CN111849774B

CN111849774B - Wall-attached cell culture fermentation tank

Info

Publication number: CN111849774B
Application number: CN202010825449.7A
Authority: CN
Inventors: 陈红
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-08-17
Filing date: 2020-08-17
Publication date: 2023-07-14
Anticipated expiration: 2040-08-17
Also published as: CN111849774A

Abstract

The invention discloses an adherent cell culture fermentation tank, which comprises a fermentation tank protection shell, a fermentation tank body and a cell counting mechanism, wherein the cell counting mechanism is positioned at a position, close to the middle, of the front end of the fermentation tank protection shell, and penetrates through a cavity to the interior of the fermentation tank body, and the fermentation tank body is positioned in the fermentation tank protection shell; according to the invention, through the cooperation of the fixing ring, the first guide pipe, the conveying pipe, the stirring rod, the stirring shaft, the plurality of groups of stirring blades and the shaft body of the downward extending shaft, the shearing action of the stirring device on cells in the traditional scheme is reduced, the cell counting mechanism is convenient for people to control different cultivation stages in the cell cultivation process, different pipelines connected with the first valve are used for conveying different substances, the condition that only the required substances are conveyed each time for cell cultivation is ensured, the probability of confusion among the materials is reduced, the pre-experiment of the cell cultivation by a single variable is changed, and the cell cultivation process is optimized.

Description

Wall-attached cell culture fermentation tank

Technical Field

The invention relates to the technical field of cells, in particular to an adherent cell culture fermentation tank.

Background

Cells are the basic units of organisms in biology, and mainly comprise cell nuclei and cytoplasm, the surfaces of the cells are provided with cell membranes, the proliferation of the cells is realized through the division of the cells, and the cells which are continuously divided are in a cell cycle from the time of completing one division to the time of completing the next division. There are four ways of cell division, among which there are 3 eukaryotic cells: mitosis, amitoses and meiosis, the first two being somatic divisions; whereas prokaryotic cells divide in a binary manner, cells such as adherent cells must grow on a surface of a support to which they adhere, and cells rely on attachment factors secreted by themselves or provided in culture to grow and reproduce on that surface.

In the existing scheme, the condition that the stirring device shears cells frequently occurs in the process of cell culture, so that the cell culture yield is low, meanwhile, the production cost is also improved, single variable control is not carried out on culture components in the existing scheme, the pre-experiment is not facilitated, after the technological process is determined, the dead plate follows the technological process to carry out production operation, the technological process is rarely subjected to intensive research, in addition, in the existing scheme, different stages of cell culture can not be intuitively reflected, different requirements on the composition of nutritional components and the proportion of each component in the different stages of cell culture are different, and the condition that cells stagnate and grow in the different stages of cell culture can not be controlled can be avoided.

Disclosure of Invention

The invention aims to provide an adherent cell culture fermentation tank, which solves the technical problems that:

the fixed ring, the first conduit, the conveying pipe, the stirring rod, the stirring shaft, the plurality of groups of stirring blades and the downward extending shaft body are matched for use, so that the shearing action of the stirring device on cells in the traditional scheme is reduced, various components in the fermentation tank are uniformly mixed, cell culture is facilitated, the sheet-shaped carrier provides sufficient surface area for the growth of the cells, the sheet-shaped carrier can be recycled, the problem that the stirring device shears the cells in the traditional scheme is solved, the production efficiency is improved, the production cost is reduced, the cell concentration data is collected through the capacitance sensor, and the problem that people cannot control the different culture stages in the cell culture process is solved;

one side of the first valve, which is far away from the fermentation tank body, is respectively connected with a culture solution pipe, a carbon dioxide pipe, an alkali liquor pipe, a steam pipe, a sterile air pipe and a cooling water pipe, and different pipelines are used for conveying different substances, so that the condition that only the required substances are conveyed each time for cell culture is ensured, the probability of confusion among the materials is reduced, the problem that a single variable cannot be changed for carrying out a pre-experiment on the cell culture is solved, and the optimization of a cell culture process is facilitated.

The aim of the invention can be achieved by the following technical scheme:

an adherent cell culture fermentation tank comprises a fermentation tank protection shell, a fermentation tank body and a cell counting mechanism, wherein the cell counting mechanism is positioned at a position, close to the middle, of the front end of the fermentation tank protection shell, and penetrates through a cavity to the interior of the fermentation tank body, and the fermentation tank body is positioned in the fermentation tank protection shell;

the utility model provides a fermentation cylinder, including fermentation cylinder body and fermentation cylinder protective housing, be provided with the cavity between fermentation cylinder body and the fermentation cylinder protective housing, cell counting mechanism includes bottom plate, baffle, sleeve and capacitive sensor, the baffle is located between bottom plate and the sleeve, capacitive sensor all runs through bottom plate, baffle and telescopic inside, fermentation cylinder apron and rubber seal are installed to the upper end of fermentation cylinder body, rubber seal is located between fermentation cylinder body and the fermentation cylinder apron, the fixed block is installed to the position that the cavity is close to the upper end, lifting seat and circulating pipe are installed to one side of fermentation cylinder protective housing, the lifting seat is located the top of circulating pipe, the opposite side of fermentation cylinder protective housing is close to the position of upper end and is connected with the illumination casing, a plurality of sets of supporting legs are installed to fermentation cylinder protective housing's lower extreme symmetry, the inside of cavity is close to the position of below and is connected with two sets of spliced poles and sampling post, two sets of spliced poles are located between a plurality of supporting legs, the lower extreme of fermentation cylinder protective housing is close to the position of centre and is installed down and is stretched the axis body, sampling post and stretches down between the axis body and two sets of spliced poles, the sampling post is located one side of the cavity of two sets of all.

Further, two sets of conveyer pipes are installed to the position symmetry that the surface of fermentation cylinder body is close to both sides, the upper and lower end of conveyer pipe all runs through to the inside of fermentation cylinder body, the inside of fermentation cylinder body is close to the position symmetry in the middle of being provided with a plurality of solid fixed ring, the front end of fermentation cylinder protective housing installs the sight glass near the position of upper end, the sight glass runs through the inside of cavity to fermentation cylinder body, the inside of fermentation cylinder body is close to the position in the middle and installs the (mixing) shaft, the upper end of (mixing) shaft is connected with the connecting rod, the upper end welding of connecting rod has the stirring rod, the position that the (mixing) shaft is close to the top is connected with first baffle, the position that the (mixing) shaft is close to the below is connected with the second baffle, the surface mounting of (mixing) shaft has a plurality of sets of stirring vane, a plurality of stirring vane all is located between first baffle and the second baffle, the (mixing) shaft all runs through the inside of first baffle and second baffle.

Further, one side of the circulating pipe is horizontally connected with a return pipe, the return pipe is semicircular, the return pipe is vertically connected with a plurality of groups of first valves and second valves, and the first valves are located above the second valves.

Further, the reinforcing block is installed to one side of fermentation cylinder apron, the inside of fermentation cylinder apron is provided with a plurality of screw, the fermentation cylinder apron passes through the bolt and links to each other with the fixed block, two sets of spliced pole and sampling column all run through the inside of cavity to the fermentation cylinder body, the lower extreme of sampling column is connected with the sample bottle.

Further, one side of the first valve is connected with a first conduit, an illuminating lamp is arranged in the illuminating shell, one side of the illuminating shell, which is close to the fermentation tank body, is connected with a second conduit, and the first conduit and the second conduit penetrate through the chamber to the interior of the fermentation tank body.

Further, the surface of connecting rod and (mixing) shaft all is provided with the screw, connecting rod and (mixing) shaft pass through bolted connection, the surface of first baffle and second baffle all is connected with the sealing washer, all be provided with the sieve mesh on first baffle and the second baffle, the upper surface of first baffle is close to the position of both sides and is provided with a plurality of hollow casing string.

Further, the top of lifting seat is connected with first piston rod and second piston rod, first piston rod is located the top of second piston rod, the reinforcing block is close to the position in the middle and is provided with cylindrical through-hole, first piston rod is pegged graft in cylindrical through-hole's inside, just the upper surface of first piston rod and the upper surface of reinforcing block are in same horizontal plane.

The invention has the beneficial effects that:

the fixed ring, the first conduit, the conveying pipe, the stirring rod, the stirring shaft, the plurality of groups of stirring blades and the shaft body of the downward extending shaft are matched for use, so that the shearing action of the stirring device on cells in the traditional scheme is reduced, various components in the fermentation tank are uniformly mixed, cell culture is facilitated, the sheet carrier provides sufficient surface area for the growth of the cells, the sheet carrier can be recycled, the production efficiency is improved, the production cost is reduced, the collection of cell concentration data is carried out through the capacitance sensor, and people can conveniently control different culture stages in the cell culture process;

one side of the first valve, which is far away from the fermentation tank body, is respectively connected with a culture solution pipe, a carbon dioxide pipe, an alkali liquor pipe, a steam pipe, a sterile air pipe and a cooling water pipe, and different pipelines are used for conveying different substances, so that the condition that only the required substances are conveyed each time for cell culture is ensured, the probability of confusion between the materials is reduced, the pre-experiment of the single variable on the cell culture is changed, and the cell culture process is optimized.

Drawings

The present invention is further described below with reference to the accompanying drawings for the convenience of understanding by those skilled in the art.

FIG. 1 is a schematic diagram of an adherent cell culture fermenter according to the present invention;

FIG. 2 is an enlarged schematic view of the present invention at B;

FIG. 3 is a top view of a fermenter cover plate according to the present invention;

FIG. 4 is a cross-sectional view of a cell counting mechanism according to the present invention;

FIG. 5 is an enlarged schematic view of the invention at A;

fig. 6 is a top view of the return tube of the present invention.

In the figure: 1. a reinforcing block; 2. lifting the seat; 3. a fixed block; 4. a first valve; 5. a second valve; 6. a circulation pipe; 7. a fixing ring; 8. a fermenter protecting case; 9. a first separator; 10. a fermenter body; 11. a delivery tube; 12. a fermenter cover plate; 13. a stirring rod; 14. a return pipe; 15. an observation mirror; 16. a lighting housing; 17. a connecting rod; 18. sampling bottle; 19. stirring blades; 20. a stirring shaft; 21. a cell counting mechanism; 22. a second separator; 23. a connecting column; 24. a sampling column; 25. a downward extending shaft body; 211. a bottom plate; 212. a baffle; 213. a sleeve; 214. a capacitive sensor.

Detailed Description

The technical solutions of the present invention will be clearly and completely described in connection with the embodiments, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

1-6, an adherent cell culture fermentation tank comprises a fermentation tank protection shell 8, a fermentation tank body 10 and a cell counting mechanism 21, wherein the cell counting mechanism 21 is positioned at a position, close to the middle, of the front end of the fermentation tank protection shell 8, the cell counting mechanism 21 penetrates through a cavity to the interior of the fermentation tank body 10, and the fermentation tank body 10 is positioned in the fermentation tank protection shell 8;

the fermentation cylinder body 10 is provided with the cavity between the fermentation cylinder protection casing 8, cell counting mechanism 21 includes bottom plate 211, baffle 212, sleeve 213 and electric capacity sensor 214, baffle 212 is located between bottom plate 211 and the sleeve 213, electric capacity sensor 214 all runs through the inside of bottom plate 211, baffle 212 and sleeve 213, fermentation cylinder apron 12 and rubber seal are installed to the upper end of fermentation cylinder body 10, rubber seal is located between fermentation cylinder body 10 and the fermentation cylinder apron 12, fixed block 3 is installed to the position that the cavity is close to the upper end, lifting seat 2 and circulating pipe 6 are installed to one side of fermentation cylinder protection casing 8, lifting seat 2 is located the top of circulating pipe 6, the opposite side of fermentation cylinder protection casing 8 is close to the position of upper end and is connected with illumination casing 16, a plurality of sets of supporting legs are installed to the lower extreme symmetry of fermentation cylinder protection casing 8, the inside position that is close to the below of cavity is connected with two sets of spliced poles 23 and sampling column 24, two sets of spliced pole 23 are located between a plurality of sets of supporting legs, the position that the fermentation cylinder protection casing 8 is close to the lower shaft 25 is located between two sets of spliced pole 24, the lower shaft 25 is located between two sets of spliced pole 25 and the same, and the lower shaft 25 of the fermentation cylinder protection casing is located between two sets of spliced pole 25.

Two sets of conveyer pipes 11 are installed to the position symmetry that the surface of fermentation cylinder body 10 is close to both sides, the inside to fermentation cylinder body 10 is all run through to the upper and lower end of conveyer pipe 11, the position symmetry that the inside to fermentation cylinder body 10 is close to the centre is provided with a plurality of solid fixed ring 7, the front end of fermentation cylinder protection casing 8 is close to the position of upper end and installs sight glass 15, sight glass 15 runs through the inside of cavity to fermentation cylinder body 10, the inside to the centre of fermentation cylinder body 10 is installed stirring axle 20, the upper end of stirring axle 20 is connected with connecting rod 17, the upper end welding of connecting rod 17 has stirring rod 13, the position that stirring axle 20 is close to the top is connected with first baffle 9, the position that stirring axle 20 is close to the below is connected with second baffle 22, the surface mounting of stirring axle 20 has a plurality of sets of stirring vane 19, a plurality of sets of stirring vane 19 all are located between first baffle 9 and the second baffle 22, stirring axle 20 all runs through the inside of first baffle 9 and second baffle 22.

One side of the circulating pipe 6 is horizontally connected with a return pipe 14, the return pipe 14 is semicircular, the return pipe 14 is vertically connected with a plurality of groups of first valves 4 and second valves 5, and the first valves 4 are positioned above the second valves 5.

The reinforcing block 1 is installed to one side of fermentation cylinder apron 12, the inside of fermentation cylinder apron 12 is provided with a plurality of screw, fermentation cylinder apron 12 passes through the bolt and links to each other with fixed block 3, two sets of spliced pole 23 and sampling post 24 all run through the inside of cavity to fermentation cylinder body 10, the lower extreme of sampling post 24 is connected with sampling bottle 18.

One side of the first valve 4 is connected with a first conduit, an illuminating lamp is arranged in the illuminating shell 16, one side of the illuminating shell 16, which is close to the fermentation tank body 10, is connected with a second conduit, and the first conduit and the second conduit penetrate through the chamber to the interior of the fermentation tank body 10.

The surface of connecting rod 17 and (mixing) shaft 20 all is provided with the screw, connecting rod 17 and (mixing) shaft 20 pass through bolted connection, the surface of first baffle 9 and second baffle 22 all is connected with the sealing washer, all be provided with the sieve mesh on first baffle 9 and the second baffle 22, the upper surface of first baffle 9 is close to the position of both sides and is provided with a plurality of hollow casing string.

The lifting seat is characterized in that a first piston rod and a second piston rod are connected above the lifting seat 2, the first piston rod is located above the second piston rod, a cylindrical through hole is formed in the position, close to the middle, of the reinforcing block 1, the first piston rod is inserted into the cylindrical through hole, and the upper surface of the first piston rod and the upper surface of the reinforcing block 1 are located on the same horizontal plane.

Example 1

A cavity is arranged between the fermentation tank body 10 and the fermentation tank protection shell 8, two groups of conveying pipes 11 are symmetrically arranged on the outer surface of the fermentation tank body 10 close to the two sides, the upper end and the lower end of each conveying pipe 11 penetrate through the interior of the fermentation tank body 10, a hose is connected to the upper port of each conveying pipe 11, penetrates through the fixing ring 7 and is connected with the first conduit, so that uniform mixing of various components in the fermentation tank is facilitated, cell culture is facilitated, a first piston rod and a second piston rod are connected above the lifting seat 2, a hydraulic pump is arranged in the lifting seat 2, the first piston rod and the second piston rod are in sliding connection, the first piston slides upwards under the action of the hydraulic pump until the fermentation tank cover plate 12 completely leaves the fermentation tank body 10, the fermentation tank cover plate 12 can rotate through the first piston rod, the first partition plate 9, the stirring rod 13, the stirring shaft 20, the second partition plate 22 and the hose can be detached conveniently after fermentation is finished, and the type of the hydraulic pump can be LT-SDYG;

the first baffle plate 9 and the second baffle plate 22 are provided with sieve holes, which are beneficial to the flow of culture solution and the adhesion growth of the adherent cells, and a DISPS carrier sheet carrier which is processed by adopting a polyester fiber material suitable for the cell adhesion growth and through ultrasonic treatment is arranged between the first baffle plate 9 and the second baffle plate 22, the sheet carrier provides sufficient surface area for the cell growth, the sheet carrier can be recycled, the production efficiency is improved, and the production cost is reduced;

the two groups of connecting columns 23 are respectively connected with a PH electrode and a temperature probe, the capacitance sensor 214, the PH electrode and the temperature probe are all connected with a display, the cell concentration data, the PH concentration data and the temperature data are respectively collected through the capacitance sensor 214, the PH electrode and the temperature probe and are transmitted to the display for display, so that people can conveniently control different cultivation stages in the cell cultivation process, the model of the temperature probe can be WZP-187-3PBO, the model of the PH electrode can be E-301-CF, and the model of the capacitance sensor 214 can be EVO465;

one side of the circulating pipe 6 is horizontally connected with a return pipe 14, the return pipe 14 is semicircular, the return pipe 14 is vertically connected with a plurality of groups of first valves 4 and second valves 5, the first valves 4 are positioned above the second valves 5, one side of the plurality of groups of first valves 4, which is far away from the fermentation tank body 10, is respectively connected with a culture solution pipe, a carbon dioxide pipe, an alkali liquor pipe, a steam pipe, a sterile air pipe and a cooling water pipe, and different pipelines are used for conveying different substances, so that the required substances are conveyed only for cell culture each time, the probability of confusion among the materials is reduced, the pre-experiment of single variables on the cell culture is changed, and the cell culture process is optimized;

the stirring rod 13 is bar-shaped, the two ends of the stirring rod 13 are polished, the surface is smooth and has no edges and corners, the upper end of the downward-extending shaft body 25 is in threaded connection with the lower end of the stirring shaft 20, the downward-extending shaft body 25 is connected with a driving motor, the driving motor is started, the model of the driving motor can be 80BL110S50-430TKA, the downward-extending shaft body 25 drives the stirring shaft 20 and the stirring rod 13 to synchronously rotate, a plurality of groups of stirring blades 19 stir the flaky carrier to uniformly mix components in the fermentation tank, and the shearing action of the stirring blades 19 on adherent cells is reduced.

Various aspects disclosed in the embodiments of the present invention: the fixed ring 7, the first conduit, the conveying pipe 11, the stirring rod 13, the stirring shaft 20, the plurality of groups of stirring blades 19 and the downward extending shaft body 25 are matched for use, so that the shearing action of the stirring device on cells in the traditional scheme is reduced, various components in the fermentation tank are uniformly mixed, cell culture is facilitated, the sheet carrier provides a sufficient surface area for the growth of the cells, the sheet carrier can be recycled, the production efficiency is improved, the production cost is reduced, and the collection of cell concentration data is carried out through the capacitance sensor 214, so that people can conveniently control different culture stages in the cell culture process;

one side of the first valve 4 far away from the fermentation tank body 10 is respectively connected with a culture solution pipe, a carbon dioxide pipe, an alkali liquor pipe, a steam pipe, a sterile air pipe and a cooling water pipe, and different pipelines are used for conveying different substances, so that the condition that only the required substances are conveyed each time for cell culture is ensured, the probability of confusion among the materials is reduced, in addition, the pre-experiment of the single variable on the cell culture is facilitated, and the cell culture process is optimized.

The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention, and the invention is limited only by the claims and their full scope and equivalents.

Claims

1. The adherent cell culture fermentation tank is characterized by comprising a fermentation tank protection shell (8), a fermentation tank body (10) and a cell counting mechanism (21), wherein the cell counting mechanism (21) is positioned at a position, close to the middle, of the front end of the fermentation tank protection shell (8), the cell counting mechanism (21) penetrates through a cavity to the interior of the fermentation tank body (10), and the fermentation tank body (10) is positioned in the fermentation tank protection shell (8);

the fermentation tank comprises a fermentation tank body (10) and a fermentation tank protection shell (8), a cavity is arranged between the fermentation tank body (10) and the fermentation tank protection shell (8), the cell counting mechanism (21) comprises a bottom plate (211), a baffle plate (212), a sleeve (213) and a capacitance sensor (214), the baffle plate (212) is positioned between the bottom plate (211) and the sleeve (213), the capacitance sensor (214) penetrates through the bottom plate (211), the baffle plate (212) and the sleeve (213), a fermentation tank cover plate (12) and a rubber sealing ring are arranged at the upper end of the fermentation tank body (10), the rubber sealing ring is positioned between the fermentation tank body (10) and the fermentation tank cover plate (12), a fixed block (3) is arranged at the position of the cavity, which is close to the upper end, a lifting seat (2) and a circulating pipe (6) are arranged at one side of the fermentation tank protection shell (8), a lighting shell (16) is connected to the other side, which is close to the upper end, of the fermentation tank protection shell (8), symmetrical support legs are arranged at the lower end of the fermentation tank protection shell (8), a plurality of groups of support legs, two groups of support columns (23) are connected to the two groups of support columns (23) and a plurality of support columns (23) which are connected to each other, the lower end of the fermentation tank protection shell (8) is provided with a downward extending shaft body (25) close to the middle, the sampling column (24) and the downward extending shaft body (25) are positioned between the two groups of connecting columns (23), the sampling column (24) is positioned at one side of the downward extending shaft body (25), and the sampling column (24) and the two groups of connecting columns (23) penetrate through the cavity to the interior of the fermentation tank body (10);

two groups of conveying pipes (11) are symmetrically arranged at positions, close to two sides, of the outer surface of the fermentation tank body (10), the upper end and the lower end of each conveying pipe (11) penetrate through the interior of the fermentation tank body (10), a plurality of fixing rings (7) are symmetrically arranged at positions, close to the middle, of the interior of the fermentation tank body (10), an observation mirror (15) is arranged at positions, close to the upper end, of the front end of the fermentation tank protection shell (8), the observation mirror (15) penetrates through a cavity to the interior of the fermentation tank body (10), a stirring shaft (20) is arranged at positions, close to the middle, of the interior of the fermentation tank body (10), a connecting rod (17) is connected to the upper end of each stirring shaft (20), stirring rods (13) are welded at the upper ends of the connecting rods (17), a first partition plate (9) is connected to the position, close to the lower end of each stirring shaft (20), a second partition plate (22) is connected to the position, a plurality of groups of stirring blades (19) are arranged on the outer surface of the fermentation shaft (20), stirring blades (19) penetrate through the cavity to the interior of the fermentation tank body (10), stirring shafts (20) are arranged between the first partition plate (22) and the first partition plate (22), and the second partition plate (22) are arranged between the stirring shafts (20) and the stirring rods (20).

One side of the circulating pipe (6) is horizontally connected with a return pipe (14), the return pipe (14) is semicircular, the return pipe (14) is vertically connected with a plurality of groups of first valves (4) and second valves (5), and the first valves (4) are positioned above the second valves (5);

reinforcing block (1) is installed to one side of fermentation cylinder apron (12), the inside of fermentation cylinder apron (12) is provided with a plurality of screw, fermentation cylinder apron (12) pass through the bolt and link to each other with fixed block (3), two sets of spliced pole (23) and sampling column (24) all run through the inside of cavity to fermentation cylinder body (10), the lower extreme of sampling column (24) is connected with sample bottle (18).

2. An adherent cell culture fermenter according to claim 1, wherein one side of the first valve (4) is connected with a first conduit, the interior of the illumination housing (16) is provided with an illumination lamp, one side of the illumination housing (16) adjacent to the fermenter body (10) is connected with a second conduit, and both the first conduit and the second conduit penetrate the chamber to the interior of the fermenter body (10).

3. The adherent cell culture fermentation tank according to claim 1, wherein screw holes are formed in the outer surfaces of the connecting rod (17) and the stirring shaft (20), the connecting rod (17) and the stirring shaft (20) are connected through bolts, sealing rings are connected to the outer surfaces of the first partition plate (9) and the second partition plate (22), sieve holes are formed in the first partition plate (9) and the second partition plate (22), and a plurality of hollow casing strings are arranged on the upper surface of the first partition plate (9) close to the two sides.

4. The adherent cell culture fermenter according to claim 1, wherein a first piston rod and a second piston rod are connected above the lifting seat (2), the first piston rod is located above the second piston rod, a cylindrical through hole is formed in the position, close to the middle, of the reinforcing block (1), the first piston rod is inserted into the cylindrical through hole, and the upper surface of the first piston rod and the upper surface of the reinforcing block (1) are located on the same horizontal plane.