CN112903993A - Immunoassay kit for detecting aflatoxin B1 in grain and oil - Google Patents
Immunoassay kit for detecting aflatoxin B1 in grain and oil Download PDFInfo
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- CN112903993A CN112903993A CN202110198004.5A CN202110198004A CN112903993A CN 112903993 A CN112903993 A CN 112903993A CN 202110198004 A CN202110198004 A CN 202110198004A CN 112903993 A CN112903993 A CN 112903993A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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Abstract
The invention discloses an immunoassay kit for detecting aflatoxin B1 in grain and oil, which comprises a kit, a base and a reagent detection structure, wherein the reagent detection structure comprises an inlet structure, a buffer structure, an anti-blocking stirring structure, a blanking structure and a sampling structure, the inlet structure comprises an input pipeline, a control valve, a cover plate, an adding inlet and a magnetic suction block, a motor at the bottom of the anti-blocking stirring structure, a stirring shaft, an anti-blocking supporting seat, stirring fan blades and a brush plate, and the blanking control valve of the blanking structure, a placing motor, a placing groove, a placing seat, a bottom sliding plate, a bottom supporting plate and a bottom telescopic rod are arranged on the blanking structure; meanwhile, the stirring can be accelerated by using the stirring fan blades, so that the reaction speed is high; the device bottom is provided with the standing groove, can be convenient for carry out the unloading for the unloading is comparatively convenient.
Description
Technical Field
The invention relates to a kit, in particular to an immunoassay kit for detecting aflatoxin B1 in grain and oil.
Background
When aflatoxin B1 in grain and oil is detected, raw materials and reactants of a reaction kit need to be mixed, but reagents are usually directly poured into the raw materials for reaction in the mixing process, so that the reaction time is too long, and the reaction efficiency is not good.
Disclosure of Invention
The invention aims to provide an immunoassay kit for detecting aflatoxin B1 in grain and oil, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides an immunoassay kit for detecting aflatoxin B1 in grain and oil, includes kit, base and reagent detection structure, the kit bottom is fixed with the base, the inside reagent that is provided with of kit detects the structure, the reagent detects the structure including getting into structure, buffer structure, preventing stifled stirring structure, unloading structure and sampling structure, the kit top is provided with gets into the structure, it is provided with buffer structure to get into the structure output, the last stifled stirring structure that is provided with of buffer structure, the kit bottom is provided with unloading structure, the unloading structure side is provided with sampling structure.
As a further scheme of the invention: the entering structure comprises an input pipeline, a control valve, a cover plate, an adding inlet and a magnetic suction block, the input pipeline is communicated with the upper portion of the kit, the control valve is fixed to the middle position of the input pipeline, the adding box body is communicated with the upper portion of the input pipeline, the adding inlet is communicated with the top of the adding box body, the cover plate is rotatably arranged on the top of the adding inlet, and the cover plate and the connecting portion of the adding inlet are fixed with the magnetic suction block.
As a still further scheme of the invention: the buffer structure comprises a filter plate, and the filter plate is fixed in the middle of the inside of the kit.
As a still further scheme of the invention: prevent stifled stirring structure bottom motor, (mixing) shaft, prevent stifled supporting seat, stirring vane and brush board, kit bottom intermediate position is fixed with the bottom motor, bottom motor drive connects the (mixing) shaft, the (mixing) shaft intermediate position is fixed with prevents stifled supporting seat, prevent stifled supporting seat below and be fixed with the brush board, just (mixing) shaft and filter rotate and connect, the (mixing) shaft side is fixed with stirring vane just stirring vane sets up the filter below.
As a still further scheme of the invention: the blanking structure comprises a blanking control valve, a placing motor, a placing groove, a placing seat, a bottom sliding plate, a bottom supporting plate and a bottom telescopic rod, wherein the bottom supporting plate is fixed on the side edge of the base, the bottom telescopic rod is fixed above the bottom supporting plate, the bottom sliding plate is fixed at the end part of the bottom telescopic rod, the bottom sliding plate is connected with the base in a sliding mode, the placing motor is fixed on the bottom sliding plate, the placing motor drives the placing seat to be connected, the placing groove is formed in the upper portion of the placing seat, and a blanking port is formed in the bottom of the kit through the blanking control valve.
As a still further scheme of the invention: the sampling structure samples and places box, sampling pipeline and sampling control spare, kit below side UNICOM is provided with the sampling pipeline, be fixed with the sampling control spare on the sampling pipeline, the box is placed in the sampling pipeline output is provided with the sample, the pipeline of can stretching of sampling pipeline.
As a still further scheme of the invention: the side of the adding box body is provided with scale marks.
Compared with the prior art, the invention has the beneficial effects that: the anti-blocking device has the advantages that the filtering plate is arranged for buffering, and the brushing plate is used for brushing, so that the anti-blocking operation can be carried out while buffering; meanwhile, the stirring can be accelerated by using the stirring fan blades, so that the reaction speed is high; the device bottom is provided with the standing groove, can be convenient for carry out the unloading for the unloading is comparatively convenient.
Drawings
FIG. 1 is a schematic structural diagram of an immunoassay kit for detecting aflatoxin B1 in grain and oil.
FIG. 2 is a schematic diagram of the structure of the position of a placement groove in an immunoassay kit for detecting aflatoxin B1 in grain and oil.
FIG. 3 is a schematic diagram of a local enlarged structure of A in the immunoassay kit for detecting aflatoxin B1 in grain and oil.
FIG. 4 is a schematic diagram of the position structure of a brush plate in the immunoassay kit for detecting aflatoxin B1 in grain and oil.
In the figure: 1. a kit; 2. a blanking control valve; 3. a base; 4. a placing seat; 5. a bottom telescoping rod; 6. a bottom support plate; 7. placing a motor; 8. a placement groove; 9. a bottom sliding plate; 10. sampling and placing the box body; 11. a sampling pipe; 12. a sampling control member; 13. a bottom motor; 14. a guide plate; 15. a filter plate; 16. a stirring shaft; 17. scale lines; 18. an addition inlet; 19. an input pipe; 20. a control valve; 21. an anti-blocking supporting seat; 22. brushing the board; 23. a stirring fan blade; 24. an intermediate rotating shaft; 25. a magnetic block; 26. and (7) a cover plate.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific embodiments.
Example 1
Referring to fig. 1-4, an immunoassay kit for detecting aflatoxin B1 in grain and oil comprises a kit 1, a base 3 and a reagent detection structure, wherein the base 3 is fixed at the bottom of the kit 1, the reagent detection structure is arranged in the kit 1, the reagent detection structure comprises an inlet structure, a buffer structure, an anti-blocking stirring structure, a discharging structure and a sampling structure, the inlet structure is arranged at the top of the kit 1, the buffer structure is arranged at the output end of the inlet structure, the anti-blocking stirring structure is arranged on the buffer structure, the discharging structure is arranged at the bottom of the kit 1, and the sampling structure is arranged at the side edge of the discharging structure. The reagent feeding device comprises a feeding structure, a buffer structure, a sampling structure and a feeding structure, wherein the feeding structure is used for feeding a reaction reagent and a reactant, the buffer structure is used for buffering the reagent, the anti-blocking stirring structure is used for stirring the reagent and the reactant, the reactant and the reagent are fully reacted with a stock solution while the reactant is prevented from blocking the buffer structure, the sampling structure is used for sampling until the sampling structure is used for sampling, so that the reaction state and the generated result can be observed, the feeding structure is used for feeding and collecting the reacted liquid, and backup of a detection result is realized.
In the immunoassay kit for detecting aflatoxin B1 in grain and oil, the inlet structure comprises an input pipeline 19, a control valve 20, a cover plate 26, an adding inlet 18 and a magnetic block 25, the input pipeline 19 is communicated and arranged above the kit 1, the control valve 20 is fixed in the middle of the input pipeline 19, an adding box body is communicated and arranged above the input pipeline 19, the adding inlet 18 is communicated and arranged at the top of the adding box body, the cover plate 26 is rotatably arranged at the top of the adding inlet 18, and the magnetic block 25 is fixed at the joint of the cover plate 26 and the adding inlet 18. The cover plate 26 is first rotated to separate the magnet blocks 25 from each other, then reagent reactant is added from the position of the addition inlet 18, and then the cover plate 26 is closed to allow access to the inside of the reagent cartridge 1 by the input pipe 19.
In the immunoassay kit for detecting aflatoxin B1 in grain and oil, the buffer structure comprises a filter plate 15, and the filter plate 15 is fixed in the middle position in the kit 1. Buffering and filtration of the reactants is achieved by means of a filter plate 15.
In the above-mentioned detect aflatoxin B1's immunoassay kit in grain and oil, prevent stifled stirring structure bottom motor 13, (mixing) shaft 16, prevent stifled supporting seat 21, stirring fan 23 and brush board 22, 1 bottom intermediate position of kit is fixed with bottom motor 13, bottom motor 13 drive connection (mixing) shaft 16, 16 intermediate position of (mixing) shaft is fixed with prevents stifled supporting seat 21, prevent stifled supporting seat 21 below and be fixed with brush board 22, just (mixing) shaft 16 and filter 15 rotate to be connected, the (mixing) shaft 16 side is fixed with stirring fan 23 and stirring fan 23 sets up filter 15 below. Utilize bottom motor 13 to drive (mixing) shaft 16 for drive and prevent stifled supporting seat 21 and rotate, thereby make brush board 22 clean filter 15, avoid blockking up, drive stirring fan blade 23 simultaneously and rotate, make the realization stirring, reaction between the reactant is dissolved with higher speed.
In the above immunoassay kit for detecting aflatoxin B1 in grain and oil, blanking structure unloading control valve 2, placing motor 7, standing groove 8, placing seat 4, bottom sliding plate 9, bottom supporting plate 6 and bottom telescopic link 5, 3 sides of base are fixed with bottom supporting plate 6, bottom telescopic link 5 is fixed above bottom supporting plate 6, 5 end fixing of bottom telescopic link has bottom sliding plate 9, bottom sliding plate 9 and base 3 sliding connection, be fixed with placing motor 7 on the bottom sliding plate 9, placing motor 7 drive connection placing seat 4, placing seat 4 has been seted up to the top, the bottom of kit 1 is provided with the feed opening through unloading control valve 2. At first, utilize bottom telescopic link 5 to reduce for the height of bottom sliding plate 9 reduces, places the unloading bottle on standing groove 8, utilizes to place motor 7 afterwards and rotates middle pivot 24 and then drive and place seat 4 and rotate, thereby makes the unloading bottle place in unloading control valve 2 below, utilizes bottom telescopic link 5 extension afterwards, makes the unloading bottle realize the unloading in unloading control valve 2 below.
In the immunoassay kit for detecting aflatoxin B1 in grain and oil, the sampling structure is provided with a sampling placement box body 10, a sampling pipeline 11 and a sampling control part 12, the side edge of the lower part of the kit 1 is communicated with the sampling pipeline 11, the sampling control part 12 is fixed on the sampling pipeline 11, the sampling placement box body 10 is arranged at the output end of the sampling pipeline 11, and the sampling pipeline 11 is a stretchable pipeline. The sampling control part 12 is used for controlling the action of the sampling pipeline 11, so that the sampling of the sample is realized.
Example 2
Compared with embodiment 1, the improvement point of the present embodiment is that: the side of the adding box body is provided with scale marks 17. The mass of the reaction mass was observed using the scale 17.
The working principle of the invention is as follows: the reagent feeding device comprises a feeding structure, a buffer structure, a sampling structure and a feeding structure, wherein the feeding structure is used for feeding a reaction reagent and a reactant, the buffer structure is used for buffering the reagent, the anti-blocking stirring structure is used for stirring the reagent and the reactant, the reactant and the reagent are fully reacted with a stock solution while the reactant is prevented from blocking the buffer structure, the sampling structure is used for sampling until the sampling structure is used for sampling, so that the reaction state and the generated result can be observed, the feeding structure is used for feeding and collecting the reacted liquid, and backup of a detection result is realized.
The invention has the beneficial effects that: the anti-blocking device has the advantages that the filtering plate is arranged for buffering, and the brushing plate is used for brushing, so that the anti-blocking operation can be carried out while buffering; meanwhile, the stirring can be accelerated by using the stirring fan blades, so that the reaction speed is high; the device bottom is provided with the standing groove, can be convenient for carry out the unloading for the unloading is comparatively convenient.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (7)
1. The utility model provides an immunoassay kit for detecting aflatoxin B1 in grain and oil, includes kit (1), base (3) and reagent detection structure, its characterized in that, kit (1) bottom is fixed with base (3), kit (1) inside is provided with reagent detection structure, reagent detection structure is including getting into structure, buffer structure, preventing stifled stirring structure, blanking structure and sampling structure, kit (1) top is provided with and gets into the structure, it is provided with buffer structure to get into the structure output, the last stifled stirring structure that is provided with of buffer structure, kit (1) bottom is provided with blanking structure, the blanking structure side is provided with sampling structure.
2. The immunoassay kit for detecting aflatoxin B1 in grain and oil according to claim 1, wherein the entering structure comprises an input pipeline (19), a control valve (20), a cover plate (26), an adding inlet (18) and a magnetic block (25), the input pipeline (19) is communicated and arranged above the kit (1), the control valve (20) is fixed in the middle of the input pipeline (19), an adding box body is communicated and arranged above the input pipeline (19), the adding inlet (18) is communicated and arranged at the top of the adding box body, the cover plate (26) is rotatably arranged at the top of the adding inlet (18), and the magnetic block (25) is fixed at the joint of the cover plate (26) and the adding inlet (18).
3. The immunoassay kit for detecting aflatoxins B1 in grain and oil according to claim 2, wherein the buffer structure comprises a filter plate (15), and the filter plate (15) is fixed at an intermediate position inside the kit (1).
4. The immunoassay kit for detecting aflatoxin B1 in grain and oil according to claim 3, wherein the anti-blocking stirring structure comprises a bottom motor (13), a stirring shaft (16), an anti-blocking supporting seat (21), stirring blades (23) and a brush plate (22), the bottom motor (13) is fixed at the middle position of the bottom of the kit (1), the bottom motor (13) is in driving connection with the stirring shaft (16), the anti-blocking supporting seat (21) is fixed at the middle position of the stirring shaft (16), the brush plate (22) is fixed below the anti-blocking supporting seat (21), the stirring shaft (16) is rotatably connected with the filter plate (15), the stirring blades (23) are fixed at the side edge of the stirring shaft (16), and the stirring blades (23) are arranged below the filter plate (15).
5. The immunoassay kit for detecting aflatoxin B1 in grain and oil according to claim 4, it is characterized in that the blanking structure comprises a blanking control valve (2), a placing motor (7), a placing groove (8), a placing seat (4), a bottom sliding plate (9), a bottom supporting plate (6) and a bottom telescopic rod (5), a bottom supporting plate (6) is fixed on the side edge of the base (3), a bottom telescopic rod (5) is fixed above the bottom supporting plate (6), a bottom sliding plate (9) is fixed at the end part of the bottom telescopic rod (5), the bottom sliding plate (9) is connected with the base (3) in a sliding way, a placing motor (7) is fixed on the bottom sliding plate (9), the placing motor (7) is connected with the placing seat (4) in a driving way, a placing groove (8) is formed above the placing seat (4), and a feed opening is formed in the bottom of the kit (1) through a feed control valve (2).
6. The immunoassay kit for detecting aflatoxin B1 in grain and oil according to claim 5, wherein the sampling structure is provided with a sampling placement box body (10), a sampling pipeline (11) and a sampling control part (12), the side edge of the lower part of the kit (1) is communicated with the sampling pipeline (11), the sampling pipeline (11) is fixed with the sampling control part (12), the output end of the sampling pipeline (11) is provided with the sampling placement box body (10), and the sampling pipeline (11) is a stretchable pipeline.
7. The immunoassay kit for detecting aflatoxin B1 in grain and oil according to claim 6, wherein the side of the adding box body is provided with scale marks (17).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110198004.5A CN112903993B (en) | 2021-02-22 | 2021-02-22 | Immunoassay kit for detecting aflatoxin B1 in grain and oil |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110198004.5A CN112903993B (en) | 2021-02-22 | 2021-02-22 | Immunoassay kit for detecting aflatoxin B1 in grain and oil |
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| Publication Number | Publication Date |
|---|---|
| CN112903993A true CN112903993A (en) | 2021-06-04 |
| CN112903993B CN112903993B (en) | 2022-07-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110198004.5A Active CN112903993B (en) | 2021-02-22 | 2021-02-22 | Immunoassay kit for detecting aflatoxin B1 in grain and oil |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115389402A (en) * | 2022-10-26 | 2022-11-25 | 安徽佰欧晶医学科技有限公司 | Blood tumor determination kit and detection method |
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| CN112362607A (en) * | 2018-11-29 | 2021-02-12 | 平顶山市畜产品质量安全监测中心 | Detection cabinet for aflatoxin in corn feed stored in feedlot |
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2021
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Patent Citations (8)
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| CN108362527A (en) * | 2018-06-25 | 2018-08-03 | 湖南欧杰生物科技发展有限公司 | Three chamber communication type resampling filter devices and its water flowing filtering type sampling craft |
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| CN115389402A (en) * | 2022-10-26 | 2022-11-25 | 安徽佰欧晶医学科技有限公司 | Blood tumor determination kit and detection method |
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| CN112903993B (en) | 2022-07-22 |
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