CN202730111U - Biochemical culturing and identifying device - Google Patents
Biochemical culturing and identifying device Download PDFInfo
- Publication number
- CN202730111U CN202730111U CN 201220376706 CN201220376706U CN202730111U CN 202730111 U CN202730111 U CN 202730111U CN 201220376706 CN201220376706 CN 201220376706 CN 201220376706 U CN201220376706 U CN 201220376706U CN 202730111 U CN202730111 U CN 202730111U
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- biochemical
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- cultivation
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Abstract
The utility model relates to a biochemical culturing and identifying device and belongs to the field of microculture technology. The biochemical culturing and identifying device comprises a base plate (1) and a top cover (3) connected with the base plate (1) in a matched mode. The base plate (1) comprises a culturing pond (11) and a containing region (12), wherein protrusions (122) of the containing region are radially arranged around the culturing pond (11) and the containing region (12) is divided into a plurality of fan-shaped storage locations. A primary culturing groove (2) is a hollow wedge-shaped body and the narrow end of the primary culturing groove (2) turns to the culturing pond (11) and is placed in the storage locations. A cover (21c) is arranged at the upper portion of a primary culturing groove (2c) and a liquid ingate (211), an air hole (212), a baffle plate I (213) and a baffle plate II (214) are arranged at the end close to the long side of the cover. The biochemical culturing and identifying device integrates enrichment culture, coating inoculation with biochemical identifying, requirement of the operating environment and detecting cost are reduced, and detecting efficiency and stability of results are improved.
Description
Technical field
The utility model relates to a kind of biochemical cultivation and the biochemical identification device, belongs to microorganism culturing apparatus technical field.
Background technology
At present, in biomedicine detects and studies, how fast to identify exactly the biology kind of microorganism contained in the sample, the state of heritable variation, become Interventions Requested indispensable in the routine work.
The API biochemical identification test kit of selling in the market gets up complex operation, tediously long traditional method traceization and the stdn of explanation with biochemical test reagent strip and database combination.But also there is following defective in API biochemical identification test kit:
1, API biochemical identification test kit can not be realized increasing integrated that bacterium is cultivated and biochemistry is differentiated, needs increase in the first liquid medium within bacterium and cultivates, and then carries out a series of biochemical investigation in the tubule that adds reagent strip.Because the conversion of culture vessel and the design limitation of reagent strip plastics tubing, need just can operate under the higher environment of aseptic condition, otherwise may by the fungi pollution in the external world, cause the impact on assay.
2, the substratum that contains certain nutritive ingredient is only suitable for the growth of some specific bacteria; If the contained bacterium of sample to be checked is unknown, then must adopt a plurality of culture dish, take up respectively the substratum that contains the Different Nutrition composition and carry out tentative test, to satisfy the needs of separation, discriminating bacteria.
3, API biochemical identification test kit still adopts line coating inoculation, and inoculation efficiency is low, is not suitable for the mass-producing operation.
4, use API biochemical identification test kit cost higher.
Summary of the invention
The purpose of this utility model is to overcome the deficiency of above-mentioned commercial reagent box, provide a kind of energy will increase bacterium and cultivate and the integrated biochemical identification device of biochemical discriminating, simple to operate, reduce the requirement to operating environment, improve detection efficiency and result's stability, reduce cost.
The purpose of this utility model is achieved in thatA kind of biochemical cultivation and the biochemical identification device comprises the chassis and the top cover that are connected, and described chassis comprises and be arranged at the recessed cultivation pool of chassis central authorities and be centered around annular accommodating area around the cultivation pool,
At the bottom of described accommodating area comprises the inclination of tilting to cultivation pool, be arranged on several strip projected parts, internal perisporium and the periphery wall of end upper surface, turn up and be connected with the upper end of the internal perisporium of accommodating area in the upper edge of described cultivation pool, described projection is radial arrangement around cultivation pool, and the accommodating area is separated into several fan-shaped warehouse compartments;
Described chassis is provided with several and cultivates pilot trench, described cultivation pilot trench is hollow sphenoid, cultivate the narrow end of pilot trench towards cultivation pool, be shelved in the warehouse compartment of accommodating area, the top of cultivating pilot trench is provided with cover plate, described cover plate is trapezoidal, the length of described cover plate and the notch equal in length of cultivating pilot trench, described cover plate is closed fixing with the upper edge of cultivating pilot trench, one end on the long limit of close cover plate is provided with the hole, liquid inlet, pore, shutter I and shutter II, described pore is located between the long limit of hole, liquid inlet and cover plate, the curved T letter stencil of described shutter I, the transverse part of T letter stencil is located on the cover plate, and the vertical part of T letter stencil is located under the cover plate, the curved cross bar of described shutter II, and be located under the cover plate, the nock of described shutter I is towards the long limit of cover plate, and the nock of described shutter II is towards the minor face of cover plate, described shutter I is not all touched the bottom of cultivating pilot trench with the vertical cover plate of shutter II and lower end, and the inner arc of hole, described liquid inlet and shutter I is tangent.
Hole, described liquid inlet is slotted eye or circular port.
The number in hole, described liquid inlet is one or more.
The number of described pore is one or more.
Twice rib places, the outside of described cultivation pilot trench respectively are provided with half groove, a half-connection of half of described groove and projection.
The lower end of the periphery wall of described accommodating area flushes with the bottom of cultivation pool to downward-extension.
The cross section on described chassis is circular or oval.
Described chassis comprises several stiffening webs.
Described stiffening web is arranged on chassis downside and vertical chassis, and described stiffening web is radial arrangement around the outer wall of cultivation pool.
The number of the warehouse compartment of described accommodating area is 3～30.
The beneficial effects of the utility model are:
A kind of biochemical cultivation of the utility model is as follows with the characteristics of proofing unit:
(1) various substratum are separated from each other, and do not interfere with each other, and can be in contact with one another under certain physical condition, have avoided mutually mixing the interference to detected result that causes between the different culture media, make detected result reliable and stable.
(2) bacterium namely need not opened top cover from cultivation pool to the process of cultivating pilot trench in the inner inoculation of device, inclination angle that can be by modifier or carry out horizontal circumference and shake coating and finish inoculation; Reduced because environmental microorganism pollutes the false positive results that causes, improved the stability of detection efficiency and detected result; Can make simultaneously cultivation and detection carry out the mass-producing operation carries out.
(3) substratum of the various functions of the cultivation pilot trench of this device energy containing such as enrichment medium, selection substratum, differential medium, they are concentrated in the device, this device and cultivation thereof and detection method both can be used for the quick diagnosis of bacterium and had differentiated, the biological chemistry characteristics of observable bacterial enzyme system complete again, traditional identification by morphological characters, method for cultivation of bacteria and biochemical test be accommodated in the device carry out synchronously, simple to operate, can greatly shorten required time and efforts.
(4) simple, the complete function of this apparatus structure has been saved Financial cost.
Description of drawings
Fig. 1 is the assembling synoptic diagram of a kind of biochemical cultivation of the utility model and biochemical identification device.
Fig. 2 is chassis and the schematic perspective view of cultivating pilot trench among Fig. 1.
Fig. 3 is the schematic perspective view on chassis among Fig. 2.
Fig. 4 is the schematic top plan view on chassis among Fig. 3.
Fig. 5 is the A-A cut-away view of Fig. 4.
Fig. 6 is the elevational schematic view on chassis among Fig. 3.
Fig. 7 is the schematic perspective view of cultivating the amplification of pilot trench among Fig. 1.
Wherein:
Accommodating area 12
Tilt at the end 121
Projection 122
Internal perisporium 123
Periphery wall 124
Stiffening web 13
Cultivate pilot trench 2c
Hole, liquid inlet 211
Shutter I 213
Shutter II 214
Embodiment
Referring to Fig. 1 to Fig. 7, a kind of biochemical cultivation and the biochemical identification device of the utility model comprises the chassis 1 and the top cover 3 that are connected, and the cross section on described chassis 1 is circular or oval.The cross section on the chassis 1 among the figure is circular.
Described chassis 1 comprises and is arranged at the recessed cultivation pool 11 of chassis 1 central authorities, is centered around annular accommodating area 12 and several stiffening webs 13 around the cultivation pool 11.Described stiffening web 13 is arranged on chassis 1 downside and vertical chassis 1, and described stiffening web 13 is radial arrangement around the outer wall of cultivation pool 11.
Described accommodating area 12 comprises at the bottom of the inclination of tilting to cultivation pool 11 121, is arranged on several strip projected parts 122, internal perisporium 123 and the periphery wall 124 of the ends 121 upper surface, turn up and be connected with the upper end of the internal perisporium 123 of accommodating area in the upper edge of described cultivation pool 11, the upper edge of described cultivation pool 11 is α with the angle of horizontal plane, and the scope of α is that 0 degree is to 90 degree.The discovery of research and test, the optimum range of α angle are that 20 degree are to 45 degree.The α on the chassis 1 shown in the figure is 25 degree.At the bottom of the inclination of described accommodating area 121 with the angle of horizontal plane be β, the scope of β is that 0 degree is to 90 degree.The β on the chassis 1 shown in the figure is 35 degree.Described protruding 122 center on cultivation pool 11 is radial arrangement, and accommodating area 12 is separated into several fan-shaped warehouse compartments.The number of the warehouse compartment of accommodating area 12 commonly used is 3～30.The warehouse compartment on the chassis 1 shown in the figure is 21.
The lower end of the periphery wall 124 of described accommodating area flushes to the bottom of downward-extension with cultivation pool 11.
Described chassis 1 is provided with several and cultivates pilot trench 2c, and described cultivation pilot trench 2c is hollow sphenoid, cultivates the narrow end of pilot trench 2c towards cultivation pool 11, is shelved in the warehouse compartment of accommodating area 12.Twice rib places, the outside of described cultivation pilot trench 2c respectively are provided with half groove 22, a half-connection of half of described groove 22 and projection 122.
The top of cultivating pilot trench 2c is provided with cover plate 21c, and described cover plate 21c is trapezoidal, the length of described cover plate 21c and the notch equal in length of cultivating pilot trench 2c, and described cover plate 21c is upper along closed fixing with cultivation pilot trench 2c's.One end on the long limit of close cover plate 21c is provided with hole, liquid inlet 211, pore 212, shutter I 213 and shutter II 214, and hole, described liquid inlet 211 is slotted eye or circular port.The number in hole, described liquid inlet 211 is one or more.
Described pore 212 is located between the long limit of hole, liquid inlet 211 and cover plate 21c, and the number of described pore 212 is one or more.
Described shutter I 213 curved T letter stencils, the transverse part of T letter stencil is located on the cover plate 21c, and the vertical part of T letter stencil is located under the cover plate 21c.Described shutter II 214 curved cross bars, and be located under the cover plate 21c, the nock of described shutter I 213 is towards the long limit of cover plate 21c, and the nock of described shutter II 214 is towards the minor face of cover plate 21c.Described shutter I 213 is not all touched the bottom of cultivating pilot trench 2c with shutter II 214 vertical cover plate 21c and lower end.Hole, described liquid inlet 211 is tangent with the inner arc of shutter I 213.
During use, containing contains the liquid nutrient medium of sample to be detected in the cultivation pool 11, and described liquid nutrient medium is one or more of enrichment medium of common enrichment medium, selective enrichment medium or specified strain; Or another kind of substratum, wherein can contain microbiotic, medicine, the compound of certain concentration known.Cultivation pilot trench 2c containing detects and uses liquid nutrient medium, described detection substratum is one or more in selective medium, the biochemical differential medium; Or another kind of substratum, wherein can contain microbiotic, medicine, the compound of certain concentration known.Each is cultivated pilot trench 2c be shelved on successively in the warehouse compartment of accommodating area 12, by protruding 122 screens; Inclination angle by modifier or carry out horizontal circumference and shake and the liquid nutrient medium that contains detected sample is got rid of respectively cultivate among the pilot trench 2c makes the liquid nutrient medium that contains detected sample contact with liquid nutrient medium with detecting, and finishes inoculation, cultivates again.Can be with the naked eye or observation of use instrument and detect muddy degree, the colour-change of above-mentioned mixed solution, or add detection reagent and further observe and detect, the biochemical purpose of differentiating finished.
121 to cultivation pool 11 inclinations at the bottom of the inclination of described accommodating area, so that cultivation pilot trench 2c placed on it also tilts to cultivation pool 11.This device is when shaking, and the liquid nutrient medium in the cultivation pool 11 is got rid of respectively cultivates pilot trench 2c, collects through the transverse part cambered surface of shutter I 213, and liquid nutrient medium enters through hole, liquid inlet 211 cultivates pilot trench 2c.The variation of described shutter I 213 sizes can cause the change of feed liquor amount.Hole, described liquid inlet 211 and described shutter I 213 cooperatively interact, the common volume that determines to enter cultivation pilot trench 2c liquid.Pore 212 has guaranteed the unobstructed of air-flow, has avoided the generation of bubble.Simultaneously the liquid nutrient medium in cultivating pilot trench 2c through the vertical part of shutter I 213 and shutter II 214 block effect after, can prevent liquid nutrient medium in the process of shaking from hole, liquid inlet 211 or pore 212 spill and cultivate pilot trench 2c.
Claims (10)
1. a biochemistry is cultivated and the biochemical identification device, comprise the chassis (1) and the top cover (3) that are connected, it is characterized in that: described chassis (1) comprises and is arranged at the recessed cultivation pool (11) of chassis (1) central authorities and is centered around cultivation pool (11) annular accommodating area (12) all around
Described accommodating area (12) comprises (121) at the bottom of the inclination of tilting to cultivation pool (11), is arranged on several strip projected parts (122), internal perisporium (123) and the periphery wall (124) of the end (121) upper surface, turn up and be connected with the upper end of the internal perisporium (123) of accommodating area in the upper edge of described cultivation pool (11), described projection (122) is radial arrangement around cultivation pool (11), and accommodating area (12) are separated into several fan-shaped warehouse compartments;
Described chassis (1) is provided with several and cultivates pilot trench (2c), described cultivation pilot trench (2c) is hollow sphenoid, cultivate the narrow end of pilot trench (2c) towards cultivation pool (11), be shelved in the warehouse compartment of accommodating area (12), the top of cultivating pilot trench (2c) is provided with cover plate (21c), described cover plate (21c) is trapezoidal, the length of described cover plate (21c) and the notch equal in length of cultivating pilot trench (2c), described cover plate (21c) is closed fixing with the upper edge of cultivating pilot trench (2c), one end on the long limit of close cover plate (21c) is provided with hole, liquid inlet (211), pore (212), shutter I (213) and shutter II (214), described pore (212) is located between the long limit of hole, liquid inlet (211) and cover plate (21c), the curved T letter stencil of described shutter I (213), the transverse part of T letter stencil is located on the cover plate (21c), the vertical part of T letter stencil is located under the cover plate (21c), the curved cross bar of described shutter II (214), and be located under the cover plate (21c), the nock of described shutter I (213) is towards the long limit of cover plate (21c), the nock of described shutter II (214) is towards the minor face of cover plate (21c), described shutter I (213) is not all touched the bottom of cultivating pilot trench (2c) with the vertical cover plate of shutter II (214) (21c) and lower end, and hole, described liquid inlet (211) is tangent with the inner arc of shutter I (213).
2. a kind of biochemical cultivation according to claim 1 and biochemical identification device, it is characterized in that: hole, described liquid inlet (211) is slotted eye or circular port.
3. a kind of biochemical cultivation according to claim 1 and biochemical identification device, it is characterized in that: the number in hole, described liquid inlet (211) is one or more.
4. a kind of biochemical cultivation according to claim 1 and biochemical identification device, it is characterized in that: the number of described pore (212) is one or more.
5. a kind of biochemical cultivation according to claim 1 and biochemical identification device, it is characterized in that: twice rib places, the outside of described cultivation pilot trench (2c) respectively are provided with half groove (22), a half-connection of half of described groove (22) and projection (122).
6. a kind of biochemical cultivation according to claim 1 and biochemical identification device, it is characterized in that: the lower end of the periphery wall of described accommodating area (124) flushes to the bottom of downward-extension with cultivation pool (11).
7. a kind of biochemical cultivation according to claim 1 and biochemical identification device, it is characterized in that: the cross section of described chassis (1) is for circular or oval.
8. a kind of biochemical cultivation according to claim 1 and biochemical identification device, it is characterized in that: described chassis (1) comprises several stiffening webs (13).
9. a kind of biochemical cultivation according to claim 8 and biochemical identification device, it is characterized in that: described stiffening web (13) is arranged on chassis (1) downside and vertical chassis (1), and described stiffening web (13) is radial arrangement around the outer wall of cultivation pool (11).
10. a kind of biochemical cultivation according to claim 1 and biochemical identification device, it is characterized in that: the number of the warehouse compartment of described accommodating area (12) is 3～30.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220376706 CN202730111U (en) | 2012-08-01 | 2012-08-01 | Biochemical culturing and identifying device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220376706 CN202730111U (en) | 2012-08-01 | 2012-08-01 | Biochemical culturing and identifying device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202730111U true CN202730111U (en) | 2013-02-13 |
Family
ID=47655830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220376706 Expired - Fee Related CN202730111U (en) | 2012-08-01 | 2012-08-01 | Biochemical culturing and identifying device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202730111U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110093273A (en) * | 2019-06-13 | 2019-08-06 | 中国科学院苏州生物医学工程技术研究所 | Culture module is imaged in embryo's time difference |
| US11496057B2 (en) | 2019-02-01 | 2022-11-08 | Delta Electronics, Inc. | Converter for improving conversion efficiency |
-
2012
- 2012-08-01 CN CN 201220376706 patent/CN202730111U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11496057B2 (en) | 2019-02-01 | 2022-11-08 | Delta Electronics, Inc. | Converter for improving conversion efficiency |
| CN110093273A (en) * | 2019-06-13 | 2019-08-06 | 中国科学院苏州生物医学工程技术研究所 | Culture module is imaged in embryo's time difference |
| CN110093273B (en) * | 2019-06-13 | 2023-12-05 | 济南国科医工科技发展有限公司 | Embryo time difference imaging culture module |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130213 Termination date: 20150801 |
|
| EXPY | Termination of patent right or utility model |