CN204434602U - A kind of planktonic algae cell microwell array culture apparatus - Google Patents
A kind of planktonic algae cell microwell array culture apparatus Download PDFInfo
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- CN204434602U CN204434602U CN201520045247.5U CN201520045247U CN204434602U CN 204434602 U CN204434602 U CN 204434602U CN 201520045247 U CN201520045247 U CN 201520045247U CN 204434602 U CN204434602 U CN 204434602U
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- planktonic algae
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Abstract
The utility model discloses a kind of planktonic algae cell culture apparatus, comprise base plate, base plate is provided with microwell array, described microwell array is by the orthogonal array of pore arrangement, spacing 3mm between adjacent cells array ~ 10mm, described micropore size is of a size of 10 μm ~ 30 μm, and micropore depth size is 20 μm ~ 30 μm, and the spacing between adjacent cells is in 2 μm ~ 3 μm.Microwell array on base plate, is a large amount of micropores being etched composition by specific microscale complete processing (as laser etching techniques) on base plate, and constitutes specific array.By device of the present utility model can by mutually isolated for different planktonic algae cell, do not interfere with each other, algal grown Habitat requirement (illumination, nutritive salt etc.) can be provided, the cell cultures of planktonic algae being carried out on micro-scale can be met.
Description
Technical field
The utility model patent relates to a kind of plane space and forms necessarily regularly arranged microwell array, for planktonic algae cell cultures, particularly relates to a kind of planktonic algae cell microwell array culture apparatus based on microscale processing.
Background technology
Algae is the holophytes such as a kind of unicellular or many cells are low, containing photosynthetic pigments, does not have real root, stem, leaf to break up, carries out vegetative propagation mainly through spore or zygote.Wherein, there is most algae suspension growth suitable in water body, be referred to as planktonic algae (or " plant plankton "), they are usually individual tiny, cell dia is within the scope of several microns to some tens of pm, naked eyes are not easily shown in, but they contribute to the primary productivity of global aquatic ecosystem (ocean, land) more than 90%, are that food chain is formed and the most important organism of ecosystem development.
But the planktonic algae under field conditions (factors), in water body presents the state of mixed growth.Mainly present following 2 features: under the site conditions that 1) the planktonic algae mixed growth of different population is identical, even if thousands of up to a hundred different algal species also may be had in a water; 2) planktonic algae being in different growth conditions (resting stage, exponential phase of growth, logarithmic phase, endogenous respiration phase, dead residual body etc.) also mixed growth under identical site conditions, even if the same algae kind being in difference " age " section also may be found in a water.
Be subject to the restriction of traditional macro research means, planktonic algae physiological ecological research for a long time all concentrates on macro-scale, namely by obtaining the planktonic algae (population or mixed population) compared with mcroorganism amount to algae kind single after separation and purification (or multiple algae kind) enlarged culturing (as beaker, flask, other reactor or insitu soil tests etc.), design different tests operating mode on this basis and conduct a research.But due to the existence of above-mentioned mixed growth feature, planktonic algae iuntercellular may to exist between cut-throat competition, each cell secreta also to exist to site conditions such as illumination, nutritive salt, spaces and influences each other, therefore the research technique of traditional macro yardstick has no idea to solve the problem of biologically " population effect ".Therefore carry out the research of microhabitat to aspects such as the interaction relationships between planktonic algae cell physiological ecological impact, different planktonic algae cell, still lack effective test method.
Utility model content
In view of above-mentioned, the utility model provides a kind of planktonic algae cell culture apparatus based on microscale processing.By microscale complete processing, can by mutually isolated for different planktonic algae cell, good biocompatibility, the culture apparatus that can provide algal grown Habitat requirement (illumination, nutritive salt etc.), to meet the cell cultures of to carry out planktonic algae on micro-scale.
For achieving the above object and other relevant objects, the utility model provides a kind of planktonic algae cell culture apparatus, comprise base plate, base plate is provided with microwell array, spacing 3mm between adjacent cells array ~ 10mm, described microwell array is by the orthogonal array of pore arrangement, and described micropore size is of a size of 10 μm ~ 30 μm, micropore depth size is 20 μm ~ 30 μm, and the spacing between adjacent cells is in 2 μm ~ 3 μm.Microwell array, is positioned on base plate, is a large amount of micropores being etched composition by specific microscale complete processing (as laser etching techniques) on base plate, and constitutes specific array.
As preferably, described base plate is made up of silicon or silicon-dioxide or polydimethylsiloxane.Base plate adopt biocompatibility better, water white transparency, there is some strength, and the material composition of microscale processing can be carried out.
As preferably, described base plate is provided with 9 ~ 16 microwell arrays.
As preferably, the micropore quantity of described microwell array is 100 ~ 10000, namely 10 × 10 ~ 100 × 100.
As preferably, described micropore is cylinder hole, and cross-sectional shape is circular or square or equilateral hexagon.
According to culture apparatus of the present utility model, for ease of alga cells counting, the micropore on array is by matrix-style assortment, and the micropore quantity on single array is 100 × 100.On microwell array, monolateral row, column is all numbered by Arabic numerals.For convenience of counting, the Separation control between microwell array is at more than 3mm.For meeting operability, the microwell array on a base plate controls between 9 (3 × 3) to 16 (4 × 4).
Further, described micropore, cross-sectional shape is circular, square, equilateral hexagon and so on, the size shape of the opening of micropore and the size shape of microvia bottom similar, according to planktonic algae cell size, pore size (bore, the degree of depth) answers 2 ~ 3 times to the size range of testing sample planktonic algae cell, ensure at least 1 planktonic algae cell, be no more than 5 planktonic algae cells at the most and can enter in micropore and cultivate, and ensure that planktonic algae cell can grow and not run off because nutrient solution injection in micropore.According to the size range of current most of planktonic algae cell, the size range of recommending aperture is 10 μm ~ 30 μm, and micropore hole depth should control more than 20 μm, and the hole depth scope usually controlled is 20 μm ~ 30 μm.
Further, for promoting that planktonic algae cell is inoculated smoothly into micropore, on the base plate avoiding planktonic algae cell to rest in seeded process between micropore and micropore, and ensure to have enough strengths of materials between micropore and micropore, the spacing between different micropore controls at 2 μm ~ 3 μm simultaneously.
Compared to existing technology, the utility model has following beneficial effect:
Cultivate between micropore and be spaced from each other, do not interfere with each other between the frustule in each micropore, the upgrowth situation of frustule under Individual existence condition can be fully demonstrated.
Effectively determine the spacing between different micropore in microwell array, can promote that planktonic algae cell is inoculated smoothly into micropore, avoid planktonic algae cell in seeded process, rest on base plate between micropore and micropore.
Very little owing to cultivating micropore, be arranged in array between micropore, therefore can save lab space, much can organize parallel laboratory test in very little spatially doing.
Cultivating micropore is array format ordered arrangement, can realize the orderly cultivation of planktonic algae cell, realize the planktonic algae cell culture condition of homogenization.
The volume in hole own is little, area is few, and in culturing process, required algae kind is few, thus can carry out on a large scale testing and being unlikely to cause difficulty to indoor algal species cultivation work.
Accompanying drawing explanation
Fig. 1 is the microwell array distribution schematic diagram of the culture apparatus of embodiment of the present utility model.
Fig. 2 is part microwell array enlarged view in Fig. 1.
Fig. 3 is the sectional view of the microwell array of Fig. 2.
Fig. 4 be adopt the present embodiment cultivation microwell plate on the number change figure of chlorella in a certain little microwell array.
Piece mark explanation
1-base plate,
2-microwell array,
3-micropore.
Embodiment
By particular specific embodiment, embodiment of the present utility model is described below, person skilled in the art scholar the content disclosed by this specification sheets can understand other advantages of the present utility model and effect easily.
As shown in Figure 1, Figure 2 and Figure 3, the utility model frustule is cultivated microwell array algae and is comprised PDMS base plate 1, PDMS base plate 1 is carved with 9 microwell arrays 2, microwell array 2 is the matrix distribution of 3 × 3, the spacing of adjacent cells array 2 is 4mm, and each microwell array 2 is formed by 100 × 100 micropores 3, and arranges orthogonal, micropore 3 is cylindrical micro-culture hole, and the diameter of micropore 3 and hole depth are respectively 20 μm.
Testing of characteristic is frustule growth experiment, concrete operations and result as follows:
Draw the certain density chlorella algae liquid of 10 μ L on microwell array culture plate, covered gently, makes algae liquid be dispersed in monoblock well plates along cover glass; Static 10min, light microscopic counting cultivates the frustule number in micropore; Well plates after counting is placed in substratum together with cover glass cultivate; The change of cell count in every day time recording micropore, continuous recording 19 days.
Can find out that chlorella grows fine in PDMS micropore by the growth curve shown in Fig. 4, this micropore culture apparatus is suitable for the cultivation of frustule.
Above-described embodiment is illustrative principle of the present utility model and effect thereof only, but not for limiting the utility model.Any person skilled in the art scholar all without prejudice under spirit of the present utility model and category, can modify above-described embodiment or changes.Therefore, such as have in art and usually know that the knowledgeable modifies or changes not departing from all equivalences completed under the spirit and technological thought that the utility model discloses, must be contained by claim of the present utility model.
Claims (5)
1. a planktonic algae cell culture apparatus, it is characterized in that, comprise base plate, base plate is provided with microwell array, spacing 3mm between adjacent cells array ~ 10mm, described microwell array is by the orthogonal array of pore arrangement, and described micropore size is of a size of 10 μm ~ 30 μm, micropore depth size is 20 μm ~ 30 μm, and the spacing between adjacent cells is in 2 μm ~ 3 μm.
2. planktonic algae cell culture apparatus according to claim 1, is characterized in that, described base plate is made up of silicon or silicon-dioxide or polydimethylsiloxane.
3. planktonic algae cell culture apparatus according to claim 2, is characterized in that, described base plate is provided with 9 ~ 16 microwell arrays.
4. planktonic algae cell culture apparatus according to claim 3, is characterized in that, the micropore quantity of described microwell array is 100 ~ 10000.
5. planktonic algae cell culture apparatus according to claim 4, is characterized in that, described micropore is cylinder hole, and cross-sectional shape is circular or square or equilateral hexagon.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520045247.5U CN204434602U (en) | 2015-01-23 | 2015-01-23 | A kind of planktonic algae cell microwell array culture apparatus |
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| CN201520045247.5U CN204434602U (en) | 2015-01-23 | 2015-01-23 | A kind of planktonic algae cell microwell array culture apparatus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106006545A (en) * | 2016-05-27 | 2016-10-12 | 广东工业大学 | Conical micro-pore array and preparation method thereof |
| CN106119095A (en) * | 2016-06-30 | 2016-11-16 | 中国科学院重庆绿色智能技术研究院 | A kind of film substrate micro through hole array biochip and preparation method thereof |
| CN110106064A (en) * | 2019-06-03 | 2019-08-09 | 中国矿业大学 | A kind of microalgae fast screening devices and microalgae rapid screening method |
-
2015
- 2015-01-23 CN CN201520045247.5U patent/CN204434602U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106006545A (en) * | 2016-05-27 | 2016-10-12 | 广东工业大学 | Conical micro-pore array and preparation method thereof |
| CN106119095A (en) * | 2016-06-30 | 2016-11-16 | 中国科学院重庆绿色智能技术研究院 | A kind of film substrate micro through hole array biochip and preparation method thereof |
| CN110106064A (en) * | 2019-06-03 | 2019-08-09 | 中国矿业大学 | A kind of microalgae fast screening devices and microalgae rapid screening method |
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