CN202201884U

CN202201884U - Kit for cell biotechnology

Info

Publication number: CN202201884U
Application number: CN 201120276982
Authority: CN
Inventors: 王开道
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-08-02
Filing date: 2011-08-02
Publication date: 2012-04-25
Anticipated expiration: 2021-08-02

Abstract

The utility model relates to a kit for a cell biotechnology. The kit reflects large information content, is simple to use, has a low cost and can simultaneously analyze the distribution and expression regulation of biomolecules such as a gene, a protein, an antigen and the like in a plurality of different histiocytes or cell samples. A traditional cell chip-stretching test has a problem that two or more than two different histiocytes are very difficult to simultaneously carry out the test as the different histiocytes are difficult to distinguish. According to the utility model, a cell dot matrix setting is used for arranging the cells of different tissues or species origins on a same glass slide in the forms of dots in sequence, and thus the problem can be solved. The kit for the cell biotechnology comprises a plate body, a micropore, a cell chip, a microflow path and a plate cover, and is characterized in that the plate body can be made of glass, ceramic, metal material, latex or silica gel material, and processed to be a square, rectangular, circular-disc shaped flat plate or a thin sheet.

Description

A kind of cellular biological technique is used test kit

Technical field

The utility model relates to a kind of cellular biological technique and uses test kit.Containing much information of this test kit reaction, use is simple, cost is low, simultaneously distribution and the expression regulations of biomolecules in multiple different tissues cell or cell sample such as analyzing gene, albumen and antigen; Also can be used for the high-flux parallel check and analysis of the multiple different compositions such as chemical substance, biomolecules, mikrobe or cell of sample.

Background technology

Cell and clone are the most frequently used experiment made on the living materials of biology, medical science, pharmacy and association area, often are used to tissue distribution, cellular localization and the expression regulation and the drug screening etc. of biomolecules such as gene, albumen.In the case, traditional cell shop sheet is tested existing problem and is: to surpassing 2 kinds or above different tissues cell, owing to be difficult to distinguish, be difficult to carry out simultaneously.The utility model adopts the setting of cell dot matrix, with the cell of different tissues or source of species, is point-like and is arranged in an orderly manner on same the slide glass, then can solve the above problems.

The utility model content

The purpose of the utility model provides a kind of easy sensitivity, fast in time, the device that can be simultaneously multiple different tissues cell or material composition be carried out check and analysis.

The utility model is with monolayer growth and merge different cells in blocks or the clone point-like is arranged on the sheet base; Form the cell dot matrix, so that to the having or not of multiple heterogeneities such as chemical substance, bio-target molecule, cell and mikrobe unknown in the sample, character, content, source; Or the device of the bioanalysis that biomolecules such as known nucleic acid, albumen and antigen are identified simultaneously, analyzed or detect in tissue and distribution and location etc. in the cell.The reaction result of each cell point can be used instrument record analyses such as range estimation, microscope or scanner; According to arrangement position and the order of cell point in the cell sheet, accomplish sample amplifying nucleic acid, albumen and the equimolecular detection of antigen and histiocytic distribution, expression regulation.This utility model provides a kind of cellular biological technique to use test kit.But the sample composition that each point is caught in the reagent pair cell sheet that utilizes in the test kit to be provided does further analysis.This test kit has not only been simplified the operation steps that same sample carries out multifactor check and analysis greatly; Shortened the running time; Reduced working strength; More increased particularity, sensitivity, circulation ratio and the comparability of detected result, but also can do further different the analysis to multiple different material composition in the test sample simultaneously, used more convenient; Operate fasterly, and can be widely used in technical study fields such as environment measuring, medical diagnosis on disease, new drug development, vaccine research, genome and protein science.

The utility model comprises plate body, micropore, cell sheet, microfluidic circuit, plate lid composition; It is characterized in that: plate body can be glass, pottery, metallic substance, latex or silica gel material, is processed to the flat board or the thin slice of square, rectangle, collar plate shape.

The characteristic of the utility model also is: microfluidic circuit is made up of structures such as inlet opening, fluid hole, micropore, microflute, sealing-ring, peristaltic tubes, can lay respectively at plate body or plate to cover, and also can all be positioned at plate body or plate covers; Microfluidic circuit links to each other with micropore through microtubule, and communicates with the external world through inlet opening and fluid hole;

The characteristic of the utility model also is: micropore communicates with the external world through microfluidic circuit or forms closed circuit.

The characteristic of the utility model also is: peristaltic tube is between inlet opening and fluid hole; Microfluidic circuit is connected into closed circuit; Liquid such as sample and affinity tag can be circulated, with the interaction that promotes sample and affinity tag etc. and cell and biomolecules thereof etc. and combine in microfluidic circuit;

The utlity model has following advantage: 1, contain much information.On the sheet base of a slide glass size; Arrange the cell of tens of even hundreds of different qualities; An experimental implementation just can obtain the data informations such as cytology location, tissue distribution and expression regulation of assay all cells on the sheet base, or accomplishes qualitative, the quantitative parallel parsing of the high-throughput of hundreds and thousands of kinds of different checking matters.2, be easy to preparation, cost is low.3, easy and simple to handle, save sample and reagent.4, tradition and existing detection method good reproducibility, excellent characteristics such as accuracy height, good reliability have been kept.5, the result is easy to analyze.6, purposes is more extensive.The utility model not only can be used for researchs such as tissue/cells such as gene, albumen and antigen location and expression regulation, can also directly be used for the research and development of modern life sciences such as exploitation and proteomics of diagnosis, the new drug of disease; And can also cut capture technique by laser capture microdissection to interested cell submicroscopic structure, directly carry out mass spectroscopy; The data informations such as different elution requirements of the binding substances that obtains can also be used for the separation and purification of follow-up target molecule etc.

Description of drawings

Fig. 1 is that a kind of biotechnology of the utility model is used the test kit synoptic diagram.

Fig. 2 has micropore, microfluidic circuit and cell chip architecture synoptic diagram for a kind of biotechnology of the utility model with test kit.

Embodiment:

In conjunction with accompanying drawing 1 and accompanying drawing 2, further specify as follows:

Visible referring to accompanying drawing 1, accompanying drawing 2; The plate body 3 of the utility model 1 is provided with some micropores 5, and micropore 5 can become different shape by the demands of different spot distribution, the inner cell sheet 2 of placing; One end of plate body 3 is provided with inlet opening 7 and fluid hole 8, with peristaltic tube 11 UNICOMs; Inlet opening 7 and first micropore 5 UNICOMs, fluid hole 8 and end micropore 5 UNICOM, by microflute 6 UNICOM successively, plate lid 4 is arranged at micropore 5 tops between the micropore 5, and microfluidic circuit 9 is formed at the top of closed porosity 5 and microflute 6.Microfluidic circuit 9 is made up of structures such as inlet opening 7, fluid hole 8, microflute 6, micropore 5, sealing-ring 10, peristaltic tubes 11, and injecting institute through the cell sheet in 9 pairs of micropores of microfluidic circuit 52 must reagent.

Claims

1. one kind cellular biological technique comprises plate body, micropore, cell sheet, microfluidic circuit, Ban Gai with test kit; It is characterized in that: plate body can be glass, pottery, metallic substance, latex or silica gel material, is processed to the flat board or the thin slice of square, rectangle, collar plate shape.

2. a kind of cellular biological technique according to claim 1 is used test kit; It is characterized in that: microfluidic circuit is made up of structures such as inlet opening, fluid hole, micropore, microflute, sealing-ring, peristaltic tubes; Can lay respectively at plate body or plate covers, also can all be positioned at plate body or plate covers; Microfluidic circuit links to each other with micropore through microtubule, and communicates with the external world through inlet opening and fluid hole;

3. a kind of cellular biological technique according to claim 1 is used test kit, it is characterized in that: micropore communicates with the external world through microfluidic circuit or forms closed circuit.

4. a kind of cellular biological technique according to claim 2 is used test kit, it is characterized in that: peristaltic tube connects into closed circuit with microfluidic circuit between inlet opening and fluid hole.