CN109468221A - An indoor experimental device for simulating microbial carbonate rocks - Google Patents
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Abstract
本发明公开了一种室内模拟微生物碳酸盐岩的实验装置，包括进料机构及从左向右依次并排设置的第一培养单元、第二培养单元、第三培养单元和第四培养单元，每个培养单元均包括矿物储集机构及矿物筛选提取机构，矿物储集机构包括上矿物储集室和下矿物分离室，二者的相接区域设置有过滤网一和可抽拉档板，在矿物储集室上还安装有搅拌机构；第一培养单元和第二培养单元作为接入微生物的实验组，第三培养单元和第四培养单元作为接入与微生物同等体积蒸馏水的对照组。本发明实验装置能实现实验组和对照组在相同的条件下培养，能够快速观察实验组和对照组发生的不同反应现象。
The invention discloses an indoor experimental device for simulating microbial carbonate rock, comprising a feeding mechanism and a first culturing unit, a second culturing unit, a third culturing unit and a fourth culturing unit arranged side by side from left to right, Each culturing unit includes a mineral storage mechanism and a mineral screening and extraction mechanism. The mineral storage mechanism includes an upper mineral storage chamber and a lower mineral separation chamber. The connecting area between the two is provided with a filter screen and a pull-out baffle. A stirring mechanism is also installed on the mineral storage chamber; the first culture unit and the second culture unit are used as the experimental group with access to microorganisms, and the third culture unit and the fourth culture unit are used as the control group connected with the same volume of distilled water as the microorganisms. The experimental device of the invention can realize the cultivation of the experimental group and the control group under the same conditions, and can quickly observe the different reaction phenomena occurring in the experimental group and the control group.
Description
Technical field
The present invention relates to Microbial Carbonates mineral experimental provision technical fields, and in particular to a kind of micro- life of indoor simulation
The experimental provision of object carbonate rock.
Background technique
Microorganism induction carbonate mineral precipitating is one of research hotspot of geomicrobiology.Microorganism mainly passes through
Its metabolic activity promotes cell peripheral microenvironment pH value and water body [CO3 2-] raising, be eventually exhibited as carbonate mineral saturation
The increase of index, to be conducive to the precipitating of carbonate mineral.In the research of Microbial Carbonates at this stage, different Mg/Ca
Than, high salt concentration, microorganism seat etc. have important influence to mineral species, surface topography, element composition etc., and test
There is very big differences for object phase and pattern of group and control group Minerals etc..At present in the experiment of microorganism induction carbonate rock
Involved in experimental facilities it is fewer, device is more complicated, operate it is relatively difficult, simulating lab test contrast effect is unobvious.
The training method of microorganism induction carbonate rock is mainly concussion and cultivate case, culture bottle during shake culture at present
Easily inclination spills the culture medium come and easily corrodes instrument, not only maintenance cost because of the height of salinity needed for some bacteriums (Halophiles)
Height also reduces the service life of instrument；Integral asepsis condition is not achieved in control group, is easy microbiological contamination；Experimental group and control group cannot
Objective paired observation.The multi mineral of generation mixes, and not observes and analyzes.
Summary of the invention
Based on defect present in above-mentioned technology, the present invention provides a kind of experiment dress of indoor simulation Microbial Carbonates
It sets, which can study and simulate different Mg/Ca ratios, high salt concentration, separate sources Mg2+And the conditions pair such as various microorganisms
The effect and influence of carbonate mineral, can be in the pH value of same period measurement experiment group and control group culture medium, while side
Just the experiment simulation of Microbial Carbonates is carried out indoors.
The technical scheme adopted by the invention is that:
A kind of experimental provision of indoor simulation Microbial Carbonates, including feeding mechanism and successively set side by side from left to right
The first culture unit, the second culture unit, third culture unit and the 4th culture unit set, the first culture unit,
Feeding mechanism described in second culture unit, third culture unit and the 4th culture units shared；
The structure that described first cultivates unit, the second culture unit, third culture unit and the 4th culture unit is identical,
It include that mineral preserve mechanism and mineral screening extraction mechanism, it includes upper mineral reservoir chamber and lower mine that the mineral, which preserve mechanism,
Object separation chamber, the region that connects both in the upper mineral reservoir chamber and lower minerals separation room are provided with filter screen one and can
Pull plate washer, it is described can pull baffles be located at the lower section of the filter screen one, by the filter screen one and can pull baffles will
The inside of upper mineral reservoir chamber and lower minerals separation room is separated, and is also equipped with rabbling mechanism in the mineral reservoir chamber；
Experimental group of the first culture unit and the second culture unit as access microorganism, the third culture
The control group of unit and the 4th culture unit as access and the microorganism equal volume distilled water；
The mineral of the described first culture unit and the second culture unit preserve between mechanism, third culture unit and the 4th
The mineral of culture unit, which preserve, is respectively connected with pH analyzer between mechanism, by the pH analyzer that two adjacent cultures are single
Member is connected；
The lower section that the mineral of each culture unit preserve mechanism passes through A-frame and carries out firm support, in each triangle
Mineral described in being respectively provided with one group in the interior zone of the rack leg of bracket screen extraction mechanism, and the mineral screen extraction mechanism
Including the cylindrical mineral extraction chamber being located above and underlying cylindrical liquid collecting chamber, the mineral extraction chamber it is upper
Portion is connected by rotary switch with the lower minerals separation room, when needs are put into the mineral and solution of lower minerals separation room to mine
Object can open rotary switch when screening extraction mechanism, and the mineral of minerals separation room and solution is made to smoothly enter mineral extraction
In mechanism, it is additionally provided with filter screen two inside the mineral screening extraction mechanism, the filter screen two is small for obtaining
The mineral grain of partial size, the liquid collection chamber is for collecting liquid；
It is connected by pipeline between the feeding mechanism and each culture unit, is provided on the pipeline
Valve is controlled by related valve and is fed.
As a preferred solution of the present invention, opening is provided at the top of the mineral reservoir chamber, the opening is logical
It crosses rubber stopper to be sealed, osculum plug is provided on the rubber stopper, the osculum plug is for installing and the charger
The pipeline of structure connection.
As another preferred embodiment of the invention, gear is installed between the second culture unit and third culture unit
Plate is equipped with ultraviolet lamp on the baffle, is provided with hook on the right side of the baffle.
Further, rubber ring is equipped with below each A-frame, the bottom of the A-frame is provided with
Objective table.
Further, compressible screw thread rotation injection and suction port are additionally provided in the mineral reservoir chamber.
Compared with prior art, present invention offers following advantageous effects:
Experimental provision of the present invention, alkaline-resisting using salt tolerant, heat-resisting material is detachable, capable of washing, reusable, operation
It is convenient, simple, save the time；Good airproof performance prevents the unrestrained corrosion instrument of the culture medium of germ contamination and high salt concentration；Cost
It is low；Experimental group and control group are cultivated at identical conditions, are capable of the differential responses of rapid examination experimental group and control group generation
Phenomenon；A-frame adheres to rubber leather plug, and stability is high, is suitble to the popularization of Microbial Carbonates culture under any environment.
Detailed description of the invention
The present invention will be further explained below with reference to the attached drawings:
Fig. 1 is a kind of structural schematic diagram of the experimental provision of indoor simulation Microbial Carbonates of the present invention.
In figure, 1, material measure device；2, screw switch；3-1, rubber stopper；3-2, osculum plug；4-1, mineral reservoir chamber；4-
2, minerals separation room；5, blender；6, the switch of blender is controlled；7, compressible screw thread rotation injection and suction port；8, triangle
Bracket；9, objective table；10, pH analyzer；11, big partial size mineral filter screen (filter screen one) is screened；It 12, can pull baffles；13,
Rotary switch；14-1, mineral extraction element；14-2, fluid collection device；It 15, can pull filter screen (filter screen two)；16, spiral shell
Line rubber ring；17, baffle；18, it links up with；19, ultraviolet lamp；20, rubber ring.
Specific embodiment
The invention proposes a kind of experimental provision of indoor simulation Microbial Carbonates, in order to make advantages of the present invention,
Technical solution is clearer, clear, elaborates combined with specific embodiments below to the present invention.
As shown in Figure 1, a kind of experimental provision of indoor simulation Microbial Carbonates of the present invention, including from left to right successively
The first culture unit, the second culture unit, third culture unit, the 4th culture unit and the feeding mechanism being arranged, feeding mechanism
Positioned at the top, four culture one feeding mechanisms of units shared, feeding mechanism includes with graduated material measure device and rotation
Pass is turned on, material measure device ponderable quantity tests actually required solution, and rotary switch controls the channel of solution flow direction；Each culture is single
Member preserves mechanism including mineral and mineral screening extraction mechanism, mineral preserve mechanism and are divided into mineral reservoir chamber and lower mineral point
From room, the inside that mineral preserve mechanism is closed structure, and be internally provided with filter screen and can pull baffles, by filter screen and
Cavity is divided into upper mineral reservoir chamber and lower minerals separation room by baffle.Stirring easy to operate is installed in mineral reservoir chamber
Device, and the two groups of identical experimental groups in left and right pass through pH analyzer with control group device and are connected.PH analyzer is not only
The pH of the adjustable initial bacterial growth of solution and the situation of change that different time sections solution ph can also be measured.Bottom difference
It is provided with identical mineral extraction element, and stablizes the A-frame with rubber ring of whole device.In addition to this, entirely
Device can carry out sterilization processing in high-temperature sterilization pot and superclean bench, capable of washing, reusable.The experimental provision uses resistance to
Salt, alkaline-resisting, heat-resisting material, easy to operate, good airproof performance prevent the culture medium of germ contamination and high salt concentration from trickling down corrosion tester
Device is capable of the differential responses phenomenon of rapid examination experimental group and control group generation.
The experimental provision for simulating Microbial Carbonates indoors is equipped with removably with graduated material measure device, can
With solution needed for measuring experiment, operation is accurately and conveniently, dismountable when not used.The reality of Microbial Carbonates is simulated indoors
Experiment device has two groups of experimental groups and control group simultaneously, easily the differential responses phenomenon of both observations generation.In the two groups of mineral in left and right
Reservoir chamber is connected by pH analyzer, be provided with measurement pH value of solution electrode, can within the same period direct measurement experiment group
With the pH value of control group solution, prevent to pollute culture solution due to opening bottleneck in continuous mode leads to the entrance of other bacteriums；If
It is equipped with pH digital display screen, can intuitively see the pH value of solution.Connect with lower minerals separation room phase in mineral extraction element
Even, it is provided with filter screen, screens the mineral grain of different-grain diameter size, means of different convenient to use analyzes it；Setting
There are dismountable fluid collection vials, the liquid of collection can do the analysis such as ultra-thin section, EPS component and fluorescence intensity.It is stored up in mineral
Collection room is provided with compressible screw thread rotation injection and suction port, and one can be used for injecting different Mg/Ca ratios, sterile Na2CO3、
Sterile NaHCO3Deng solution needed for experiment, two can be used for drawing the measurement of the solution progress related experiment of reservoir chamber, and
Top is provided with 0.22 μm of filter membrane sealing, and leakproofness is good, and miscellaneous bacteria is not easily accessible reservoir chamber, prevents solution contaminated.It is propping up
The A-frame bottom that support mineral preserve device is provided with rubber stopper, anti-friction, can effectively play stable support function.It is located at
The ultraviolet lamp of control group can be killed effectively except bacterium, to prevent compareing for germ contamination access and microorganism equal volume distilled water
Group, to influence experimental result.
Preferably, above-mentioned experimental provision is equipped with with with opening, and Yi Genghuan battery and dismountable blender operate letter
Just.
Experimental provision structure and working principle of the invention is described briefly combined with specific embodiments below.
Embodiment 1:
Feed unit include material measure device 1, with the pipeline that connect of culture unit and the screw switch 2 on pipeline,
The culture medium that 150mL is prepared is measured by material measure device 1 and is added to mineral reservoir chamber 4-1, and wherein each flow direction of solution is logical
Road is controlled by screw switch 2, removes feeding device after the completion of addition, further includes that two groups of left and right is identical in addition to this
Mineral preserve device, mineral screening and extraction element, and two groups of the left side be the experimental group for accessing microorganism, two groups of the right for access and
The control group of microorganism equal volume distilled water.Wherein, it includes preserving for carrying out the mineral of mineral culture that mineral, which preserve device,
Room 4-1 and the minerals separation room 4-2, mineral reservoir chamber 4-1 and the minerals separation room 4-2 that are separated to mineral are by screening big mineral
The filter screen 11 of particle and can pull baffles 12 be split, filter screen 11 and baffle 12 can pulls.On mineral reservoir chamber 4-1
Rubber stopper 3-1 is installed, for sealing bottleneck, rubber stopper 3-1 is equipped with osculum plug 3-2, first is that it is used to assign feeding device, two
It can be used to assign ultraviolet lamp.In addition to this, the switch 6 that mineral reservoir chamber is equipped with detachable stirrer 5, controls blender
With the injection of compressible screw thread rotation and suction port 7, wherein blender 5 is used to agitating solution, is allowed to be contacted with air, open
It closes 6 to be used to control blender, with with opening, compressible screw thread rotation injection and suction port 7 can be used to injection culture mine
Calcium ions solution (CaCl needed for object process2Solution), (MgCl containing magnesium ion solution2·6H2O or MgSO4·7H2O)、
Na2CO3Solution and NaHCO3Solution, two can be used to draw a certain amount of solution in different time sections, to the characteristic of microorganism
It is analyzed, compressible function greatly saves space, and 0.22 μm of aseptic filter membrane being arranged on injection port can prevent miscellaneous
Bacterium foul solution.
The two groups of mineral in left and right preserve device and carry out firm support, triangle with the A-frame 8 of rubber ring 20 by bottom
Cylindrical mineral extraction element 14-1 and fluid collection device 14-2, the top of mineral extraction element 14-1 are provided among bracket
It is connected by rotary switch 13 with lower minerals separation room, when needs are put into the mineral and solution of lower minerals separation room to mineral and mention
Rotary switch can be opened by taking when device 14-1, the mineral of minerals separation room and solution is made to smoothly enter mineral extraction element
On 14-1, mineral extraction element 14-1 be internally provided with can pull filter screen 15, for obtaining the mineral grain of small particle, and
It is provided with screw thread rubber ring 16, for connecting mineral extraction element 14-1 and fluid collection device 14-2, fluid collection device 14-
2 are used to collect liquid, can be used as ultra-thin section, EPS component analysis and Fluorescence Intensity Assays etc., disassembly easy to install, while also easily
Cleaning.The two groups of mineral in left and right, which preserve device and are attached by pH analyzer 10, to be communicated, first is that the initial pH of adjustable solution,
Second is that the pH of different time sections solution can be measured.Marble objective table 9 is provided under A-frame, for carrying entire reality
Experiment device.In addition, being equipped with the baffle 17 of ultraviolet light between the two groups of devices in left and right, hook 18 is installed on the right side of baffle 17, hook 18 is used
To hang the ultraviolet lamp 19 that can be killed except miscellaneous bacteria.
The above entire experimental provision can sterilize in high-temperature sterilization pot and superclean bench, and partial devices are easy to install to tear open
It unloads, is easy cleaning, reusable, constant incubator can be directly placed at and cultivated, while time saving and energy saving, greatly saving
The space of concussion and cultivate case is saved.
It uses for reference the prior art and can be realized in the above-mentioned part that do not address.
It should be noted that any equivalent way that those skilled in the art are made under the introduction of this specification, or
Obvious variant should all be within the scope of the present invention.
Claims (5)
1. a kind of experimental provision of indoor simulation Microbial Carbonates, including feeding mechanism and successively it is arranged side by side from left to right
First culture unit, second culture unit, third culture unit and the 4th culture unit, it is characterised in that: described first
It cultivates unit, the second culture unit, third culture unit and the 4th and cultivates feeding mechanism described in units shared；
The structure that described first cultivates unit, the second culture unit, third culture unit and the 4th culture unit is identical, wraps
It includes mineral and preserves mechanism and mineral screening extraction mechanism, it includes upper mineral reservoir chamber and lower mineral point that the mineral, which preserve mechanism,
From room, the region that connects both in the upper mineral reservoir chamber and lower minerals separation room is provided with filter screen one and can pull
Plate washer, it is described can pull baffles be located at the lower section of the filter screen one, by the filter screen one and can pull baffles by upper mine
The inside of object reservoir chamber and lower minerals separation room is separated, and is also equipped with rabbling mechanism in the mineral reservoir chamber；
Experimental group of the first culture unit and the second culture unit as access microorganism, the third culture unit
Control group with the 4th culture unit as access and the microorganism equal volume distilled water；
The mineral of the first culture unit and the second culture unit preserve between mechanism, third culture unit and the 4th is cultivated
The mineral of unit, which preserve, is respectively connected with pH analyzer between mechanism, by the pH analyzer by two adjacent culture unit phases
Connection；
The lower section that the mineral of each culture unit preserve mechanism passes through A-frame and carries out firm support, in each A-frame
Rack leg interior zone in be respectively provided with one group described in mineral screening extraction mechanism, the described mineral screening extraction mechanism includes
The cylindrical mineral extraction chamber being located above and underlying cylindrical liquid collecting chamber, the top of the mineral extraction chamber by
Rotary switch is connected with the lower minerals separation room, is additionally provided with filter screen inside the mineral screening extraction mechanism
Two, the filter screen two is used to obtain the mineral grain of small particle, and the liquid collection chamber is for collecting liquid；
It is connected by pipeline between the feeding mechanism and each culture unit, is provided with valve on the pipeline
Door is controlled by related valve and is fed.
2. a kind of experimental provision of indoor simulation Microbial Carbonates according to claim 1, it is characterised in that: described
Mineral reservoir chamber at the top of be provided with opening, the opening is sealed by rubber stopper, is provided on the rubber stopper
Osculum plug, the osculum plug is for installing the pipeline connecting with the feeding mechanism.
3. a kind of experimental provision of indoor simulation Microbial Carbonates according to claim 1, it is characterised in that: described
Baffle is installed between second culture unit and third culture unit, ultraviolet lamp, the baffle are installed on the baffle
Right side is provided with hook.
4. a kind of experimental provision of indoor simulation Microbial Carbonates according to claim 1, it is characterised in that: every
Rubber ring is equipped with below a A-frame, the bottom of the A-frame is provided with objective table.
5. a kind of experimental provision of indoor simulation Microbial Carbonates according to claim 1, it is characterised in that: described
Compressible screw thread rotation injection and suction port are additionally provided in mineral reservoir chamber.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110964661A (en) * | 2019-11-13 | 2020-04-07 | 西南石油大学 | Method for simulating early degradation of organic matters in microbial rock |
| CN111855557A (en) * | 2020-07-20 | 2020-10-30 | 广东省微生物研究所（广东省微生物分析检测中心） | In-situ control experimental device and method for gas single-factor corrosion in composite corrosion factors |
| CN112322461A (en) * | 2020-10-29 | 2021-02-05 | 河北地质大学 | An indoor experimental device for simulating microbial carbonate rocks |
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| CN110964661A (en) * | 2019-11-13 | 2020-04-07 | 西南石油大学 | Method for simulating early degradation of organic matters in microbial rock |
| CN111855557A (en) * | 2020-07-20 | 2020-10-30 | 广东省微生物研究所（广东省微生物分析检测中心） | In-situ control experimental device and method for gas single-factor corrosion in composite corrosion factors |
| CN111855557B (en) * | 2020-07-20 | 2024-12-10 | 广东省微生物研究所（广东省微生物分析检测中心） | In-situ control experimental device and method for gas single factor corrosion in composite corrosion factors |
| CN112322461A (en) * | 2020-10-29 | 2021-02-05 | 河北地质大学 | An indoor experimental device for simulating microbial carbonate rocks |
| CN112322461B (en) * | 2020-10-29 | 2021-10-01 | 河北地质大学 | An indoor experimental device for simulating microbial carbonate rocks |
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