CN201695031U

CN201695031U - Partitioned culture vessel with glass bottom

Info

Publication number: CN201695031U
Application number: CN 201020196538
Authority: CN
Inventors: 周密
Original assignee: Fudan University
Current assignee: Fudan University
Priority date: 2010-05-17
Filing date: 2010-05-17
Publication date: 2011-01-05
Anticipated expiration: 2020-05-17

Abstract

The utility model belongs to the technical field of microorganism cultural appliances, and relates to a partitioned culture vessel with a glass bottom. The partitioned culture vessel with a glass bottom comprises a vessel base side wall, partition walls, a bottom wall hollow-out portion of a vessel base, cover glass piece, a wall of the vessel base and a side wall and a top wall of a vessel top cover. The partitioned culture vessel adopts non-toxic polyvinyl chloride plates which are manufactured into the circular vessel base and the vessel top cover in a plastic suction or pressing form, the convex partition walls are arranged in the vessel base to evenly divide the culture vessel into a plurality of small chambers, and the center of the bottom wall of the vessel base is hollow. By aid of the partitioned culture vessel, people can simultaneously observe various cells on an inverted fluorescence microscope, and can simultaneously shoot various or multiple groups of cells on a living cell workstation loaded with an XY platform. In addition, the partitioned culture vessel is simple in preparation, convenient in use and low in price.

Description

Culture dish at the bottom of the lattice glass

Technical field

The utility model belongs to microorganism culturing technique with the apparatus field, is specifically related to culture dish at the bottom of a kind of lattice glass.

Background technology

At present, in research population and healthy life science, the growth and the change procedure that continue of cell more and more paid close attention in beginning, and this just need utilize the viable cell workstation to come that viable cell is grown time-histories and observe.Domestic experimenter is the most normal, and what select for use is that small enclosed is cultivated cell.It is installed in the inside that is used for accurately controlling the localized piezoelectric ceramic device of Z axle on the inverted fluorescence microscope Stage microscope usually.Because the size of piezoelectric ceramic device has limited the size of cultivating cell, can only place next 35mm glass floor cells culture dish in the domestic modal cultivation cell usually, only can satisfy a kind of cell of experimental observation one time.But in the experiment practice, be starved of the growth change process of observing two kinds of (even multiple) cells simultaneously, two remarkable advantages have been done like this, the one, the variation of compare combination experiment group better, for example: in certain cell cycle, under the same environment, what similarities and differences whether the reaction of observing the cell of or not of dosing have; The 2nd, improve conventional efficient, observe several samples simultaneously, carry out many group experiments.And the viable cell workstation that has disposed the XY platform at present can carry out multi-point scanning fully simultaneously, promptly can observe a plurality of zones at the bottom of the ware simultaneously, and this also becomes to provide technical guarantee for observing various kinds of cell simultaneously.

At present, simultaneously various kinds of cell is carried out on the viable cell workstation unique problem that continuous observation post runs into be exactly the disposable 35mm glass of using always at the bottom of culture dish can only place a kind of cell.Though existing in the world 35mm separates culture apparatus more, but rarely have domestic experimenter to use.Reason has two: the disposable use of right and wrong, and the palpus disinfection has increased experimental procedure before using; Moreover be exactly that its price is very expensive; In addition, at the bottom of external existing many lattices ware is not glass, so can't be used for observing on the inverted fluorescence microscope.

Summary of the invention

The purpose of this utility model is for overcoming the defective of prior art, and culture dish at the bottom of a kind of lattice glass is provided.Be separated at the bottom of many lattices glass at the bottom of this culture dish ware, this culture dish can be applied in the supporting small enclosed of domestic the most frequently used and viable cell preferably and cultivate in the cell, overcomes glass floor cells culture dish and can only place the inconvenience experimentally that a kind of cell causes.

Particularly, culture dish at the bottom of a kind of lattice glass of the present utility model, it is characterized in that it comprises diapire hollow out (3), cover glass (4), the wall (5) of ware base, the sidewall (6) of ware top cover and the roof (7) of ware top cover of ware base side wall (1), partition wall (2), ware base.

Culture dish adopts non-toxic polyvinyl chloride sheet material at the bottom of the utility model lattice glass, through plastic uptake or be pressed into circular ware base and ware top cover, the ware top cover comprises sidewall (6) and roof (7), comprise base side wall (1) and ware base bottom wall (5) at the bottom of the ware, be provided with the partition wall (2) of protrusion in the ware base, the ware base is divided into a plurality of cells, preferably establish four cells in the utility model, the diapire center of ware base is hollow out (3), the separation of middle openwork part protrusion downwards is concordant with the lower edge of diapire (5) outer peripheral portion of ware base, during use, can adopt medical grade do not have shadow glue with cover glass stick at the bottom of the ware hollow out position and around diapire, guarantee at the bottom of the ware and the mutual seepage of liquid that can not between separating.

In the utility model, circular ware base and its diameter of ware top cover are 35mm.

In the utility model, the diapire hollow out diameter of described ware base is 10-20mm.

In the utility model, the thickness of described cover glass is 0.13-0.17mm.

Use through test, this culture dish can be applied in the supporting small enclosed of domestic the most frequently used and viable cell preferably and cultivate in the cell, overcome glass floor cells culture dish and can only place the inconvenience experimentally that a kind of cell causes, the utility model preparation is simple, easy to use, and price is low, provides good experimental tool at the viable cell workstation various kinds of cell being carried out continuous observational study at present.

The utlity model has following advantage:

1, can on inverted fluorescence microscope, observe various kinds of cell simultaneously;

2, on the viable cell workstation of prestowage XY platform, take multiple simultaneously or organize cell more.

For the ease of understanding, below will by concrete drawings and Examples to lattice glass of the present utility model at the bottom of culture dish describe in detail.It needs to be noted, specific examples and accompanying drawing only are in order to illustrate, obviously those of ordinary skill in the art can illustrate according to this paper, in scope of the present utility model the utility model is made various corrections and change, these corrections and change are also included in the scope of the present utility model.

Description of drawings

Fig. 1 is a culture dish base plan structure synoptic diagram at the bottom of the lattice glass.

Fig. 2 is a culture dish top cover plan structure synoptic diagram at the bottom of the lattice glass.

Fig. 3 is a culture dish base sectional structure synoptic diagram at the bottom of the lattice glass.

Fig. 4 is a culture dish top cover sectional structure synoptic diagram at the bottom of the lattice glass.

Fig. 5 is a culture dish surface structure synoptic diagram at the bottom of the lattice glass.

Be denoted as among the figure: the 1st, ware base side wall, the 2nd, partition wall, the 3rd, the diapire hollow out, the 4th of ware base, cover glass, the 5th, the wall of ware base, the 6th, the sidewall of ware top cover, the 7th, the roof of ware top cover.

Embodiment

Embodiment 1

The structure of culture dish is shown in Fig. 1-5 at the bottom of the exemplary lattice glass, and it comprises diapire hollow out (3), cover glass (4), the wall (5) of ware base, the sidewall (6) of ware top cover and the roof (7) of ware top cover of ware base side wall (1), partition wall (2), ware base.Adopt non-toxic polyvinyl chloride sheet material, through plastic uptake or be pressed into circular ware base and the ware top cover of diameter 35mm, the ware top cover comprises base side wall (1) and ware base bottom wall (5) by sidewall (6) and roof (7) formation at the bottom of the ware; Be provided with the partition wall (2) of protrusion in the ware base, four cells will be divided in the ware base, the diapire center of ware base is hollow out (3), the hollow out diameter is 10-20mm, the separation of middle openwork part protrusion downwards is concordant with the lower edge of diapire (5) outer peripheral portion of ware base, during use, can adopt medical grade do not have shadow glue with the thick cover glass of 0.13-0.17mm stick at the bottom of the ware hollow out position and around diapire, guarantee at the bottom of the ware and the mutual seepage of liquid that can not between separating.

Claims

1. culture dish at the bottom of the lattice glass is characterized in that, it comprises diapire hollow out (3), cover glass (4), the wall (5) of ware base, the sidewall (6) of ware top cover and the roof (7) of ware top cover of ware base side wall (1), partition wall (2), ware base.

2. by culture dish at the bottom of the described lattice glass of claim 1, it is characterized in that the ware base of culture dish and ware top cover are circular at the bottom of the described lattice glass, ware base or ware top cover diameter are 35mm.

3. by culture dish at the bottom of the described lattice glass of claim 1, it is characterized in that, be provided with the partition wall (2) of protrusion in the described ware base.

4. by culture dish at the bottom of the described lattice glass of claim 1, it is characterized in that described ware base is divided into a plurality of cells.

5. by culture dish at the bottom of claim 1 or the 4 described lattice glass, it is characterized in that described ware base is divided into and is divided into four cells.

6. by culture dish at the bottom of the described lattice glass of claim 1, it is characterized in that the diapire center of described ware base is hollow out (3), the separation of hollow out (3) protrusion downwards is concordant with the lower edge of diapire (5) outer peripheral portion of ware base.

7. by culture dish at the bottom of the described lattice glass of claim 5, it is characterized in that the hollow out diameter of the diapire of described ware base (5) is 10-20mm.

8. by culture dish at the bottom of the described lattice glass of claim 1, it is characterized in that the thickness of described cover glass (4) is 0.13-0.17mm.