CN205347445U - Cell culture fibre membrane support frame, cell culture support and cell culture device - Google Patents
Cell culture fibre membrane support frame, cell culture support and cell culture device Download PDFInfo
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- CN205347445U CN205347445U CN201620066733.XU CN201620066733U CN205347445U CN 205347445 U CN205347445 U CN 205347445U CN 201620066733 U CN201620066733 U CN 201620066733U CN 205347445 U CN205347445 U CN 205347445U
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- 239000012528 membrane Substances 0.000 title claims abstract description 126
- 238000004113 cell culture Methods 0.000 title claims abstract description 57
- 239000000835 fiber Substances 0.000 title abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 8
- 238000012258 culturing Methods 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 18
- 238000010041 electrostatic spinning Methods 0.000 claims description 10
- 239000004626 polylactic acid Substances 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 6
- 239000008188 pellet Substances 0.000 claims description 6
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 6
- 229920001436 collagen Polymers 0.000 claims description 5
- 238000000465 moulding Methods 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 229920001610 polycaprolactone Polymers 0.000 claims 1
- 239000004632 polycaprolactone Substances 0.000 claims 1
- 238000011081 inoculation Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 161
- 239000000243 solution Substances 0.000 description 18
- 238000002474 experimental method Methods 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 8
- 230000010261 cell growth Effects 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 6
- 239000005030 aluminium foil Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000010276 construction Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 210000001822 immobilized cell Anatomy 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 230000010412 perfusion Effects 0.000 description 2
- 238000010146 3D printing Methods 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000011796 hollow space material Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000002121 nanofiber Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture fibre membrane support frame, cell culture support and cell culture device relates to medical consumptive material technical field. For solving prior art inoculation inefficiency when adopting the cell culture fibre membrane to carry out cell culture, cultivate and detect the big problem of the degree of difficulty and invent. The utility model discloses cell culture fibre membrane support frame is used for supporting the cell culture fibre membrane, the cell culture fibre membrane is used for cell culture, the support frame is formed with and supports the mouth, support mouthful with cell culture fibre membrane phase -match, it is used for supporting fixedly to support the mouth border a week of cell culture fibre membrane. The utility model discloses cell culture fibre membrane support frame, cell culture support and cell culture device can be used to improve cell inoculation efficiency and reduces cell culture and detect the degree of difficulty.
Description
Technical field
This utility model relates to medical treatment consumptive materials technical field, particularly relates to a kind of cell and cultivates fibrous membrane bracing frame, cell culturing bracket and cell culture system.
Background technology
Electrostatic spinning is that one utilizes polymer solution or melt to form the injection stream ultra-fine long stapled novel nano fiber technology of preparing of acquisition under highfield effect, the cell prepared by this technology is utilized to cultivate the three-dimensional network-like structure that fibrous membrane is intertwined to form for elongated fibers, there is higher specific surface area and porosity, natural extra-cellular matrix structure can be simulated well, growth and propagation for cell provide good microenvironment, therefore generally adopt this cell to cultivate fibrous membrane at biological and medical field and carry out cell cultivation.
When adopting this cell cultivation fibrous membrane to carry out cell cultivation, fibrous membrane can be carried out cutting according to culture vessel bottom shape size and repave in culture vessel, and adopt common law that cell is seeded to cell to cultivate on fibrous membrane and cultivate, this commonsense method is: is directly covered with to bottom in the culture vessel of cell cultivation fibrous membrane and adds cell solution (solution that namely cell and culture medium are made into), and the cell in cell solution sinks to being seeded to cell and cultivates on fibrous membrane.The method is simple to operate, inoculation efficiency is higher, and cell is usually uniformly distributed in cell and cultivates on fibrous membrane, each cell all can absorb nutrient preferably, is conducive to the growth of cell, but, owing to this cell cultivation fibrous membrane is relatively thin, lighter weight, therefore add in culture vessel in cell solution process, cell is cultivated fibrous membrane and is often produced to suspend and fold, makes follow-up cell cultivate and detection difficulty increases.
In order to avoid the problems referred to above, cell can be cultivated fibrous membrane and be fixed on bottom of culture vessel or slide, different size slide is applicable to the culture vessel of different size, slide be smaller in size than bottom of culture vessel size in case slide is put into adaptation bottom of culture vessel, in order to cell is all inoculated on fibrous membrane, dibbling method can be adopted to be seeded to by cell on cell cultivation fibrous membrane cultivate, this dibbling method is: first make the cell solution of high concentration, then the cell solution of this high concentration is uniformly dropped to the cell being layed on bottom of culture vessel or slide and cultivates on fibrous membrane, after culture vessel is put in incubator certain time, it is supplemented with culture medium.Cultivate fibrous membrane due to cell and be attached on bottom or the slide of culture vessel all the time, thus without producing suspension fold.But, compare common law, cell solution need to uniformly be dropped to cell and cultivate the diverse location on fibrous membrane by dibbling process, this seeded process is consuming time longer, inefficient, and it is excessive to easily cause local location cell yield, internal cell is easily die less than nutrition because of absorption, and it is bigger that cell cultivates difficulty.Simultaneously as local location increment is excessive, cell cultivates the skewness on fibrous membrane at cell, therefore the cell detection results in later stage is had considerable influence.
Utility model content
Embodiment of the present utility model provides a kind of cell to cultivate fibrous membrane bracing frame, cell culturing bracket and cell culture system, it is prevented from cell and cultivates fibrous membrane shrinkage, common law can be adopted simultaneously to cultivate cell, thus improving cell inoculation efficiency, reducing cell and cultivating and detection difficulty.
For reaching above-mentioned purpose, embodiment of the present utility model provides a kind of cell and cultivates fibrous membrane bracing frame, fibrous membrane is cultivated for supporting cell, described cell is cultivated fibrous membrane and is cultivated for cell, support frame as described above is formed with supporting mouth, described supporting mouth and described cell are cultivated fibrous membrane and are matched, and described supporting mouth cultivates the edge one week of fibrous membrane for supporting fixing described cell.
Embodiment of the present utility model additionally provides a kind of cell culturing bracket, including: cell cultivates fibrous membrane, and described cell is cultivated fibrous membrane and is used for carrying cell；Bracing frame, support frame as described above is as above bracing frame described in technical scheme, and described cell is cultivated the edge of fibrous membrane and within one week, is fixedly connected on the supporting mouth place of support frame as described above.
This utility model embodiment additionally provides a kind of cell culture system, including: cell culturing bracket, described cell culturing bracket is as above cell culturing bracket described in technical scheme, and in described cell culturing bracket, the supporting mouth of bracing frame stretches in described culture vessel and arranges.
A kind of cell that this utility model embodiment provides cultivates fibrous membrane bracing frame, cell culturing bracket and cell culture system, bracing frame is formed with supporting mouth, supporting mouth and cell are cultivated fibrous membrane and are matched, and supporting mouth is for supporting the edge one week of immobilized-cell culture fibrous membrane, the edge supporting cell cultivation fibrous membrane thus can be fixed by the supporting mouth on bracing frame, when employing common law in culture vessel during perfusion cell solution, cell cultivate fibrous membrane because of on bracing frame the support of supporting mouth will not produce shrinkage, and the cell in solution can be evenly affixed to cell and cultivate on fibrous membrane, each cell all can absorb the nutrient in culture medium, thus reduce the difficulty that cell is cultivated and detected, and compared to dibbling method of the prior art, common-law seeded process is simple, consuming time shorter, efficiency is higher.
Accompanying drawing explanation
In order to be illustrated more clearly that this utility model embodiment or technical scheme of the prior art, the accompanying drawing used required in embodiment or description of the prior art will be briefly described below, apparently, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is one of structural representation of this utility model embodiment cell cultivation fibrous membrane bracing frame；
Fig. 2 is that this utility model embodiment cell cultivates the two of the structural representation of fibrous membrane bracing frame；
Fig. 3 is the structural representation of retainer ring in this utility model embodiment cell culturing bracket.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is clearly and completely described, it is clear that described embodiment is only a part of embodiment of this utility model, rather than whole embodiments.Based on the embodiment in this utility model, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of this utility model protection.
In description of the present utility model, it will be appreciated that, orientation or the position relationship of the instruction such as term " " center ", " on ", D score, "front", "rear", "left", "right", " vertically ", " level ", " top ", " end ", " interior ", " outward " be based on orientation shown in the drawings or position relationship; be for only for ease of description this utility model and simplifying and describe; rather than instruction or imply indication device or element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to restriction of the present utility model.In description of the present utility model, except as otherwise noted, " multiple " are meant that two or more.
With reference to Fig. 1, Fig. 1 is the specific embodiment that this utility model embodiment cell cultivates fibrous membrane bracing frame, the cell of the present embodiment is cultivated fibrous membrane bracing frame and is used for supporting cell cultivation fibrous membrane (not shown), described cell is cultivated fibrous membrane and is cultivated for cell, support frame as described above 1 is formed with supporting mouth 2, described supporting mouth 2 and described cell are cultivated fibrous membrane and are matched, and described supporting mouth 2 cultivates the edge one week of fibrous membrane for supporting fixing described cell.
A kind of cell that this utility model embodiment provides cultivates fibrous membrane bracing frame, bracing frame 1 is formed with supporting mouth 2, supporting mouth 2 and cell are cultivated fibrous membrane and are matched, and supporting mouth 2 is for supporting the edge one week of immobilized-cell culture fibrous membrane, thus can pass through the fixing cell that supports of the supporting mouth 2 on bracing frame 1 and cultivate the edge of fibrous membrane, when employing common law in culture vessel during perfusion cell solution, cell cultivate fibrous membrane because of on bracing frame 1 support of supporting mouth 2 will not produce shrinkage, and the cell in solution can be evenly affixed to cell and cultivate on fibrous membrane, each cell all can absorb the nutrient in culture medium, thus reduce the difficulty that cell is cultivated and detected, and compared to dibbling method of the prior art, common-law seeded process is simple, consuming time shorter, efficiency is higher.
In the above-described embodiments, the shape of supporting mouth 2 can be circular, square, polygon etc., is not specifically limited at this.
Wherein, bracing frame 1 can be circulus, the hollow space of circulus is supporting mouth 2, after the edge of cell cultivation fibrous membrane is fixedly connected on one week of this supporting mouth 2 for one week, this circulus entirety can be put into culture vessel, and in culture vessel, irrigate cell solution to carry out cell cultivation.But, now, take out by culture vessel in order to the cell on bracing frame 1 is cultivated fibrous membrane, tweezers need to be stretched into and take out bracing frame 1 in culture vessel and take out of so that cell is cultivated fibrous membrane, and when the opening that culture vessel has the bigger degree of depth or culture vessel is less, this gripping operation will extremely inconvenience.Therefore, in order to avoid this problem, preferably, bracing frame 1 can be made as structure as shown in Figure 1 or 2, namely, bracing frame 1 is tubular structure, supporting mouth 2 is the one end open of tubular structure, the other end opening part of tubular structure is along the formation mounting part 3 that extends radially outwardly of this tubular structure, thus by this mounting part 3, cell can be cultivated fibrous membrane and be mounted on culture vessel opening part, when this cell is cultivated fibrous membrane taking-up by needs, can adopt tweezers, in the mounting part 3 that the opening part of culture vessel is clamped on bracing frame 1, cell can be cultivated fibrous membrane to be taken out by inside culture vessel, grip inside culture vessel compared to stretching into, operation easier is gripped relatively low at culture vessel opening part.
It should be noted that, mounting part 3 can be the edge one week enclosing as shown in Figure 1 or 2 and being located at tubular structure, can also for be uniformly arranged on tubular structure edge multiple projections of a week, can also is that other structures, supporting mouth 2 do not limit at this, as long as can be mounted on the opening part of culture vessel.
Specifically, bracing frame 1 can be cylindrical barrel structure, prismatic tubular structure or taper type tubular structure etc., does not limit at this.But, in order to enable the one end arranging supporting mouth 2 on bracing frame 1 to stretch in culture vessel through the opening of culture vessel relatively easily, preferably, bracing frame 1 can be made as taper type tubular structure as shown in Figure 1, and taper type tubular structure defines on less bottom surface supporting mouth 2, thus bracing frame 1 is small top and big bottom structure, arranges one end of supporting mouth 2, namely one end that area is less, it is possible to stretch into relatively easily in culture vessel.
Tweezers gripping is adopted in order to convenient, preferably, as shown in Figure 1 or 2, the outer edge of mounting part 3 is provided with outward extending handle 4, thus can clamp the both sides of handle 4 by tweezers and put into culture vessel by taking out or cell is cultivated fibrous membrane in culture vessel so that cell to be cultivated fibrous membrane.This simple in construction, easy to operate.It is additionally, since handle 4 and is arranged at the outer edge of mounting part 3, and stretch out, need to when the upper end open place lid of tubular structure set upper cover in culture vessel if therefore preventing the dust water in air from entering, handle 4 will not produce to interfere with this upper cover.
Cultivate fibrous membrane due to cell to be used for supporting cell, and bracing frame 1 is mainly used in supporting this cell and cultivates fibrous membrane, therefore to prevent the competent cell on cell cultivation fibrous membrane from migrating to bracing frame 1 have impact on the follow-up accuracy of cell detection on cell cultivation fibrous membrane, it is possible to bracing frame 1 to be made as the structure being unfavorable for that Growth of Cells is bred.In order to bracing frame 1 being made as the structure being unfavorable for that Growth of Cells is bred, preferably, bracing frame 1 is by polylactic acid fused glass pellet, owing to polylactic acid has hydrophobicity, and not there is hydrophilic, therefore cell is difficult to be attached on this material and grows, and therefore cannot be used for supporting cell, thus prevents competent cell to the transfer on bracing frame 1.And, fused glass pellet technology belongs to the one in 3D printing technique, there is the advantages such as shaped article precision is high, surface quality is good, simultaneously because poly-lactic acid material has the advantages such as nonhazardous, the bracing frame 1 after by polylactic acid fused glass pellet is made to have the characteristic such as precision height, good, the non-environmental-pollution of surface quality.
This utility model embodiment additionally provides a kind of cell culturing bracket, including: cell cultivates fibrous membrane, and described cell is cultivated fibrous membrane and is used for carrying cell；Bracing frame, support frame as described above is the bracing frame described in any of the above-described technical scheme, and the edge of described cell cultivation fibrous membrane is fixedly connected on the supporting mouth place of support frame as described above for one week.
Bracing frame owing to using in a kind of cell culturing bracket of the present embodiment is identical with the bracing frame provided in each embodiment of above-mentioned cell cultivation fibrous membrane bracing frame, and therefore the two can solve the problem that identical technical problem, and reaches identical Expected Results.
When cell cultivates fibrous membrane by electrostatic spinning process molding, conventional electrostatic spinning received vector is aluminium foil, when fiber after electrostatic spinning molding falls on aluminium foil will with produce between aluminium foil bonding, thus when isolating cell on aluminium foil and cultivating fibrous membrane, aluminium foil and cell are cultivated and are produced adhesion between fibrous membrane, easily widen cell and cultivate the pore-size of fibrous membrane, thus easily affecting cell to cultivate the result of use of fibrous membrane.Therefore, in order to avoid the problems referred to above, preferably, the received vector of electrostatic spinning process is buffer, cell after electrostatic spinning molding cultivates fibrous membrane due to lighter weight, thinner thickness, therefore may float on this buffer, only need to adopting tweezers that cell is cultivated fibrous membrane can realize cell and cultivate separating of fibrous membrane and buffer by taking out in solution and clean up, cell is cultivated the pore-size of fibrous membrane and is not changed.And, owing to conventional buffer is to cytotoxic evil effect, failing to wash this solution completely even if therefore taking out in buffer after cell cultivates fibrous membrane, when adopting this cell to cultivate fibrous membrane carrying cell, producing large effect without cell growth.
Wherein, the material of electrostatic spinning process molding can be pla-pcl or pla-pcl-collagen, is not specifically limited at this.Wherein, pla-pcl has good biocompatibility, biological degradability, medicine by property, the characteristic such as easy processing, and this material degrade in vivo after product to living organism nonhazardous effect, therefore can cultivate the material of fibrous membrane as electrostatic spinning constitutive cell.Pla-pcl-collagen is except having pla-pcl have the advantage that, and owing to there is collagen, collagen has certain hydrophilic, is therefore more conducive to the growth of cell.
Cultivate a kind of concrete fixing connected mode at fibrous membrane edge as supporting mouth 2 and cell, cell is cultivated the edge of fibrous membrane and is adhered to supporting mouth 2 place by medical adhesive in one week.This simple in construction, and medical adhesive has no side effect, and the growth of cell will not be produced impact.
As the fixing connected mode that supporting mouth 2 is concrete with the another kind at cell cultivation fibrous membrane edge, cell culture system also includes retainer ring 5, this retainer ring 5 is by polylactic acid fused glass pellet, retainer ring 5 engagement sleeves is located at described supporting mouth 2 place, and the edge for cell is cultivated fibrous membrane is pressed in the wall outer surface of supporting mouth 2 for one week.This simple in construction, convenient disassembly, be conducive to cell to cultivate the replacing of fibrous membrane.
Concrete, retainer ring 5 includes supporting ring body 51 and being uniformly arranged on and supports multiple protruding the 52 of inner wall of ring body one week.When retainer ring 5 engagement sleeves is located on the wall outer surface of supporting mouth 2, this projection 52 is in impaction state, the wall outer surface of supporting mouth 2 thus can apply certain extruding force and cultivate the edge one week of fibrous membrane with packed cell.This simple in construction, it is easy to realize.
When carrying out cell and cultivating, the bottom that cell can be incubated at culture vessel (can directly be incubated at the bottom of culture vessel, cell can also be incubated on the cell cultivation fibrous membrane that bottom of culture vessel is laid), in order to compare the advantage of this utility model cell culture system, cell can be incubated at respectively on the fibrous membrane in bottom of culture vessel and this utility model cell culture system, to carry out contrast experiment.In order to facilitate the comparison of contrast and experiment, preferably, in cell culture system, the area of fibrous membrane should be equal with the floor space of contrast experiment's culture vessel, make in be compared two groups of experiments for carrying the area equation of cell, to facilitate other cells to cultivate the comparison of parameter (such as cell yield), thus facilitating the comparison of two experimental results.
Wherein, owing to contrast experiment can be individually make with culture vessel, it can also be the culture vessel that laboratory is conventional, the conventional culture vessel of laboratory can be culture bottle, culture dish or culture plate etc., these culture vessels are respectively provided with specific model, therefore to avoid additionally making culture vessel, save cost, in cell culture system, the area of fibrous membrane should be equal with the floor space of the conventional culture vessel of laboratory, the culture vessel that thus contrast experiment adopts can select the culture vessel that laboratory is conventional, can avoid additionally making culture vessel, thus providing cost savings.Such as, when the culture vessel in cell culture system is six orifice plate, on six orifice plates, the area of the cultivation of the cell in culture hole fibrous membrane can with the culture hole area equation on 12 orifice plates, in order to adopt the culture vessel that 12 orifice plates are tested as a comparison.
This utility model embodiment additionally provides a kind of cell culture system, including: culture vessel；Cell culturing bracket, described cell culturing bracket is the cell culturing bracket described in any of the above-described technical scheme, and in described cell culturing bracket, the supporting mouth of bracing frame stretches in described culture vessel and arranges.
Cell culturing bracket owing to using in a kind of cell culture system of the present embodiment is identical with the cell culturing bracket provided in each embodiment of above-mentioned cell culturing bracket, and therefore the two can solve the problem that identical technical problem, and reaches identical Expected Results.
In the above-described embodiments, cell culturing bracket can entirety be positioned in culture vessel, a part can also be positioned at culture vessel, another part stretches out the opening of culture vessel and hangs on this opening part, it is not specifically limited at this, as long as the supporting mouth 2 on this cell culturing bracket is positioned at culture vessel.
The nutrient in culture medium is better absorbed in order to enable cell to cultivate cell on fibrous membrane, preferably, the mounting part 3 of bracing frame 1 is mounted on the opening part of culture vessel, cell is cultivated fibrous membrane and is suspended in culture vessel, thus cell is cultivated the cell on fibrous membrane and can be absorbed nutrient that cell cultivates above fibrous membrane in culture medium and the nutrient that cell is cultivated below fibrous membrane in culture medium simultaneously, is more conducive to the growth of cell.And, cultivate fibrous membrane due to cell and be suspended in culture vessel, therefore, two kinds of cells can be cultivated in this culture vessel, namely, a kind of cell is incubated at cell and cultivates the upper surface of fibrous membrane, and another kind of cell is incubated in the space that cell is cultivated between fibrous membrane lower surface and the bottom of culture vessel, thereby increases the space availability ratio of culture vessel.
Wherein, cell is cultivated in the space between lower surface and the bottom of culture vessel of fibrous membrane and can be carried out cell suspension cultures, it is also possible to carries out cell attachment cultivation, is not specifically limited at this.
It should be noted that, when cell cultivation fibrous membrane is suspended in culture vessel, the process adding cell solution in culture vessel can be: first adds a certain amount of culture medium in culture vessel, secondly the bracing frame being fixedly connected with cell cultivation fibrous membrane is hung on the opening part of culture vessel, one end of bracing frame 1 immobilized-cell culture fibrous membrane is stretched in culture vessel, then irrigating cell solution in bracing frame 1 hollow region of tubular structure, the cell in cell solution can sink down into cell under the effect of its gravity and cultivate on fibrous membrane.Thus, cell can be made to cultivate fibrous membrane both sides and to keep pressure balance, it is prevented that the pressure of side is excessive and makes cell cultivate fibrous membrane and is separated with supporting mouth under the impact of this pressure.
It addition, culture vessel can be the culture dish, culture bottle or the culture plate that generally adopt in laboratory, it is also possible to be the culture vessel of other designs temporarily, be not specifically limited at this.But, in order to adopt culture vessel to do organize experiment simultaneously, culture vessel can be culture plate more, culture plate is provided with multiple culture hole, it is provided with cell culturing bracket in each culture hole, this culture plate therefore can be adopted simultaneously to do and organize experiment more, thus improve the utilization rate of culture vessel.Additionally, the entirety that culture vessel can also connect into for multiple containers, culture vessel can be adopted equally to do many group experiments utilization rate to improve culture vessel simultaneously.
The above; it is only detailed description of the invention of the present utility model; but protection domain of the present utility model is not limited thereto; any those familiar with the art is in the technical scope that this utility model discloses; change can be readily occurred in or replace, all should be encompassed within protection domain of the present utility model.Therefore, protection domain of the present utility model should be as the criterion with described scope of the claims.
Claims (10)
1. a cell cultivates fibrous membrane bracing frame, fibrous membrane is cultivated for supporting cell, described cell is cultivated fibrous membrane and is cultivated for cell, it is characterized in that, support frame as described above is formed with supporting mouth, described supporting mouth and described cell are cultivated fibrous membrane and are matched, and described supporting mouth cultivates the edge one week of fibrous membrane for supporting fixing described cell.
2. cell according to claim 1 cultivates fibrous membrane bracing frame, it is characterized in that, support frame as described above is tubular structure, and described supporting mouth is the one end open of described tubular structure, and the other end opening part of described tubular structure is along the formation mounting part that extends radially outwardly of described tubular structure.
3. cell according to claim 2 cultivates fibrous membrane bracing frame, it is characterized in that, support frame as described above is taper type tubular structure, and bottom surface less in described taper type tubular structure is formed described supporting mouth, and the outer edge of described mounting part is provided with outward extending handle.
4. cell according to claim 1 cultivates fibrous membrane bracing frame, it is characterised in that support frame as described above is by polylactic acid fused glass pellet.
5. a cell culturing bracket, it is characterised in that including:
Cell cultivates fibrous membrane, and described cell is cultivated fibrous membrane and is used for carrying cell；
Bracing frame, support frame as described above is the bracing frame according to any one of Claims 1 to 4, and the edge of described cell cultivation fibrous membrane is fixedly connected on the supporting mouth place of support frame as described above for one week.
6. cell culturing bracket according to claim 5, it is characterised in that described cell is cultivated fibrous membrane and passed through electrostatic spinning process molding by polycaprolactone or polycaprolactone-collagen-based materials, and the received vector of electrostatic spinning process is buffer.
7. cell culturing bracket according to claim 5, it is characterized in that, also include retainer ring, described retainer ring is by polylactic acid fused glass pellet, described retainer ring engagement sleeves is located at described supporting mouth place, and the edge for described cell is cultivated fibrous membrane is pressed in the wall outer surface of described supporting mouth for one week.
8. a cell culture system, it is characterised in that including:
Culture vessel；
Cell culturing bracket, described cell culturing bracket is the cell culturing bracket according to any one of claim 5～7, and in described cell culturing bracket, the supporting mouth of bracing frame stretches in described culture vessel and arranges.
9. cell culture system according to claim 8, it is characterised in that the mounting part of support frame as described above is mounted on the opening part of described culture vessel, described cell is cultivated fibrous membrane and is suspended in described culture vessel.
10. cell culture system according to claim 8, it is characterised in that described culture vessel is culture plate, described culture plate is provided with multiple culture hole, is provided with described cell culturing bracket in each described culture hole.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620066733.XU CN205347445U (en) | 2016-01-22 | 2016-01-22 | Cell culture fibre membrane support frame, cell culture support and cell culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620066733.XU CN205347445U (en) | 2016-01-22 | 2016-01-22 | Cell culture fibre membrane support frame, cell culture support and cell culture device |
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| CN205347445U true CN205347445U (en) | 2016-06-29 |
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| CN201620066733.XU Expired - Fee Related CN205347445U (en) | 2016-01-22 | 2016-01-22 | Cell culture fibre membrane support frame, cell culture support and cell culture device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300713A (en) * | 2017-12-31 | 2018-07-20 | 宁波大学 | The method and device of fixed cell |
| CN109294913A (en) * | 2018-10-29 | 2019-02-01 | 深圳大学 | A kind of membrane material fixed card buckle suitable for tissue culture plate |
| CN110656048A (en) * | 2019-11-14 | 2020-01-07 | 广州瑞铂茵健康管理咨询有限公司 | Cell culture cell and have its culture vessel |
| CN111065727A (en) * | 2017-07-13 | 2020-04-24 | 阿莫生命科学有限公司 | Cell culture container |
-
2016
- 2016-01-22 CN CN201620066733.XU patent/CN205347445U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111065727A (en) * | 2017-07-13 | 2020-04-24 | 阿莫生命科学有限公司 | Cell culture container |
| CN111065727B (en) * | 2017-07-13 | 2023-09-12 | 阿莫生命科学有限公司 | Cell culture vessel |
| CN108300713A (en) * | 2017-12-31 | 2018-07-20 | 宁波大学 | The method and device of fixed cell |
| CN109294913A (en) * | 2018-10-29 | 2019-02-01 | 深圳大学 | A kind of membrane material fixed card buckle suitable for tissue culture plate |
| CN110656048A (en) * | 2019-11-14 | 2020-01-07 | 广州瑞铂茵健康管理咨询有限公司 | Cell culture cell and have its culture vessel |
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