CN209162085U - A kind of fermentor of animal cell culture - Google Patents
A kind of fermentor of animal cell culture Download PDFInfo
- Publication number
- CN209162085U CN209162085U CN201821487959.2U CN201821487959U CN209162085U CN 209162085 U CN209162085 U CN 209162085U CN 201821487959 U CN201821487959 U CN 201821487959U CN 209162085 U CN209162085 U CN 209162085U
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- China
- Prior art keywords
- fermentor
- supervisor
- cell culture
- branch pipe
- animal cell
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Abstract
The utility model discloses a kind of fermentors of animal cell culture, including tank body, blow-off valve is arranged in tank base, top of the tank is provided with inlet, the tank body internal upper part is removably connected with partition, several through-holes are provided on the partition, the through-hole is arranged in concentric circles, each through-hole is fixed with a filter holder, a filter membrane bag is arranged on the filter holder, distributor is provided with above the partition, the distributor includes supervisor and is in the branch pipe that divergent shape is arranged along supervisor's circumference, the branch pipe is connected with supervisor, the supervisor is connected by feed pipe with feed liquid storage tank, high-voltage pulse pump is provided on the feed pipe, there are multiple nozzles in the branch pipe lower part along the axial spaced set of branch pipe, the position of the nozzle and the position of through-hole correspond, the utility model is realized dynamic Object adhere-wall culture, and the separation without realizing cell and filter membrane bag in the case where mechanical means.
Description
Technical field
The utility model relates to a kind of fermentors of animal cell culture.
Background technique
Currently, the common method of cell culture has: spinner culture, bioreactor culture.
Spinner culture is traditional attached cell culture technique, due to manual operation heavy workload, opens bottleneck repeatedly and leads
Cause pollution risk high, production technology poor controllability, difference is big between bottle, batch definition is difficult unified, at present spinner culture gradually by
It eliminates.
Bioreactor culture method makes cell be attached at chip carrier or spherical microcarrier in the bioreactor
Surface and grow, cell attachment is influenced in fixed surface, not by stirring, and culture solution can be easily changed, and productivity is high,
But current applicable bioreactor cannot achieve piping and druming or use scraper supplementary means, it is difficult to by cell from
Chip carrier or spherical microcarrier all digest.
Utility model content
Purpose of utility model: it is dynamic that purpose of the utility model is to solve the deficiencies of the above prior art and provide one kind
The fermentor of object cell culture.
Technical solution: a kind of fermentor of animal cell culture described in the utility model, including tank body, tank base are set
Blow-off valve is set, tank body lower part is provided with inoculation mouth, and the tank body internal upper part is removably connected with partition, sets on the partition
Several through-holes are equipped with, the through-hole is arranged in concentric circles, and each through-hole is fixed with a filter holder, the filter membrane branch
It is arranged with a filter membrane bag on frame, distributor is provided with above the partition, the distributor includes supervisor and justifies along supervisor
The branch pipe that week arranges in divergent shape, the branch pipe are connected with supervisor, and the supervisor is connected by feed pipe with feed liquid storage tank, institute
High-voltage pulse pump is provided on the feed pipe stated, the branch pipe lower part there are multiple sprays along the axial spaced set of branch pipe
Mouth, the position of the nozzle and the position of through-hole correspond.
Preferably, the fermentor is coated with heating mantle.
Preferably, temperature sensor, pH sensor, pressure sensor and dissolved oxygen sensing are provided in the fermentor
Device.
Preferably, the fermentor tube body is tempered glass material.
Preferably, the diameter of the tank body lower part is diminishing.
The utility model has the advantages that being provided with several filter membrane bags in fermentor described in the utility model, it is all provided with above each filter membrane bag
Set a nozzle, zooblast is grown close to filter membrane bag when culture, and after fermentation, feed liquid is squeezed into point by high-voltage pulse pump
It in cloth device, is sprayed into filter membrane bag by the nozzle on distributor, impacts the outside upper cell of filter membrane bag, realize point of cell and filter membrane bag
From being completed at the same time the dilution of cell.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the structural schematic diagram of partition.
Fig. 3 is the top view illustration of distributor.
Specific embodiment
In order to deepen the understanding to the utility model, the utility model is made below in conjunction with embodiment and attached drawing further
It is described in detail, which is only used for explaining the utility model, does not constitute the restriction to scope of protection of the utility model.
Referring to Fig. 1, a kind of fermentor of animal cell culture, including tank body 1, tank base are provided with blow-off valve, tank body
Lower part is provided with inoculation mouth, and the tank body internal upper part is removably connected with partition 2, several through-holes are provided on the partition
3, the through-hole is arranged in concentric circles, and each through-hole 3 is fixed with a filter holder, is arranged on the filter holder
One filter membrane bag 10, the partition top are provided with distributor, and the distributor includes supervisor 4 and is in dissipate along supervisor's circumference
The branch pipe 5 of shape arrangement, the branch pipe 5 are connected with supervisor 4, and the supervisor is connected by feed pipe 6 with feed liquid storage tank 7, described
Feed pipe on be provided with high-voltage pulse pump 8, there are multiple nozzles in the branch pipe lower part along the axial spaced set of branch pipe
9, the position of the nozzle 9 and the position of through-hole 3 correspond.
Preferably, heating mantle (not illustrating) is provided with outside the fermentor.
Preferably, temperature sensor, pH sensor, pressure sensor and dissolved oxygen sensing are provided in the fermentor
Device (does not illustrate).
In order to observe the growing state of fermentor inner cell, the fermentor tube body is tempered glass material.
Preferably, the tank body lower part is cone.
When culture, zooblast is grown close to filter membrane bag, after the completion of cell culture, harvests cell by following step sequence:
1) bottom valve is opened, culture medium is discharged, drained culture medium closes bottom valve；
2) add pancreatin digestive juice from feed inlet, squeezed into point using high-voltage pulse stock liquid (depending on reduction of feed volume is by extension rate)
It in cloth device, is sprayed into filter membrane bag by the nozzle on distributor, impacts the attached cell outside filter membrane bag, realize cell and filter membrane bag
Separation, then open bottom valve, harvest cell suspending liquid.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Within the spirit and principle of utility model, any modification, equivalent replacement, improvement and so on should be included in the utility model
Protection scope within.
Claims (5)
1. a kind of fermentor of animal cell culture, which is characterized in that including tank body, tank base is arranged blow-off valve, under tank body
Portion is provided with inoculation mouth, and the tank body internal upper part is removably connected with partition, and several through-holes, institute are provided on the partition
The through-hole stated is arranged in concentric circles, and each through-hole is fixed with a filter holder, a filter is arranged on the filter holder
Film bag, the partition top are provided with distributor, and the distributor includes supervisor and arranges along supervisor's circumference in divergent shape
Branch pipe, which is connected with supervisor, and the supervisor is connected by feed pipe with feed liquid storage tank, sets on the feed pipe
It is equipped with high-voltage pulse pump, there are multiple nozzles, the position of the nozzle in the branch pipe lower part along the axial spaced set of branch pipe
It sets and is corresponded with the position of through-hole.
2. a kind of fermentor of animal cell culture according to claim 1, which is characterized in that the fermentor outsourcing
It is covered with heating mantle.
3. a kind of fermentor of animal cell culture according to claim 1, which is characterized in that set in the fermentor
It is equipped with temperature sensor, pH sensor, pressure sensor and dissolved oxygen sensor.
4. a kind of fermentor of animal cell culture according to claim 2, which is characterized in that the fermentor tube body
For tempered glass material.
5. a kind of fermentor of animal cell culture according to claim 1, which is characterized in that the tank body lower part
Diameter is diminishing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821487959.2U CN209162085U (en) | 2018-09-12 | 2018-09-12 | A kind of fermentor of animal cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821487959.2U CN209162085U (en) | 2018-09-12 | 2018-09-12 | A kind of fermentor of animal cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209162085U true CN209162085U (en) | 2019-07-26 |
Family
ID=67331015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821487959.2U Active CN209162085U (en) | 2018-09-12 | 2018-09-12 | A kind of fermentor of animal cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209162085U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111849774A (en) * | 2020-08-17 | 2020-10-30 | 陈红 | Adherent cell culture fermentation cylinder |
-
2018
- 2018-09-12 CN CN201821487959.2U patent/CN209162085U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111849774A (en) * | 2020-08-17 | 2020-10-30 | 陈红 | Adherent cell culture fermentation cylinder |
| CN111849774B (en) * | 2020-08-17 | 2023-07-14 | 陈红 | Wall-attached cell culture fermentation tank |
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