CN105505745A - Perforated plate and perforated plate assembly including same - Google Patents
Perforated plate and perforated plate assembly including same Download PDFInfo
- Publication number
- CN105505745A CN105505745A CN201610029335.5A CN201610029335A CN105505745A CN 105505745 A CN105505745 A CN 105505745A CN 201610029335 A CN201610029335 A CN 201610029335A CN 105505745 A CN105505745 A CN 105505745A
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- CN
- China
- Prior art keywords
- porous plate
- reacting hole
- hole
- plate
- edge
- Prior art date
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Abstract
The invention provides a perforated plate and a perforated plate assembly including the same. The perforated plate comprises a plate body, wherein 96 reaction holes with same structure are formed in the plate body, and the volume of each reaction hole ranges from 25 microliters to 40 microliters. By virtue of the technical scheme provided by the invention, the problems of large liquid volume, high power consumption and long reaction time in the test of the prior art can be solved.
Description
Technical field
The present invention relates to high throughput testing technical field, in particular to a kind of porous plate and the porous plate assembly with it.
Background technology
Porous plate is by the identical opening goods that obtain of repeated arrangement in the two-dimensional direction, is generally used for contain fluid sample.Porous plate is good due to the consistence between its hole, makes easy and simple to handle, and therefore, porous plate has a wide range of applications in high throughput testing reaction and high flux screening.
When the porous plate using prior art to provide carries out PCR reaction test, because the liquid volume participating in alternating temperature reaction is large, cause watt consumption comparatively large, long reaction time.And causing when reading fluorescent signal, reading speed is comparatively slow, stability is low.
Summary of the invention
The invention provides a kind of porous plate and have its porous plate assembly, large to solve in prior art the liquid volume when testing, watt consumption is large, the problem of long reaction time.
According to an aspect of the present invention, provide a kind of porous plate, porous plate comprises plate body, plate body is provided with the reacting hole that 96 structures are identical, and the volume of each reacting hole is in the scope of 25uL to 40uL.
Further, the diameter of the opening of reacting hole is greater than the diameter of the bottom surface of reacting hole, and reacting hole is extended to bottom surface gradually by opening with reducing, and the hole wall of reacting hole and the angle of horizontal plane are between 80 degree to 85 degree.
Further, the thickness of the hole wall of reacting hole is between 0.26mm to 0.32mm.
Further, the thickness at the bottom of hole of reacting hole is between 0.4mm to 0.6mm.
Further, porous plate also comprises 96 bulge loops, and bulge loop is arranged on the opening edge edge of reacting hole, and bulge loop is higher than the surface of plate body, and bulge loop and reacting hole are arranged correspondingly.
Further, reacting hole array is arranged on plate body, and plate body has the first edge and Second Edge edge that are oppositely arranged, and the first edge is not identical with the closest range of reacting hole with Second Edge edge with the closest range of reacting hole.
According to a further aspect in the invention, provide a kind of porous plate assembly, porous plate assembly comprises porous plate and orifice plate base, and orifice plate base has holding tank, and porous plate is arranged in holding tank, and porous plate is the above-mentioned porous plate provided.
Further, porous plate comprises plate body, and 96 reacting hole arrays are arranged on plate body, and orifice plate base is provided with separation trough, and the medullary ray of the array that separation trough and 96 reacting holes are formed on the same line.
Further, orifice plate base is provided with relief portion, and relief portion is communicated with holding tank.
Further, orifice plate base is provided with colour recognition portion, the reacting hole in the corresponding porous plate in colour recognition portion is arranged in holding tank.
Applying technical scheme of the present invention, by being arranged in the scope of 25uL to 40uL by the volume of reacting hole, so can reduce the volume of reacting hole, and then the reaction volume detecting liquid can be reduced, be convenient to make in test detection liquid rapid temperature increases and react.Use this porous plate to test, can heat-up time be shortened, reduce heating power consumption, improve test efficiency.
Accompanying drawing explanation
The Figure of description forming a application's part is used to provide a further understanding of the present invention, and schematic description and description of the present invention, for explaining the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings:
Fig. 1 shows the structural representation of the porous plate provided according to the embodiment of the present invention;
Fig. 2 shows the structural representation of reacting hole in Fig. 1;
Fig. 3 shows the sectional view that in Fig. 1, two porous plates are stacked;
Fig. 4 shows the front view of porous plate in Fig. 1;
Fig. 5 shows the scale diagrams of porous plate in Fig. 1;
Fig. 6 shows the structural representation of the porous plate assembly provided according to the embodiment of the present invention;
Fig. 7 shows the structural representation of Fig. 6 aperture plate base;
Fig. 8 shows the scale diagrams of Fig. 6 aperture plate base;
Fig. 9 shows structural representation Fig. 6 aperture plate base being arranged colour recognition portion.
Wherein, above-mentioned accompanying drawing comprises the following drawings mark:
10, plate body; 20, reacting hole; 30, bulge loop; 100, porous plate; 200, orifice plate base; 210, holding tank; 220, separation trough; 230, relief portion.
Embodiment
It should be noted that, when not conflicting, the embodiment in the application and the feature in embodiment can combine mutually.Below with reference to the accompanying drawings and describe the present invention in detail in conjunction with the embodiments.
As shown in Figures 1 to 5, the embodiment of the present invention is a kind of porous plate, and this porous plate comprises plate body 10.Wherein, plate body 10 is provided with the reacting hole 20 that 96 structures are identical, the volume of each reacting hole 20 is all arranged in the scope of 25uL to 40uL.96 reacting hole 20 arrays are arranged on plate body 10, and particularly, reacting hole 20 is the matrix that 8 row 12 arrange in the present embodiment.The small-sized porous plate that the application of the invention provides, the spacing between reacting hole 20 can be reduced, and then the volume of test tool can be reduced, and, use little volume reacting hole 20 can reduce the volume of tracer liquid, reduce heat-up time, reduce heating power consumption, and the time that tracer liquid reaches desired temperature can be shortened, improve bulk testing efficiency.
Alternatively, the volume of this reacting hole 20 is set to 35uL, and it can allow the detection liquid of 0.5uL to 20uL to participate in reaction.50% to 90% is reduced compared to the volume in common response hole.The reacting hole 20 applying this volume is tested, and can improve the heat-up rate detecting liquid, greatly shortens the bulk testing time.
Fig. 2 shows the concrete structure of reacting hole, the diameter of the opening of this reacting hole 20 is greater than the diameter of the bottom surface of reacting hole 20, and reacting hole 20 is extended to direction, bottom surface gradually by opening with reducing, the hole wall of reacting hole 20 and the angle α of horizontal plane are between 80 degree to 85 degree.Tracer liquid so can be made not easily to remain on hole wall, and liquid residue is low.Further, the hole wall at this angle of inclination is adopted to be conducive to, when carrying out fluorescent PCR test, utilizing the fluorescence intensity in all reacting holes 20 of the disposable reading of method of face battle array imaging.Alternatively, the angle of this angle α is 83 degree.
The less heat conductivility of pore wall thickness of reacting hole 20 is better, but the less physical strength that also can reduce porous plate of pore wall thickness.Particularly, in the present embodiment, be arranged between 0.26mm to 0.32mm by the thickness of the hole wall of reacting hole 20, the thickness at the bottom of hole is arranged between 0.4mm to 0.6mm.So be conducive to the conduction of heat in alternating temperature reaction, shorten heat balance time and the total reaction time of tracer liquid, and the physical strength of porous plate entirety can be ensured.
In the present embodiment, this porous plate is also provided with 96 bulge loops 30.Particularly, this bulge loop 30 is arranged on the opening edge of reacting hole 20, and bulge loop 30 is higher than the surface of plate body 10, and bulge loop 30 and reacting hole 20 are arranged correspondingly.Shrouding membrane closure reacting hole 20 easy to use like this, not easily makes liquid hanker evaporating adding.Optionally, the height of this bulge loop 30 and thickness are respectively 0.55mm and 0.28mm.
For the ease of the arragement direction in recognition reaction hole 20, reduce staff's error rate, plate body 10 is also provided with recognition structure.Wherein, recognition structure has multiple, such as, can arrange coordinate word on plate body 10, and for Fig. 1 or Fig. 5, horizontally-arrangedly arrange numeral, tandem arranges letter.So be convenient to the arrangement mode of combing reacting hole 20.In addition, fillet can also be set on plate body 10, so that the initiating terminal in staff's recognition reaction hole.The first edge and Second Edge edge that plate body 10 is oppositely arranged can also be chosen, make the first edge not identical with the closest range of reacting hole 20 with Second Edge edge with the closest range of reacting hole 20, to form recognition structure.Particularly, the closest range of the first edge and reacting hole 20 can be greater than the closest range of Second Edge edge and reacting hole 20, put so that multiple porous plate is unified.
Further, plate body 10 is provided with salient point, salient point is evenly distributed on the interval of reacting hole 20, to make 96 reacting holes 20 be divided into the region of formed objects, and then is convenient to staff and divides 96 reacting holes 20.
Wherein, porous plate is made up of the polypropylene material of low Poison.Apply the accuracy that this porous plate is conducive to improving fluorescent quantitative PCR result, and the physical strength of porous plate entirety can be ensured.
The edge of plate body 10 is set to curved, so can be convenient to picking and placeing of porous plate.Further, as shown in Figure 3, groove is provided with in plate body 10 bottom surface and near edge, so that staff captures.
As shown in Figures 6 to 9, in another embodiment provided by the invention, provide a kind of porous plate assembly, this porous plate assembly comprises porous plate 100 and orifice plate base 200, and orifice plate base 200 has holding tank 210, and porous plate 100 correspondence is arranged in holding tank 210.
Particularly, porous plate 100 comprises plate body 10, and 96 reacting hole 20 arrays are arranged on plate body 10, orifice plate base 200 are provided with separation trough 220, the medullary ray of the array that separation trough 220 and 96 reacting holes 20 are formed on the same line, so that 96 reacting hole 20 symmetries are separated into two portions.Alternatively, this separation trough 220 also may correspond to other positions that reacting hole 20 is arranged on orifice plate base 200 and separates 96 reacting holes 20, such as, is arranged on 1/3rd places or 1/4th places etc. of reacting hole 20.
In the present embodiment, orifice plate base 200 is also provided with relief portion 230, wherein, relief portion 230 is communicated with holding tank 210.So after porous plate 100 is placed on holding tank 210, porous plate 100 can be taken out by relief portion 230 by staff from holding tank 210.Alternatively, this relief portion 230 can be set to square opening.
Wherein, orifice plate base 200 is also provided with colour recognition portion, the reacting hole 20 in the corresponding porous plate 100 in this colour recognition portion is arranged in holding tank 210.So be conducive to staff and separate reacting hole 20, as described in Figure 9, by arranging 4 different colors on orifice plate base 200, reacting hole 20 correspondence can be divided into 4 regions, so be conducive to staff and identifying this 4 regions.Alternatively, user can arrange as required and organize color more, to meet the user demand in its multiple region.
In the present embodiment, orifice plate base 200 also arranges recognition structure, particularly, fillet or corner cut can be set in one end of orifice plate base 200 so that identify orifice plate base 200 setting direction.Further, this orifice plate base 200 is made by aluminum alloy materials, and aluminum alloy materials thermal conductivity is high, keeps low temperature when being so convenient to add sample on porous plate 100.
By porous plate assembly provided by the invention, the size of test tool can be reduced, reduce the reaction volume detecting liquid, thus the heat-up time that can reduce when reacting, detection liquid is promoted to rapidly temperature required, and then can test period be shortened, improve test efficiency.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a porous plate, it is characterized in that, described porous plate comprises plate body (10), described plate body (10) is provided with the reacting hole (20) that 96 structures are identical, and the volume of each described reacting hole (20) is in the scope of 25uL to 40uL.
2. porous plate according to claim 1, it is characterized in that, the diameter of the opening of described reacting hole (20) is greater than the diameter of the bottom surface of described reacting hole (20), described reacting hole (20) is extended to described bottom surface gradually by described opening with reducing, and the hole wall of described reacting hole (20) and the angle of horizontal plane are between 80 degree to 85 degree.
3. porous plate according to claim 1, is characterized in that, the thickness of the hole wall of described reacting hole (20) is between 0.26mm to 0.32mm.
4. porous plate according to claim 1, is characterized in that, the thickness at the bottom of hole of described reacting hole (20) is between 0.4mm to 0.6mm.
5. porous plate according to claim 1, it is characterized in that, described porous plate also comprises 96 bulge loops (30), described bulge loop (30) is arranged on the opening edge edge of described reacting hole (20), and described bulge loop (30) is higher than the surface of described plate body (10), described bulge loop (30) and described reacting hole (20) are arranged correspondingly.
6. porous plate according to claim 1, it is characterized in that, described reacting hole (20) array is arranged on described plate body (10), described plate body (10) has the first edge and Second Edge edge that are oppositely arranged, and described first edge is not identical with the closest range of described reacting hole (20) with described Second Edge edge with the closest range of described reacting hole (20).
7. a porous plate assembly, described porous plate assembly comprises porous plate (100) and orifice plate base (200), described orifice plate base (200) has holding tank (210), described porous plate (100) is arranged in described holding tank (210), it is characterized in that, described porous plate (100) porous plate according to any one of claim 1 to 6.
8. porous plate assembly according to claim 7, it is characterized in that, described porous plate (100) comprises plate body (10), 96 reacting hole (20) arrays are arranged on described plate body (10), described orifice plate base (200) is provided with separation trough (220), and the medullary ray of the array that described separation trough (220) is formed with 96 described reacting holes (20) on the same line.
9. porous plate assembly according to claim 8, is characterized in that, described orifice plate base (200) is provided with relief portion (230), and described relief portion (230) is communicated with described holding tank (210).
10. porous plate assembly according to claim 9, it is characterized in that, described orifice plate base (200) is provided with colour recognition portion, and the reacting hole (20) in the corresponding described porous plate (100) in described colour recognition portion is arranged in described holding tank (210).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610029335.5A CN105505745A (en) | 2016-01-15 | 2016-01-15 | Perforated plate and perforated plate assembly including same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610029335.5A CN105505745A (en) | 2016-01-15 | 2016-01-15 | Perforated plate and perforated plate assembly including same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105505745A true CN105505745A (en) | 2016-04-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610029335.5A Pending CN105505745A (en) | 2016-01-15 | 2016-01-15 | Perforated plate and perforated plate assembly including same |
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| CN (1) | CN105505745A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109661270A (en) * | 2016-07-15 | 2019-04-19 | 国立研究开发法人产业技术综合研究所 | Porous plate |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999051347A2 (en) * | 1998-04-06 | 1999-10-14 | Pharmacopeia, Inc. | Cell culture chamber for multiple sample substrates |
| US20090061513A1 (en) * | 2007-05-15 | 2009-03-05 | Picovitro Ab | Cell sorting and cell cultivation methods |
| CN103773683A (en) * | 2012-10-17 | 2014-05-07 | 安徽农业大学 | Layered-type cell culture plate |
-
2016
- 2016-01-15 CN CN201610029335.5A patent/CN105505745A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999051347A2 (en) * | 1998-04-06 | 1999-10-14 | Pharmacopeia, Inc. | Cell culture chamber for multiple sample substrates |
| US20090061513A1 (en) * | 2007-05-15 | 2009-03-05 | Picovitro Ab | Cell sorting and cell cultivation methods |
| CN103773683A (en) * | 2012-10-17 | 2014-05-07 | 安徽农业大学 | Layered-type cell culture plate |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109661270A (en) * | 2016-07-15 | 2019-04-19 | 国立研究开发法人产业技术综合研究所 | Porous plate |
| US10967377B2 (en) | 2016-07-15 | 2021-04-06 | National Institute Of Advanced Industrial Science And Technology | Multi-well plate |
| CN109661270B (en) * | 2016-07-15 | 2021-12-28 | 国立研究开发法人产业技术综合研究所 | Perforated plate |
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Application publication date: 20160420 |