CN202562928U

CN202562928U - Visualized protein chip device

Info

Publication number: CN202562928U
Application number: CN2012200424128U
Authority: CN
Inventors: 许丹科; 李周敏
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-02-09
Filing date: 2012-02-09
Publication date: 2012-11-28
Anticipated expiration: 2022-02-09

Abstract

The invention discloses a utility model of a visualized protein chip device. The visualized protein chip device consists of a chip substrate, and a set of sample cells with inward depression. A step shaped extending edge is provided on one side edge of the chip substrate for facilitating operation. The chip substrate is subjected to surface treatment, has high adsorptivity on biorecognition molecules, and is transparent. The utility model realizes high-throughput detection of multiple biological indexes in biological sample. The sample cells are independent from one another to reduce cross contamination and reduce consumption of sample and reagent. Four utility models can be assembled together to form the shape of a standard 96-well plate, which facilitates automatic sample injection and cleaning, and increases detection efficiency. The utility model can be used for visualized scanning using a common flat CCD scanner for collecting image. It can be used in the fields of antibody screening, drug screening, food safety test, environmental small molecule test, and in vitro clinical diagnosis and monitoring.

Description

A kind of visible protein chip device

Technical field

The utility model relates to biochip and bioanalysis equipment, or rather, relates to a kind of visible protein matter chip apparatus that is suitable for many indexs, the parallel detection of various article.

Background technology

Utilizing antigen, antibody response is one of important method of medical diagnosis on disease, has obtained widespread use clinically.But, present immunological detection method, as: ELISA, emitting isotope mark, golden mark, fluorescence, chemiluminescence, time-resolved fluorescence etc., the overwhelming majority is that single index detects, and that is to say that single sample can only detect the information of single index.Developing into of biochip technology realizes the rapid of clinical diagnosis, parallelization, and high pass quantizes detection provides possibility, and detection efficiency has obtained bigger raising, and detection time, cost also can significantly reduce thereupon.

Biological micro-array chip is meant that methods such as adopting micro-sampling is immobilized onto holder (like microslide in an orderly manner with the biomolecule sample; Plastics, carriers such as film) surface, form intensive molecules align; Then with the biological sample to be measured of mark in target molecule hatching reaction; Through specific instrument, as laser confocal scanning or charge-coupled device (CCD) to the intensity of reaction signal carry out fast, walk abreast, check and analysis efficiently, thereby the content of target molecule in the judgement sample.Same chip can make up a plurality of sample cells, and each sample cell can detect people's duplicate samples, and thousands of hundreds of antigen can be fixed in each sample cell inside, and antibody or other biological capture molecules array carry out many targets and detect simultaneously.In view of the above, chip can be realized parallelization, the flux check and analysis of various article, many indexs.

Yet the biochip product that makes things convenient for actual sample to use that utilizes above-mentioned principle to produce also must solve following problem:

1. for realizing the analyzing and testing of a plurality of samples on same the chip, separate each biological sample reaction tank, the immune response of different samples can be carried out separately.

2. reaction tank will make things convenient for application of sample, operations such as cleaning.

3. prevent cross pollution such as reagent seepage in the course of reaction.

4. reacted chip will be convenient to scanning, carries out Flame Image Process.

5. reaction back sample result can be through the color of coloration method array of display point, and the array point in each sample cell can detect through the plain scan appearance, can realize the fast detecting of actual sample.

Therefore, this area presses for new, easy to use, the with low cost visual micro-array biochip of exploitation.

The utility model content

The utility model purpose: the utility model technical matters to be solved is the deficiency to prior art, and a kind of visible protein chip device is provided.

In order to solve the problems of the technologies described above, the utility model discloses a kind of visible protein chip device, comprise the sheet base, on the sheet base, be provided with one group of sample cell that caves inward, a side of said base is provided with the sheet base extension limit of step shape, is convenient to operation.Said base be through surface treatment, and biological identification molecule is had high absorption and for transparent.

In the utility model, said base is the sheet base that any one materials such as high molecular polymer, glass and plastics are processed.

In the utility model, said basic xsect is rectangle.

In the utility model, said one group of sample cell comprises 24 sample cells, is 3 row, the distribution of 8 column array shapes.Separate between each sample cell, the bottom is the plane, and identical with upper end xsect size.

In the utility model, said sample cell cross sectional shape is any one in circular, square, sexangle or the ellipse, and the cross-sectional area of sample cell is less than or equal to the cross-sectional area of opening part.

In the utility model, be provided with microarray zones such as biological identification molecule in the sample cell.Microarray zones such as biological identification molecule are arranged in the said spill cavernous structure.The available ordinary flat ccd scanner in said protein microarray zone scans images acquired.

Beneficial effect: in the utility model, be fixed with a plurality of biomolecule recognizates in the sample cell, can realize the purpose of a plurality of Biological indicators in the high throughput testing biological specimen like this.Simultaneously 4 chip blocks one-tenth 96 orifice plate shapes capable of being combined and combine the design of a plurality of sample cells can once be analyzed large batch of biological specimen, really realize the flux testing goal, can realize that various article, the parallelization of many indexs handle.Each sample cell is separate, reduces cross pollution, and sample and reagent consumption are little.Four the utility model one-tenth standard 96 orifice plate shapes capable of being combined also are convenient to automatic application of sample, operations such as cleaning, and simple and feasible has improved detection efficiency greatly.The utility model can carry out visual scanning, images acquired with the ordinary flat ccd scanner.The utility model can be adaptable across antibody screening, drug screening, food safety detection, the medium and small Molecular Detection of environment, and field such as external clinical diagnosis monitoring.

Description of drawings

Below in conjunction with accompanying drawing and embodiment the utility model is done specifying further, the above-mentioned and/or otherwise advantage of the utility model will become apparent.

Fig. 1 is the plan structure synoptic diagram of embodiment 1.

Fig. 2 is the cut-open view at the place, long limit of embodiment 2.

Embodiment

As depicted in figs. 1 and 2, the visual micro-array biochip of the utility model is that rectangular pieces base 1 is formed by an xsect through the high absorption of surface treatment.Said plate substrate material is plastics, microslide, and transparent materials such as film are preferably plastics, compares with aldehyde slide, greatly reduces material cost.

This sheet base length overall 76.2mm, wide is 25.4mm, height is 2mm.Minor face place, lower end has a notch cuttype to extend recessed 3, and this recessed long is the wide 25.4mm of being of said base, and recessed wide not special restriction is generally 0.5-2.5mm, is preferably 1-2mm, and height is not restriction especially also, is generally 0.1-2mm, is preferably 0.5-1.5mm.

Frame around the sheet base, zone line have 24 matrix sample cells 2 of horizontal three perpendicular eight array arrangements, and being shaped as of said matrix sample cell is circular, square, sexangle, ellipse etc., is preferably circle.

Said sample cell bottom is the plane, and identical with upper end xsect size, and diameter is 3-8mm, is preferably 5-7mm, and the matrix sample cell degree of depth is 0.1-2mm, is preferably 0.5-1.5mm; Each sample cell is separate, and minor increment is 1-6mm between two holes, is preferably 2-4mm, and two sample cell centre distances are 9mm; Sample cell is preferably 2-4mm apart from sheet base top 1-4mm, is 1-4mm apart from sheet basal part concave edge, is preferably 2-3mm, respectively is 0.7mm apart from both sides.Sample cell inside is the protein microarray zone, and 2-1000 protein spots sampling point contained in described each microarray zone.The also not special restriction of sample volume in each reaction tank is generally the 10-100 microlitre, is preferably the 20-50 microlitre.

Four said chip apparatus one-tenth standard 96 orifice plate shapes capable of being combined, therefore biological specimen also can add in each sample cell of combination back chip one to one in 96 orifice plates, is convenient to automatic application of sample on the one hand; Operations such as cleaning realize once adding simultaneously, get a plurality of to be measured or reaction samples; Also can be omitted in the sample mark in the transfer process on the other hand; Accelerate detection procedure, the operation simple and feasible has improved detection efficiency greatly.And the result can carry out visual detection, greatly reduces the detection cost.

Embodiment 1

Present embodiment prepares and has detected the antibiotic residue detection chip of the utility model.

As depicted in figs. 1 and 2, present embodiment comprises the transparent strip plastic base 1 through the high absorption of surface treatment, length overall 76.2mm, and wide is 25.4mm, height is 2mm.Minor face place, lower end has one recessed 3, and this recessed long is 25.4mm, and wide is 1.5mm, and height is 1mm.Frame around the sheet base, zone line are 24 matrix cylindrical sample ponds 2 of horizontal three perpendicular eight array arrangements, and its diameter is 6mm, and the degree of depth is 1mm; Each sample cell is separate, and minor increment is 3mm between two holes, and two sample cell centre distances are 9mm; Sample cell is 2.7mm apart from sheet base top 3mm apart from sheet basal part concave edge, respectively is 0.7mm apart from both sides.Sample cell inside is the protein microarray zone, and first is contained in described each microarray zone, second, the third, fourth four kinds of antibiotic artificial antigens, fixedly rear encloseds.

Detection method:

1. the sample to be checked and corresponding antibodies 50 microlitres that add certain dilution are in reaction tank, and (doing blank simultaneously, the positive control hole) hatched 30min for 37 ℃.

2.PBST wash 3 times, each 15min claps and does.

3. it is anti-to add gold mark two in each reaction tank, hatches 30min for 37 ℃.

4.PBST wash 3 times, each 15min, deionized water wash is clapped and is done.

5. add silver in each reaction tank and strengthen colour developing liquid, the lucifuge reaction.

6. chip is put into flat bed scanner and carry out visual detection.

7. computer image analysis, the bright spot on the image, expression is negative, and intensity is high more, representes that content of material to be checked is high more.

The result: the present embodiment chip apparatus not only makes things convenient for application of sample, cleaning; And can prevent in the course of reaction cross pollution such as reagent seepage, the chip after reaction finishes is convenient to the scanning of ordinary flat ccd scanner, carries out Flame Image Process; Greatly reduce the detection cost; Can realize various article of sample, the parallelization of many indexs is handled, and easy to operation.

The utility model provides a kind of thinking and method of visible protein chip device; The method and the approach of concrete this technical scheme of realization are a lot, and the above only is the preferred implementation of the utility model, should be understood that; For those skilled in the art; Under the prerequisite that does not break away from the utility model principle, can also make some improvement and retouching, these improvement and retouching also should be regarded as the protection domain of the utility model.The all available prior art of each ingredient not clear and definite in the present embodiment realizes.

Claims

1. a visible protein chip device is characterized in that, comprises the sheet base, on the sheet base, is provided with one group of sample cell that caves inward, and a side of said base is provided with the sheet base extension limit of step shape.

2. a kind of visible protein chip device according to claim 1 is characterized in that said base is the sheet base that any one materials such as high molecular polymer, glass and plastics are processed.

3. a kind of visible protein chip device according to claim 1 is characterized in that said basic xsect is rectangle.

4. a kind of visible protein chip device according to claim 1 is characterized in that, said one group of sample cell comprises 24 sample cells, is 3 row, the distribution of 8 column array shapes.

5. a kind of visible protein chip device according to claim 1; It is characterized in that; Said sample cell cross sectional shape is any one in circular, square, sexangle or the ellipse, and the cross-sectional area of sample cell is less than or equal to the cross-sectional area of opening part.