CN203048951U - Embedded multicell co-culture device - Google Patents
Embedded multicell co-culture device Download PDFInfo
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- CN203048951U CN203048951U CN 201320040847 CN201320040847U CN203048951U CN 203048951 U CN203048951 U CN 203048951U CN 201320040847 CN201320040847 CN 201320040847 CN 201320040847 U CN201320040847 U CN 201320040847U CN 203048951 U CN203048951 U CN 203048951U
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Abstract
The utility model discloses an embedded multicell co-culture device which comprises a culture dish base, a culture dish cover and a plurality of bottomless frames, wherein the bottomless frames comprise annular upper frames, annular lower frames and annular side walls; the annular side walls are surrounded at the peripheries of the annular upper frames and the annular lower frames and made of permeable membrane materials; a plurality of annular grooves or annular bulges are arranged on the base plane of the culture dish base; and the bottoms of the annular lower frames of the bottomless frames are adaptively connected and fixed with the annular grooves or the annular bulges, so that the inner cavity of a culture dish is divided into a plurality of relatively independent culture chambers. The embedded multicell co-culture device adopts the bottomless frames to divide the whole culture dish into multiple relatively independent culture spaces, realizes exchange of metabolites such as cell factors through the permselectivity of the permeable membrane materials, can realize multiple combination culture of a multicell system and a gas-liquid-solid phase system, and facilitates integrated system research on cell interaction of a complete biological system.
Description
Technical field
The utility model relates to cell culture apparatus, relates in particular to a kind of cellulous co-culture device.
Background technology
Cell cultures is cell to be in grow under the vitro conditions and to breed, and can observe morphological structure of viable cell etc. in culturing process, monitoring and data logging.Cells in vitro is cultivated can adopt single culture mode or various kinds of cell co-cultivation mode.
Cell culture apparatus commonly used is mainly monoclonal gas-liquid phase plane and cultivates, and its unicity and stationarity have limited the multidirectional development of cell cultures, can not satisfy the further investigation to complex systems such as cell interactions.Though also have commercial co-culture device at present, exist to be prone between the different cells and pollute and problem such as mix.Granted publication number is CN202181306, the day for announcing is on April 4th, 2012, denomination of invention discloses a kind of many cells co-culture device for the Chinese utility model patent of " various kinds of cell is cultivated support and various kinds of cell co-culture device altogether ", it is provided with one and cultivates support, this support comprises stent cover and the bracing frame that is connected with stent cover, has fixed orifices on the stent cover.Stent cover edge is connected with bracing frame.Cultivate support and put into culture dish, the fixed orifices of cultivating support is used for hanging cultivates cell, and each cultivates the cultivation that cell is used for a kind of cell.Then this culture dish, cultivate support and suspension type altogether and cultivate the culture system that cell becomes a various kinds of cell co-cultivation.Various cells grow in respectively in different the cultivation cell or culture dish.Be provided with the aperture that varies in size in the bottom of cultivating cell, can allow liquid freely to pass through, and cell can not pass through.This co-culture device is suitable for the common training method of some cell.And, be difficult to protect for the size setting in aperture and hold the cultivation of inapplicable minicell.
The utility model content
The purpose of this utility model is to remedy the deficiencies in the prior art, the many cells co-culture device of provide a kind of function practicality, strong operability, can realize many cells system, the Multiple Combination of the solid heterogeneous system of gas-liquid being cultivated.
To achieve these goals, the utility model has adopted following technical scheme: a kind of embedded many cells co-culture device, comprise culture dish base and culture dish lid, also comprise some no bottom frameworks, described no bottom framework comprises annular upper ledge, annular lower frame and around the annular sidewall that is constituted by the permeable membrane material of annular upper ledge and annular lower frame periphery, the baseplane of described culture dish base is provided with some annular recessess or annular protrusion, frame bottom and described annular recesses or annular protrusion are connected and fixed suitablely under the annular of described no bottom framework, thereby described culture dish inner chamber is separated into several relatively independent cultivation cells.The mutual intussusception of no bottom framework of different diameter forms the cultivation cell that comprises mutually.
As a kind of embodiment, the baseplane of described culture dish base is provided with annular recesses or the annular protrusion of the above concentric structure of two circles, the no bottom framework of plural different diameter and described annular recesses or suitable being connected and fixed of annular protrusion.
As another kind of embodiment, the baseplane of described culture dish base is provided with annular recesses or the annular protrusion of the mutually noninterfere more than two, plural equal diameters or the no bottom framework that does not wait and described annular recesses or suitable being connected and fixed of annular protrusion.Each no bottom framework all limits one and independently cultivates cell.
The utility model can be divided into a plurality of relatively independent culture space with whole culture dish by arranging of no bottom framework, and realizes the exchange of metabolism products such as cytokine by the selection perviousness of permeable membrane material.The utility model is at systematize and the precision of cells in vitro culture system, set up the common cultivation formula cell culture apparatus of novel practical, the function practical simplicity, workable, can realize the Multiple Combination cultivation of many cells system, the solid heterogeneous system of gas-liquid, more the external many cells of real simulated interact and amplification procedure, are convenient to total system research is carried out in the cell interaction of complete living things system.
Description of drawings
Fig. 1 is the utility model one-piece construction synoptic diagram.
Fig. 2 is the decomposing schematic representation of culture dish base.
Fig. 3 is the bracing frame synoptic diagram of framework.
Fig. 4 is the culture dish base vertical view of another embodiment.
Wherein, encircle 1.22 permeable membranes, 1.23 culture dish lid 2.00 under ring 1.21 second frameworks on ring 1.12 permeable membranes 1.13 second frameworks under ring 1.11 first frameworks on culture dish base 1.00 grooves 1.01 first frameworks
Embodiment
As shown in Figures 1 and 2, embedded many cells co-culture device of the present utility model comprises culture dish base 1.00, culture dish lid 2.00 and is arranged at the no bottom framework of culture dish base 1.00 inner chambers.Culture dish base 1.00 has a circular base plane and extends upward the sidewall of formation from the edge, baseplane, the circular base plane is provided with the groove 1.01 of some annulars, groove 1.01 is used for fixed support on the one hand, also the baseplane of culture dish base is divided into several zones on the other hand.The inner chamber of culture dish base is provided with several no bottom frameworks, considers from practical application, is advisable with annular frame, and quantity can need to decide on experiment for more than 2 or 2.In this example, be provided with first framework and second framework of different diameter, second framework of path is positioned at first frame center in big footpath, and the diameter of first framework and second framework respectively with culture dish base baseplane two the circle grooves 1.01 diameter corresponding.Preferably, first framework and second framework are sheathed in concentrically ringed mode.Second frame height that is positioned at inner ring is lower than the height of first framework that is positioned at the outer ring.First framework is by encircling under 1.11, first framework ring 1.12 on first framework and constituting around the permeable membrane 1.13 of upper and lower ring periphery.Between the upper and lower ring many support bars can be set, as shown in Figure 3.In like manner, second framework is by encircling under 1.21, second framework ring 1.22 on second framework and constituting around the permeable membrane 1.23 of upper and lower ring periphery.Following ring is embedded in the groove 1.01, thereby makes first framework and second frame fixation in the culture dish base, and the culture dish base intracavity is separated into three mutual sheathed cultivation cells.
As another kind of embodiment, the culture dish base intracavity arranges the no bottom framework of a plurality of mutually noninterferes, is evenly distributed in culture dish base baseplane, as shown in Figure 4.These no bottom frameworks can be isometrical also can be waney, need decide on experiment.
The form of annular projection also can be adopted except the form of groove is set in the baseplane of culture dish base, and correspondingly, no bottom framework bottom adopts sunk structure to match with this annular projection.
Culture dish 1.00 and culture dish lid 2.00 can adopt existing standard culture device, adopt translucidus cell culture material commonly used to make.No bottom framework can be selected to see through different cytokine and meta-bolitess according to different permeable membrane materials, can change different permeable membrane supports as required at any time in whole cell cultivation process, to change kind and the concentration of cytokine in the nutrient solution.
Now with application example the utility model is illustrated:
The one the second no bottom frameworks are fixed in the culture dish base, form three sheathed culture space successively, plant respectively three kinds of different adherent or suspension cell cell1, cell2, cell3 between three annular zonees in, cell is at adherent or suspension growth in the space independently separately, the cytokine that exists the different sorts cell to produce in the nutrient solution promotes the growth of cell in the many cells complex system by various factor interactions.
Embodiment 2
The one the second no bottom frameworks are fixed in the culture dish base, form three relatively independent culture space, plant attached cell cell1, cell2, cell3 respectively between three annular zonees.Wherein, the permeable membrane of no bottom framework employing aperture is the hydrophilic perviousness mould material of n1.After cultivating one-period, being replaced by the aperture is the lipophilic perviousness mould material support of n2, with the kind of the cytokine that changes okioplast and internal layer cell interaction, to carry out follow-up cultivation.
Embodiment 3
The one the second no bottom frameworks are fixed in the culture dish base, form three relatively independent culture space, plant respectively three kinds of different adherent or suspension cell cell1, cell2, cell3 between three annular zonees in, cell is at adherent or suspension growth in the space independently separately.After cultivating one-period, take off the second no bottom framework that is positioned at internal layer, the ectonexine cell is in contact with one another and the expansion growth space, and it is long to form the contact symbiosis, to carry out follow-up cultivation.
Claims (3)
1. embedded many cells co-culture device, comprise culture dish base and culture dish lid, it is characterized in that: also comprise some no bottom frameworks, described no bottom framework comprises annular upper ledge, annular lower frame and around the annular sidewall that is constituted by the permeable membrane material of annular upper ledge and annular lower frame periphery, the baseplane of described culture dish base is provided with some annular recessess or annular protrusion, frame bottom and described annular recesses or annular protrusion are connected and fixed suitablely under the annular of described no bottom framework, thereby described culture dish inner chamber is separated into several relatively independent cultivation cells.
2. embedded many cells co-culture device according to claim 1, it is characterized in that the baseplane of described culture dish base is provided with annular recesses or the annular protrusion of the above concentric structure of two circles, the no bottom framework of plural different diameter and described annular recesses or suitable being connected and fixed of annular protrusion.
3. embedded many cells co-culture device according to claim 1, it is characterized in that: the baseplane of described culture dish base is provided with annular recesses or the annular protrusion of the mutually noninterfere more than two, plural equal diameters or the no bottom framework that does not wait and described annular recesses or suitable being connected and fixed of annular protrusion.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201320040847 CN203048951U (en) | 2013-01-24 | 2013-01-24 | Embedded multicell co-culture device |
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| CN 201320040847 CN203048951U (en) | 2013-01-24 | 2013-01-24 | Embedded multicell co-culture device |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103460993A (en) * | 2013-08-27 | 2013-12-25 | 辽宁省微生物科学研究院 | Macro fungus demethylation breading method and macro fungus demethylation breading device |
| WO2015013817A1 (en) * | 2013-07-29 | 2015-02-05 | Uti Limited Partnership | Annular culture dish |
| US20150252329A1 (en) * | 2012-10-09 | 2015-09-10 | The University Of North Carolina At Chapel Hill | Methods and apparatus for culturing ciliated cells |
| CN107058098A (en) * | 2017-03-01 | 2017-08-18 | 北京科技大学 | A kind of device and method of the cell patterning culture based on 3D printing technique |
| USD825076S1 (en) | 2016-11-23 | 2018-08-07 | Clifford L. Librach | Tissue culture dish |
| CN108611273A (en) * | 2018-04-26 | 2018-10-02 | 中国烟草总公司郑州烟草研究院 | Cell co-cultures unit |
| CN109679833A (en) * | 2019-01-10 | 2019-04-26 | 航天神舟生物科技集团有限公司 | Multifactor integration screening flat board and its tools and production method |
| CN109735428A (en) * | 2019-01-10 | 2019-05-10 | 航天神舟生物科技集团有限公司 | Device and method for microbial variation under space environment and derivation research |
| CN111543328A (en) * | 2020-06-19 | 2020-08-18 | 福建省中科生物股份有限公司 | Gold thread lotus bud seedling liquid tissue culture apparatus |
| CN112041422A (en) * | 2018-02-08 | 2020-12-04 | 佛罗里达大学研究基金会公司 | Bioreactor with perfusion function |
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2013
- 2013-01-24 CN CN 201320040847 patent/CN203048951U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150252329A1 (en) * | 2012-10-09 | 2015-09-10 | The University Of North Carolina At Chapel Hill | Methods and apparatus for culturing ciliated cells |
| WO2015013817A1 (en) * | 2013-07-29 | 2015-02-05 | Uti Limited Partnership | Annular culture dish |
| CN103460993A (en) * | 2013-08-27 | 2013-12-25 | 辽宁省微生物科学研究院 | Macro fungus demethylation breading method and macro fungus demethylation breading device |
| USD825076S1 (en) | 2016-11-23 | 2018-08-07 | Clifford L. Librach | Tissue culture dish |
| CN107058098A (en) * | 2017-03-01 | 2017-08-18 | 北京科技大学 | A kind of device and method of the cell patterning culture based on 3D printing technique |
| CN112041422A (en) * | 2018-02-08 | 2020-12-04 | 佛罗里达大学研究基金会公司 | Bioreactor with perfusion function |
| CN108611273A (en) * | 2018-04-26 | 2018-10-02 | 中国烟草总公司郑州烟草研究院 | Cell co-cultures unit |
| CN108611273B (en) * | 2018-04-26 | 2024-05-03 | 中国烟草总公司郑州烟草研究院 | Cell co-culture unit |
| CN109679833A (en) * | 2019-01-10 | 2019-04-26 | 航天神舟生物科技集团有限公司 | Multifactor integration screening flat board and its tools and production method |
| CN109735428A (en) * | 2019-01-10 | 2019-05-10 | 航天神舟生物科技集团有限公司 | Device and method for microbial variation under space environment and derivation research |
| CN111543328A (en) * | 2020-06-19 | 2020-08-18 | 福建省中科生物股份有限公司 | Gold thread lotus bud seedling liquid tissue culture apparatus |
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| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130710 Termination date: 20190124 |