CN211471437U - Culture assembly - Google Patents
Culture assembly Download PDFInfo
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- CN211471437U CN211471437U CN201922084033.XU CN201922084033U CN211471437U CN 211471437 U CN211471437 U CN 211471437U CN 201922084033 U CN201922084033 U CN 201922084033U CN 211471437 U CN211471437 U CN 211471437U
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Abstract
The embodiment of the utility model provides a cultivate subassembly for the sample is cultivateed, cultivate the subassembly and include: a culture plate having a plurality of culture units for receiving to culture the sample; and the auxiliary bracket is arranged on the culture plate and is provided with a plurality of limiting units which are used for extending into the culture unit and are matched with the culture unit so as to limit the sample at the preset position of the culture unit. The embodiment of the utility model provides a beneficial effect who possesses includes simple structure, easily preparation, can effectively spacing sample in the position department of predetermineeing of cultivateing the unit moreover.
Description
Technical Field
The embodiment of the utility model provides a biological engineering field is related to, in particular to cultivate subassembly.
Background
The existing 3D organoids are mostly cultured in the porous plate, the 3D organoids are easy to stay in the edge area of the hole in the porous plate, and the position is not fixed. Particularly, in the case of a micro-electrode array (MEA) culture plate, a micro-electrode is positioned at the center of the multi-well plate, when electrophysiological activity detection is performed, a 3D type organ needs to be attached to the center of the well, and the 3D type organ is difficult to fix at the center of the multi-well plate with a micro-electrode area without auxiliary materials.
SUMMERY OF THE UTILITY MODEL
The embodiment of the utility model provides a can effectively fix sample in the cultivation subassembly of predetermineeing position department.
In order to solve the technical problem, the embodiment of the utility model provides a cultivate subassembly for the sample is cultivateed, cultivate the subassembly and include:
a culture plate having a plurality of culture units for receiving to culture the sample; and
the auxiliary support is arranged on the culture plate and provided with a plurality of limiting units, the limiting units are used for extending into the culture unit and are matched with the culture unit to limit the sample at the preset position of the culture unit.
Preferably, a portion of said culture plate extends vertically outwardly to form said culture unit.
Preferably, the culture unit is provided with a holding chamber with one open end, and the sample is positioned in the holding chamber.
Preferably, the culture unit has a cylindrical shape.
Preferably, the auxiliary stand includes a base plate, and a part of the base plate extends perpendicularly to the direction of the culture plate to form the position limiting unit.
Preferably, the limiting unit is a hollow cylindrical body.
Preferably, the auxiliary support is stacked on the culture plate, the limiting unit correspondingly extends into the culture unit and is used for being matched with the culture unit to form a limiting cavity for containing and limiting the sample, and the preset position is located in the limiting cavity.
Preferably, a gap is formed between the bottom of the limiting unit and the culture unit.
Can learn based on the disclosure of above-mentioned embodiment, the beneficial effect that the embodiment of the utility model provides a possesses includes simple structure, easily preparation, can effectively spacing sample in the preset position department of cultivateing the unit moreover.
Drawings
FIG. 1 is a schematic view of the structure of a culture assembly in an embodiment of the present application.
Fig. 2 is a schematic structural diagram of an auxiliary support in an embodiment of the present application.
Reference numerals:
1-culture plate; 2-an auxiliary support; 3-a culture unit; 4-a limiting unit; 5-a substrate; 6-sample
Detailed Description
The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings, but not intended to limit the invention thereto.
It will be understood that various modifications may be made to the embodiments disclosed herein. The following description is, therefore, not to be taken in a limiting sense, but is made merely as an exemplification of embodiments. Other modifications will occur to those skilled in the art within the scope and spirit of the disclosure.
The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate embodiments of the disclosure and, together with a general description of the disclosure given above, and the detailed description of the embodiments given below, serve to explain the principles of the disclosure.
These and other characteristics of the invention will become apparent from the following description of a preferred form, given as a non-limiting example, with reference to the accompanying drawings.
It is also understood that, although the present invention has been described with reference to some specific examples, a person of skill in the art shall certainly be able to achieve many other equivalent forms of the invention, having the characteristics as set forth in the claims and hence all coming within the field of protection defined thereby.
The above and other aspects, features and advantages of the present disclosure will become more apparent in view of the following detailed description when taken in conjunction with the accompanying drawings.
Specific embodiments of the present disclosure are described hereinafter with reference to the accompanying drawings; however, it is to be understood that the disclosed embodiments are merely examples of the disclosure that may be embodied in various forms. Well-known and/or repeated functions and structures have not been described in detail so as not to obscure the present disclosure with unnecessary or unnecessary detail. Therefore, specific structural and functional details disclosed herein are not to be interpreted as limiting, but merely as a basis for the claims and as a representative basis for teaching one skilled in the art to variously employ the present disclosure in virtually any appropriately detailed structure.
The specification may use the phrases "in one embodiment," "in another embodiment," "in yet another embodiment," or "in other embodiments," which may each refer to one or more of the same or different embodiments in accordance with the disclosure.
The embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
As shown in fig. 1 and 2, the present invention provides a culture assembly for culturing a sample 6, the culture assembly comprising:
a culture plate 1 having a plurality of culture units 3 for accommodating samples 6 to be cultured; and
and an auxiliary support 2 disposed on the culture plate 1, wherein the auxiliary support 2 has a plurality of position-limiting units 4 for extending into the culture unit 3 and cooperating with the culture unit 3 to limit the sample 6 at a predetermined position of the culture unit 3.
The embodiment of the utility model provides a beneficial effect who possesses includes simple structure, easily preparation, can effectively spacing sample 6 in the position department of predetermineeing of cultivateing unit 3 moreover, the high flux of being convenient for detects. Especially when sample 6 is biological sample 6, and need carry out the electrophysiological activity to this biological sample 6 and detect, fix spacing biological sample 6 through the culture assembly in this application embodiment, can carry out effective restriction to the position of biological sample 6, guarantee going on smoothly of electrophysiological activity detection to and the rate of accuracy of detection data.
Specifically, as shown in the figure, the culture unit 3 in the present embodiment is integrally formed with the culture plate 1, but may be provided independently. In operation, each culture unit 3 may be formed by extending a portion of the plate 1 vertically outward. Wherein, cultivate unit 3 and be the column, the shape differs, and cultivate unit 3 in this embodiment is cylindricly to have the open holding chamber of one end, cultivate unit 3 and be uncovered towards the one end of cultivateing board 1 promptly, sample 6 is located this holding chamber.
Further, the auxiliary stand 2 in the present embodiment includes a base plate 5, and the position limiting unit 4 is integrally formed with the base plate 5, but may be separately provided. In operation, a part of the substrate 5 may be extended vertically towards the direction of the culture plate 1 to form the limiting unit 4, and in this embodiment, the fixing limiting unit 4 is a hollow cylinder, specifically, a cylinder, or a cylinder with other shapes. In addition, preferably, the limiting units 4 of the auxiliary stent 2 in this embodiment are all made of an ultralow adsorption hydrophobic material, so as to prevent the biological sample 6 from adhering to the limiting units 4.
In practice, for example, a plurality of position-limiting units 4 on the auxiliary support 2 may be arranged in a matrix type, such as 6x8,8x12, and the number of the culture units 3 and the position-limiting units 4 may be the same and one-to-one, or may be different, such as smaller than the number of the culture units 3, and a plurality of auxiliary supports 2 may be arranged on one culture plate 1. Further, for example, when culturing the biological sample 6, the auxiliary support 2 is firstly stacked on the culture plate 1, so that the position-corresponding limiting unit 4 correspondingly extends into the culture unit 3, and thus the limiting unit 4 and the corresponding culture unit 3 cooperate to form a limiting chamber for accommodating and limiting the biological sample 6, and the preset position is located in the limiting chamber, and the preset position is not unique, such as the center of the limiting chamber. After the limiting unit 4 extends into the culture unit 3, a gap is required between the bottom of the limiting unit 4 and the culture unit 3, and the gap is preferably 100-200 micrometers, that is, the limiting unit 4 is suspended in the culture unit 3. The gap is arranged so that when the culture assembly in this embodiment is used for culturing a biological sample 6, the culture medium inside and outside the limiting unit 4 can be fully exchanged, and the biological sample 6 can be ensured to obtain sufficient nutrients, oxygen and carbon dioxide.
The above embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and the protection scope of the present invention is defined by the claims. Various modifications and equivalents of the invention can be made by those skilled in the art within the spirit and scope of the invention, and such modifications and equivalents should also be considered as falling within the scope of the invention.
Claims (8)
1. An incubation assembly for specimen incubation, the incubation assembly comprising:
a culture plate having a plurality of culture units for receiving to culture the sample; and
the auxiliary support is arranged on the culture plate and provided with a plurality of limiting units, the limiting units are used for extending into the culture unit and are matched with the culture unit to limit the sample at the preset position of the culture unit.
2. The culture assembly of claim 1, wherein a portion of the culture plate extends vertically outward to form the culture unit.
3. The culturing assembly of claim 1, wherein the culturing unit has a receiving chamber open at one end, the sample being located within the receiving chamber.
4. The culture assembly of claim 3, wherein the culture unit is cylindrical.
5. The culture assembly of claim 1, wherein the auxiliary support comprises a base plate, and a portion of the base plate extends perpendicularly towards the culture plate to form the spacing unit.
6. The culture assembly of claim 5, wherein the spacing unit is a hollow cylinder.
7. The culture assembly of claim 5, wherein the auxiliary support is stacked on the culture plate, the limiting unit correspondingly extends into the culture unit and is used for cooperating with the culture unit to form a limiting chamber for accommodating and limiting the sample, and the preset position is located in the limiting chamber.
8. The culture assembly of claim 1, wherein a gap is provided between the bottom of the spacing unit and the culture unit.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201922084033.XU CN211471437U (en) | 2019-11-27 | 2019-11-27 | Culture assembly |
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CN201922084033.XU CN211471437U (en) | 2019-11-27 | 2019-11-27 | Culture assembly |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113941378A (en) * | 2021-10-14 | 2022-01-18 | 浙江大学 | Multi-cavity electrophysiological micro-nano detection-based neural organoid chip and detection method |
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2019
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113941378A (en) * | 2021-10-14 | 2022-01-18 | 浙江大学 | Multi-cavity electrophysiological micro-nano detection-based neural organoid chip and detection method |
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