CN204644370U - Cell suspension culture device - Google Patents
Cell suspension culture device Download PDFInfo
- Publication number
- CN204644370U CN204644370U CN201520334764.4U CN201520334764U CN204644370U CN 204644370 U CN204644370 U CN 204644370U CN 201520334764 U CN201520334764 U CN 201520334764U CN 204644370 U CN204644370 U CN 204644370U
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- cup
- cell
- nutrient solution
- paddle
- suspension culture
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Abstract
A kind of cell suspension culture device, comprises base, cultivates cup.Cultivating cup is arranged on base, be provided with stirrer in base, the rotor that described stirrer is arranged is positioned at the bottom of cultivating cup, and described rotor is coaxially installed with fixed link, described fixed link sidewall is provided with multiple paddle, and described paddle is up-narrow and down-wide right-angled trapezium.Cell suspension culture device makes the nutrient solution in cultivation cup be in flow state by stirrer always, avoid nutrient solution static, the cell grown all is suspended in nutrient solution, avoid it to paste on the wall, prevent the cultivation amount of wall of container area constraints cell, thus increase the cell total amount of turning out, make it can meet the requirement of production in enormous quantities, the cell of turning out can be communicated with nutrient solution and pour out in the lump, and the taking-up of cell is easy, is not easy the destruction of causing cell.The fixed link that rotor is installed and paddle form a stirring rake, stirring rake can cover the short transverse of whole cultivation cup, paddle is up-narrow and down-wide, make the large position of the large pressure of medium depth can be subject to more whipping force, ensure that the nutrient solution of each position in whole cultivation cup is all sufficiently agitated, improve mixing effect, avoid the cellular retention of turning out on cultivation cup wall of cup.
Description
Technical field
The utility model relates to biomedicine field, specifically, is a kind of cell suspension culture device.
Background technology
In biological medicine is produced, in the production of particularly vaccine, need to cultivate the inoculation that some cells carry out virus, and the cultivation of cell generally adopts culturing bottle or rolling bottle.When utilizing culturing bottle or rolling bottle to carry out cell cultures, cell is all long on bottle inwall, be limited to the area of bottle inwall, the cell total amount of turning out have received the restriction of area, output is little, be not suitable for the requirement of producing in enormous quantities, and cell is taken out from culturing bottle also comparatively bother, also easily destroy cell.
Utility model content
The deficiency that the utility model exists for above-mentioned prior art, devises a kind of cell suspension culture device.
Cell suspension culture device of the present utility model, comprises base, cultivates cup.
Described cultivation cup is arranged on base, and described cultivation cup sidewall is provided with adding mouth and thief hole, and described adding mouth and thief hole are positioned at sustained height position, and the rim of a cup of described cultivation cup, adding mouth and thief hole are all provided with sealing cover.
Be provided with stirrer in described base, the rotor that described stirrer is arranged is positioned at the bottom of cultivating cup, and described rotor is coaxially installed with fixed link, described fixed link sidewall is provided with multiple paddle, described paddle is up-narrow and down-wide right-angled trapezium.
Preferably, described adding mouth and thief hole are all inclined upwardly.
Preferably, described sealing cover is all threaded connection with corresponding opening.
The beneficial effects of the utility model are: cell suspension culture device makes the nutrient solution in cultivation cup be in flow state by stirrer always, avoid nutrient solution static, the cell grown all is suspended in nutrient solution, it is avoided to paste on the wall, prevent the cultivation amount of wall of container area constraints cell, thus increase the cell total amount of turning out, make it can meet the requirement of production in enormous quantities, the cell of turning out can be communicated with nutrient solution and pour out in the lump, the taking-up of cell is easy, is not easy the destruction of causing cell.The fixed link that rotor is installed and paddle form a stirring rake, stirring rake can cover the short transverse of whole cultivation cup, paddle is up-narrow and down-wide, make the large position of the large pressure of medium depth can be subject to more whipping force, ensure that the nutrient solution of each position in whole cultivation cup is all sufficiently agitated, improve mixing effect, avoid the cellular retention of turning out on cultivation cup wall of cup.Adding mouth and the thief hole of cultivating cup setting can be convenient to adding of nutrient solution and sample detect current cultivation conditions, and adding mouth and thief hole are positioned at sustained height position, and avoiding highly differs makes nutrient solution spill from one of them mouth.Opening is all provided with sealing cover, and avoid external substance to cause nutrient solution to pollute, and adding mouth and thief hole are all inclined upwardly, and nutrient solution also can be avoided to spill, preventing pollution thing enters.Sealing cover is threaded connection, and improves sealing effectiveness.
Accompanying drawing explanation
Accompanying drawing 1 is the structural representation of cell suspension culture device.
Embodiment
Cell suspension culture device of the present utility model, as shown in Figure 1, comprises base 1, cultivates cup 2.
Cultivating cup 2 is arranged on base 1, and cultivate on cup 2 sidewall and be provided with adding mouth 3 and thief hole 4, adding mouth 3 and thief hole 4 are positioned at sustained height position, and adding mouth 3 and thief hole 4 are all inclined upwardly.
Cultivate on the rim of a cup of cup 2, adding mouth 3 and thief hole 4 and be all provided with sealing cover 5, sealing cover 5 is all threaded connection with corresponding opening.
Be provided with stirrer in base 1, the rotor 6 that stirrer is arranged is positioned at the bottom of cultivating cup 2.Base 1 also can be installed the temperature control heating device that can be and cultivate cup 2 and heat.
Rotor 6 is coaxially installed with fixed link 7, fixed link 7 sidewall is provided with multiple paddle 8, paddle 8 and fixed link 7 form a stirring rake be arranged on rotor 6.Paddle 8 is up-narrow and down-wide right-angled trapezium.
During use, open the sealing cover 5 cultivating cup 2 rim of a cup, nutrient solution and cytomixis are poured into, cover sealing cover 5, open stirrer, the stirring rake on rotor 6 and rotor 6 is rotated, liquid in cup 2 is cultivated in prolonged agitation, and cell is bred in the nutrient solution of flowing.Open temperature control heating device, make fluid temperature in cultivation cup 2 be heated to the temperature of suitable cell cultivation, accelerate cell cultures speed.
By controlling rotating speed, the control Heating temperature of rotor 6 and stirring rake, prevent cell low temperature from not breeding or the too high death of rotating speed.
Claims (3)
1. a cell suspension culture device, is characterized in that, comprises base (1), cultivates cup (2),
Described cultivation cup (2) is arranged on base (1), described cultivation cup (2) sidewall is provided with adding mouth (3) and thief hole (4), described adding mouth (3) and thief hole (4) are positioned at sustained height position, and the rim of a cup of described cultivation cup (2), adding mouth (3) and thief hole (4) are all provided with sealing cover (5);
Described base is provided with stirrer in (1), the rotor (6) that described stirrer is arranged is positioned at the bottom of cultivating cup (2), described rotor (6) is coaxially installed with fixed link (7), described fixed link (7) sidewall is provided with multiple paddle (8), described paddle (8) is up-narrow and down-wide right-angled trapezium.
2. cell suspension culture device according to claim 1, is characterized in that, described adding mouth (3) and thief hole (4) are all inclined upwardly.
3. cell suspension culture device according to claim 1, is characterized in that, described sealing cover (5) is all threaded connection with corresponding opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520334764.4U CN204644370U (en) | 2015-05-22 | 2015-05-22 | Cell suspension culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520334764.4U CN204644370U (en) | 2015-05-22 | 2015-05-22 | Cell suspension culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204644370U true CN204644370U (en) | 2015-09-16 |
Family
ID=54097131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520334764.4U Active CN204644370U (en) | 2015-05-22 | 2015-05-22 | Cell suspension culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204644370U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107177486A (en) * | 2017-06-21 | 2017-09-19 | 广西放心源生物科技有限公司 | Streptococcus suis culture inactivation integrated bottle |
| CN112574881A (en) * | 2020-12-10 | 2021-03-30 | 中国人民解放军陆军军医大学第二附属医院 | Intestinal lymphocyte culture equipment |
-
2015
- 2015-05-22 CN CN201520334764.4U patent/CN204644370U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107177486A (en) * | 2017-06-21 | 2017-09-19 | 广西放心源生物科技有限公司 | Streptococcus suis culture inactivation integrated bottle |
| CN112574881A (en) * | 2020-12-10 | 2021-03-30 | 中国人民解放军陆军军医大学第二附属医院 | Intestinal lymphocyte culture equipment |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |