CN203855589U - Aerobic biological cell culture apparatus - Google Patents
Aerobic biological cell culture apparatus Download PDFInfo
- Publication number
- CN203855589U CN203855589U CN201420295038.1U CN201420295038U CN203855589U CN 203855589 U CN203855589 U CN 203855589U CN 201420295038 U CN201420295038 U CN 201420295038U CN 203855589 U CN203855589 U CN 203855589U
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- China
- Prior art keywords
- tank body
- mounting base
- cultivation tank
- cell culture
- culture apparatus
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Abstract
The utility model provides an aerobic biological cell culture apparatus which comprises an installation bottom plate, wherein the installation bottom plate is characterized in that the installation bottom plate is connected with a culture tank body through a group of springs, a group of arc-shaped baffle bars are arranged at the bottom of the culture tank body, a rotary disc which is arranged on the installation bottom plate is located between the upper part of the installation bottom plate and the bottom of the culture tank, the rotary disc is provided with a semispherical bump, and the semispherical bump is higher than the bottoms of the arc-shaped baffle bars but lower than the bottom of the culture tank body. By way of swinging and stirring, the device provided by the utility model is simple in swing mechanism, and the equipment is low in investment cost and convenient to maintain and popularize and apply.
Description
Technical field:
The utility model relates to a kind of aerobe cell culture apparatus, belongs to biological plant technical field.
Background technology:
By zooblast vitro culture, express and produce all kinds of diagnosis and treatment monoclonal antibody, vaccine, the biological activity proteins such as somatomedin have market application foreground very widely.Therefore, be used for large scale and high density to cultivate the biomass cells reactor of zooblast, just become most important equipment in biotechnological pharmaceutics industry.It has determined product cost, industrial scale and the product category of biotechnological pharmaceutics enterprise to a great extent.Existing reactor adopts the mechanical stirring mode of inserting in tank body to promote flowing of liquid, the problem of the microbial contamination that in work, whipping appts structure and lubricating substance thereof often bring, living contaminants stirs lubricated situation incidence of bringing miscellaneous bacteria into high, once will cause necrocytosis.
In order to address the above problem, Chinese patent CN201010160973 discloses a kind of polygonal plastic bag swing cell reactor, and specifically disclose following technical characterictic: shaking table, on described shaking table, connect wabble mechanism, Polygons retort is installed in described wabble mechanism the inside, Polygons plastic culture bag is equipped with in described Polygons retort the inside, this patent adopts the mode of stirring stirring of waving, avoided inserting the cell contamination that the mechanical stirring in tank body causes, but this wabble mechanism complex structure, cause equipment that difficulty is installed, and manufacture and maintenance cost are high, narrow application range.
Summary of the invention:
The purpose of this utility model is that the problem for above-mentioned existence provides a kind of aerobe cell culture apparatus, adopt the mode of waving stirring, and wabble mechanism is simple, and equipment investment cost is low, easy to maintenance, easy to utilize.
Above-mentioned object realizes by following technical scheme:
Aerobe cell culture apparatus, comprise mounting base, described mounting base is connected and is cultivated tank body by one group of spring, the bottom of described cultivation tank body is provided with one group of cabochon apron strip, on described mounting base, be provided with rolling disc, described rolling disc is between the top of described mounting base and the bottom of described cultivation tank body, on described rolling disc, be provided with a hemispherical projections, the height of described hemispherical projections is higher than the bottom of described cabochon apron strip and lower than the bottom of described cultivation tank body.
Described aerobe cell culture apparatus, described cultivation tank body top is provided with functional cover, described functional cover comprises the cover plate being connected with described cultivation tank body, is provided with airway, dissolved oxygen rate tester, thermometer through described cover plate on described cover plate.
Described aerobe cell culture apparatus, described mounting base middle part is provided with bearing, and described rolling disc middle part is provided with connecting rod, and described connecting rod is connected with drive-motor with step-down gear through the bearing at described mounting base middle part.
Described aerobe cell culture apparatus, the bottom, the inside of described cultivation tank body is provided with cone-shaped body, and the aspect ratio of described cultivation tank body is between 0.2-0.3.
Beneficial effect:
1. the utility model has avoided traditional insert agitator easily to pollute and shearing force is crossed the defect of havoc biomass cells, also structure simultaneously that be different from the disclosed complexity of patent CN201010160973, as long as a rotating disk is set below cultivation tank body, the bottom of simultaneously cultivating tank body is provided with cabochon apron strip, when dial rotation, when the projection on rotating disk is encountered cabochon apron strip, because the bottom of the convexity cabochon apron strip on rotating disk wants high, therefore the projection on rotating disk causes cabochon apron strip jack-up to cultivate tank body integral body by jack-up, cultivate Tank tilting, when being rotated further when driving projection to leave this cabochon apron strip, rotating disk resets under the effect of tank body at spring, when driven by rotary disc projection turns to next cabochon apron strip, cultivating tank body tilts again, and so forth, cultivate tank body and constantly rock the object that reaches stirring, equipment investment cost is low, easy to maintenance, easy to utilize.
2. if the aspect ratio of the stirring tank body of this rocking type is excessive, easily cause stirring inhomogeneous, therefore through test of many times research, find, cultivate aspect ratio mixing effect the best between 0.2-0.3 of tank body.
3. the utility model is provided with cone-shaped body in tank base, and in the process of rocking at tank body, cone-shaped body can play the effect of stirring better, and reactive force temperature, can not produce large damage to cell.
Accompanying drawing explanation:
Fig. 1 is front view of the present utility model.
Fig. 2 is the bottom surface upward view of cultivation tank body of the present utility model.
Fig. 3 is the vertical view of rolling disc of the present utility model.
In figure: 1, mounting base; 2, spring; 3, cultivate tank body; 4, cabochon apron strip; 5, rolling disc; 6, hemispherical projections; 7, cover plate; 8, airway; 9, dissolved oxygen rate tester; 10, thermometer; 11, connecting rod; 12, step-down gear; 13, drive-motor; 14, cone-shaped body.
Embodiment:
As Figure 1-3: the aerobe cell culture apparatus of the present embodiment, comprise mounting base 1, described mounting base is connected and is cultivated tank body 3 by one group of spring 2, the bottom of described cultivation tank body is provided with one group of cabochon apron strip 4, on described mounting base, be provided with rolling disc 5, described rolling disc is between the top of described mounting base and the bottom of described cultivation tank body, on described rolling disc, be provided with a hemispherical projections 6, the height of described hemispherical projections is higher than the bottom of described cabochon apron strip and lower than the bottom of described cultivation tank body.
In the present embodiment, described cultivation tank body top is provided with functional cover, and described functional cover comprises the cover plate 7 being connected with described cultivation tank body, is provided with airway 8, dissolved oxygen rate tester 9, thermometer 10 through described cover plate on described cover plate.
In the present embodiment, described mounting base middle part is provided with bearing, and described rolling disc middle part is provided with connecting rod 11, and described connecting rod is connected with drive-motor 13 with step-down gear 12 through the bearing at described mounting base middle part.
In the present embodiment, bottom, the inside of described cultivation tank body is provided with cone-shaped body 14, and the aspect ratio of described cultivation tank body is between 0.2-0.3.
Working process:
Motor via reducer drives dial rotation, when the projection on rotating disk is encountered cabochon apron strip, because the bottom of the convexity cabochon apron strip on rotating disk wants high, therefore the projection on rotating disk causes cabochon apron strip jack-up to cultivate tank body integral body by jack-up, cultivate Tank tilting, when being rotated further when driving projection to leave this cabochon apron strip, rotating disk resets under the effect of tank body at spring, when driven by rotary disc projection turns to next cabochon apron strip, cultivating tank body tilts again, and so forth, cultivate tank body and constantly rock the object that reaches stirring, projection on rotating disk of the present utility model will be arranged on the submarginal position of cultivating tank base below, the closer to center, rock mixing effect poorer.
The disclosed technique means of the utility model scheme is not limited only to the disclosed technique means of above-mentioned technique means, also comprises by above technical characterictic and is equal to the technical scheme that replacement forms.
Claims (4)
1. an aerobe cell culture apparatus, comprise mounting base, it is characterized in that: described mounting base is connected and cultivated tank body by one group of spring, the bottom of described cultivation tank body is provided with one group of cabochon apron strip, on described mounting base, be provided with rolling disc, described rolling disc is between the top of described mounting base and the bottom of described cultivation tank body, on described rolling disc, be provided with a hemispherical projections, the height of described hemispherical projections is higher than the bottom of described cabochon apron strip and lower than the bottom of described cultivation tank body.
2. aerobe cell culture apparatus according to claim 1, it is characterized in that: described cultivation tank body top is provided with functional cover, described functional cover comprises the cover plate being connected with described cultivation tank body, is provided with airway, dissolved oxygen rate tester, thermometer through described cover plate on described cover plate.
3. aerobe cell culture apparatus according to claim 1 and 2, it is characterized in that: described mounting base middle part is provided with bearing, described rolling disc middle part is provided with connecting rod, and described connecting rod is connected with drive-motor with step-down gear through the bearing at described mounting base middle part.
4. aerobe cell culture apparatus according to claim 1 and 2, is characterized in that: the bottom, the inside of described cultivation tank body is provided with cone-shaped body, and the aspect ratio of described cultivation tank body is between 0.2-0.3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420295038.1U CN203855589U (en) | 2014-05-29 | 2014-05-29 | Aerobic biological cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420295038.1U CN203855589U (en) | 2014-05-29 | 2014-05-29 | Aerobic biological cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203855589U true CN203855589U (en) | 2014-10-01 |
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ID=51605456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420295038.1U Expired - Fee Related CN203855589U (en) | 2014-05-29 | 2014-05-29 | Aerobic biological cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203855589U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108247316A (en) * | 2018-03-19 | 2018-07-06 | 宁波市伟力剃须用品制造有限公司 | A kind of three layers of cutter head assembling line of full-automatic shaver |
-
2014
- 2014-05-29 CN CN201420295038.1U patent/CN203855589U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108247316A (en) * | 2018-03-19 | 2018-07-06 | 宁波市伟力剃须用品制造有限公司 | A kind of three layers of cutter head assembling line of full-automatic shaver |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141001 Termination date: 20150529 |
|
| EXPY | Termination of patent right or utility model |