CN108490198A - A kind of discrimination method of bacterium - Google Patents
A kind of discrimination method of bacterium Download PDFInfo
- Publication number
- CN108490198A CN108490198A CN201810252824.6A CN201810252824A CN108490198A CN 108490198 A CN108490198 A CN 108490198A CN 201810252824 A CN201810252824 A CN 201810252824A CN 108490198 A CN108490198 A CN 108490198A
- Authority
- CN
- China
- Prior art keywords
- reagent
- bottle
- cavity
- identification
- sliding slot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/0092—Scheduling
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to Bacteria Identification technical fields, specifically a kind of discrimination method of bacterium, the identification method uses following identification systems, the identification systems include fixing body, power plant module, reagent adding device, agitating unit and identification box, and cavity one, cavity two and cavity three are provided on fixing body；Identification box is located at cavity three, identifies in box and fills bacterium to be identified；Power plant module is located at fixing body upper end, and power plant module is used to provide power for agitating unit；Reagent adding device is located at cavity one, and reagent adding device to identification box for adding plurality of reagents successively, and reagent adding device is at least provided with 2；Agitating unit is located at cavity two, and agitating unit is connect with power plant module, and agitating unit is for stirring bacterium to be identified and reagent in identification box, and agitating unit is at least provided with 2；The present invention improves reaction speed when bacterium mixes with reagent and improves the efficiency of Bacteria Identification and the accuracy of cellular identification.
Description
Technical field
The invention belongs to Bacteria Identification technical field, specifically a kind of discrimination method of bacterium.
Background technology
The identification of bacterium is that the inspection of various aspects is carried out to bacterium, the identification of bacterium by being separately cultured the bacterium of acquisition,
The pure culture degree without containing other microorganisms must be reached, system identification could be carried out.System identification is exactly to pass through pathogen
The detections such as morphosis, growth characteristics, antigenicity and pathogenicity, be used in combination known standard immunoassay serum to determine separation of bacterial
Genus and species and type.The program of microbial identification is typically according to its form, fixed kind of growth, biochemical characteristic etc., finally according to antigen
Immunoserology inspection is shaped.The form of the various bacteriums of morphological examination is metastable under suitable environment.But environment
Change, such as the change of culture medium condition, the effect etc. of antibiotic and chemicals can make bacterium generate irregular shape
State, and may occur in which the defect and diversity of cell wall.
It, can be with the identification method and operating procedure of general a set of specification, by a set of rational in many Bacteria Identifications
Identification method can save a large amount of qualification times, improve determination rates, avoid making unnecessary operation in qualification process and unrestrained
It is time-consuming, there is the identical same or analogous identification method of identification method to sort out some different bacteriums, is conducive to this
The Rapid identification of a little bacteriums, meanwhile, using special a set of identification systems, it is also beneficial to improve determination rates and the identification of bacterium
Accuracy.
In consideration of it, a kind of discrimination method of bacterium of the present invention, provide a kind of for needing to be identified with reagent
The identification method of bacterium can improve the speed of Bacteria Identification process with specification Bacteria Identification flow.
Invention content
In order to make up for the deficiencies of the prior art, the present invention proposes a kind of discrimination method of bacterium, present invention is mainly used for
Identification identification to bacterium, the use of specification Bacteria Identification flow and instrument improve the efficiency of Bacteria Identification process.The present invention is logical
Cooperating with each other for power plant module and agitating unit is crossed, reaction speed when bacterium mixes with reagent is improved, is improved thin
The efficiency of the dientification of bacteria, meanwhile, the present invention is by reagent adding device and identifies cooperating with each other for box, is effectively simplified and is seeing
The step of reagent is manually added when examining experimental phenomena, it is ensured that the concentration of user's attention improves the accurate of cellular identification
Property.
The technical solution adopted by the present invention to solve the technical problems is：A kind of discrimination method of bacterium, the identification method
Using following identification systems, which includes fixing body, power plant module, reagent adding device, agitating unit and identifies box,
Cavity one, cavity two and cavity three are provided on the fixing body；The identification box is located at cavity three, identifies to fill in box and wait for
Identify bacterium；The power plant module is located at fixing body upper end, and power plant module is used to provide power for agitating unit；The reagent adds
Unit is added to be located at cavity one, reagent adding device to identification box for adding plurality of reagents successively, and reagent adding device is extremely
It is provided with 2 less；The agitating unit is located at cavity two, and agitating unit is connect with power plant module, and agitating unit is for stirring
Identify bacterium to be identified and reagent in box, and agitating unit is at least provided with 2；
The discrimination of bacteria method includes the following steps：
Step 1：Reagent one is configured, after sterilization treatment and is fitted into reagent bottle one, configures reagent two, after sterilization treatment simultaneously
It is fitted into reagent bottle two；
Step 2：By in step 1 reagent bottle one and reagent bottle two be respectively put into different bottle clips and be clamped, make reagent
The syringe needle plug of bottle downward and is directed at reagent runner, and blend rubber set entangles rubber vent plug one end of reagent bottle；
Step 3：After the completion of waiting for step 2, the discriminating box equipped with bacterium solution to be tested is put at the cavity three of identification system,
And discriminating box is made to be located at immediately below hollow guide rod；
Step 4：Start motor one, makes motor one that hollow guide rod be driven to be slided up and down in sliding slot two, hollow guide rod passes through
Shaft drives first axle rotation, first axle to drive agitator arm rotation, and the agitator arm in first axle is to be tested in step 2
Bacterium solution is sufficiently stirred, and bacterium solution to be tested is avoided to precipitate；
Step 5：Start automatic ventilation valve door, the gas flow automatic ventilation valve door in gas cylinder, automatic ventilation valve door timing
Start, automatically control duration of ventilation, it is quantitative gas to make the gas by automatic ventilation valve door；Gas enters reagent bottle one, fixed
The reagent one in gas compression quantitative reagent bottle one is measured, reagent one is entered after quantitative extrusion in reagent flow road, and reagent one passes through
Reagent runner enters in hollow guide rod, and the quantitative reagent in hollow guide rod, which will enter, to be differentiated in box, by stirring module stirring, is made
Reagent one is sufficiently mixed with by the bacterium solution to be tested in step 2, after waiting for fully reaction, is obtained mixed solution one, is seen by phenomenon
It examines, obtain a kind of identification result and records；
Step 6：Start automatic ventilation valve door, the gas flow automatic ventilation valve door in gas cylinder, automatic ventilation valve door timing
Start, automatically control duration of ventilation, it is quantitative gas to make the gas by automatic ventilation valve door；Gas enters reagent bottle two, fixed
The reagent two in gas compression quantitative reagent bottle two is measured, reagent two is entered after quantitative extrusion in reagent flow road, and reagent two passes through
Reagent runner enters in hollow guide rod, and quantitative reagent two enters in turn to be differentiated in box, by stirring module stirring, makes reagent two
It is sufficiently mixed with the mixed solution one in step 5, after waiting for fully reaction, obtains mixed solution two, by Phenomena Observation, obtain
Another identification result simultaneously records；
Step 7：Cleaning experiment instrument simultaneously does sterilization treatment.
The power plant module includes motor one, turntable, connecting rod, sliding block one and hollow guide rod, the fixing body upper end setting
There is sliding slot one, sliding block one is located in sliding slot one and sliding block one is slidably connected with sliding slot one；The turntable is located at fixing body upper end；Institute
It states motor one and is located at turntable side, the rotation of one driving disc of motor；One end of the connecting rod is hinged with disk edge, connecting rod it is another
One end is hinged with sliding block one；Sliding slot two is provided on the fixing body, sliding slot two and sliding slot one are located on the same line；It is described
Hollow guide rod is located in second sliding slot, and hollow guide rod is fixedly connected with sliding block one, and the lower end of hollow guide rod is hollow shape；Motor one
Hollow guide rod is driven to be slided up and down in sliding slot two by turntable, connecting rod and sliding block one.In use, starting motor one, motor one
Rotation drives turntable rotation, turntable to drive sliding block one to slidably reciprocate in sliding slot one by connecting rod, and sliding block one drives hollow guide rod
It is slided up and down in sliding slot two.
The reagent adding device includes gas cylinder, reagent bottle, tracheae and automatic ventilation valve door, is provided on the fixing body
Multiple bottle clips, and bottle clip is located at cavity one, bottle clip is for being clamped reagent bottle；The reagent bottle include bottle, rubber vent plug and
Syringe needle plug；The rubber vent plug is installed on bottle and sealed bottle body, and rubber vent plug center is provided with multiple pores, rubber vent plug
When no ambient pressure acts on, rubber vent plug is airtight；Syringe needle plug is installed on bottle lower end and sealed bottle body lower end, needle chock plug
On syringe needle be capillary syringe needle, under no ambient pressure, reagent will not flow out from needle chock plug；The gas cylinder is located on cavity one
Portion, tracheae are located at reagent bottle upper end, and automatic ventilation valve door is between gas cylinder and tracheae, and automatic ventilation valve door one end and gas cylinder
The upper end unicom of unicom, the automatic ventilation valve door other end and tracheae；Rubber sleeve, and rubber sleeve set is arranged in the lower end of the tracheae
It is located at reagent bottle upper end；Be provided with multiple reagent runners on the fixing body, one end of reagent runner be located at syringe needle plug just under
The through-hole unicom being arranged on side, the other end of reagent runner and hollow guide rod.In use, starting automatic ventilation valve door, in gas cylinder
Gas flow automatic ventilation valve door, automatic ventilation valve door start by set date automatically controls duration of ventilation, makes to pass through automatic ventilation valve
The gas of door is quantitative gas, and gas enters reagent bottle by tracheae, and quantitative gas compresses the reagent in quantitative reagent bottle, reagent
From from syringe needle plug in runner reagent runner, reagent is entered by reagent runner in hollow guide rod, and quantitative reagent is from hollow guide rod
It is middle to enter in identification box.
The automatic ventilation valve door includes controller, magnetic ball, electromagnet, spring one, and the controller is located at fixing body
On outer wall；It is provided with gas port at the cavity one；The electromagnet is located at gas port top, and ventilation is provided on electromagnet
Hole is connected between electromagnet and controller by conducting wire, and electromagnet makes gas port be in aeration status for magnetic ball to be sucked；Institute
It states magnetic ball and is located at gas port lower end, magnetic ball is for blocking gas port；The spring one is between electromagnet and magnetic ball
And spring one is used to magnetic ball being pressed on gas port lower end；The upper end of the gas port and gas cylinder unicom, the lower end of gas port
With tracheae unicom, and flowmeter is provided on gas-guide tube, flowmeter is connect with controller, and flowmeter flows through gas-guide tube for detecting
Gas flow.In use, magnetic ball is against at the aperture of gas port by spring one, automatic ventilation valve door is in closed state,
Start automatic ventilation valve behind the door, controller timing controlled electromagnet adsorbs magnetic ball, the decrement that spring one is compressed by magnetic ball
It increases, magnetic ball is left at the aperture of gas port, and automatic ventilation valve door is in aeration status, and the gas in gas cylinder passes through electromagnet
On venthole circulate downwards, gas pass through flowmeter when, flowmeter meter goes out the gas volume flowed through and feeds back to controller, control
Device analysis and Control electromagnet timing energization processed adsorbs magnetic ball, and the gas flow for flowing through automatic ventilation valve door is controlled with this,
Gas after gas port by entering in tracheae.
The agitating unit include shaft, torsional spring, elastic string one, elastic string two, resetting spring, the first axle sleeve, first axle,
Second axis, thrust ball bearing and sliding block two, the shaft are located in cavity one, and shaft is rotatablely connected with hollow guide rod；It is described
One end of torsional spring is connect with shaft, and the other end of torsional spring is fixed on one inner wall of cavity；Sliding slot two is provided on the fixing body,
Sliding slot two is in 20 ° to 45 ° angles with vertical direction；First axle sleeve is located on the same line with sliding slot one；The sliding block
Two are located in sliding slot two, and sliding block two is slidably connected with sliding slot two, and one end of sliding block two is fixedly connected with the second axis upper end, sliding block
The other end connect with resetting spring, resetting spring is located in sliding slot two；One end of the elastic string one is solid with one inner wall of cavity
Fixed connection, the other end of elastic string one are wrapped in shaft；One end of the elastic string two is fixed in shaft, elastic string two
The other end passes through resetting spring and is fixed on sliding block two；Thrust ball bearing is provided between the first axle and the second axis, the
One axis lower end is connect with thrust ball bearing upper end, and the upper end of the second axis and the lower end of thrust ball bearing connect, and thrust ball bearing is used
To realize the relative rotation of first axle and the second axis；The first spiral chute, first axle end are provided on the outer curved wall of the first axle
End is provided with agitator arm, and agitator arm is used for stirring；First sleeve lining is equipped with the first bulb, and the first bulb
It is adapted with the first spiral chute, first axle is connect with the first axle sleeve rotational slide, and first axle moves up and down in the first axle sleeve
It will realize first axle positive and negative rotation while moving up and down.In use, hollow guide rod slides up and down in sliding slot two, it is hollow to lead
Bar drives shaft to move up and down, and when shaft moves up, the elastic string one being wrapped in shaft is pulled down, and shaft rotates forward, in torsional spring
Elastic potential energy is rolled up and deposits, elastic string two pulls up sliding block two, and sliding block two drives the second axis to move up, and the second axis passes through thrust ball bearing
First axle is driven to be moved up at the first axle sleeve, at this point, resetting spring is compressed, when first axle moves up in the first axle sleeve, first
Axis rotates forward, and first axle rotation drives agitator arm to rotate forward；When shaft moves down, is dissipated outside torsional spring and discharge elastic potential
Can, shaft inverts and winds elastic string one, and elastic string two will relax, and resetting spring resets, and sliding block two moves down and drives the second axis
It moves down, the second axis drives first axle to be moved down at the first axle sleeve by thrust ball bearing, and first axle rotates backward, first axle rotation
Agitator arm is driven to rotate backward；First axle moves up and down in the first axle sleeve realizes first axle while moving up and down just
Reverse turn, to realize the agitating function of stirring module.
The beneficial effects of the invention are as follows：
1. a kind of discrimination method of bacterium of the present invention, tailor of the present invention it is a set of by identification systems and with plus
Enter reagent and generate the method for reacting phenomenon to identify bacterial species, by standard operation flow, reduces unnecessary operating procedure,
Improve the efficiency of Bacteria Identification and the accuracy of Bacteria Identification.
2. a kind of discrimination method of bacterium of the present invention, the phase that the present invention passes through power plant module and agitating unit
Mutual cooperating, mixing of the power plant module driving stirring module to bacterium solution and reagent, allows bacterium solution and reagent quickly to fill
Divide mixing, improve the reaction speed of bacterium solution and reagent, and then improve the identification speed of bacterium, and then the identification for improving bacterium is imitated
Rate.
3. a kind of discrimination method of bacterium of the present invention, the present invention is by reagent adding device and identifies the mutual of box
Cooperating, reagent adding device can timing according to quantity add reagent to identification box, eliminate the trouble of artificial addition reagent,
It ensures that attention of the user in observation experiment phenomenon is concentrated, improves the accuracy of cellular identification.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings.
Fig. 1 is flow chart of the method for the present invention；
Fig. 2 is the overall structure diagram of the present invention；
Fig. 3 is the connection diagram of the automatic ventilation valve door and reagent bottle of the present invention；
Fig. 4 is the first axle and the second axis connection schematic diagram of the present invention；
Fig. 5 is the agitator arm structural schematic diagram of the present invention；
In figure：Fixing body 1, cavity 2 12, cavity 3 13, bottle clip 14, power plant module 2, motor 1, turns cavity 1
Disk 22, sliding block 1, hollow guide rod 24, agitating unit 3, gas cylinder 31, reagent bottle 32, bottle 321, tracheae 33, automatic ventilation valve
Door 34, controller 341, magnetic ball 342, electromagnet 343, reagent adding device 4, shaft 41, torsional spring 42, elastic string 1, bullet
Property rope 2 44, resetting spring 45, the first axle sleeve 46, first axle 47, the second axis 48, thrust ball bearing 49 and identification box 5.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific implementation mode is closed, the present invention is further explained.
As shown in Figures 1 to 5, a kind of discrimination method of bacterium, the identification method use following identification systems, the identification system
System includes fixing body 1, power plant module 2, agitating unit 3, reagent adding device 4 and identification box 5, is provided on the fixing body 1
Cavity 1, cavity 2 12 and cavity 3 13；The identification box 5 is located at cavity 3 13, is filled in identification box 5 to be identified thin
Bacterium；The power plant module 2 is located at 1 upper end of fixing body, and power plant module 2 is used to provide power for reagent adding device 4；The stirring
Unit 3 is located at cavity 1, and agitating unit 3 to identification box 5 for adding plurality of reagents successively, and agitating unit 3 is at least set
It is equipped with 2；The reagent adding device 4 is located at cavity 2 12, and reagent adding device 4 is connect with power plant module 2, reagent addition
Unit 4 is for stirring bacterium to be identified and reagent in identification box 5, and reagent adding device 4 is at least provided with 2；
The discrimination of bacteria method includes the following steps：
Step 1：Reagent one is configured, after sterilization treatment and is fitted into reagent bottle one, configures reagent two, after sterilization treatment simultaneously
It is fitted into reagent bottle two；
Step 2：By in step 1 reagent bottle one and reagent bottle two be respectively put into different bottle clips 14 and be clamped, make examination
The syringe needle plug of agent bottle 32 downward and is directed at reagent runner, and blend rubber set entangles rubber vent plug one end of reagent bottle 32；
Step 3：After the completion of waiting for step 2, the discriminating box 5 equipped with bacterium solution to be tested is put into the cavity 3 13 of identification system
Place, and discriminating box 5 is made to be located at immediately below hollow guide rod 24；
Step 4：Start motor 1, makes motor 1 that hollow guide rod 24 be driven to be slided up and down in sliding slot two, it is hollow to lead
Bar 24 drives first axle 47 to rotate by shaft 41, and first axle 47 drives agitator arm 471 to rotate, the paddle in first axle 47
Bacterium solution to be tested is sufficiently stirred in 471 pairs of step 2 of wheel, and bacterium solution to be tested is avoided to precipitate；
Step 5：Start automatic ventilation valve door 34, the gas flow automatic ventilation valve door 34 in gas cylinder 31, automatic ventilation valve
34 start by set date of door, automatically control duration of ventilation, and it is quantitative gas to make the gas by automatic ventilation valve door 34；Gas enters examination
Agent bottle one, quantitative gas compress the reagent one in quantitative reagent bottle one, and reagent one is entered after quantitative extrusion in reagent flow road, examination
Agent one is entered by reagent runner in hollow guide rod 24, and the quantitative reagent in hollow guide rod 24, which will enter, to be differentiated in box 5, by stirring
Module stirring is mixed, reagent one is made to be sufficiently mixed with by the bacterium solution to be tested in step 2, after waiting for fully reaction, obtains mixed solution
One, by Phenomena Observation, obtains a kind of identification result and record；
Step 6：Start automatic ventilation valve door 34, the gas flow automatic ventilation valve door 34 in gas cylinder 31, automatic ventilation valve
34 start by set date of door, automatically control duration of ventilation, and it is quantitative gas to make the gas by automatic ventilation valve door 34；Gas enters examination
Agent bottle two, quantitative gas compress the reagent two in quantitative reagent bottle two, and reagent two is entered after quantitative extrusion in reagent flow road, examination
Agent two is entered by reagent flow road in hollow guide rod 24, and quantitative reagent two enters in turn to be differentiated in box 5, is stirred by stirring module
It mixes, reagent two is made to be sufficiently mixed with the mixed solution one in step 5, after waiting for fully reaction, mixed solution two is obtained, by existing
As observation, obtains another identification result and record；
Step 7：Cleaning experiment instrument simultaneously does sterilization treatment.
The power plant module 2 includes motor 1, turntable 22, connecting rod, sliding block 1 and hollow guide rod 24, the fixing body
1 upper end is provided with sliding slot one, and sliding block 1 is located in sliding slot one and sliding block 1 is slidably connected with sliding slot one；The turntable 22
In 1 upper end of fixing body；The motor 1 is located at 22 side of turntable, the rotation of one 21 driving disc 22 of motor；The one of the connecting rod
End is hinged with 22 edge of turntable, and the other end and sliding block 1 of connecting rod are hinged；Sliding slot two, sliding slot are provided on the fixing body 1
Two and sliding slot one be located on the same line；The hollow guide rod 24 is located in second sliding slot, hollow guide rod 24 and sliding block 1
It is fixedly connected, the lower end of hollow guide rod 24 is hollow shape；Motor 1 drives hollow lead by turntable 22, connecting rod and sliding block 1
Bar 24 slides up and down in sliding slot two.In use, starting motor 1, the rotation of motor 1 drives turntable 22 to rotate, turntable 22
Sliding block 1 is driven to slidably reciprocate in sliding slot one by connecting rod, sliding block 1 drives hollow guide rod 24 is upper in sliding slot two to glide
It is dynamic.
The agitating unit 3 includes gas cylinder 31, reagent bottle 32, tracheae 33 and automatic ventilation valve door 34, on the fixing body 1
Multiple bottle clips 14 are provided with, and bottle clip 14 is located at cavity 1, bottle clip 14 is for being clamped reagent bottle 32；The reagent bottle 32 wraps
Include bottle 321, rubber vent plug and syringe needle plug；The rubber vent plug is installed on bottle 321 and sealed bottle body 321, in rubber vent plug
Centre is provided with multiple pores, and for rubber vent plug when no ambient pressure acts on, rubber vent plug is airtight；Syringe needle plug is installed on bottle
321 lower end of body and 321 lower end of sealed bottle body, the syringe needle on needle chock plug is capillary syringe needle, and under no ambient pressure, reagent will not be from
It is flowed out at needle chock plug；The gas cylinder 31 is located at one 11 top of cavity, and tracheae 33 is located at 32 upper end of reagent bottle, automatic ventilation valve door 34
Between gas cylinder 31 and tracheae 33, and 34 one end of automatic ventilation valve door and 31 unicom of gas cylinder, 34 other end of automatic ventilation valve door
With the upper end unicom of tracheae 33；Rubber sleeve is arranged in the lower end of the tracheae 33, and rubber sleeve is set in 32 upper end of reagent bottle；Institute
State and be provided with multiple reagent runners on fixing body 1, one end of reagent runner is located at the underface of syringe needle plug, reagent runner it is another
The through-hole unicom being arranged on end and hollow guide rod 24.In use, starting automatic ventilation valve door 34, the gas flow in gas cylinder 31 is certainly
Dynamic gas valve 34,34 start by set date of automatic ventilation valve door automatically control duration of ventilation, make the gas by automatic ventilation valve door 34
Body is quantitative gas, and gas enters reagent bottle 32 by tracheae 33, and quantitative gas compresses the reagent in quantitative reagent bottle 32, reagent
From in runner reagent runner, reagent is entered by reagent runner in hollow guide rod 24, and quantitative reagent is led from hollow from syringe needle plug
Enter in bar 24 in identification box 5.
The automatic ventilation valve door 34 includes controller 341, magnetic ball 342, electromagnet 343 and spring one, the control
Device 341 is located on 1 outer wall of fixing body；It is provided with gas port at the cavity 1；The electromagnet 343 and spring one, which are located at, leads
It is provided with venthole on stomata top, electromagnet 343 and spring one, passes through between electromagnet 343 and spring one and controller 341
Conducting wire connects, and electromagnet 343 and spring one make gas port be in aeration status for magnetic ball 342 to be sucked；The magnetic ball 342
Positioned at gas port lower end, magnetic ball 342 is for blocking gas port；The spring one is located at electromagnet 343 and spring one and magnetism
Between ball 342 and spring one is used to magnetic ball 342 being pressed on gas port lower end；The upper end of the gas port and gas cylinder 31
It is logical, lower end and 33 unicom of tracheae of gas port, and flowmeter is provided on gas-guide tube 33, flowmeter is connect with controller 341, stream
Gauge is for detecting the gas flow for flowing through gas-guide tube 33.In use, magnetic ball 342 is against the aperture of gas port by spring one
Place, automatic ventilation valve door 34 are in closed state, after starting automatic ventilation valve door 34,341 timing controlled electromagnet 343 of controller
Magnetic ball 342 is adsorbed with spring one, the decrement that spring one is compressed by magnetic ball 342 increases, and magnetic ball 342 leaves gas port
At aperture, automatic ventilation valve door 34 is in aeration status, and the gas in gas cylinder 31 passes through the ventilation on electromagnet 343 and spring one
Hole is circulated downwards, and when gas passes through flowmeter, flowmeter meter goes out the gas volume flowed through and feeds back to controller 341, controller
341 analysis and Control electromagnet 343 and one timing energization of spring adsorb magnetic ball 342, and are controlled with this and flow through automatic ventilation valve
The gas flow of door 34, gas after gas port by entering in tracheae 33.
The reagent adding device 4 include shaft 41, torsional spring 42, elastic string 1, elastic string 2 44, resetting spring 45,
First axle sleeve 46, first axle 47, the second axis 48, thrust ball bearing 49 and sliding block two, the shaft 41 are located in cavity 1, and
Shaft 41 is rotatablely connected with hollow guide rod 24；One end of the torsional spring 42 is connect with shaft 41, and the other end of torsional spring 42 is fixed on
On one 11 inner wall of cavity；Sliding slot two is provided on the fixing body 1, sliding slot two is in 20 ° to 45 ° angles with vertical direction；It is described
First axle sleeve 46 is located on the same line with sliding slot one；The sliding block two is located in sliding slot two, and sliding block two is slided with sliding slot two
Dynamic connection, one end of sliding block two are fixedly connected with 48 upper end of the second axis, and the other end of sliding block is connect with resetting spring 45, reset bullet
Spring 45 is located in sliding slot two；One end of the elastic string 1 is fixedly connected with one 11 inner wall of cavity, elastic string 1 it is another
End is wrapped in shaft 41；One end of the elastic string 2 44 is fixed in shaft 41, and the other end of elastic string 2 44 passes through again
Position spring 45 is simultaneously fixed on sliding block two；It is provided with thrust ball bearing 49, first axle between the first axle 47 and the second axis 48
47 lower ends are connect with 49 upper end of thrust ball bearing, and the upper end of the second axis 48 is connect with the lower end of thrust ball bearing 49, thrust ball axis
49 are held to realize the relative rotation of first axle 47 and the second axis 48；It is provided with the first spiral slide on 47 outer curved wall of the first axle
Slot, 47 end of first axle are provided with agitator arm, and agitator arm is used for stirring；First axle sleeve, 46 inner wall is equipped with first
Bulb, and the first bulb is adapted with the first spiral chute, first axle 47 is connect with 46 rotational slide of the first axle sleeve, first axle 47
Positive and negative rotation while moving up and down of first axle 47 will be realized by moving up and down in the first axle sleeve 46.In use, hollow guide rod
24 slide up and down in sliding slot two, and hollow guide rod 24 drives shaft 41 to move up and down, and when shaft 41 moves up, is wrapped in shaft 41
On elastic string 1 be pulled down, shaft 41 rotates forward, and torsional spring 42 is involute simultaneously to deposit elastic potential energy, and elastic string 2 44 will be on sliding block two
It draws, sliding block two drives the second axis 48 to move up, and the second axis 48 drives first axle 47 at the first axle sleeve 46 by thrust ball bearing 49
It moves up, at this point, resetting spring 45 is compressed, when first axle 47 moves up in the first axle sleeve 46, first axle 47 rotates forward, first
The rotation of axis 47 drives agitator arm 371 to rotate forward；When shaft 41 moves down, is dissipated outside torsional spring 42 and discharge elastic potential energy, shaft
41 invert and wind elastic string 1, and elastic string 2 44 will relax, and resetting spring 45 resets, and sliding block two moves down and drives the second axis
48 move down, and the second axis 48 drives first axle 47 to be moved down at the first axle sleeve 46 by thrust ball bearing 49, and first axle 47 reversely turns
Dynamic, the rotation of first axle 47 drives agitator arm 371 to rotate backward；First axle 47 moves up and down in the first axle sleeve 46 realizes
One positive and negative rotation while moving up and down of axis 47, to realize the agitating function of stirring module.
Concrete operations flow is as follows：
First, reagent bottle 32 is put into bottle clip 14 and is clamped, make the syringe needle plug of reagent bottle 32 downward and be directed at reagent runner,
Blend rubber set entangles rubber vent plug one end of reagent bottle 32；Identification box 5 equipped with bacterium solution to be tested is put into identification systems
At cavity 3 13, and identification box 5 is made to be located at immediately below hollow guide rod 24；
Start motor 1, the rotation of motor 1 drives turntable 22 to rotate, and turntable 22 drives sliding block 1 to exist by connecting rod
It slidably reciprocates in sliding slot one, sliding block 1 drives hollow guide rod 24 to be slided up and down in sliding slot two, and hollow guide rod 24 drives shaft
41 move up and down, and when shaft 41 moves up, the elastic string 1 being wrapped in shaft 41 is pulled down, and shaft 41 rotates forward, torsional spring 42
Curls inward simultaneously deposits elastic potential energy, and elastic string 2 44 pulls up sliding block two, and sliding block two drives the second axis 48 to move up, and the second axis 48 is by pushing away
Power ball bearing 49 drives first axle 47 to be moved up at the first axle sleeve 46, at this point, resetting spring 45 is compressed, first axle 47 is first
When being moved up in axle sleeve 46, first axle 47 rotates forward, and the rotation of first axle 47 drives agitator arm 371 to rotate forward；In shaft 41
When moving down, elastic potential energy is dissipated and discharged outside torsional spring 42, shaft 41 inverts and winds elastic string 1, and elastic string 2 44 will relax,
Resetting spring 45 resets, and sliding block two moves down and the second axis 48 is driven to move down, and the second axis 48 drives first by thrust ball bearing 49
Axis 47 moves down at the first axle sleeve 46, and first axle 47 rotates backward, and the rotation of first axle 47 drives agitator arm 371 to rotate backward；
First axle 47 moves up and down in the first axle sleeve 46 realizes positive and negative rotation while moving up and down of first axle 47, to realize
Stir the agitating function of module；
Start automatic ventilation valve door 34, the gas flow automatic ventilation valve door 34 in gas cylinder 31, spring one is by magnetic ball 342
It being against at the aperture of gas port, automatic ventilation valve door 34 is in closed state, after starting automatic ventilation valve door 34, controller 341
Timing controlled electromagnet 343 and spring one adsorb magnetic ball 342, and the decrement that spring one is compressed by magnetic ball 342 increases, magnetic
Ball 342 leaves at the aperture of gas port, and automatic ventilation valve door 34 is in aeration status, and the gas in gas cylinder 31 passes through electromagnet
343 and spring one on venthole circulate downwards, gas pass through flowmeter when, flowmeter meter goes out the gas volume flowed through and feedback
To controller 341,341 analysis and Control electromagnet 343 of controller and one timing energization of spring adsorb magnetic ball 342, and with this
The gas flow of automatic ventilation valve door 34 is flowed through in control, and for gas by entering in tracheae 33 after gas port, gas passes through tracheae 33
Into reagent bottle 32, quantitative gas compresses the reagent in quantitative reagent bottle 32, and reagent is flowed to from syringe needle plug in reagent runner, tries
Agent by reagent runner enter hollow guide rod 24 in, quantitative reagent from hollow guide rod 24 enter identification box 5 in.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle
It is fixed.
Claims (5)
1. a kind of discrimination method of bacterium, it is characterised in that：The identification method uses following identification systems, which includes
Fixing body (1), power plant module (2), agitating unit (3), reagent adding device (4) and identification box (5), on the fixing body (1)
It is provided with cavity one (11), cavity two (12) and cavity three (13)；The identification box (5) is located at cavity three (13), identifies box
(5) bacterium to be identified is filled in；The power plant module (2) is located at fixing body (1) upper end, and power plant module (2) for reagent for adding
Unit (4) is added to provide power；The agitating unit (3) is located at cavity one (11), and agitating unit (3) is for successively to identifying box
(5) plurality of reagents is added, and agitating unit (3) is at least provided with 2；The reagent adding device (4) is located at cavity two (12)
Place, reagent adding device (4) are connect with power plant module (2), and reagent adding device (4) is to be identified in identification box (5) for stirring
Bacterium and reagent, and reagent adding device (4) is at least provided with 2；
The method for determining bacteria includes the following steps：
Step 1：Reagent one is configured, after sterilization treatment and is fitted into reagent bottle one, configures reagent two, after sterilization treatment and is packed into
In reagent bottle two；
Step 2：The reagent bottle one obtained in step 1 and reagent bottle two are respectively put into clamping in different bottle clip (14), made
The syringe needle plug of reagent bottle (32) downward and is directed at reagent runner, and blend rubber set entangles rubber vent plug one end of reagent bottle (32)；
Step 3：After the completion of waiting for step 2, the identification box (5) equipped with bacterium solution to be tested is put into the cavity three (13) of identification systems
Place；
Step 4：Start power plant module (2), makes power plant module (2) that stirring module be driven to carry out bacterium solution to be tested in step 2
It is sufficiently stirred, bacterium solution to be tested is avoided to precipitate；
Step 5：Start agitating unit (3), makes agitating unit (3) automatically by the reagent one of the reagent bottle one in step 2 to mirror
Determine that aequum agent is added in box (5), by stirring module stirring, so that reagent one is sufficiently mixed with bacterium solution to be tested, wait for abundant
After reaction, mixed solution one is obtained, by Phenomena Observation, a kind of qualification result is obtained and records；
Step 6：After the completion of waiting for step 5, make agitating unit (3) automatically by the reagent two of the reagent bottle two in step 2 to identification
Aequum agent is added in box (5), stirring module is stirred, and reagent two is made to be sufficiently mixed with mixed solution one, is waited for fully anti-
Ying Hou obtains mixed solution two, by Phenomena Observation, obtains another qualification result and records；
Step 7：After step 6 completion, cleaning experiment instrument simultaneously does sterilization treatment.
2. a kind of discrimination method of bacterium according to claim 1, it is characterised in that：The power plant module (2) includes electricity
Machine one (21), turntable (22), connecting rod, sliding block one (23) and hollow guide rod (24), fixing body (1) upper end are provided with sliding slot
One, sliding block one (23) is located in sliding slot one and sliding block one (23) is slidably connected with sliding slot one；The turntable (22) is located at fixing body
(1) upper end；The motor one (21) is located at turntable (22) side, motor one (21) driving disc (22) rotation；The connecting rod
One end and turntable (22) edge are hinged, and the other end and sliding block one (23) of connecting rod are hinged；It is provided with sliding slot on the fixing body (1)
Two, sliding slot two and sliding slot one are located on the same line；The hollow guide rod (24) is located in second sliding slot, hollow guide rod (24)
It is fixedly connected with sliding block one (23), the lower end of hollow guide rod (24) is hollow shape；Motor one (21) by turntable (22), connecting rod and
Sliding block one (23) drives hollow guide rod (24) to be slided up and down in sliding slot two.
3. a kind of discrimination method of bacterium according to claim 2, it is characterised in that：The agitating unit (3) includes gas
Bottle (31), reagent bottle (32), tracheae (33) and automatic ventilation valve door (34) are provided with multiple bottle clips on the fixing body (1)
(14), and bottle clip (14) is located at cavity one (11), and bottle clip (14) is for being clamped reagent bottle (32)；The reagent bottle (32) includes
Bottle, rubber vent plug and syringe needle plug；The rubber vent plug is installed on bottle (321) and sealed bottle body (321), syringe needle plug are installed on
Bottle (321) lower end and sealed bottle body (321) lower end；The gas cylinder (31) is located at cavity one (11) top, and tracheae (33) is located at
Reagent bottle (32) upper end, automatic ventilation valve door (34) are located between gas cylinder (31) and tracheae (33), and automatic ventilation valve door (34)
One end and gas cylinder (31) unicom, the upper end unicom of automatic ventilation valve door (34) other end and tracheae (33)；The tracheae (33)
Rubber sleeve is arranged in lower end, and rubber sleeve is set in reagent bottle (32) upper end；It is provided with multiple reagent flows on the fixing body (1)
Road, one end of reagent runner are located at the underface of syringe needle plug, and what is be arranged on the other end of reagent runner and hollow guide rod (24) is logical
Hole unicom.
4. a kind of discrimination method of bacterium according to claim 3, it is characterised in that：Automatic ventilation valve door (34) packet
Controller (341), magnetic ball (342), electromagnet (343) and spring one are included, the controller (341) is located at fixing body (1) outside
On wall；It is provided with gas port at the cavity one (11)；The electromagnet (343) and spring one are located at gas port top, electromagnetism
It is provided with venthole on iron (343) and spring one, is connected by conducting wire between electromagnet (343) and spring one and controller (341)
It connects, electromagnet (343) and spring one make gas port be in aeration status for magnetic ball (342) to be sucked；The magnetic ball (342)
Positioned at gas port lower end, magnetic ball (342) is for blocking gas port；The spring one be located at electromagnet (343) and spring one with
Between magnetic ball (342) and spring one is used to magnetic ball (342) being pressed on gas port lower end；The upper end of the gas port and gas
Bottle (31) unicom, lower end and tracheae (33) unicom of gas port, and flowmeter is provided on gas-guide tube (33), flowmeter and control
Device (341) connects, and flowmeter is for detecting the gas flow for flowing through gas-guide tube (33).
5. a kind of discrimination method of bacterium according to claim 2, it is characterised in that：Reagent adding device (4) packet
Include shaft (41), torsional spring (42), elastic string one (43), elastic string two (44), resetting spring (45), the first axle sleeve (46), first
Axis (47), the second axis (48), thrust ball bearing (49) and sliding block two, the shaft (41) is located in cavity one (11), and shaft
(41) it is rotatablely connected with hollow guide rod (24)；One end of the torsional spring (42) is connect with shaft (41), the other end of torsional spring (42)
It is fixed on cavity one (11) inner wall；Sliding slot two is provided on the fixing body (1), sliding slot two is in 20 ° to 45 ° with vertical direction
Angle；First axle sleeve (46) is located on the same line with sliding slot one；The sliding block two is located in sliding slot two, and sliding block two
It is slidably connected with sliding slot two, one end of sliding block two is fixedly connected with the second axis (48) upper end, the other end and resetting spring of sliding block
(45) it connects, resetting spring (45) is located in sliding slot two；One end of the elastic string one (43) is fixed with cavity one (11) inner wall
Connection, the other end of elastic string one (43) are wrapped in shaft (41)；One end of the elastic string two (44) is fixed on shaft
(41) on, the other end of elastic string two (44) passes through resetting spring (45) and is fixed on sliding block two；The first axle (47) with
Thrust ball bearing (49) is provided between second axis (48), first axle (47) lower end is connect with thrust ball bearing (49) upper end, the
The upper end of two axis (48) is connect with the lower end of thrust ball bearing (49)；It is provided with the first spiral on the outer curved wall of the first axle (47)
Sliding slot, first axle (47) end are provided with agitator arm (471), and agitator arm (471) is used for stirring；First axle sleeve
(46) inner wall is equipped with the first bulb, and the first bulb is adapted with the first spiral chute, first axle (47) and the first axle sleeve
(46) rotational slide connects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810252824.6A CN108490198B (en) | 2018-03-26 | 2018-03-26 | Bacteria identification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810252824.6A CN108490198B (en) | 2018-03-26 | 2018-03-26 | Bacteria identification method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108490198A true CN108490198A (en) | 2018-09-04 |
| CN108490198B CN108490198B (en) | 2021-10-19 |
Family
ID=63337777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810252824.6A Active CN108490198B (en) | 2018-03-26 | 2018-03-26 | Bacteria identification method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108490198B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204302289U (en) * | 2014-12-12 | 2015-04-29 | 上海睿康生物科技有限公司 | A kind of glycated protein detection kit |
| CN204509337U (en) * | 2015-03-10 | 2015-07-29 | 李越 | A kind of bacteria culture bottle of adjustable pH value |
| CN105494292A (en) * | 2015-12-18 | 2016-04-20 | 山东农业大学 | Portable pesticide atomization machine |
| CN106319021A (en) * | 2016-08-30 | 2017-01-11 | 江门市凯林贸易有限公司 | Pretreatment method and equipment for bacterial specimens |
-
2018
- 2018-03-26 CN CN201810252824.6A patent/CN108490198B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204302289U (en) * | 2014-12-12 | 2015-04-29 | 上海睿康生物科技有限公司 | A kind of glycated protein detection kit |
| CN204509337U (en) * | 2015-03-10 | 2015-07-29 | 李越 | A kind of bacteria culture bottle of adjustable pH value |
| CN105494292A (en) * | 2015-12-18 | 2016-04-20 | 山东农业大学 | Portable pesticide atomization machine |
| CN106319021A (en) * | 2016-08-30 | 2017-01-11 | 江门市凯林贸易有限公司 | Pretreatment method and equipment for bacterial specimens |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108490198B (en) | 2021-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201449349U (en) | Automatic liquid based lamina cell smear machine | |
| CN106268459B (en) | A kind of shoes amylum adhesive raw materials for production mixing apparatus | |
| CN202471737U (en) | Discrete-type fully-automatic biochemical analyzer | |
| CN203941163U (en) | A kind of glycolated hemoglobin analysis liquid road | |
| CN102243234A (en) | Automatic bacterial sorting and labeling method based on immune method, and apparatus thereof | |
| CN108490198A (en) | A kind of discrimination method of bacterium | |
| CN201543626U (en) | Capillary tube cleaning device | |
| CN115253961A (en) | Underground water is agitating unit for analysis chemical examination | |
| CN208907865U (en) | A kind of environmental monitoring water-quality sampler | |
| CN104653426A (en) | High-precision plunger type trace sampling pump | |
| CN108441418A (en) | A kind of identification systems of bacterium | |
| CN109939636B (en) | Mixing device and method for test sample in analyzer reaction tank | |
| CN202177577U (en) | Sample introduction device for atomic fluorescence spectroscopy | |
| CN201618696U (en) | Reaction cup washing device for full-automatic immunity analyzer | |
| CN101126753A (en) | Method for in situ determination of root system respiration | |
| CN201903476U (en) | Bubble blending device | |
| CN103454135B (en) | Device for combining sample enrichment with analytical instrument | |
| CN203881786U (en) | Liquid sample injection system | |
| CN212341128U (en) | Liquid chromatograph sampling device | |
| CN208146014U (en) | A kind of high precision micro pipettor | |
| CN209508207U (en) | A kind of magnetic beads for purifying system of fed-batch test | |
| CN113663646A (en) | Preparation method and application of wide-spectrum specific antibody modified magnetic metal organic framework material | |
| CN216747064U (en) | Liquid sample mixing device | |
| CN113156051A (en) | Engineering geological exploration water quality analysis method | |
| CN112903993A (en) | Immunoassay kit for detecting aflatoxin B1 in grain and oil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210924 Address after: 276800 No. 37, Shanhai Road, Rizhao street, Donggang District, Rizhao City, Shandong Province Applicant after: Intel inspection and certification (Shandong) Co.,Ltd. Address before: Hunan Normal University, No.36 Lushan Road, Changsha, Hunan 410081 Applicant before: Guo Qing |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |