CN202730110U - Biochemical culturing and detecting device - Google Patents
Biochemical culturing and detecting device Download PDFInfo
- Publication number
- CN202730110U CN202730110U CN 201220376672 CN201220376672U CN202730110U CN 202730110 U CN202730110 U CN 202730110U CN 201220376672 CN201220376672 CN 201220376672 CN 201220376672 U CN201220376672 U CN 201220376672U CN 202730110 U CN202730110 U CN 202730110U
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- pilot trench
- cultivation
- cultivated
- culturing
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Abstract
The utility model relates to a biochemical culturing and detecting device and belongs to the field of microculture technology. The biochemical culturing and detecting device comprises a base plate (1) and a top cover (3) connected with the base plate (1) in a matched mode. The base plate (1) comprises a concave culturing pond (11) in the middle of the base plate (1), and a containing region (12) around the periphery of the culturing pond (11), and protrusions (122) of the containing region (12) are radially arranged around the culturing pond (11) and enable the containing region (12) to be divided into a plurality of fan-shaped storage locations. Culturing sub-grooves (2) are hollow wedge-shaped bodies, the narrow end of the culturing sub-grooves (2)faces towards the culturing pond (11) and are placed in the storage locations of the containing region (12), and each of the culturing sub-groove (2) is a fully-opened culturing sub-groove (2a) or a partly-opened culturing sub-groove (2b). The biochemical culturing and detecting device integrates enrichment culture, coating inoculation and biochemical detecting, the operation is flexible, requirements for the operating environment and detecting cost are reduced, and detecting efficiency and stability of results are improved.
Description
Technical field
The utility model relates to a kind of biochemical cultivation and proofing unit, belongs to microorganism culturing apparatus technical field.
Background technology
At present, in biomedicine detected and studies, Bacteria Detection was occupied significant proportion.How fast detect exactly the state, bacterium drug susceptibility of biology kind, the heritable variation of contained bacterium in the sample etc., become Interventions Requested indispensable in the routine work.
The API biochemical identification test kit of selling in the market is with complex operation, tediously long traditional method traceization and the stdn of explanation; With the result of biochemical reaction result in conjunction with other methods of inspection, in the database of processing through statistical study, compare, and then make kind and differentiate.But also there is following defective in API biochemical identification test kit:
1, API biochemical identification test kit can not be realized increasing the integrated of bacterium cultivation and biochemistry detection, need to increase first bacterium and cultivate, and then carry out a series of biochemical investigation in containing the tubule of reagent.Because the conversion of culture vessel and the design limitation of containing the plastics tubing of reagent need to just can operate under the higher environment of aseptic condition, otherwise may by the bacterial contamination in the external world, cause the impact on assay.
2, biochemical reaction test tubule small volume is given in the operation and is made troubles.
3, utilize API biochemical identification test kit to carry out Bacteria Identification before, need increase bacterium-separation and Culture, obtain single bacterium colony, and the substratum that contains certain nutritive ingredient is only suitable for the growth of some specific bacteria; If the contained bacterium of sample to be checked is unknown, then must adopt a plurality of culture dish, take up respectively the substratum that contains the Different Nutrition composition and carry out tentative test, to satisfy the needs of separation of bacterial; In addition, also need the bacterium of gained list bacterium colony is carried out some special checks, such as gram stain microscopy, oxidase test etc.; These are preconditions that institute must satisfy when using the API test kit, increased on the whole complicacy and the fussy degree of use API system, and whole flow process are consuming time longer.
4, API biochemical identification test kit still adopts manual inoculation, and efficient is low, is not suitable for the mass-producing operation.
5, use the cost of API biochemical identification test kit higher.
Summary of the invention
The purpose of this utility model is to overcome the deficiency of above-mentioned biochemical test device; provide a kind of energy will increase bacterium cultivation, inoculation, the integrated device of biochemistry detection; this installs, and inoculation, cultivation and detection method are simple, flexible operation; safe and reliable; improved the stability of detection efficiency and detected result; reduced requirement and testing cost to operating environment, be fit to mass-producing and use.
The purpose of this utility model is achieved in thatA kind of biochemical cultivation and proofing unit comprises the chassis and the top cover that are connected, and described chassis comprises and be arranged at the recessed cultivation pool of chassis central authorities and be centered around annular accommodating area around the cultivation pool,
At the bottom of described accommodating area comprises the inclination of tilting to cultivation pool, be arranged on several strip projected parts, internal perisporium and the periphery wall of end upper surface, turn up and be connected with the upper end of the internal perisporium of accommodating area in the upper edge of described cultivation pool, described projection is radial arrangement around cultivation pool, and the accommodating area is separated into several fan-shaped warehouse compartments;
Described chassis is provided with several and cultivates pilot trench, described cultivation pilot trench is hollow sphenoid, cultivate the narrow end of pilot trench towards cultivation pool, be shelved in the warehouse compartment of accommodating area, described cultivation pilot trench is that full open model is cultivated pilot trench or the open cultivation pilot trench of part, described full open model is cultivated the top of pilot trench and is not established cover plate, the top of the open cultivation pilot trench of described part is provided with a cover plate, described cover plate is trapezoidal, the length of described cover plate is less than the notch length of cultivating pilot trench, described cover plate is arranged on the narrow end of cultivating pilot trench, and is closed fixing with the upper edge of cultivating pilot trench.
The cross section on described chassis is circular or oval.
The upper edge of described cultivation pool is α with the angle of horizontal plane, and the scope of α is that 0 degree is to 90 degree.
The upper edge of described cultivation pool is α with the angle of horizontal plane, and the scope of α is that 20 degree are to 45 degree.
The number of the warehouse compartment of described accommodating area is 3～30.
Twice rib places, the outside of described cultivation pilot trench respectively are provided with half groove, a half-connection of half of described groove and projection.
Angle with horizontal plane at the bottom of the inclination of described accommodating area is β, and the scope of β is that 0 degree is to 90 degree.
Described chassis comprises several stiffening webs.
Described stiffening web is arranged on chassis downside and vertical chassis, and described stiffening web is radial arrangement around the outer wall of cultivation pool.
The lower end of the periphery wall of described accommodating area flushes with the bottom of cultivation pool to downward-extension.
The beneficial effects of the utility model are:
A kind of biochemical cultivation of the utility model is as follows with the characteristics of proofing unit:
(1) simultaneously receiving fluids, solid, semisolid medium, be about to various functions substratum such as enrichment medium, select substratum, detect substratum and concentrate in together;
(2) various substratum are separated from each other, and do not interfere with each other, and can be in contact with one another under certain physical condition, avoid the substratum of different phase mutually to mix detected result is caused interference, make detected result reliable and stable;
(3) bacterium is in the inner inoculation of device, namely need not open top cover from cultivation pool to the coating seeded process of cultivating pilot trench, inclination angle by modifier or carry out horizontal circumference and shake and finish the coating inoculation, reduced the false positive results that causes because of the environmental microorganism pollution, improved the stability of detection efficiency and detected result, also reduced the danger that strong pathogenic bacterium operation is brought, can make simultaneously and cultivate and the detection working scale;
(4) this device and cultivation thereof and detection method both can be used for the quick diagnosis detection of bacterium, the biological chemistry characteristics of observable bacterial enzyme system complete again, traditional identification by morphological characters, microbial culture, biochemical test concentrated in the device carry out synchronously, simple to operate, can greatly shorten time and efforts;
(5) this apparatus structure is simple, easy to operate, can improve detection efficiency, and it is detachable, separate to cultivate pilot trench, the handiness that has improved experimental implementation; Recycle on the chassis, has saved Financial cost.
Description of drawings
Fig. 1 is the assembling synoptic diagram of a kind of biochemical cultivation of the present invention and proofing unit (full open model cultivation pilot trench).
Fig. 2 is the schematic perspective view that chassis and full open model are cultivated pilot trench among Fig. 1.
Fig. 3 is the schematic perspective view on chassis among Fig. 2.
Fig. 4 is the schematic top plan view on chassis among Fig. 2.
Fig. 5 is the A-A cut-away view of Fig. 4.
Fig. 6 is the elevational schematic view on chassis among Fig. 2.
Fig. 7 is the schematic perspective view that full open model is cultivated the amplification of pilot trench among Fig. 1.
Fig. 8 is the schematic perspective view of the amplification of the open cultivation pilot trench of part.
Fig. 9 is the schematic perspective view of chassis and the open cultivation pilot trench of part.
Wherein:
Accommodating area 12
Tilt at the end 121
Periphery wall 124
Stiffening web 13
Cultivate pilot trench 2
Full open model is cultivated pilot trench 2a
The open cultivation pilot trench of part 2b
Embodiment
Referring to Fig. 1 to Fig. 9, a kind of biochemical cultivation and proofing unit of the utility model comprises the chassis 1 and the top cover 3 that are connected, and the cross section on described chassis 1 is circular or oval.Described chassis 1 comprises and is arranged at the recessed cultivation pool 11 of chassis 1 central authorities and is centered around annular accommodating area 12 around the cultivation pool 11.
Described accommodating area 12 comprises at the bottom of the inclination of tilting to cultivation pool 11 121, is arranged on several strip projected parts 122, internal perisporium 123 and the periphery wall 124 of the ends 121 upper surface.Turn up and be connected with the upper end of the internal perisporium 123 of accommodating area in the upper edge of described cultivation pool 11, the upper edge of described cultivation pool 11 is α with the angle of horizontal plane, and the scope of α is that 0 degree is to 90 degree.The discovery of research and test, the optimum range of α angle are that 20 degree are to 45 degree.The α angle on the chassis 1 shown in the figure is 25 degree.At the bottom of the inclination of described accommodating area 121 with the angle of horizontal plane be β, the scope of β angle is that 0 degree is to 90 degree.The β angle on the chassis 1 shown in the figure is 35 degree.Described protruding 122 center on cultivation pool 11 is radial arrangement, and accommodating area 12 is separated into several fan-shaped warehouse compartments.The number of the warehouse compartment of accommodating area 12 commonly used is 3～30.The warehouse compartment on the chassis 1 shown in the figure is 21.
Described chassis 1 also comprises several stiffening webs 13.Described stiffening web 13 is arranged on chassis 1 downside and vertical chassis 1, and described stiffening web 13 is radial arrangement around the outer wall of cultivation pool 11.The lower end of the periphery wall 124 of described accommodating area flushes to the bottom of downward-extension with cultivation pool 11.Are connected one and connect in described cultivation pool 11, accommodating area 12 with stiffening web.Twice rib places, the outside of described cultivation pilot trench 2 respectively are provided with half groove 22, a half-connection of half of described groove 22 and projection 122.Chassis 1 shown in the figure is provided with 21 warehouse compartments, and the number of the cultivation pilot trench 2 of its energy containing is no more than 21.
Described chassis 1 is provided with several and cultivates pilot trench 2, and described cultivation pilot trench 2 is hollow sphenoid, cultivates pilot trench 2 narrow ends towards cultivation pool 11, is shelved in the warehouse compartment of accommodating area 12.Described cultivation pilot trench 2 is cultivated pilot trench 2a or the open cultivation pilot trench of part 2b for full open model.Described full open model is cultivated the top of pilot trench 2a and is not established cover plate, and the top is fully open, as shown in Figure 7.The top of the open cultivation pilot trench of described part 2b is provided with a cover plate 21b, described cover plate 21b is trapezoidal, the length of described cover plate 21b is less than the notch length of cultivating pilot trench 2b, and described cover plate 21b is arranged on the narrow end of cultivating pilot trench 2b, and is closed fixing with the upper edge of cultivating pilot trench 2b.Such as Fig. 8, shown in Figure 9.
During use, cultivate pilot trench 2 and be placed on successively by the test design scheme on the warehouse compartment on apparatus of the present invention chassis 1, by protruding 122 screens.The liquid nutrient medium that contains detected sample is arranged on cultivation pool 11.Described liquid nutrient medium is one or more of enrichment medium of common enrichment medium, selective enrichment medium or specified strain; Or another kind of substratum, wherein can contain microbiotic, medicine, the compound of certain concentration known.Full open model is cultivated pilot trench 2a energy containing solid medium, semisolid medium etc. and is detected substratum, and the open cultivation pilot trench of part 2b energy containing liquid nutrient medium, solid or semisolid medium etc. detect substratum.Described detection substratum is one or more in selective medium, the biochemistry detection substratum; Or another kind of substratum, wherein can contain microbiotic, medicine, the compound of certain concentration known.
Detect substratum and be arranged on each cultivation pilot trench 2, inclination angle by modifier or carry out horizontal circumference and shake and the liquid nutrient medium that contains detected sample is got rid of respectively cultivate in the pilot trench 2, contact with the detection substratum in cultivating pilot trench 2, finish the coating inoculation, carry out again afterwards cultivation, observation, the comparison of microorganism, reach the purpose of biochemistry detection classification.
121 to cultivation pool 11 inclinations at the bottom of the inclination of described accommodating area, so that cultivation pilot trench 2 placed on it also tilts to cultivation pool 11.In detecting the full open model cultivation pilot trench 2a or the open cultivation pilot trench of part 2b that substratum is solid or semisolid medium, infiltrate the downslide of liquid nutrient medium under action of gravity of solid or semisolid medium, liquid nutrient medium has formed the mechanism of an automatic coating inoculation on solid or semisolid medium surface; Along with naturally absorption, the gravity settling of thalline, its bacterium colony density distribution gradient is larger the closer to cultivation pool 11 bacterium colony density.Can find with comparalive ease single bacterium colony to carry out morphologic observation, and in can the liquid body substratum in the microorganism unit volume how many impacts of quantity detect effects, improved stability and the reliability of detected result.And be in the open cultivation pilot trench of the part 2b of liquid nutrient medium detecting substratum, after getting rid of the liquid nutrient medium that contains detected sample, can be with the naked eye or observation of use instrument and detect muddy degree, the colour-change of above-mentioned mixed solution, or add detection reagent and further observe and detect.
Claims (10)
1. a biochemistry is cultivated and proofing unit, comprise the chassis (1) and the top cover (3) that are connected, it is characterized in that: described chassis (1) comprises and is arranged at the recessed cultivation pool (11) of chassis (1) central authorities and is centered around cultivation pool (11) annular accommodating area (12) all around
Described accommodating area (12) comprises (121) at the bottom of the inclination of tilting to cultivation pool (11), is arranged on several strip projected parts (122), internal perisporium (123) and the periphery wall (124) of the end (121) upper surface, turn up and be connected with the upper end of the internal perisporium (123) of accommodating area in the upper edge of described cultivation pool (11), described projection (122) is radial arrangement around cultivation pool (11), and accommodating area (12) are separated into several fan-shaped warehouse compartments;
Described chassis (1) is provided with several and cultivates pilot trench (2), described cultivation pilot trench (2) is hollow sphenoid, cultivate the narrow end of pilot trench (2) towards cultivation pool (11), be shelved in the warehouse compartment of accommodating area (12), described cultivation pilot trench (2) is cultivated pilot trench (2a) or the open cultivation pilot trench of part (2b) for full open model, described full open model is cultivated the top of pilot trench (2a) and is not established cover plate, the top of the open cultivation pilot trench of described part (2b) is provided with a cover plate (21b), described cover plate (21b) is trapezoidal, the length of described cover plate (21b) is less than the notch length of cultivating pilot trench (2b), described cover plate (21b) is arranged on the narrow end of cultivating pilot trench (2b), and is closed fixing with the upper edge of cultivating pilot trench (2b).
2. a kind of biochemistry according to claim 1 is cultivated and proofing unit, it is characterized in that: the cross section of described chassis (1) is for circular or oval.
3. a kind of biochemistry according to claim 1 is cultivated and proofing unit, and it is characterized in that: the upper edge of described cultivation pool (11) is α with the angle of horizontal plane, and the scope of α is that 0 degree is to 90 degree.
4. a kind of biochemistry according to claim 3 is cultivated and proofing unit, and it is characterized in that: the upper edge of described cultivation pool (11) is α with the angle of horizontal plane, and the scope of α is that 20 degree are to 45 degree.
5. a kind of biochemistry according to claim 1 is cultivated and proofing unit, and it is characterized in that: the number of the warehouse compartment of described accommodating area (12) is 3～30.
6. a kind of biochemistry according to claim 1 is cultivated and proofing unit, and it is characterized in that: twice rib places, the outside of described cultivation pilot trench (2) respectively are provided with half groove (22), a half-connection of half of described groove (22) and projection (122).
7. a kind of biochemistry according to claim 1 is cultivated and proofing unit, it is characterized in that: (121) are β with the angle of horizontal plane at the bottom of the inclination of described accommodating area, and the scope of β is that 0 degree is to 90 degree.
8. a kind of biochemistry according to claim 1 is cultivated and proofing unit, and it is characterized in that: described chassis (1) comprises several stiffening webs (13).
9. a kind of biochemistry according to claim 8 is cultivated and proofing unit, it is characterized in that: described stiffening web (13) is arranged on chassis (1) downside and vertical chassis (1), and described stiffening web (13) is radial arrangement around the outer wall of cultivation pool (11).
10. a kind of biochemistry according to claim 1 is cultivated and proofing unit, and it is characterized in that: the lower end of the periphery wall of described accommodating area (124) flushes to the bottom of downward-extension with cultivation pool (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220376672 CN202730110U (en) | 2012-08-01 | 2012-08-01 | Biochemical culturing and detecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220376672 CN202730110U (en) | 2012-08-01 | 2012-08-01 | Biochemical culturing and detecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202730110U true CN202730110U (en) | 2013-02-13 |
Family
ID=47655829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220376672 Withdrawn - After Issue CN202730110U (en) | 2012-08-01 | 2012-08-01 | Biochemical culturing and detecting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202730110U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816683A (en) * | 2012-08-01 | 2012-12-12 | 江苏嘉语生物医药技术有限公司 | Biochemical culture and detection device as well as detection method thereof |
-
2012
- 2012-08-01 CN CN 201220376672 patent/CN202730110U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816683A (en) * | 2012-08-01 | 2012-12-12 | 江苏嘉语生物医药技术有限公司 | Biochemical culture and detection device as well as detection method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20130213 Effective date of abandoning: 20131009 |
|
| RGAV | Abandon patent right to avoid regrant |