CN2478109Y - Immunity pick-up plate - Google Patents
Immunity pick-up plate Download PDFInfo
- Publication number
- CN2478109Y CN2478109Y CN 01207812 CN01207812U CN2478109Y CN 2478109 Y CN2478109 Y CN 2478109Y CN 01207812 CN01207812 CN 01207812 CN 01207812 U CN01207812 U CN 01207812U CN 2478109 Y CN2478109 Y CN 2478109Y
- Authority
- CN
- China
- Prior art keywords
- micropore
- plate
- plate body
- microflute
- immunity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an immunity detection plate used in the antigen (or antibody) immunologic diagnosis approach, namely the immunity micro-bore array detection. The technical proposal is that a micro-flute 3 is provided between a micro-bore 2 on a plate body 1; the distribution of micro-bore is high density; when the micro-flute is not provided, a cover 4 is provided on the plate body 1, and a closed space with micro-gap is provided between the cover 4 and the plate body 1; the array of the micro-bore 2 is group type, and the upper surface of each group of micro-bore is lower than the upper surface of the plate body 1 to form a reaction tank 5.A plurality of pathogenic factors such as antigen (or antibody) can be checked and analyzed simultaneously by using the plate. This practical utility model has the advantages that the performance is stable and reliable, operation is simple, and efficiency is high.
Description
The utility model relates to employed a kind of plate for detecting immunity in antigen (or antibody) immune diagnostic method-immune microwell array detection.
Occurring in nature can be caused by multiple paathogenic factor with a kind of disease.But in diagnosis, a common experimental system can only detect a kind of antigen or antibody, and quantity of information is little, therefore, even a kind of disease also must detect respectively with multiple antigen-antibody detection system sometimes.Yet, when detecting a kind of antigen or antibody, can only prove that this antigen or antibody are relevant with this disease, but can not get rid of the existence of other relevant paathogenic factor.Particularly when obtaining negative result, the doctor more can't judge this disease for due to which kind of paathogenic factor, and can't determine therapeutic scheme.In order to clarify a diagnosis, also must do further inspection up to can making a definite diagnosis, and delay treatment.Patient Ze Yin can not get in time, effectively treatment, and incur loss through delay or the treatment opportunity of letting sth. slip by, and regret to the very end of one's life, even loss of life.Yet, the microwell plate that is used for immune detection that can see in the market, general overall dimensions are big (13 * 8.5 centimetres); Pore density is lower, as 96 holes or 384 orifice plates; Micropore does not link to each other mutually, and the sidewall height, and is general＞1 centimetre, therefore must clean, drip detected sample, developer or label etc. respectively; Adsorption capacity is little or adsorptive power is low.Thereby still there is not at present an ideal product that can be used for immune microwell array check and analysis.
The immunity microarray detection processes is a kind of brand-new detection method, it adopts special-purpose microwell plate to carry out biomedical detections such as immunity, have on this microwell plate several, tens, even several ten thousand or higher quantity, absorption or solidified (or claim bag by) antigen (or antibody) respectively, and in a certain order or form, high density row be listed in immune micropore together; In testing process, can once obtain the testing result of all known antigens (or antibody) in each hole of microwell array.Adopt the biomedical technology of this high flux biological information, once can to several, tens kinds, even the antigen paathogenic factors such as (or antibody) of several ten thousand kinds or higher quantity is carried out check and analysis simultaneously.Thereby one-time detection can obtain the pathogenic information of several even up to ten thousand kinds of diseases simultaneously.It is very big, accurate, sensitive that immunity microwell array detection method has quantity of information, fast, in time, good reproducibility, good reliability and easy and simple to handle and advantage such as automaticity is high, production cost is low.
The purpose of this utility model exactly will be for immune microwell array detection technique designs a kind of special immune check-out console, characteristics such as that this check-out console in use should have is easy and simple to handle, stable and reliable for performance, practicality.
In order to reach the concrete technical scheme that the purpose of this utility model takes be, be provided with microflute 3 between the micropore on the plate body 12, microflute 3 and micropore 2 form chains;
The degree of depth of micropore 2 is the 0-2 millimeter, when the degree of depth of micropore near zero the time, micropore be distributed as high density;
The width of microflute 3 can equate with the aperture of micropore 2, promptly only occurs the microflute that links up on plate body;
Be furnished with on plate body 1 and cover 4, lid 4 forms confined space with micropore 2 and microflute 3;
The arrangement of micropore 2 can have multiple mode, can be as a whole, and grouping or evenly distributed also can be the assembly that tape frame is removable or porous is removable;
Plate for detecting immunity can not have microflute 3 yet, it is characterized in that: be furnished with on the plate body 1 and cover 4, form the confined space of microgap 6 between lid 4 and the plate body 1;
The plate for detecting immunity that does not have microflute 3 also can be this form: micropore 2 be arranged as packet type, the last plane of every group of micropore is lower than the last plane of plate body 1 and forms reaction tank 5;
In order to increase absorption affinity, the bottom and the sidewall of micropore 2, microflute 3 can be rough and uneven in surface shape, as zigzag, cone-shaped.
The material of plate body 1 is transparent or opaque.
The utility model plate for detecting immunity has reliable and stable, easy and simple to handle, the advantages such as efficient is high, practicality of usability as a kind of support equipment of brand-new detection method.
Fig. 1 is the vertical view of one of embodiment of the utility model plate for detecting immunity;
Fig. 2 is the A-A cut-open view of Fig. 1;
Fig. 3 is two the vertical view of the embodiment of the utility model plate for detecting immunity;
Fig. 4 is three the vertical view of the embodiment of the utility model plate for detecting immunity;
Fig. 5 is the A-A cut-open view of Fig. 4;
Fig. 6 is four the vertical view of the embodiment of the utility model plate for detecting immunity;
The A-A cut-open view of Fig. 7 Fig. 6;
Fig. 8 is five the cut-open view of the embodiment of the utility model plate for detecting immunity.
Below in conjunction with accompanying drawing the utility model is further described.
As shown in the figure, 1 is plate body, and 2 is micropore, and 3 is microflute, and 4 are lid, and 5 is that reaction tank, 6 is microgap.
The utility model plate for detecting immunity, compare with existing microwell plate, have following characteristics in design: 1) the microflute (see figure 1) that links to each other is arranged between the hole, help the continuous flow of various liquid (as detected sample, label or washing lotion etc.) between each micropore, reduce the consumption of various liquid, and simplify the operation therefrom.Sample flow is when each hole, detected material can combine with the solid phase homologue that adsorbed in the micropore, can cause the constantly diluted influence that produces of detected material owing to solved the liquid part continuous balance again in whole liquid phase that combines with solid phase because of detected material, therefore detected material can be in the relative high concentration of the local formation of reacting hole, promote the formation of bond, and detection sensitivity is improved; 2) on plate body, carve continuous, (aperture equates with groove width high-density, trench, see Fig. 3), the utilization specialized instrument and equipment can be added in the bioactive molecule of different qualities (as antigen, antibody, nucleic acid molecules, enzyme or/and hormone etc.) formation microarray in the microchannels in regular turn, be used for immune microwell array check and analysis, also can reach purpose and the effect same with characteristics 1.3) lid.Shape can be varied, usually add a cover is for the ease of preservation and the cleaning that keeps the micropore frid, but, when adding a cover for this plate for detecting immunity, has following very special significance: the pipeline that A. cover plate or shell interconnect the airtight Kong Yukong of becoming of microflute, then liquid is under certain pressure, flow and don't overflow hole slot along certain direction, must clean, drip problems such as inconvenience that detected sample and label etc. are brought and sensitivity reduction respectively to solve each micropore of common microwell plate; When B. on the shallow bore hole microwell plate of no microflute, adding a cover, between lid and microwell plate in several ways (as adding ring liner, projection) form the microgap (see figure 8), so that sample to be checked, label or washing lotion etc. flow in each hole of microwell plate by overflowing, the purpose of reacting, clean, adding label or developer to reach; When C. being divided into a plurality of micropore group on a microwell plate, microwell plate can uncovered, slotless, so that a plurality of sample to be checked carries out parallel check and analysis (as Fig. 6, Fig. 7) to multiple different factors simultaneously; 4) shallow bore hole.Therefore the degree of depth 0-2 millimeter of micropore helps simplifying the operation, and improves sensitivity, reduces amount of samples (referring to the 1-3 bar); 5) this plate for detecting immunity external form is generally less, has only standard carrier sheet size (25 * 75 millimeters) or about 1 square centimeter, even littler; 6) pore density of this plate for detecting immunity is higher, and the number cells of the high density microwell plate of a general standard carrier sheet size is no less than 6, can reach dozens of in addition tens thousand of more than; 7) arrangement of micropore can have multiple mode, can be as a whole, and grouping or evenly distributed (seeing Fig. 4, Fig. 6) they also can be the assemblys that tape frame is removable or porous is removable; 8) hole shape of micropore can have multiple geometric configuration, as square, circular, oval, many polygon; 9) micropore has higher adsorptive power.Because multiple shape can be arranged at the bottom of micropore and microflute, as V or U font, flat perpendicular type or C font; The sidewall of micropore and microflute and lower surface can be level and smooth, also can be rough to increase its surface area; 10) this plate for detecting immunity is water white, and light-permeable or light tight also can have multiple color (as the black and white reddish yellow bluish-green etc.); 11) generally leave a certain size zone at an end of microwell plate, so that each piece high density microwell plate is carried out mark or prints bar shaped identification code etc.
This plate for detecting immunity is mainly used in the solid phase adsorption carrier in the immune microwell array detection, also can be used for making nucleic acid molecular hybridization, microorganism (bacterium, virus etc.), the solid phase adsorption carrier in the detection of environmental monitorings such as enzymatic activity molecule, medical science, biology etc.; On microwell plate, can be according to certain arrangement sequence, in micropore (groove), add respectively or point adds several, tens kind even tens thousand of kind different nucleic acid, proteinase or the bioactive molecules such as specific antibody, antigen relevant with detected material of certain volume; Under certain conditions, above-mentioned bioactive molecule is adsorbable or be solidificated on the bottom or sidewall of high density microwell plate, and is used for the check and analysis of immune microarray.
Claims (10)
1, a kind of plate for detecting immunity comprises plate body (1), micropore (2), it is characterized in that: be provided with microflute (3) between the micropore (2), microflute (3) and micropore (2) form chain.
2, plate for detecting immunity according to claim 1 is characterized in that: the degree of depth of micropore (2) is the 0-2 millimeter, when the degree of depth of micropore near zero the time, micropore be distributed as high density.
3, plate for detecting immunity according to claim 1 is characterized in that: the width of microflute (3) can equate with the aperture of micropore (2), promptly only occurs the microflute that links up on plate body.
4, according to claim 1 or 2 or 3 described plate for detecting immunity, it is characterized in that: be furnished with lid (4) on plate body (1), lid (4) forms confined space with micropore (2) and microflute (3).
5, plate for detecting immunity according to claim 1 and 2 is characterized in that: the arrangement of micropore (2) can have multiple mode, can be as a whole, and grouping or evenly distributed also can be the assembly that tape frame is removable or porous is removable.
6, plate for detecting immunity according to claim 4 is characterized in that: the arrangement of micropore (2) can have multiple mode, can be as a whole, and grouping or evenly distributed also can be the assembly that tape frame is removable or porous is removable.
7, a kind of plate for detecting immunity comprises plate body (1), micropore (2), it is characterized in that: be furnished with lid (4) on plate body (1), form the confined space of microgap (6) between lid (4) and the plate body (1).
8, a kind of plate for detecting immunity comprises plate body (1), micropore (2), it is characterized in that: micropore (2) be arranged as packet type, the last plane of every group of micropore is lower than the last plane of plate body (1) and forms reaction tank (5).
9, according to claim 1 or 7 or 8 described plate for detecting immunity, it is characterized in that: the bottom and the sidewall of micropore (2) and microflute (3) can be rough and uneven in surface shape, as zigzag, cone-shaped.
10, according to claim 1 or 7 or 8 described plate for detecting immunity, it is characterized in that: the material of plate body (1) is transparent or opaque.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01207812 CN2478109Y (en) | 2001-03-09 | 2001-03-09 | Immunity pick-up plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01207812 CN2478109Y (en) | 2001-03-09 | 2001-03-09 | Immunity pick-up plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2478109Y true CN2478109Y (en) | 2002-02-20 |
Family
ID=33628110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01207812 Expired - Fee Related CN2478109Y (en) | 2001-03-09 | 2001-03-09 | Immunity pick-up plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2478109Y (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005012561A1 (en) * | 2003-08-01 | 2005-02-10 | Capital Biochip Company Ltd. | Microarray devices having controllable reaction volume |
| CN100414296C (en) * | 2002-06-14 | 2008-08-27 | 赵翀 | Embedded high-pass three-dimensional biological detecting technique and agent box |
| CN101861383A (en) * | 2007-08-02 | 2010-10-13 | 富山县 | Cell screening method |
| CN102313802A (en) * | 2010-07-09 | 2012-01-11 | 上海荣盛生物药业有限公司 | Component for detecting antigen or antibody and application of component |
| CN103018434A (en) * | 2012-12-05 | 2013-04-03 | 北京鸿天志远科技有限公司 | Multi-index detecting device, kit and application thereof |
| CN106896050A (en) * | 2017-04-10 | 2017-06-27 | 遵义医学院附属医院 | A kind of blood counting chamber |
| CN109926114A (en) * | 2019-04-16 | 2019-06-25 | 孙嘉淦 | A kind of detection microplate and high flux fluid detection device |
-
2001
- 2001-03-09 CN CN 01207812 patent/CN2478109Y/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100414296C (en) * | 2002-06-14 | 2008-08-27 | 赵翀 | Embedded high-pass three-dimensional biological detecting technique and agent box |
| JP2007506933A (en) * | 2003-08-01 | 2007-03-22 | キャピタルバイオ コーポレーション | Microarray device with controllable reaction volume |
| US8293519B2 (en) | 2003-08-01 | 2012-10-23 | Capitalbio Corporation | Microarray devices having controllable reaction volume |
| WO2005012561A1 (en) * | 2003-08-01 | 2005-02-10 | Capital Biochip Company Ltd. | Microarray devices having controllable reaction volume |
| CN101861383B (en) * | 2007-08-02 | 2015-11-25 | 富山县 | Cell screening method |
| CN101861383A (en) * | 2007-08-02 | 2010-10-13 | 富山县 | Cell screening method |
| CN102313802A (en) * | 2010-07-09 | 2012-01-11 | 上海荣盛生物药业有限公司 | Component for detecting antigen or antibody and application of component |
| CN102313802B (en) * | 2010-07-09 | 2014-04-23 | 上海荣盛生物药业有限公司 | Component for detecting antigen or antibody and application of component |
| CN103018434A (en) * | 2012-12-05 | 2013-04-03 | 北京鸿天志远科技有限公司 | Multi-index detecting device, kit and application thereof |
| CN103018434B (en) * | 2012-12-05 | 2016-01-20 | 博源诺信（北京）生物科技有限责任公司 | A kind of multiple determination device and a kind of kit, and application |
| CN106896050A (en) * | 2017-04-10 | 2017-06-27 | 遵义医学院附属医院 | A kind of blood counting chamber |
| CN106896050B (en) * | 2017-04-10 | 2023-08-29 | 遵义医学院附属医院 | Blood cell counting plate |
| CN109926114A (en) * | 2019-04-16 | 2019-06-25 | 孙嘉淦 | A kind of detection microplate and high flux fluid detection device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN2478109Y (en) | Immunity pick-up plate | |
| CN207586245U (en) | Centrifugal type microfludic chip | |
| ES2072399T3 (en) | APPARATUS FOR TAKING AND TESTING FLUID SAMPLES. | |
| CN1330154A (en) | Cell microarray chip and its preparing process | |
| CN108273454A (en) | A kind of method that nanoliter level microlayer model merges in small-sized reaction tube | |
| US4235687A (en) | Measuring cell for micro-assays, comprising membrane electrodes | |
| CN103834554A (en) | Microfluidic microbial culture detection chip | |
| ES2421257T3 (en) | Method and apparatus for simultaneous testing of several analytes with an internal control | |
| CN2390207Y (en) | Microfluid biological chip analysis plate | |
| CN202562928U (en) | Visualized protein chip device | |
| CN108823092A (en) | Liquid drop chip nucleic acid analysis system and analysis method thereof | |
| CN219117415U (en) | Portable nucleic acid extraction detection device | |
| Pokrzywnicka et al. | A multicommutated tester of bioreactors for flow analysis | |
| CN116689051A (en) | Micro-fluidic chip for bacteria identification and use method | |
| CN208350437U (en) | A kind of environment measuring water sample acquisition device | |
| US5869329A (en) | Blood culture vial or bottle having an agitation paddle | |
| KR870009032A (en) | Detection of Microorganisms in Samples Containing Nucleic Acids | |
| CN202730111U (en) | Biochemical culturing and identifying device | |
| CN208443738U (en) | A kind of new structural SERS substrate | |
| CN209810203U (en) | Micro-fluidic chip with color development detects function | |
| CN2554170Y (en) | Bio-chip for detecting pylorus spirochete bacillus index | |
| JP6959644B2 (en) | Electrophoresis fully automatic processing device and its method | |
| CN2441810Y (en) | Biological chip and integrated biological chip | |
| CN2554176Y (en) | Bio-chip for detecting rheumatic disease index | |
| CN2775659Y (en) | Plastic chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |