CN204509337U - A kind of bacteria culture bottle of adjustable pH value - Google Patents
A kind of bacteria culture bottle of adjustable pH value Download PDFInfo
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- CN204509337U CN204509337U CN201520135761.8U CN201520135761U CN204509337U CN 204509337 U CN204509337 U CN 204509337U CN 201520135761 U CN201520135761 U CN 201520135761U CN 204509337 U CN204509337 U CN 204509337U
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Abstract
The utility model discloses a kind of bacteria culture bottle of adjustable pH value, comprise body, bottleneck, bottle cap, liquid-adding device, wherein body inwall is provided with vertical bar shaped small embossment, and small embossment is provided with pH inductor block; Body is provided with interlayer; Bottleneck is funnel type, and bottle cap central authorities are provided with stirring rod aperture, stirs in rod aperture and is provided with agitator arm; Bottle cap also has liquid-adding device hole, and liquid-adding device is provided with liquid-adding device in hole.The bacteria culture bottle of a kind of adjustable pH value of the present utility model, because self is with pH inductor block and liquid-adding device, the display numerical value of pH inductor block is more accurate, responsive, acid-base modifier is added by liquid-adding device directly in nutrient solution according to the demand difference of potential of hydrogen, can not causing entirety can only changing nutrient solution because of being inconvenient to add acid-base modifier, comparing waste.It is simple that this utility model belongs to a structure, the bacteria culture bottle of convenient and practical adjustable pH value.
Description
Technical field
The utility model relates to a kind of culturing bottle, is specifically a kind of bacteria culture bottle of adjustable pH value.
Background technology
Bacterium, in culturing process, to absorption decomposition and the metabolism of nutrient solution, causes the pH value of nutrient solution to change.Although due to pH value change a lot after nutrition aspect be also applicable to the continued growth of bacterium, the pH value environment of nutrient solution has no longer been applicable to the growth of bacterium, and the cultivation of a small amount of bacterium is all generally change old nutrient solution by new nutrient solution entirety to compare waste.The culturing process of a small amount of bacterium is not easy to sample the changing conditions detecting pH value in addition, general is all add appropriate amount of acid neutralization indicator in nutrient solution, the pH value changing conditions of nutrient solution is observed by the change of indicator color, but some bacterium requires higher to growing environment, can not acid base indicator be added, therefore be badly in need of a kind ofly surveying medium pH value and the bacteria culture bottle of medium pH value being regulated.
Utility model content
In order to overcome above defect, the utility model provides one to survey medium pH value, and the bacteria culture bottle of medium pH value can be regulated, this culturing bottle structure is simple, not only can show the pH value of nutrient solution, can also regulate on a large scale the pH value of nutrient solution and the pH value of nutrient solution be finely tuned.
The purpose of this utility model is realized by following technical scheme:
A bacteria culture bottle for adjustable pH value, comprises body, bottleneck, bottle cap, liquid-adding device, and described body inwall is provided with vertical bar shaped small embossment, and the contact surface of small embossment and body inwall is arc, and described small embossment is provided with pH inductor block; Described body is provided with interlayer, and interlayer outer wall bottom is provided with water inlet, and interlayer outer wall top is provided with water outlet; Described bottleneck is funnel type, and bottleneck is connected with bottle cap sealing; Described bottle cap central authorities establish stirring rod aperture, stir in rod aperture and are provided with agitator arm, and body inside is stretched into through stirring rod aperture in agitator arm one end, and described agitator arm lower end is provided with agitating vane; Tightness system is provided with between described stirring rod aperture and agitator arm; Described bottle cap also has liquid-adding device hole, is provided with long neck funnel type liquid-adding device in described liquid-adding device hole, described liquid-adding device neck end stretches into body inside through liquid-adding device hole, and described liquid-adding device funnel end is reagent storing device I; Described reagent storing device I free end is provided with vertical scale, and reagent storing device I bottom is provided with sealing consent I, and be provided with sealing plug I in sealing consent I, sealing plug I is provided with ventilating pit I.
During use, nutrient solution, bacterial strain are added in culturing bottle, agitator arm is inserted in the stirring rod aperture of bottle cap, bottle cap is built, liquid-adding device neck end is inserted in liquid-adding device hole, by sealing plug I, ventilating pit I is adjusted to transverse direction, required acid-base modifier is added the cultivation that can start bacterium in reagent storing device I.According to the display numerical value of pH inductor block, if the potential of hydrogen of nutrient solution is no longer applicable to the length of bacterium, then stir while by regulating the angle of sealing plug I to regulate the angle of ventilating pit I to tilting or vertically adding in nutrient solution by acid-base modifier, angle by regulating the angle of sealing plug I to regulate ventilating pit I after the pH value of nutrient solution reaches requirement, namely the angular setting of ventilating pit I to level is stopped adding acid-base modifier, nutrient solution stirs, and stops stirring.If nutrient solution needs certain high temperature or low temperature, can stir while carry out temperature control by the liquid passing into certain temperature to water inlet to the nutrient solution in culturing bottle, the liquid of certain temperature flows into from water inlet, and water outlet flows into and the liquid in culturing bottle can be made to keep constant temperature.
In order to realize the purpose of this utility model further, can also by the following technical solutions:
Described liquid-adding device is provided with L-type reagent storing device II in sealing consent I bottom, the described horizontal position of reagent storing device II is provided with sealing consent II, be provided with sealing plug II in described sealing consent II, sealing plug II is provided with ventilating pit II, and described reagent storing device II free end is provided with vertical scale.
Described sealing consent I is flour milling with the contact surface of sealing plug I; Described sealing consent II is flour milling with the contact surface of sealing plug II.
The beneficial effects of the utility model: 1, body is provided with the temperature that interlayer can better keep nutrient solution, the temperature of nutrient solution can also be controlled by the liquid adding certain temperature to water inlet, water inlet under, water outlet can better control upper the flow velocity adding liquid, makes the more uniform temperature of nutrient solution.Vertical bar shaped small embossment is conducive to stirring, and the contact surface of small embossment and culturing bottle inwall is arc, is conducive to the clean of culturing bottle, can not there is dead angle.The soda acid number of degrees of nutrient solution directly can be read by the display numerical value of pH inductor block.Bottleneck is funnel type, is conducive to lid bottle cap, also helps the sealing between bottle cap and bottleneck.The mixing effect of agitating vane is more excellent, and the acid-base modifier added can be made to scatter rapidly, is unlikely to make the acidity or alkalinity of local nutrient solution too high, and acid-base modifier scatter rapidly after nutrient solution potential of hydrogen evenly, the display numerical value of pH inductor block is more accurate.Liquid-adding device can add acid-base modifier when not opening bottle cap in culturing bottle, relatively health, and the ventilating pit I that arranges of the sealing consent I to be arranged by liquid-adding device and sealing plug I can regulate acid-base modifier in right amount add speed, can not that acid-base modifier be caused to add be excessive because of the adding too fast of acid-base modifier.
2, reagent storing device II is the adding apparatus of micro-acid-base modifier, if the pH value claimed range of bacterium to nutrient solution is narrow, drips acid-base modifier by reagent storing device II in nutrient solution.
3, the sealing effectiveness of ground glass surface is better, convenient angle and the dropping acid-base modifier repeatedly adjusting sealing plug.
The bacteria culture bottle of a kind of adjustable pH value of the present utility model, because self is with pH inductor block and liquid-adding device, is not needed to add acid base indicator in inoculum, can be known the potential of hydrogen of nutrient solution by the display numerical value of pH inductor block.The display numerical value of pH inductor block is more accurate, responsive, and can not cause pH sensitive bacterium culture effect is different because different people cultivates because of everyone sight equation.Acid-base modifier is added by liquid-adding device directly in nutrient solution according to the demand difference of potential of hydrogen, and unlike some other small incubation bottle, although because the nutritive ingredient being inconvenient to add acid-base modifier nutrient solution still entirety can also can only change nutrient solution, compare waste.It is simple that this utility model belongs to a structure, the bacteria culture bottle of convenient and practical adjustable pH value.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of the bacteria culture bottle of a kind of adjustable pH value of the utility model.
Fig. 2 is the structure for amplifying schematic diagram of the bacteria culture bottle local location A of a kind of adjustable pH value of the utility model.
Number in the figure: 1 be body, 2 being bottleneck, 3 being bottle cap, 4 be interlayer, 5 be small embossment, 6 being agitator arm for stirring rod aperture, 7,8 be agitating vane, 9 be liquid-adding device, 10 for reagent storing device I, 11 for sealing plug I, 12 for sealing consent I, 13 be water inlet, 14 be water outlet, 15 for sealing plug II, 16 for reagent storing device II, 17 for sealing consent II, 18 for ventilating pit II, 19 for ventilating pit I, 20 is liquid-adding device hole.
Embodiment
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the embodiment of the present invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
As shown in Figure 1-2, for the bacteria culture bottle of a kind of adjustable pH value of the present embodiment, comprise body (1), bottleneck (2), bottle cap (3), liquid-adding device (9), described body (1) inwall is provided with vertical bar shaped small embossment (5), and small embossment (5) is conducive to stirring; Small embossment (5) is arc with the contact surface of body (1) inwall, and arc is conducive to the clean of culturing bottle, can not there is dead angle.Described small embossment (5) is provided with pH inductor block, directly can be read the soda acid number of degrees of nutrient solution by the display numerical value of pH inductor block.Described body is provided with interlayer (4), and interlayer can better keep the temperature of nutrient solution; Interlayer (4) outer wall bottom is provided with water inlet (13), interlayer (4) outer wall top is provided with water outlet (14), the temperature of nutrient solution can also be controlled by the liquid adding certain temperature to water inlet (13), water inlet (13) under, water outlet (14) can better control upper the flow velocity adding liquid, makes the more uniform temperature of nutrient solution.Described bottleneck (2) is funnel type, and bottleneck (2) and bottle cap (3) are tightly connected; Be conducive to lid bottle cap, also help the sealing between bottle cap and bottleneck.Described bottle cap (3) central authorities are provided with stirs rod aperture (6), stir in rod aperture (6) and be provided with agitator arm (7), body inside is stretched into through stirring rod aperture (6) in agitator arm (7) one end, and described agitator arm (7) lower end is provided with agitating vane (8); The mixing effect of agitating vane is more excellent, and the acid-base modifier added can be made to scatter rapidly, is unlikely to make the acidity or alkalinity of local nutrient solution too high, and acid-base modifier scatter rapidly after nutrient solution potential of hydrogen evenly, the display numerical value of pH inductor block is more accurate.Tightness system is provided with between described stirring rod aperture (6) and agitator arm (7); Described bottle cap (3) also has liquid-adding device hole (20), long neck funnel type liquid-adding device (9) is provided with in described liquid-adding device hole (20), described liquid-adding device (9) neck end stretches into body inside through liquid-adding device hole (20), and described liquid-adding device (9) funnel end is reagent storing device I (10); Described reagent storing device I (10) free end is provided with vertical scale, reagent storing device I (10) bottom is provided with sealing consent I (12), be provided with sealing plug I (11) in sealing consent I (12), sealing plug I (11) is provided with ventilating pit I (19).Liquid-adding device (9) can add acid-base modifier when not opening bottle cap (3) in culturing bottle, relatively health, and the ventilating pit I (19) that arranges of the sealing consent I (12) to be arranged by liquid-adding device (3) and sealing plug I (11) can regulate acid-base modifier in right amount add speed, can not that acid-base modifier be caused to add be excessive because of the adding too fast of acid-base modifier.According to the requirement of culturing bacterium to air, if the bacterium cultivated is anerobe, bottle cap (3) can select polytetrafluoroethylmaterial material, sealing effectiveness is better, if the bacterium cultivated is not anerobe, bottle cap (3) then can select ventilation property porous foam silica gel bottle stopper, and this bottle cap not only can purify the air entering culturing bottle, can also ensure the air capacity needed for bacterium.
During use, nutrient solution, bacterial strain are added in culturing bottle, agitator arm (7) is inserted in the stirring rod aperture (6) of bottle cap (3), bottle cap (3) is built, liquid-adding device (9) neck end is inserted in liquid-adding device hole (20), by sealing plug I, ventilating pit I is adjusted to transverse direction.Required acid-base modifier is added the cultivation that can start bacterium in reagent storing device I (10).According to the display numerical value of pH inductor block, if the potential of hydrogen of nutrient solution is no longer applicable to the length of bacterium, stir culture liquid is while by regulating the angle of sealing plug I (11) to regulate the angle of ventilating pit I (19) to tilting or vertically adding in nutrient solution by acid-base modifier on one side, angle by regulating the angle of sealing plug I (11) to regulate ventilating pit (19) I after the pH value of nutrient solution reaches requirement, namely the angular setting of ventilating pit I (19) to level is stopped adding acid-base modifier, can stop after nutrient solution stirs stirring.If nutrient solution needs certain high temperature or low temperature, can stir while the liquid passing through to pass into water inlet (13) certain temperature carries out temperature control to the nutrient solution in culturing bottle, the liquid of certain temperature flows into from water inlet (13), and water outlet (14) flows out and the liquid in culturing bottle can be made to keep constant temperature.The present embodiment arranges electric stirring, is connected by agitator arm with electric blender, and electric blender is fixed by support.
Further, the present embodiment is provided with L-type reagent storing device II (16) in described sealing consent I (12) bottom, the horizontal position of described reagent storing device II (16) is provided with sealing consent II (17), sealing plug II (15) is provided with in described sealing consent II (17), sealing plug II (15) is provided with ventilating pit II (18), and described reagent storing device II (16) free end is provided with vertical scale.The adding apparatus that reagent storing device II (16) is micro-acid-base modifier, if the pH value claimed range of bacterium to nutrient solution is narrow, drips acid-base modifier by reagent storing device II (16) in nutrient solution.
Further, sealing consent I (12) described in the present embodiment with the contact surface of sealing plug I (11) is flour milling; Described sealing consent II (17) is flour milling with the contact surface of sealing plug II (15).The sealing effectiveness of ground glass surface is better, convenient angle and the dropping acid-base modifier repeatedly adjusting sealing plug.
Last it is noted that above embodiment is only in order to illustrate the technical solution of the utility model, be not intended to limit; Although be described in detail the utility model with reference to previous embodiment, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of each embodiment technical scheme of the utility model.
Claims (3)
1. the bacteria culture bottle of an adjustable pH value, comprise body (1), bottleneck (2), bottle cap (3), liquid-adding device (9), it is characterized in that, described body (1) inwall is provided with vertical bar shaped small embossment (5), small embossment (5) is arc with the contact surface of body (1) inwall, and described small embossment (5) is provided with pH inductor block; Described body is provided with interlayer (4), and interlayer (4) outer wall bottom is provided with water inlet (13), and interlayer (4) outer wall top is provided with water outlet (14); Described bottleneck (2) is funnel-form, and bottleneck (2) and bottle cap (3) are tightly connected; Described bottle cap (3) central authorities are provided with stirs rod aperture (6), stir in rod aperture (6) and be provided with agitator arm (7), body inside is stretched into through stirring rod aperture (6) in agitator arm (7) one end, and described agitator arm (7) lower end is provided with agitating vane (8); Described stirring rod aperture (6) and agitator arm (7) contact site are provided with tightness system; Described bottle cap (3) also has liquid-adding device hole (20), long neck funnel type liquid-adding device (9) is provided with in described liquid-adding device hole (20), described liquid-adding device (9) neck end stretches into body inside through liquid-adding device hole (20), and described liquid-adding device (9) funnel end is reagent storing device I (10); Described reagent storing device I (10) free end is provided with vertical scale, reagent storing device I (10) bottom is provided with sealing consent I (12), be provided with sealing plug I (11) in sealing consent I (12), sealing plug I (11) is provided with ventilating pit I (19).
2. the bacteria culture bottle of a kind of adjustable pH value according to claim 1, it is characterized in that described liquid-adding device (9) is provided with L-type reagent storing device II (16) in sealing consent I (12) bottom, the horizontal position of described reagent storing device II (16) is provided with sealing consent II (17), sealing plug II (15) is provided with in described sealing consent II (17), sealing plug II (15) is provided with ventilating pit II (18), and described reagent storing device II (16) free end is provided with vertical scale.
3. the bacteria culture bottle of a kind of adjustable pH value according to claim 1 and 2, is characterized in that described sealing consent I (12) is flour milling with the contact surface of sealing plug I (11); Described sealing consent II (17) is flour milling with the contact surface of sealing plug II (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520135761.8U CN204509337U (en) | 2015-03-10 | 2015-03-10 | A kind of bacteria culture bottle of adjustable pH value |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520135761.8U CN204509337U (en) | 2015-03-10 | 2015-03-10 | A kind of bacteria culture bottle of adjustable pH value |
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| Publication Number | Publication Date |
|---|---|
| CN204509337U true CN204509337U (en) | 2015-07-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520135761.8U Expired - Fee Related CN204509337U (en) | 2015-03-10 | 2015-03-10 | A kind of bacteria culture bottle of adjustable pH value |
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| CN (1) | CN204509337U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490198A (en) * | 2018-03-26 | 2018-09-04 | 郭庆 | A kind of discrimination method of bacterium |
| CN112538427A (en) * | 2020-11-16 | 2021-03-23 | 中国人民解放军陆军军医大学第二附属医院 | Intestinal lymphocyte culture apparatus |
-
2015
- 2015-03-10 CN CN201520135761.8U patent/CN204509337U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490198A (en) * | 2018-03-26 | 2018-09-04 | 郭庆 | A kind of discrimination method of bacterium |
| CN108490198B (en) * | 2018-03-26 | 2021-10-19 | 英特莱检验检测认证(山东)有限公司 | Bacteria identification method |
| CN112538427A (en) * | 2020-11-16 | 2021-03-23 | 中国人民解放军陆军军医大学第二附属医院 | Intestinal lymphocyte culture apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150729 Termination date: 20170310 |