version of the present invention is: in cultivation board slot 3, have 6 diameters to be 3-3.5 centimetre, the degree of depth is the cultivation plate hole 2 of 1.0-1.8 centimetre; Cultivate plate hole 2 upper part and exceed 4-9 millimeter bottom cultivation board slot 3, so that millipore filtration 5 can wrap the opening cultivating plate hole 2, allow the cell cultivated in plate hole 2 both can avoid the pollution of bacterium, freely can obtain oxygen from the outside again, be beneficial to the breathing of cell, prevent anoxic from bringing adverse influence to cultured cells.
Preferred version of the present invention is: the outwardly convex of cultivating around board slot 3 is culture plate cell wall 4, culture plate cell wall 4 upper surface and the opening cultivating plate hole 2 superscribe the surface after millipore filtration 5 in same level, be convenient to packed and transported, the performance attractive in appearance of culture plate during culturing cell, can also be ensured.
Preferred version of the present invention is: millipore filtration 5 commercially available can ensure that moisture and gas pass through, but the invasion and attack of bacteriophage can be stoped, on the Bechtop disinfected, seal after the opening cultivating plate hole 2 being wound around with millipore filtration 5, cultivate under being then placed on optimal temperature.
Preferred version of the present invention is: have a diameter to be vitreous plate hole column 6 of 2 millimeters cultivating plate hole 2 central authorities, after plate hole column 6 is through breezing plate axis hole 7 and pillar stiffener 12, breezing plate 9 can be superimposed from level to level, fill the culture plate vestibule 11 surrounded by culture plate hole wall 10.
Preferred version of the present invention is: the glass cylinder that adjacent two pieces of breezing plates 9 are spaced from each other 2 millimeters by pillar stiffener 12, diameter is 0.5 centimetre, length is 2 millimeters, and barrel bore is 2.2 millimeters, and cylindrical wall thickness is 1.4 millimeters; Pillar stiffener 12 is positioned at breezing plate 9 central authorities below, and connects as one with breezing plate 9, and the cylinder bore of pillar stiffener 12 is connected up and down with the vestibule of breezing plate axis hole 7, and internal diameter size is 2.2 millimeters.
Preferred version of the present invention is: the plectane that breezing plate 9 is vitreous diameters is 2.5-3 centimetre, thickness is 2.5-3 millimeter, plectane edge and the distance of cultivating between plate hole 2 wall are 2 millimeters, so that band needs the nutrient solution of culturing cell and injects and cultivate in plate hole 2, the diameter of plectane central authorities is the hole of 2.2 millimeters is breezing plate axis hole 7.
Preferred version of the present invention is: the hole that to cultivate hole 8 are regularly arranged between the upper surface, breezing plate axis hole 7 periphery to breezing plate 9 periphery of breezing plate 9, multiple diameters is 2 millimeters, the degree of depth is 2 millimeters, and cultivation hole 8 is main place of Growth of Cells; Each cultivation hole 8 includes and cultivates hole wall 13, Teflon layer 14, cultivate hole opening 15, to cultivate at the bottom of hole 16, cultivate hole substrate 17; Cultivate hole wall 13 and to cultivate at the bottom of hole 16 and jointly surround the inner chamber cultivating hole 8, glass quality, Teflon can be added to breezing plate 9 upper surface according to known loading method and cultivate hole wall 13 and cultivate the surface of at the bottom of hole 16, form the Teflon layer 14 that thickness is 0.1-0.2 millimeter, be conducive to Growth of Cells and after being used to whole culture plate 1 cleaning and sterilizing, in order to next time use; The opening cultivated above hole 8 is cultivates hole opening 15, and diameter is 2 millimeters, is to cultivate the passage that hole 8 is cultivated in the cell turnover of cultivating in hole 8; To cultivate hole substrate 17 are breezing plate 9 lower surfaces to cultivate at the bottom of hole between 16 part, each can be cultivated hole 8 and connect into breezing plate 9.
Preferred version of the present invention is: layer-stepping Tissue Culture Plate operating process is: when carrying out cell cultures, first by whole culture plate 1 cleaning and sterilizing, then in the gnotobasis of Bechtop, cell culture fluid is filled into and cultivates in plate hole 2, multilayer breezing plate 9 is provided with owing to cultivating in plate hole 2, fill after cultivating plate hole 2, cell and nutrient solution thereof can arrive cultivates each corner in plate hole 2, certainly each cultivation hole 8 is also comprised, then, siphoned away by the cell culture fluid cultivating plate hole 2 preglabellar field with suction pipe, cell can grow in cultivation hole 8.
Beneficial effect of the present invention is, layer-stepping Tissue Culture Plate utilizes breezing plate cultivation plate hole to be divided into multilayer and cultivates face, each cultivation face is designed again multiple cultivation hole, the growth needs supporting plane of cell, because layering adds the design in multiple cultivation hole, expand the area of Growth of Cells, the more known culture plate of the culture area of layer-stepping Tissue Culture Plate is significantly improved, the use of glass and Teflon, culture plate can also be made to be reused, the rear waste namely abandoned resource of known batch cultur plate can be reduced, thus reach the object reducing cell cultures expense.Layer-stepping Tissue Culture Plate makes simple, workable, with low cost, successful.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is the one-piece construction schematic diagram of layer-stepping Tissue Culture Plate of the present invention.
Fig. 2 is the cultivation plate hole vertical section structure schematic diagram of layer-stepping Tissue Culture Plate of the present invention.
Fig. 3 is the breezing plate structural representation of layer-stepping Tissue Culture Plate of the present invention.
Fig. 4 is the support column arrangement schematic diagram of layer-stepping Tissue Culture Plate of the present invention.
Fig. 5 is the cultivation pore structure schematic diagram of layer-stepping Tissue Culture Plate of the present invention.
1. culture plates in figure, 2. cultivate plate hole, 3. cultivate board slot, 4. culture plate cell wall, 5. millipore filtration, 6. plate hole column, 7. breezing plate axis hole, 8. cultivates hole, 9. breezing plate, 10. culture plate hole wall, 11. culture plate vestibules, 12. pillar stiffeners, 13. cultivate hole wall, 14. Teflon layer, and 15. cultivate hole opening, 16. cultivate at the bottom of hole, and 17. cultivate hole substrate.
Embodiment
Embodiment one:
Layer-stepping Tissue Culture Plate by culture plate 1, cultivate plate hole 2, cultivate board slot 3, culture plate cell wall 4, millipore filtration 5, plate hole column 6, breezing plate axis hole 7, cultivate hole 8, breezing plate 9, culture plate hole wall 10, culture plate vestibule 11, pillar stiffener 12, cultivate hole wall 13, Teflon layer 14, cultivate hole opening 15, to cultivate at the bottom of hole 16, cultivate hole substrate 17 and form, it is characterized in that culture plate 1 is made up of glass, top inwardly has a degree of depth to be the cultivation board slot 3 of 5-10 millimeter, in cultivation board slot 3,6 are had to cultivate plate hole 2; Cultivate plate hole 2 upper part to exceed and cultivate bottom board slot 3, so as millipore filtration 5 can wrap cultivate plate hole 2 opening outside the opening cultivating plate hole 2 is wound around after seal; Cultivate plate hole 2 central authorities and have a plate hole column 6, after plate hole column 6 is through breezing plate axis hole 7 and pillar stiffener 12, breezing plate 9 can be superimposed from level to level; Pillar stiffener 12 breezing plate 9 is spaced from each other the glass cylinder of 2 millimeters; At the upper surface of breezing plate 9, between breezing plate axis hole 7 periphery to breezing plate 9 periphery, regularly arranged multiple diameter is 2 millimeters, the degree of depth is the cultivation hole 8 of 2 millimeters, cultivates the main place that hole 8 is Growth of Cells.
In cultivation board slot 3, have 6 diameters to be 3-3.5 centimetre, the degree of depth is the cultivation plate hole 2 of 1.0-1.8 centimetre; Cultivate plate hole 2 upper part and exceed 4-9 millimeter bottom cultivation board slot 3, so that millipore filtration 5 can wrap the opening cultivating plate hole 2, allow the cell cultivated in plate hole 2 both can avoid the pollution of bacterium, freely can obtain oxygen from the outside again, be beneficial to the breathing of cell, prevent anoxic from bringing adverse influence to cultured cells.
The outwardly convex of cultivating around board slot 3 is culture plate cell wall 4, culture plate cell wall 4 upper surface and the opening cultivating plate hole 2 superscribe the surface after millipore filtration 5 in same level, be convenient to packed and transported, the performance attractive in appearance of culture plate during culturing cell, can also be ensured.
Millipore filtration 5 commercially available can ensure that moisture and gas pass through, but can stop the invasion and attack of bacteriophage, on the Bechtop disinfected, seals, cultivate under being then placed on optimal temperature after the opening cultivating plate hole 2 being wound around with millipore filtration 5.
A diameter is had to be vitreous plate hole column 6 of 2 millimeters cultivating plate hole 2 central authorities, after plate hole column 6 is through breezing plate axis hole 7 and pillar stiffener 12, breezing plate 9 can be superimposed from level to level, fill the culture plate vestibule 11 surrounded by culture plate hole wall 10.
The glass cylinder that adjacent two pieces of breezing plates 9 are spaced from each other 2 millimeters by pillar stiffener 12, diameter is 0.5 centimetre, length is 2 millimeters, barrel bore is 2.2 millimeters, and cylindrical wall thickness is 1.4 millimeters; Pillar stiffener 12 is positioned at breezing plate 9 central authorities below, and connects as one with breezing plate 9, and the cylinder bore of pillar stiffener 12 is connected up and down with the vestibule of breezing plate axis hole 7, and internal diameter size is 2.2 millimeters.
The plectane that breezing plate 9 is vitreous diameters is 2.5-3 centimetre, thickness is 2.5-3 millimeter, plectane edge and the distance of cultivating between plate hole 2 wall are 2 millimeters, so that band needs the nutrient solution of culturing cell and injects and cultivate in plate hole 2, the diameter of plectane central authorities is the hole of 2.2 millimeters is breezing plate axis hole 7.
The hole that to cultivate hole 8 are regularly arranged between the upper surface, breezing plate axis hole 7 periphery to breezing plate 9 periphery of breezing plate 9, multiple diameters is 2 millimeters, the degree of depth is 2 millimeters, cultivation hole 8 is main place of Growth of Cells; Each cultivation hole 8 includes and cultivates hole wall 13, Teflon layer 14, cultivate hole opening 15, to cultivate at the bottom of hole 16, cultivate hole substrate 17; Cultivate hole wall 13 and to cultivate at the bottom of hole 16 and jointly surround the inner chamber cultivating hole 8, glass quality, Teflon can be added to breezing plate 9 upper surface according to known loading method and cultivate hole wall 13 and cultivate the surface of at the bottom of hole 16, form the Teflon layer 14 that thickness is 0.1-0.2 millimeter, be conducive to Growth of Cells and after being used to whole culture plate 1 cleaning and sterilizing, in order to next time use; The opening cultivated above hole 8 is cultivates hole opening 15, and diameter is 2 millimeters, is to cultivate the passage that hole 8 is cultivated in the cell turnover of cultivating in hole 8; To cultivate hole substrate 17 are breezing plate 9 lower surfaces to cultivate at the bottom of hole between 16 part, each can be cultivated hole 8 and connect into breezing plate 9.
The operating process of layer-stepping Tissue Culture Plate is: when carrying out cell cultures, first by whole culture plate 1 cleaning and sterilizing, then in the gnotobasis of Bechtop, cell culture fluid is filled into and cultivates in plate hole 2, multilayer breezing plate 9 is provided with owing to cultivating in plate hole 2, fill after cultivating plate hole 2, cell and nutrient solution thereof can arrive cultivates each corner in plate hole 2, certainly each cultivation hole 8 is also comprised, then, siphoned away by the cell culture fluid cultivating plate hole 2 preglabellar field with suction pipe, cell can grow in cultivation hole 8.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should be appreciated that; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; these changes and improvements all fall in the claimed scope of the invention, and application claims protection domain is defined by appending claims equivalent.
Claims (7)
1. layer-stepping Tissue Culture Plate by culture plate, cultivate plate hole, cultivate board slot, culture plate cell wall, millipore filtration, plate hole column, breezing plate axis hole, cultivate hole, breezing plate, culture plate hole wall, culture plate vestibule, pillar stiffener, cultivation hole wall, Teflon layer, cultivation hole opening, cultivate at the bottom of hole, cultivate hole substrate and form, it is characterized in that culture plate is made up of glass, top inwardly has a degree of depth to be the cultivation board slot of 5-10 millimeter, in cultivation board slot, 6 are had to cultivate plate hole; Cultivate plate hole upper part and exceed culture plate trench bottom, so as millipore filtration can wrap cultivate plate hole opening outside the opening cultivating plate hole is wound around after seal; Cultivate plate hole central authorities and have a plate hole column, after plate hole column is through breezing plate axis hole and pillar stiffener, breezing plate can be superimposed from level to level; Pillar stiffener breezing plate is spaced from each other the glass cylinder of 2 millimeters; The hole that to cultivate hole be regularly arranged between the upper surface, breezing plate axis hole periphery to breezing plate periphery of breezing plate, multiple diameter is 2 millimeters, the degree of depth is 2 millimeters, cultivation hole is the main place of Growth of Cells; Each cultivation hole includes and cultivates hole wall, Teflon layer, cultivation hole opening, cultivates at the bottom of hole, cultivates hole substrate; Cultivate hole wall and cultivate the inner chamber jointly surrounding at the bottom of hole and cultivate hole, surface Teflon being loaded into breezing plate upper surface and cultivating hole wall and cultivate at the bottom of hole, form the Teflon layer that thickness is 0.1-0.2 millimeter, be conducive to Growth of Cells and after being used to whole culture plate cleaning and sterilizing, in order to next time use; The opening cultivated above hole is cultivates hole opening, and diameter is 2 millimeters, is to cultivate the passage that hole is cultivated in the cell turnover of cultivating in hole; To cultivate hole substrate be breezing plate lower surface between cultivating at the bottom of hole part, each can be cultivated hole and connect into breezing plate.
2. layer-stepping Tissue Culture Plate according to claim 1, is characterized in that have 6 diameters to be 3-3.5 centimetre, the degree of depth is the cultivation plate hole of 1.0-1.8 centimetre in cultivation board slot; Cultivate plate hole upper part and exceed culture plate trench bottom 4-9 millimeter, so that millipore filtration can wrap the opening cultivating plate hole, allow the cell cultivated in plate hole both can avoid the pollution of bacterium, freely can obtain oxygen from the outside again, be beneficial to the breathing of cell, prevent anoxic from bringing adverse influence to cultured cells.
3. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that the outwardly convex of cultivating around board slot is culture plate cell wall, culture plate cell wall upper surface and the opening cultivating plate hole superscribe the surface after millipore filtration in same level, be convenient to packed and transported, the performance attractive in appearance of culture plate during culturing cell, can also be ensured.
4. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that millipore filtration to ensure that moisture and gas pass through, but the invasion and attack of bacteriophage can be stoped, on the Bechtop disinfected, seal after the opening cultivating plate hole being wound around with millipore filtration, cultivate under being then placed on optimal temperature.
5. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that having a diameter to be vitreous plate hole column of 2 millimeters cultivating plate hole central authorities, after plate hole column is through breezing plate axis hole and pillar stiffener, breezing plate can be superimposed from level to level, fill the culture plate vestibule surrounded by culture plate hole wall.
6. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that the glass cylinder that adjacent two pieces of breezing plates are spaced from each other 2 millimeters by pillar stiffener, diameter is 0.5 centimetre, length is 2 millimeters, barrel bore is 2.2 millimeters, and cylindrical wall thickness is 1.4 millimeters; Pillar stiffener is positioned at below breezing plate central authorities, and connects as one with breezing plate, and the cylinder bore of pillar stiffener is connected up and down with the vestibule of breezing plate axis hole, and internal diameter size is 2.2 millimeters.
7. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that the plectane that breezing plate be vitreous diameter is 2.5-3 centimetre, thickness is 2.5-3 millimeter, distance between plectane edge and culture plate hole wall is 2 millimeters, so that band needs the nutrient solution of culturing cell and injects and cultivate in plate hole, the diameter of plectane central authorities is the hole of 2.2 millimeters is breezing plate axis hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210410253.7A CN103773683B (en) | 2012-10-17 | 2012-10-17 | Layer-stepping Tissue Culture Plate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210410253.7A CN103773683B (en) | 2012-10-17 | 2012-10-17 | Layer-stepping Tissue Culture Plate |
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| CN103773683A CN103773683A (en) | 2014-05-07 |
| CN103773683B true CN103773683B (en) | 2015-11-25 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105505745A (en) * | 2016-01-15 | 2016-04-20 | 北京酷搏科技有限公司 | Perforated plate and perforated plate assembly including same |
| CN109097274A (en) * | 2017-06-20 | 2018-12-28 | 中国科学院大连化学物理研究所 | The standardized high-throughput three-dimensional cell preparation facilities of one kind and its application |
| CN109187937B (en) * | 2018-08-20 | 2020-03-13 | 河南科技大学第一附属医院 | Capillary type cancer cell culture detection growth device |
| CN114733472B (en) * | 2022-04-24 | 2024-05-03 | 中国科学院长春应用化学研究所 | Reaction kettle suitable for multistage reaction |
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| CN101978041B (en) * | 2008-01-25 | 2014-07-30 | 康宁股份有限公司 | Limited access multi-layer cell culture system |
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| CN2186755Y (en) * | 1994-03-25 | 1995-01-04 | 中国科学院成都生物研究所 | Multipurpose culture plate |
| US5972694A (en) * | 1997-02-11 | 1999-10-26 | Mathus; Gregory | Multi-well plate |
| CN2601737Y (en) * | 2003-03-06 | 2004-02-04 | 山东大学 | Multifunctional ware stand |
| CN2887882Y (en) * | 2005-08-04 | 2007-04-11 | 中国人民解放军第四军医大学 | Culturing and cleaning dish special for cell growing on cover glass |
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| US20100055790A1 (en) * | 2006-11-14 | 2010-03-04 | Simon Eric M | Cell culture apparatus and associated methods |
| CN201195726Y (en) * | 2008-02-19 | 2009-02-18 | 中国航天员科研训练中心 | Combined cell culture bottle |
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| CN103773683A (en) | 2014-05-07 |
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