CN112574881A - Intestinal lymphocyte culture equipment - Google Patents
Intestinal lymphocyte culture equipment Download PDFInfo
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- CN112574881A CN112574881A CN202011457566.9A CN202011457566A CN112574881A CN 112574881 A CN112574881 A CN 112574881A CN 202011457566 A CN202011457566 A CN 202011457566A CN 112574881 A CN112574881 A CN 112574881A
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- 210000004698 lymphocyte Anatomy 0.000 title claims abstract description 72
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 61
- 238000003756 stirring Methods 0.000 claims abstract description 34
- 238000005070 sampling Methods 0.000 claims description 41
- 238000007789 sealing Methods 0.000 claims description 25
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 7
- 238000009825 accumulation Methods 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 3
- 238000004113 cell culture Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 230000005484 gravity Effects 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 3
- 230000009545 invasion Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 101150114468 TUB1 gene Proteins 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The invention relates to the technical field of cell culture, in particular to intestinal lymphocyte culture equipment; comprises a barrel body, a covering mechanism, a stirring mechanism and a driving mechanism. The bottom of the barrel body is provided with a rotating groove, the bottom of the barrel body is provided with a cavity, and the cavity is communicated with the rotating groove; the covering mechanism is detachably and hermetically sleeved at the top of the barrel body; the stirring mechanism comprises a rotating part and a stirring rod, the rotating part is rotatably and hermetically arranged in the rotating groove, the stirring rod is positioned in the barrel, and the bottom of the stirring rod is fixedly arranged at the top of the rotating part; the driving mechanism is arranged in the cavity, one end part of the driving mechanism is fixedly sleeved on the rotating piece, and the driving mechanism is used for driving the rotating piece to rotate. The invention aims to solve the problem that in the process of culturing intestinal lymphocytes by using the conventional culture device, workers cannot shake the intestinal lymphocytes uniformly.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to intestinal lymphocyte culture equipment.
Background
The intestinal tract is the most complex mucosal immune organ of the body, is an important organ for identifying self and abnormal self, and is also a main door for pathogen invasion, so that the intestinal tract mucosal immune system forms the first immune barrier for resisting pathogen invasion.
The mucosal immune system includes lymphocyte, T cell, K cell immunity, etc. Among them, the lymphocytes in the intestinal tract are the largest lymphocyte population in vivo. The main function of lymphocytes is cytolytic activity, a mechanism by which the host responds rapidly to pathogen invasion. I.e., lymphocytes, are the first line "soldiers" to fight external infection and monitor cellular variation in vivo.
At present, when intestinal lymphocytes are cultured, devices such as culture bottles and culture dishes are often used for sealed culture so as to prevent the intestinal lymphocytes cultured in the culture bottles from being polluted.
When the staff is culturing the intestinal lymphocyte, the intestinal lymphocyte in the culture bottle needs to be shaken up, and meanwhile, part of the lymphocyte in the culture bottle also needs to be taken out for sampling and detection. However, when the culture device is in a sealed state, the worker needs to open the cover to remove the intestinal lymphocytes in the culture device, so that the intestinal lymphocytes in the culture device are contaminated. Meanwhile, the staff can not shake the intestinal tract lymphocytes by shaking the bottle body.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides an intestinal lymphocyte culture device, which aims to solve the problem that in the process of culturing intestinal lymphocytes by using the conventional culture device, workers cannot shake the intestinal lymphocytes uniformly.
In order to achieve the purpose, the invention adopts the following technical scheme: an intestinal lymphocyte culture apparatus, comprising:
the bottom of the barrel body is provided with a rotating groove, and a cavity is arranged at the bottom of the barrel body and is communicated with the rotating groove;
the covering mechanism is detachably sleeved on the top of the barrel body in a sealing manner;
the stirring mechanism comprises a rotating part and a stirring rod, the rotating part is rotatably and hermetically arranged in the rotating groove, the stirring rod is positioned in the barrel, and the bottom of the stirring rod is fixedly arranged at the top of the rotating part; and
and the driving mechanism is arranged in the cavity, one end part of the driving mechanism is fixedly sleeved on the rotating part, and the driving mechanism is used for driving the rotating part to rotate.
When adopting above-mentioned technical scheme, when intestinal lymphocyte cultivates in the staving, the lid closes the mechanism lid at the top of staving, seals the staving, when needs shake the even intestinal lymphocyte in the staving, starts actuating mechanism, and the rotation is rotated in the actuating mechanism drive to drive the puddler and rotate, thereby shake the even to intestinal lymphocyte through the puddler. Therefore, the intestinal lymphocytes in the barrel body can be quickly shaken up better by the working personnel.
Preferably, the closing mechanism comprises a cover and a handle, the cover is rotatably and threadedly mounted at the top of the barrel body in a sealing manner, and the handle is fixedly mounted at the top of the cover.
The cover is rotated, so that the barrel body can be opened or closed conveniently, meanwhile, the lifting handle is lifted, and workers can carry the cover or the barrel body conveniently.
Preferably, the rotating part comprises a rotating barrel and a rotating disc, the rotating barrel is rotatably installed in the rotating groove, the top of the rotating barrel extends out of the rotating groove, the rotating disc is rotatably and hermetically installed at the bottom of the barrel body, the rotating disc is fixedly sleeved on the rotating barrel, and the stirring rod is fixedly installed at the top of the rotating disc.
Actuating mechanism drives and rotates the bucket and rotate, and then drives the rolling disc and rotate to realize the rotation of puddler, and then stir intestinal lymphocyte through the puddler.
Preferably, the rotating member further comprises a sealing plate, a torsion spring and a pull rope, an opening communicated with the outside is formed in one side wall of the rotating barrel, the sealing plate can be opened or closed to the barrel opening of the rotating barrel, two ends of the torsion spring are respectively fixedly connected with the rotating disc and the end portion of the sealing plate, one end of the pull rope is fixedly installed at the bottom of the sealing plate far away from one end of the torsion spring, the other end of the pull rope can be tensioned or loosened in the rotating barrel, and the pull rope can pull the sealing plate to close the barrel opening of the rotating barrel in a force storage state of the torsion spring.
The pulling stay cord to realize that the closing plate seals the rotating barrel, when needs examine the intestinal lymphocyte in the staving, loosen the stay cord, the torsional spring drives the closing plate and opens the bung hole that rotates the bucket, makes the intestinal lymphocyte flow in to rotate the bucket, takes out the intestinal lymphocyte in the rotating barrel, thereby realizes being convenient for detect the intestinal lymphocyte.
Preferably, the sampler also comprises a sampling mechanism, wherein the sampling mechanism comprises a bottom plate, a supporting spring, a sampling barrel, a limiting rod and a limiting spring; the bottom of the barrel body is provided with a stepped hole communicated with the rotating groove, the bottom plate is slidably and hermetically arranged in the rotating barrel along the height direction of the rotating barrel, the bottom plate is fixedly connected with one end of the pull rope, which is far away from the sealing plate, and the bottom plate is provided with a limiting groove with a notch opposite to the stepped hole; the two ends of the supporting spring are fixedly arranged at the bottom of the bottom plate and the bottom of the rotating barrel respectively, the sampling barrel is detachably arranged on the bottom plate and can slide along the height direction of the rotating barrel and be taken out from the opening, the side wall of the sampling barrel is provided with a sliding groove, and a rope body of the pull rope is sealed in the sliding groove in a sliding manner; the limiting rod is slidably inserted in the stepped hole in a sealing manner, the limiting rod can slide into or slide out of the limiting groove, the limiting spring is located in the flaring of the stepped hole, the limiting spring is sleeved on the limiting rod, and two ends of the limiting spring are fixedly connected with the limiting rod and the hole wall of the stepped hole respectively.
When needs are sampled intestinal lymphocyte, the gag lever post of outwards sliding, make spacing spring hold power, supporting spring supporting baseplate and sampling bucket upward movement, make the stay cord crooked, the torsional spring and then drives the closing plate and rotate, thereby make intestinal lymphocyte flow in the sampling bucket, gravity in the sampling bucket increases, the extrusion supporting spring, thereby make bottom plate and sampling bucket downstream, drive the stay cord simultaneously and reset, thereby it lives the rotation bucket to drive the closing plate seal, reinsert the gag lever post, take out the sampling bucket, thereby the staff of being convenient for takes a sample.
Preferably, the driving mechanism comprises a motor, a first bevel gear and a second bevel gear, the motor is fixedly installed in the cavity, the first bevel gear is fixedly installed on a rotating shaft of the motor, the second bevel gear is fixedly installed on the rotating barrel in a fixed sleeved mode, and the second bevel gear is meshed with the first bevel gear.
And starting the motor, wherein the motor drives the first bevel gear to rotate, and the first bevel gear drives the second bevel gear to rotate, so that the rotating barrel is driven to rotate through the second bevel gear.
Preferably, the bottom of the barrel body is fixedly provided with an anti-skid seat.
The anti-skidding seat is convenient for carry out the antiskid to the staving.
The beneficial effect that this scheme produced is:
1. through the arrangement of the rotating piece, the stirring rod and the driving mechanism. After the driving mechanism is started, the driving mechanism drives the rotating part to rotate, so that the stirring rod is driven to rotate, and intestinal lymphocytes are shaken up through the stirring rod. Therefore, the intestinal lymphocytes in the barrel body can be quickly shaken up better by the working personnel.
2. Through the setting of closing plate, torsional spring and stay cord, the closing plate seals the rotating barrel, simultaneously, loosens the stay cord, and the torsional spring drives the closing plate and opens the bung hole that rotates the barrel for intestinal lymphocyte in the barrel falls into the rotating barrel in, thereby realizes collecting the intestinal lymphocyte that needs detected.
Drawings
In order to more clearly illustrate the embodiments of the present invention, the drawings, which are required to be used in the embodiments, will be briefly described below. In all the drawings, the elements or parts are not necessarily drawn to actual scale.
FIG. 1 is a front view of an intestinal lymphocyte culture apparatus according to the present invention;
FIG. 2 is a schematic view in the direction A-A of FIG. 1;
FIG. 3 is a schematic view in the direction B-B in FIG. 1;
FIG. 4 is a top view of the intestinal lymphocyte culture apparatus at a position of a limiting rod;
FIG. 5 is a top view of an intestinal lymphocyte culture apparatus according to the present invention.
Reference numerals:
1-barrel body, 11-chamber, 12-antiskid seat;
2-a covering mechanism, 21-a cover and 22-a carrying handle;
3-stirring mechanism, 31-rotating part, 311-rotating barrel, 312-rotating disk, 313-sealing plate, 314-torsion spring, 315-pull rope, 316-opening and 32-stirring rod;
4-driving mechanism, 41-motor, 42-first bevel gear, 43-second bevel gear;
5-sampling mechanism, 51-bottom plate, 52-supporting spring, 53-sampling barrel, 54-limiting rod and 55-limiting spring.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
Referring to fig. 1-5, an intestinal lymphocyte culture apparatus includes a barrel 1, a covering mechanism 2, a stirring mechanism 3 and a driving mechanism 4.
The middle of the bottom of the barrel body 1 is provided with a rotating groove, the notch of the rotating groove faces the opening of the barrel body 1, a cavity 11 is arranged inside the bottom of the barrel body 1, and the cavity 11 is communicated with the rotating groove. Wherein, the bottom of the barrel body 1 is fixedly provided with an anti-skid seat 12. In this embodiment, the anti-slip seats 12 are four, and four anti-slip seats 12 are uniformly distributed and fixedly installed at the bottom of the barrel body 1.
The covering mechanism 2 is detachably and hermetically sleeved at the top of the barrel body 1. Specifically, the closing mechanism 2 comprises a cover 21 and a handle 22, the cover 21 is rotatably and threadedly mounted on the top of the barrel body 1 in a sealing manner, and the handle 22 is fixedly mounted on the top of the cover 21. The lid 21 is rotated to facilitate the opening or closing of the tub 1, and at the same time, the handle 22 is lifted to facilitate the worker's carrying of the lid 21 or the tub 1.
Referring to fig. 2, the stirring mechanism 3 includes a rotating member 31 and a stirring rod 32, the rotating member 31 is rotatably and hermetically installed in the rotating tank, the stirring rod 32 is located in the barrel 1, and the bottom of the stirring rod 32 is fixedly installed at the top of the rotating member.
Specifically, the rotating member 31 includes a rotating barrel 311 and a rotating disc 312, the rotating barrel 311 is rotatably installed in the rotating groove, the top of the rotating barrel 311 extends out of the rotating groove, the rotating disc 312 is rotatably and hermetically installed at the bottom of the barrel body 1, the rotating disc 312 is fixedly sleeved on the rotating barrel 311, and the top of the rotating disc 312 is fixedly provided with the stirring rod 32. The driving mechanism 4 drives the rotating barrel 311 to rotate, and then drives the rotating disc 312 to rotate, so as to realize the rotation of the stirring rod 32, and further stir the intestinal tract lymphocytes through the stirring rod 32.
Referring to fig. 3, the driving mechanism 4 is installed in the chamber 11, one end of the driving mechanism 4 is fixedly sleeved on the rotating member 31, and the driving mechanism 4 is used for driving the rotating member 31 to rotate. Specifically, the driving mechanism 4 includes a motor 41, a first bevel gear 42 and a second bevel gear 43, the motor 41 is fixedly installed in the chamber 11, the first bevel gear 42 is fixedly installed on a rotating shaft of the motor 41, the second bevel gear 43 is fixedly sleeved on the rotating barrel 311, and the second bevel gear 43 is engaged with the first bevel gear 42. The motor 41 is started, the motor 41 drives the first bevel gear 42 to rotate, the first bevel gear 42 drives the second bevel gear 43 to rotate, and thereby the rotating barrel 311 is driven to rotate by the second bevel gear 43.
In order to facilitate the detection of intestinal lymphocytes by the staff, in this embodiment, please refer to fig. 2 together, the rotating member 31 further includes a sealing plate 313, a torsion spring 314, and a pulling rope 315, an opening 316 communicating with the outside is disposed on a side wall of the rotating barrel 311, the sealing plate 313 can open or close the opening of the rotating barrel 311, two ends of the torsion spring 314 are fixedly connected to the end portions of the rotating disc 312 and the sealing plate 313, one end of the pulling rope 315 is fixedly mounted at the bottom of the sealing plate 313 far away from the end of the torsion spring 314, the other end of the pulling rope 315 can be tensioned or loosened in the rotating barrel 311, and the pulling rope 315 pulls the sealing plate 313 to close the opening of the rotating barrel 311 in the state of. Pulling stay cord 315, thereby realize that closing plate 313 seals rotating barrel 311, when the intestinal lymphocyte that needs in to staving 1 detects, pass opening 316, put into rotating barrel 311 sampling devices such as sampling bucket 53 in, loosen stay cord 315, torsional spring 314 drives closing plate 313 and opens the bung hole of rotating barrel 311, make the intestinal lymphocyte flow in sampling bucket 53, take out the intestinal lymphocyte in the sampling bucket 53, thereby realize being convenient for detecting the intestinal lymphocyte.
In order to be able to better sample the cultured lymphocytes. Referring to fig. 2 and 3, the intestinal lymphocyte culture apparatus further includes a sampling mechanism 5, wherein the sampling mechanism 5 includes a bottom plate 51, a support spring 52, a sampling barrel 53, a limiting rod 54 and a limiting spring 55.
Wherein, the bottom of staving 1 is equipped with the shoulder hole that the intercommunication rotated the groove, and bottom plate 51 is along rotating the slidable seal installation of the direction of height of bucket 311 in rotating bucket 311, and bottom plate 51 and the bottom fixed connection of stay cord 315, bottom plate 51 are equipped with the notch and can be with the relative spacing groove of shoulder hole. The both ends of supporting spring 52 are fixed mounting respectively in the bottom of bottom plate 51 and the bottom of rotating bucket 311, and sample bucket 53 passes through the mode detachable of bonding to install on bottom plate 51 to put into or take out bottom plate 51 from the opening sample bucket 53 on, and can slide along the direction of height that rotates bucket 311, and the lateral wall of sample bucket 53 is equipped with the sliding tray, and sliding seal has the rope body of stay cord 315 in the sliding tray.
Specifically, the limiting rod 54 can be slidably inserted in the stepped hole in a sealing manner, the limiting rod 54 can slide into or out of the limiting groove, the limiting spring 55 is located in an expansion opening of the stepped hole, the limiting spring 55 is sleeved on the limiting rod 54, and two ends of the limiting spring 55 are fixedly connected with the limiting rod 54 and the hole wall of the stepped hole respectively. When the needs are sampled intestinal lymphocyte, outside slip gag lever post 54, make spacing spring 55 hold power, supporting spring 52 supports bottom plate 51 and sampling bucket 53 upward movement, make stay cord 315 crooked, torsional spring 314 and then drive closing plate 313 and rotate, thereby make intestinal lymphocyte flow in sampling bucket 53, gravity in the sampling bucket 53 increases, extrusion supporting spring 52, thereby make bottom plate 51 and sampling bucket 53 downstream, it resets to drive stay cord 315 simultaneously, thereby drive closing plate 313 and seal rotation bucket 311, reinsert gag lever post 54, take out sampling bucket 53, thereby be convenient for the staff to take a sample.
When the needs are sampled intestinal lymphocyte, outside slip gag lever post 54, make spacing spring 55 hold power, supporting spring 52 supports bottom plate 51 and sampling bucket 53 upward movement, make stay cord 315 crooked, torsional spring 314 and then drive closing plate 313 and rotate, thereby make intestinal lymphocyte flow in sampling bucket 53, gravity in the sampling bucket 53 increases, extrusion supporting spring 52, thereby make bottom plate 51 and sampling bucket 53 downstream, it resets to drive stay cord 315 simultaneously, thereby drive closing plate 313 and seal rotation bucket 311, reinsert gag lever post 54, take out sampling bucket 53, thereby be convenient for the staff to take a sample.
When the intestinal lymphocyte culture device is used, when intestinal lymphocytes are cultured in the barrel body 1, the carrying handle 22 is held, the cover 21 is rotated, and then the cover 21 is sealed on the top of the barrel body 1 to seal the barrel body 1. When intestinal tract lymphocytes in the barrel body 1 need to be shaken up, the motor 41 is started, the motor 41 drives the first bevel gear 42 to rotate, the first bevel gear 42 drives the second bevel gear 43 to rotate, and therefore the rotating barrel 311 is driven to rotate through the second bevel gear 43; so that the rotating disc 312 and the stirring rod 32 rotate together, thereby realizing shaking up of intestinal lymphocytes in the barrel body 1.
When the intestinal lymphocyte in the staving 1 needs to be sampled and detected, the gag lever post 54 slides outwards, make spacing spring 55 hold up power, supporting spring 52 supporting baseplate 51 and sampling bucket 53 upward movement, torsional spring 314 and then drive closing plate 313 and rotate, thereby make the intestinal lymphocyte flow in sampling bucket 53, along with the increase of gravity in the sampling bucket 53, sampling bucket 53 extrudes supporting spring 52, thereby make baseplate 51 and sampling bucket 53 move down, drive stay cord 315 to reset simultaneously, thereby drive closing plate 313 and seal rotating barrel 311, reinsert gag lever post 54, take out sampling bucket 53 in the opening 316, thereby realize that the staff samples the intestinal lymphocyte.
In the above technical solution, the rotor 31, the stirring rod 32 and the driving mechanism 4 are provided. After the driving mechanism 4 is started, the driving mechanism 4 drives the rotating part 31 to rotate, so as to drive the stirring rod 32 to rotate, and accordingly intestinal lymphocytes are shaken up through the stirring rod 32. Therefore, the intestinal lymphocytes in the barrel body 1 can be quickly shaken up better by the working personnel.
Through the setting of closing plate 313, torsional spring 314 and stay cord 315, closing plate 313 seals rotating barrel 311, simultaneously, loosens stay cord 315, and torsional spring 314 drives closing plate 313 and opens rotating barrel 311's bung hole for the intestinal lymphocyte in the staving 1 falls into rotating barrel 311, thereby realizes collecting the intestinal lymphocyte that needs detected.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (7)
1. An intestinal lymphocyte culture apparatus, comprising:
the bottom of the barrel body is provided with a rotating groove, and a cavity is arranged at the bottom of the barrel body and is communicated with the rotating groove;
the covering mechanism is detachably sleeved on the top of the barrel body in a sealing manner;
the stirring mechanism comprises a rotating part and a stirring rod, the rotating part is rotatably and hermetically arranged in the rotating groove, the stirring rod is positioned in the barrel, and the bottom of the stirring rod is fixedly arranged at the top of the rotating part; and
and the driving mechanism is arranged in the cavity, one end part of the driving mechanism is fixedly sleeved on the rotating part, and the driving mechanism is used for driving the rotating part to rotate.
2. The intestinal lymphocyte culture apparatus according to claim 1, wherein the closure mechanism comprises a lid and a handle, the lid is rotatably screwed and sealed on the top of the barrel, and the handle is fixedly mounted on the top of the lid.
3. The intestinal lymphocyte culture device according to claim 2, wherein the rotating member comprises a rotating barrel and a rotating disc, the rotating barrel is rotatably mounted in the rotating groove, the top of the rotating barrel extends out of the rotating groove, the rotating disc is rotatably and hermetically mounted at the bottom of the barrel body, the rotating disc is fixedly mounted on the rotating barrel, and the stirring rod is fixedly mounted at the top of the rotating disc.
4. The intestinal tract lymphocyte culture equipment according to claim 3, wherein the rotating member further comprises a sealing plate, a torsion spring and a pull rope, an opening communicated with the outside is formed in one side wall of the rotating barrel, the sealing plate can open or close the opening of the rotating barrel, two ends of the torsion spring are respectively fixedly connected with the rotating disc and the end portion of the sealing plate, one end of the pull rope is fixedly installed at the bottom of one end, away from the torsion spring, of the sealing plate, the other end of the pull rope can be tensioned or loosened in the rotating barrel, and the pull rope can pull the sealing plate to close the opening of the rotating barrel in the force accumulation state of the torsion spring.
5. The intestinal lymphocyte culture equipment according to claim 4, further comprising a sampling mechanism, wherein the sampling mechanism comprises a bottom plate, a support spring, a sampling barrel, a limiting rod and a limiting spring;
the bottom of the barrel body is provided with a stepped hole communicated with the rotating groove, the bottom plate is slidably and hermetically arranged in the rotating barrel along the height direction of the rotating barrel, the bottom plate is fixedly connected with one end of the pull rope, which is far away from the sealing plate, and the bottom plate is provided with a limiting groove with a notch opposite to the stepped hole;
the two ends of the supporting spring are fixedly arranged at the bottom of the bottom plate and the bottom of the rotating barrel respectively, the sampling barrel is detachably arranged on the bottom plate and can slide along the height direction of the rotating barrel and be taken out from the opening, the side wall of the sampling barrel is provided with a sliding groove, and a rope body of the pull rope is sealed in the sliding groove in a sliding manner;
the limiting rod is slidably inserted in the stepped hole in a sealing manner, the limiting rod can slide into or slide out of the limiting groove, the limiting spring is located in the flaring of the stepped hole, the limiting spring is sleeved on the limiting rod, and two ends of the limiting spring are fixedly connected with the limiting rod and the hole wall of the stepped hole respectively.
6. The intestinal lymphocyte culture device according to claim 1 or 5, wherein the driving mechanism comprises a motor, a first bevel gear and a second bevel gear, the motor is fixedly installed in the chamber, the first bevel gear is fixedly installed on a rotating shaft of the motor, the second bevel gear is fixedly installed on the rotating barrel, and the second bevel gear is meshed with the first bevel gear.
7. The intestinal lymphocyte culture device according to claim 6, wherein the bottom of the barrel body is fixedly provided with an anti-slip seat.
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| CN202011457566.9A CN112574881A (en) | 2020-12-10 | 2020-12-10 | Intestinal lymphocyte culture equipment |
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| CN202011457566.9A CN112574881A (en) | 2020-12-10 | 2020-12-10 | Intestinal lymphocyte culture equipment |
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| CN112574881A true CN112574881A (en) | 2021-03-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113384060A (en) * | 2021-05-12 | 2021-09-14 | 中国人民解放军陆军军医大学 | Make-up spray gun |
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| CN113384060B (en) * | 2021-05-12 | 2022-07-05 | 中国人民解放军陆军军医大学 | Make-up spray gun |
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