CN203048950U - Hanging multicell co-culture device - Google Patents
Hanging multicell co-culture device Download PDFInfo
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- CN203048950U CN203048950U CN 201320040511 CN201320040511U CN203048950U CN 203048950 U CN203048950 U CN 203048950U CN 201320040511 CN201320040511 CN 201320040511 CN 201320040511 U CN201320040511 U CN 201320040511U CN 203048950 U CN203048950 U CN 203048950U
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Abstract
The utility model discloses a hanging cell co-culture device which comprises a culture dish base and a culture dish cover, wherein the culture dish base is provided with a round base plane and a side wall formed by extending upwards from the edge of the base plane; the inner cavity of the culture dish is at least provided with one open hanging culture inner cylinder; the hanging culture inner cylinders comprise lower bottoms made of light-permeability materials, and side walls formed by extending upwards from the peripheries of the lower bottoms and made of permeable membrane materials; hanging brackets are arranged in the hanging culture inner cylinders; and the hanging culture inner cylinders are fixedly arranged at the inner side wall of the culture dish base by the hanging brackets in a hanging manner. The hanging cell co-culture device is for systematization and precision of a cell in-vitro culture system, is practical in function, simple, convenient, and high in operability, and can be used for realizing multiple combination culture of a multicell system and a gas-liquid-solid phase system, simulating in-vitro multicell interaction and multiplication processes more truly, and facilitating integrated system research on cell interaction of a complete biological system.
Description
Technical field
The utility model relates to cell culture apparatus, relates in particular to a kind of cellulous co-culture device.
Background technology
Cell cultures is cell to be in grow under the vitro conditions and to breed, and can observe morphological structure of viable cell etc. in culturing process, monitoring and data logging.Cells in vitro is cultivated can adopt single culture mode or various kinds of cell co-cultivation mode.
Cell culture apparatus commonly used is mainly monoclonal gas-liquid phase plane and cultivates, and its unicity and stationarity have limited the multidirectional development of cell cultures, can not satisfy the further investigation to complex systems such as various kinds of cell interphase interactions.Though also have commercial co-culture device at present, exist to be prone to crossed contamination between the different cells and problem such as to mix.Granted publication number is CN202181306, the day for announcing is on April 4th, 2012, denomination of invention discloses a kind of many cells co-culture device for the Chinese utility model patent of " various kinds of cell is cultivated support and various kinds of cell co-culture device altogether ", it is provided with one and cultivates support, this support comprises stent cover and the bracing frame that is connected with stent cover, has fixed orifices on the stent cover.Stent cover edge is connected with bracing frame.Cultivate support and put into culture dish, the fixed orifices of cultivating support is used for hanging cultivates cell, and each cultivates the cultivation that cell is used for a kind of cell.Then this culture dish, cultivate support and suspension type altogether and cultivate the culture system that cell becomes a various kinds of cell co-cultivation.Various cells grow in respectively in different the cultivation cell or culture dish.Be provided with the aperture that varies in size in the bottom of cultivating cell, can allow liquid freely to pass through, and cell can not pass through.This co-culture device is suitable for the common training method of some cell.And, be difficult to hold the cultivation of the cell that inapplicable volume is less for the size setting in aperture.
The utility model content
The purpose of this utility model is to remedy the deficiencies in the prior art, the many cells co-culture device of provide a kind of function practicality, strong operability, can realize many cells system, the Multiple Combination of the solid heterogeneous system of gas-liquid being cultivated.
To achieve these goals, the utility model has adopted following technical scheme: a kind of suspension type cell co-culture device, comprise culture dish base and culture dish lid, described culture dish base has the circular base plane and extends upward the sidewall of formation from the edge, baseplane, described culture dish base intracavity is provided with a uncovered suspension at least and cultivates inner core, described suspension is cultivated inner core and is comprised going to the bottom and extend upward the sidewall of being made by the permeable membrane material that forms from the periphery of going to the bottom by the manufacturing of translucidus material, described suspension is cultivated inner core and is provided with suspension holdfast, hangs the cultivation inner core and is fixed in culture dish base inner side-wall by the suspension holdfast suspension.Hang the cultivation inner core culture dish is divided into different culture space, by the exchange between permeable membrane sidewall realization cytokine and the metabolite, each culture space all has different cell growths, can take out and insert or change different suspensions arbitrarily according to the cultivation needs and cultivate inner core.
Described suspension type cell co-culture device is provided with the suspension of two or more different diameters and cultivates inner core, and the suspension that diameter is little is cultivated inner core and is fixed on the big suspension cultivation inner core inner side-wall of diameter by the suspension holdfast suspension.
The height that inner core is cultivated in the suspension of described two or more different diameters is superposition successively from the inside to the outside.
Described suspension holdfast is made of three suspension arms that are distributed in the upper ledge periphery.
Preferably, described suspension arm is length-adjustable scalable suspension arm, therefore hangs and cultivates culture dish base or the suspension cultivation inner core sidewall that inner core can hang on different diameter.
Described culture dish base and culture dish lid are made by the translucidus material.
The utility model is at systematize and the precision of cells in vitro culture system, set up the common cultivation formula cell culture apparatus of novel practical, the function practical simplicity, workable, can realize the Multiple Combination cultivation of many cells system, the solid heterogeneous system of gas-liquid, more the external many cells of real simulated interact and amplification procedure, are convenient to total system research is carried out in the cell interaction of complete living things system.
Description of drawings
Fig. 1 is the utility model one-piece construction synoptic diagram.
Fig. 2 is the decomposing schematic representation of culture dish.
Wherein, culture dish base 1.00 first hangs and cultivates inner cores 1.10 suspension arms 1.11 sidewalls 1.12 and go to the bottom and 1.13 second hang and cultivate inner cores 1.20 suspension arms 1.21 sidewalls 1.22 upper bases 1.23 culture dish lid 2.00
Embodiment
As shown in Figures 1 and 2, suspension type many cells co-culture device of the present utility model comprises culture dish 1.00 and culture dish lid 2.00.Culture dish 1.00 and culture dish lid 2.00 are made by the translucidus material, can be used for adherent and suspension cell culture.Culture dish 1.00 has a circular base plane and extends upward the sidewall of formation from the edge, baseplane.The culture dish inner chamber is provided with several and hangs the cultivation inner core.Consider from practical application, be advisable with cylindrical inner core.In this example, be provided with first of different diameter and hang cultivation inner core 1.10 and the second suspension cultivation inner core 1.20.The second suspension cultivation inner core 1.20 of path is positioned at first of big footpath and hangs cultivation inner core 1.10 inner chambers, and it is highly less than the first suspension cultivation inner core, and the mode nested with concentric(al) circles arranges.
First hang to cultivate inner core 1.10 goes to the bottom 1.13 and extend upward the sidewall 1.12 by the manufacturing of permeable membrane material that forms from the periphery of going to the bottom and constitute by the manufacturing of translucidus material.First hangs cultivation inner core top is provided with suspension holdfast, and this suspension holdfast is to be made of the circumferential uniform actinoid suspension arm in three edges, and the first suspension cultivation inner core hangs by suspension holdfast 1.11 and is fixed in the culture dish base intracavity.Second hang to cultivate inner core 1.20 goes to the bottom 1.23 and extend upward the sidewall 1.22 by the manufacturing of permeable membrane material that forms from the periphery of going to the bottom and constitute by the manufacturing of translucidus material.Second hangs the cultivation inner core is fixed in tube inner chamber in the first suspension cultivation by suspension holdfast 1.21 suspensions.
Adopt two suspension cultivation inner cores that the culture dish base intracavity is separated into three cultivation chambers in this example and can carry out the common cultivation of three kinds of cells simultaneously.In the practical application, more suspension also can be set cultivate inner core, form more chambers of cultivating altogether.
Now with application example the utility model is illustrated:
Respectively with three kinds of different attached cell cell1, cell2, cell3 kind in the bottom of culture dish base and two built-in suspension inner cores, cell attachment and adherent growth, after cultivating one-period, having the built-in suspension inner core of cell cell1 to take out and insert another cultivation cultivation has in the new culture dish of cell4, carries out follow-up cultivation.
Embodiment 2
Respectively three kinds of different attached cell cell1, cell2, cell3 are mixed with hydrogel and plant in culture dish base and two built-in suspension inner cores, cell is grown in the stereoscopic culture environment that hydrogel forms, after cultivating one-period, having the built-in suspension inner core of cell cell1 to take out and insert another cultivation cultivation has in the cultivation inner core of suspension cell cell4, form the mixed culture system that stereoscopic culture and gas-liquid are cultivated mutually, carry out follow-up cultivation.
Claims (6)
1. suspension type cell co-culture device, comprise culture dish base and culture dish lid, described culture dish base has the circular base plane and extends upward the sidewall of formation from the edge, baseplane, it is characterized in that: described culture dish base intracavity is provided with a uncovered suspension at least and cultivates inner core, described suspension is cultivated inner core and is comprised going to the bottom and extend upward the sidewall of being made by the permeable membrane material that forms from the periphery of going to the bottom by the manufacturing of translucidus material, described suspension is cultivated inner core and is provided with suspension holdfast, hangs the cultivation inner core and is fixed in culture dish base inner side-wall by the suspension holdfast suspension.
2. suspension type many cells co-culture device according to claim 1, it is characterized in that: described suspension type cell co-culture device is provided with the suspension of two or more different diameters and cultivates inner core, and the suspension that diameter is little is cultivated inner core and is fixed on the big suspension cultivation inner core inner side-wall of diameter by the suspension holdfast suspension.
3. suspension type many cells co-culture device according to claim 2 is characterized in that: the height that inner core is cultivated in the suspension of described two or more different diameters is superposition successively from the inside to the outside.
4. suspension type many cells co-culture device according to claim 1 and 2, it is characterized in that: described suspension holdfast is made of three suspension arms that are distributed in the upper ledge periphery.
5. suspension type many cells co-culture device according to claim 4, it is characterized in that: described suspension arm is length-adjustable scalable suspension arm.
6. suspension type many cells co-culture device according to claim 1 is characterized in that: described culture dish base and culture dish lid are made by the translucidus material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320040511 CN203048950U (en) | 2013-01-24 | 2013-01-24 | Hanging multicell co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320040511 CN203048950U (en) | 2013-01-24 | 2013-01-24 | Hanging multicell co-culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203048950U true CN203048950U (en) | 2013-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320040511 Expired - Fee Related CN203048950U (en) | 2013-01-24 | 2013-01-24 | Hanging multicell co-culture device |
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| Country | Link |
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| CN (1) | CN203048950U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384719A (en) * | 2018-04-26 | 2018-08-10 | 中国烟草总公司郑州烟草研究院 | Cell co-culture device |
| CN108611273A (en) * | 2018-04-26 | 2018-10-02 | 中国烟草总公司郑州烟草研究院 | Cell co-cultures unit |
| CN110616140A (en) * | 2019-10-28 | 2019-12-27 | 上海国睿生命科技有限公司 | Cell co-culture dish |
| CN117660184A (en) * | 2023-12-11 | 2024-03-08 | 上海生物芯片有限公司 | Separated organ chip model and use method and application thereof |
-
2013
- 2013-01-24 CN CN 201320040511 patent/CN203048950U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384719A (en) * | 2018-04-26 | 2018-08-10 | 中国烟草总公司郑州烟草研究院 | Cell co-culture device |
| CN108611273A (en) * | 2018-04-26 | 2018-10-02 | 中国烟草总公司郑州烟草研究院 | Cell co-cultures unit |
| CN108384719B (en) * | 2018-04-26 | 2023-11-17 | 中国烟草总公司郑州烟草研究院 | cell co-culture device |
| CN108611273B (en) * | 2018-04-26 | 2024-05-03 | 中国烟草总公司郑州烟草研究院 | Cell co-culture unit |
| CN110616140A (en) * | 2019-10-28 | 2019-12-27 | 上海国睿生命科技有限公司 | Cell co-culture dish |
| CN117660184A (en) * | 2023-12-11 | 2024-03-08 | 上海生物芯片有限公司 | Separated organ chip model and use method and application thereof |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130710 Termination date: 20190124 |
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| CF01 | Termination of patent right due to non-payment of annual fee |